Irma Ximena Barbosa Sanchez | Universidad del Tolima Colombia (original) (raw)

Uploads

Papers by Irma Ximena Barbosa Sanchez

O cobre e um microelemento essencial para os mamiferos, porem, quando ingerido em grande quantida... more O cobre e um microelemento essencial para os mamiferos, porem, quando ingerido em grande quantidade, pode levar os animais a intoxicarem-se. Existe diferente susceptibilidade a intoxicacao por cobre entre as especies domesticas, e ate mesmo entre racas dentro da propria especie. Os ovinos sao os mais propensos a desenvolverem a enfermidade, especialmente a de evolucao cronica, o que causa grandes perdas economicas. A maioria dos ovinos acometidos e proveniente de manejo intensivo, os quais recebem dietas ricas em concentrados energeticos, e a ocorrencia mais comum se da quando estes animais ingerem misturas minerais destinadas a bovinos. Entre os sinais clinicos destacam-se a ictericia e a hemoglobinuria. O diagnostico e realizado por meio do historico, exame clinico, alteracoes macro e microscopicas, alem da determinacao dos niveis cupricos no figado e/ou rins. A terapia pode ser eficiente se for instituida precocemente apos o inicio da hemoglobinuria, sendo utilizado o tetratiomol...

A Erliquiose monocitica canina e uma zoonose causada pelaEhrlichia canis, bacteria Gram negativa ... more A Erliquiose monocitica canina e uma zoonose causada pelaEhrlichia canis, bacteria Gram negativa de distribuicao mundial. Alguns caes com erliquiose se tornam portadoresassintomaticos enquanto outros desenvolvem uma doencaaguda com morte rapida. A apoptose pode ser importante naeliminacao de patogenos intracelulares, podendo, nas infeccoes porEhrlichiasp. eAnaplasmasp., ocorrer modulacao daapoptose celular para prolongar a sobrevivencia desses organismos, permitindo sua proliferacao e disseminacao, atuando como um cavalo de Troia inibindo a resposta imune. Neste contexto, a apoptose pode contribuir indistintamente para a progressao ou regressao das lesoes. Para avaliacao do papel da apoptose na erliquiose monocitica canina, sete caes foram distribuidos em dois grupos. No Grupoinoculado, realizou-se a infeccao por via intravenosa comsangue infectado com E. Canis (isolado Jaboticabal), sendorealizada a inoculacao com PBS esteril nos animaispertencentes ao Grupo Controle. Semanalmente ...

Revista U.D.C.A Actualidad & Divulgación Científica, 2018

Platelet-rich plasma (PRP) is the portion of the blood plasma which contains a platelet concentra... more Platelet-rich plasma (PRP) is the portion of the blood plasma which contains a platelet concentration above basal levels that stimulate tissue repair by means to growth factors. The aimed of this research was to evaluate the impact of PRP on skin repair upon epidermal surgical lesions. Using a scalpel, a clean epidermal incision of 5cm length was made in the interscapular region of New Zealand rabbits (n=8), and then divided in two groups. Rabbits from the group 1 (n=4) were untreated (control group), while group 2 received PRP on the surgical wound. Autologous PRP was obtained from whole blood drawn by jugular vein puncture, collected with sodium citrate anticoagulant solution, and centrifuged at 120g x 5min. One milliliter was obtained of the phase superior to the buffy coat. Biopsies from the injured tissue were collected on days 3, 7, 14 and 21 post-surgeries, and submitted to Hematoxylin-Eosin and Masson's Trichrome staining. In conclusion, the platelets separation method allows to obtaining plasma with higher platelets concentration and reduced number of leukocytes. Additionally, the results show that therapeutic PRP administration on surgically-induced skin injury has effect on the histological features of tissue healing compared with the control group .

Arquivos Brasileiros de Oftalmologia, 2015

Microvascular Research, 2014

The increased prevalence of diabetes worldwide is associated with increasing numbers of diabetic ... more The increased prevalence of diabetes worldwide is associated with increasing numbers of diabetic individuals receiving synthetic matrices and biomedical implants to repair and/or replace biological tissues. This therapeutic procedure invariably leads to adverse tissue healing (foreign body reaction), thus impairing the biomedical device function of subcutaneous implants. However, the influence of diabetes on abnormal tissue healing in intraperitoneal implants is unclear. We investigated key components of foreign body reactions in diabetic rats. Polyether-polyurethane sponge discs were placed intraperitoneally in rats previously injected with streptozotocin for induction of diabetes and in non-diabetic rats. Implants removed 10 days after implantation were assessed by determining the components of the fibrovascular tissue (angiogenesis, inflammation, and fibrogenesis). In implants from diabetic rats, fibrous capsule thickness and fibrovascular tissue infiltration (hematoxylin & eosin and picrosirius staining) were reduced in comparison with implants from non-diabetic rats. Hemoglobin (Hb) content (vascular index) and VEGF levels (pro-angiogenic cytokine) were increased after diabetes. However, the number of vessels (H&E and CD31-immunostaining) in the fibrovascular tissue from diabetic rats was decreased when compared with vessel numbers in implants from non-diabetic animals. Overall, all inflammatory parameters (macrophage accumulation-NAG activity; TNF-α and MCP-1 levels) increased in intraperitoneal implants after diabetes induction. The pro-fibrogenic cytokine (TGFβ-1) increased after diabetes, but collagen deposition remained unaltered in the implants from diabetic rats. These important diabetes-related changes (increased levels of pro-inflammatory and angiogenic and fibrogenic cytokines) in peritoneal implant healing provide an insight into the mechanisms of the foreign body response in the diabetic environment in rats.

Revista Colombiana De Ciencia Animal, Apr 9, 2015

ABSTRACT Proper recognition of the different stages of the estrous cycle constitutes a useful dia... more ABSTRACT Proper recognition of the different stages of the estrous cycle constitutes a useful diagnostic tool for monitoring the reproductive system in the bitch. Several approaches methods for the diagnosis of the ovulation time are used for clinical purposes, including identification of changes in the external genitals, performing serial vaginal smears, microscopic evaluation of salivary smear, or the serial measurement of serum progesterone levels among others. This document describes the basic principles for monitoring the reproductive system in the bitch, as well as the main pathologies that affect this system. It concludes that the identification of behavioral, anatomical, hormonal and cellular changes observable throughout the estrous cycle are sensitive methods for monitoring reproductive system in the bitch.

Arquivos Brasileiros de Oftalmologia, 2015

Humans and animals often develop ulcerative keratitis , which leads to discomfort and pain due to... more Humans and animals often develop ulcerative keratitis , which leads to discomfort and pain due to the activation of nociceptors predominantly located in the superficial layer of the cornea . Nalbuphine is an opioid kappa agonist/mu antagonist that produces moderate to severe analgesia (3) and has been used in premedication, surgery, and the postoperative period of obstetric and pediatric practices focused on controlling pain associated with burns (4) . Nalbuphine activity depends on the dose, application site, and sex of the patient . Studies on the analgesic effects of nalbuphine on the cornea have shown that instillation reduces the corneal sensitivity to touch in dogs . In contrast, nalbuphine has been demonstrated to sometimes cause damage to the corneal epithelium and stromal fibroblasts . It is unclear if nalbuphine can impair the expression of enzymes and growth factors involved in corneal wound healing.

Ciência Rural, 2012

Recebido para publicação 03.11.11 Aprovado em 04.04.12 Devolvido pelo autor 04. 06.12 CR-6282 RES... more Recebido para publicação 03.11.11 Aprovado em 04.04.12 Devolvido pelo autor 04. 06.12 CR-6282 RESUMO A Erliquiose canina é uma zoonose causada pela Ehrlichia canis, bactéria Gram negativa de distribuição mundial. Alguns cães com erliquiose se tornam portadores assintomáticos enquanto outros desenvolvem uma doença aguda com morte rápida. A apoptose pode ser importante na eliminação de patógenos intracelulares, podendo, nas infecções por Ehrlichia sp. e Anaplasma sp., ocorrer modulação da apoptose celular para prolongar a sobrevivência desses organismos. Para avaliação do papel da apoptose na erliquiose canina, sete cães foram distribuídos em dois grupos. No Grupo inoculado, realizou-se a infecção por via intravenosa com sangue infectado com E. canis (isolado Jaboticabal), sendo realizada a inoculação com PBS estéril nos animais pertencentes ao Grupo Controle. Semanalmente e até 35 dias pós-inoculação, amostras de sangue foram coletadas e submetidas a n-PCR e reação de imunofluorescência (RIFI) para confirmação da infecção. No 36 o dia pós-inoculação, os animais foram eutanasiados, sendo as amostras de baço, linfonodo, rim e fígado coletadas e processadas para as técnicas de TUNEL e imunohistoquímica (Bcl-2, Bax). Verificou-se pela n-PCR que os animais inoculados se tornaram positivos para E. canis a partir da segunda semana. Pela RIFI, verificou-se animais com sorologia positiva a partir da terceira semana pós-inoculação. No grupo controle, todos os testes realizados foram negativos para E. canis. Apesar da reação de TUNEL mostrar maior incidência de apoptose no Grupo Inoculado, sendo o baço e os linfonodos os órgãos que apresentaram maior marcação, os resultados da imunohistoquímica para Bcl-2 e Bax indicam que a via intrínseca de apoptose não é importante nos órgãos analisados.

O cobre e um microelemento essencial para os mamiferos, porem, quando ingerido em grande quantida... more O cobre e um microelemento essencial para os mamiferos, porem, quando ingerido em grande quantidade, pode levar os animais a intoxicarem-se. Existe diferente susceptibilidade a intoxicacao por cobre entre as especies domesticas, e ate mesmo entre racas dentro da propria especie. Os ovinos sao os mais propensos a desenvolverem a enfermidade, especialmente a de evolucao cronica, o que causa grandes perdas economicas. A maioria dos ovinos acometidos e proveniente de manejo intensivo, os quais recebem dietas ricas em concentrados energeticos, e a ocorrencia mais comum se da quando estes animais ingerem misturas minerais destinadas a bovinos. Entre os sinais clinicos destacam-se a ictericia e a hemoglobinuria. O diagnostico e realizado por meio do historico, exame clinico, alteracoes macro e microscopicas, alem da determinacao dos niveis cupricos no figado e/ou rins. A terapia pode ser eficiente se for instituida precocemente apos o inicio da hemoglobinuria, sendo utilizado o tetratiomol...

A Erliquiose monocitica canina e uma zoonose causada pelaEhrlichia canis, bacteria Gram negativa ... more A Erliquiose monocitica canina e uma zoonose causada pelaEhrlichia canis, bacteria Gram negativa de distribuicao mundial. Alguns caes com erliquiose se tornam portadoresassintomaticos enquanto outros desenvolvem uma doencaaguda com morte rapida. A apoptose pode ser importante naeliminacao de patogenos intracelulares, podendo, nas infeccoes porEhrlichiasp. eAnaplasmasp., ocorrer modulacao daapoptose celular para prolongar a sobrevivencia desses organismos, permitindo sua proliferacao e disseminacao, atuando como um cavalo de Troia inibindo a resposta imune. Neste contexto, a apoptose pode contribuir indistintamente para a progressao ou regressao das lesoes. Para avaliacao do papel da apoptose na erliquiose monocitica canina, sete caes foram distribuidos em dois grupos. No Grupoinoculado, realizou-se a infeccao por via intravenosa comsangue infectado com E. Canis (isolado Jaboticabal), sendorealizada a inoculacao com PBS esteril nos animaispertencentes ao Grupo Controle. Semanalmente ...

Revista U.D.C.A Actualidad & Divulgación Científica, 2018

Platelet-rich plasma (PRP) is the portion of the blood plasma which contains a platelet concentra... more Platelet-rich plasma (PRP) is the portion of the blood plasma which contains a platelet concentration above basal levels that stimulate tissue repair by means to growth factors. The aimed of this research was to evaluate the impact of PRP on skin repair upon epidermal surgical lesions. Using a scalpel, a clean epidermal incision of 5cm length was made in the interscapular region of New Zealand rabbits (n=8), and then divided in two groups. Rabbits from the group 1 (n=4) were untreated (control group), while group 2 received PRP on the surgical wound. Autologous PRP was obtained from whole blood drawn by jugular vein puncture, collected with sodium citrate anticoagulant solution, and centrifuged at 120g x 5min. One milliliter was obtained of the phase superior to the buffy coat. Biopsies from the injured tissue were collected on days 3, 7, 14 and 21 post-surgeries, and submitted to Hematoxylin-Eosin and Masson's Trichrome staining. In conclusion, the platelets separation method allows to obtaining plasma with higher platelets concentration and reduced number of leukocytes. Additionally, the results show that therapeutic PRP administration on surgically-induced skin injury has effect on the histological features of tissue healing compared with the control group .

Arquivos Brasileiros de Oftalmologia, 2015

Microvascular Research, 2014

The increased prevalence of diabetes worldwide is associated with increasing numbers of diabetic ... more The increased prevalence of diabetes worldwide is associated with increasing numbers of diabetic individuals receiving synthetic matrices and biomedical implants to repair and/or replace biological tissues. This therapeutic procedure invariably leads to adverse tissue healing (foreign body reaction), thus impairing the biomedical device function of subcutaneous implants. However, the influence of diabetes on abnormal tissue healing in intraperitoneal implants is unclear. We investigated key components of foreign body reactions in diabetic rats. Polyether-polyurethane sponge discs were placed intraperitoneally in rats previously injected with streptozotocin for induction of diabetes and in non-diabetic rats. Implants removed 10 days after implantation were assessed by determining the components of the fibrovascular tissue (angiogenesis, inflammation, and fibrogenesis). In implants from diabetic rats, fibrous capsule thickness and fibrovascular tissue infiltration (hematoxylin & eosin and picrosirius staining) were reduced in comparison with implants from non-diabetic rats. Hemoglobin (Hb) content (vascular index) and VEGF levels (pro-angiogenic cytokine) were increased after diabetes. However, the number of vessels (H&E and CD31-immunostaining) in the fibrovascular tissue from diabetic rats was decreased when compared with vessel numbers in implants from non-diabetic animals. Overall, all inflammatory parameters (macrophage accumulation-NAG activity; TNF-α and MCP-1 levels) increased in intraperitoneal implants after diabetes induction. The pro-fibrogenic cytokine (TGFβ-1) increased after diabetes, but collagen deposition remained unaltered in the implants from diabetic rats. These important diabetes-related changes (increased levels of pro-inflammatory and angiogenic and fibrogenic cytokines) in peritoneal implant healing provide an insight into the mechanisms of the foreign body response in the diabetic environment in rats.

Revista Colombiana De Ciencia Animal, Apr 9, 2015

ABSTRACT Proper recognition of the different stages of the estrous cycle constitutes a useful dia... more ABSTRACT Proper recognition of the different stages of the estrous cycle constitutes a useful diagnostic tool for monitoring the reproductive system in the bitch. Several approaches methods for the diagnosis of the ovulation time are used for clinical purposes, including identification of changes in the external genitals, performing serial vaginal smears, microscopic evaluation of salivary smear, or the serial measurement of serum progesterone levels among others. This document describes the basic principles for monitoring the reproductive system in the bitch, as well as the main pathologies that affect this system. It concludes that the identification of behavioral, anatomical, hormonal and cellular changes observable throughout the estrous cycle are sensitive methods for monitoring reproductive system in the bitch.

Arquivos Brasileiros de Oftalmologia, 2015

Humans and animals often develop ulcerative keratitis , which leads to discomfort and pain due to... more Humans and animals often develop ulcerative keratitis , which leads to discomfort and pain due to the activation of nociceptors predominantly located in the superficial layer of the cornea . Nalbuphine is an opioid kappa agonist/mu antagonist that produces moderate to severe analgesia (3) and has been used in premedication, surgery, and the postoperative period of obstetric and pediatric practices focused on controlling pain associated with burns (4) . Nalbuphine activity depends on the dose, application site, and sex of the patient . Studies on the analgesic effects of nalbuphine on the cornea have shown that instillation reduces the corneal sensitivity to touch in dogs . In contrast, nalbuphine has been demonstrated to sometimes cause damage to the corneal epithelium and stromal fibroblasts . It is unclear if nalbuphine can impair the expression of enzymes and growth factors involved in corneal wound healing.

Ciência Rural, 2012

Recebido para publicação 03.11.11 Aprovado em 04.04.12 Devolvido pelo autor 04. 06.12 CR-6282 RES... more Recebido para publicação 03.11.11 Aprovado em 04.04.12 Devolvido pelo autor 04. 06.12 CR-6282 RESUMO A Erliquiose canina é uma zoonose causada pela Ehrlichia canis, bactéria Gram negativa de distribuição mundial. Alguns cães com erliquiose se tornam portadores assintomáticos enquanto outros desenvolvem uma doença aguda com morte rápida. A apoptose pode ser importante na eliminação de patógenos intracelulares, podendo, nas infecções por Ehrlichia sp. e Anaplasma sp., ocorrer modulação da apoptose celular para prolongar a sobrevivência desses organismos. Para avaliação do papel da apoptose na erliquiose canina, sete cães foram distribuídos em dois grupos. No Grupo inoculado, realizou-se a infecção por via intravenosa com sangue infectado com E. canis (isolado Jaboticabal), sendo realizada a inoculação com PBS estéril nos animais pertencentes ao Grupo Controle. Semanalmente e até 35 dias pós-inoculação, amostras de sangue foram coletadas e submetidas a n-PCR e reação de imunofluorescência (RIFI) para confirmação da infecção. No 36 o dia pós-inoculação, os animais foram eutanasiados, sendo as amostras de baço, linfonodo, rim e fígado coletadas e processadas para as técnicas de TUNEL e imunohistoquímica (Bcl-2, Bax). Verificou-se pela n-PCR que os animais inoculados se tornaram positivos para E. canis a partir da segunda semana. Pela RIFI, verificou-se animais com sorologia positiva a partir da terceira semana pós-inoculação. No grupo controle, todos os testes realizados foram negativos para E. canis. Apesar da reação de TUNEL mostrar maior incidência de apoptose no Grupo Inoculado, sendo o baço e os linfonodos os órgãos que apresentaram maior marcação, os resultados da imunohistoquímica para Bcl-2 e Bax indicam que a via intrínseca de apoptose não é importante nos órgãos analisados.