Christopher Noel | University of Toronto (original) (raw)
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Papers by Christopher Noel
JAMA otolaryngology-- head & neck surgery, Jan 23, 2015
Accurate measurement of health state utilities (HU) is the cornerstone for cost-utility analyses ... more Accurate measurement of health state utilities (HU) is the cornerstone for cost-utility analyses and the valuation of quality of life for given health states. Current indirect methods of HU derivation lack face validity for patients with head and neck cancer. The appropriateness of these measures compared with direct methods, such as the standard gamble (SG), time trade-off (TTO), and visual analog scale (VAS), have not been assessed in this patient population. To assess the convergent and construct validities of 5 different HU derivation methods in patients with head and neck cancer. In a cross-sectional study, we recruited 100 consecutive patients with squamous cell carcinoma of the upper aerodigestive tract treated in the outpatient surgical oncology clinics of the Princess Margaret Cancer Centre from August 1 through October 31, 2014. We enrolled patients with a minimum of 3 months of follow-up after completion of treatment and no evidence of recurrent or metastatic disease. Par...
Head & neck, Jan 9, 2014
Background With new minimally invasive surgical techniques playing a role in the management of ea... more Background With new minimally invasive surgical techniques playing a role in the management of early oropharyngeal carcinomas, appropriate surgical management of the neck is an increasingly important consideration. Methods We present a case of a 47-year-old patient with a clinical T2N1M0 squamous cell carcinoma of the right tonsil with anterior extension towards the floor of mouth from the right glossotonsillar sulcus. Imaging revealed a solitary necrotic lymph node in IIA. The decision was made to proceed with a transoral robotic pharyngectomy and selective neck dissection (SND) of levels II-IV, followed by adjuvant radiotherapy. Results A post-operative scan revealed progressive regional disease in the anterior aspect of level IB, and, as such, a completion neck dissection of level I was performed. Conclusion Special consideration must be given to level I particularly in patients with glossotonsillar sulcus involvement and anterior extension towards the floor of mouth. This articl...
JAMA Ophthalmology, 2015
IMPORTANCE The ocular status of homeless populations remains largely unknown. Given that visual a... more IMPORTANCE The ocular status of homeless populations remains largely unknown. Given that visual acuity has been shown to be heavily correlated with reduced well-being and decreased earning potential, findings of poor vision could have important health implications for people experiencing homelessness.
Purpose: Biosynthetic corneal hydrogels have already undergone successful implantation in human p... more Purpose: Biosynthetic corneal hydrogels have already undergone successful implantation in human patients with keratoconus and may one day serve as useful alternatives to human donor tissue for surgical and refractive procedures. For synthetic materials to be biocompatible, they should undergo laser ablation in a consistent and reproducible manner. The purpose of our study was 1) to determine the feasibility and consistency of excimer ablation of collagen-based corneal biomimics (hydrogels), 2) to compare the ablation rates of hydrogels with different mechanical properties. Methods: Twelve samples of four different hydrogel formulations (10% recombinant porcine collagen) underwent identical excimer ablations (125 µm PTK, 6.0 mm). All hydrogels were cross-linked using a carbodiimide (EDC), epoxy-based cross-linker (BDDGE) or combination (hybrid) to enhance their mechanical properties. Hydrogel ablations were captured using a specialized high resolution camera (optical profilometer). C...
Journal of Functional Biomaterials, 2013
A bi-functional epoxy-based cross-linker, 1,4-Butanediol diglycidyl ether (BDDGE), was investigat... more A bi-functional epoxy-based cross-linker, 1,4-Butanediol diglycidyl ether (BDDGE), was investigated in the fabrication of collagen based corneal substitutes. Two synthetic strategies were explored in the preparation of the cross-linked collagen scaffolds. The lysine residues of Type 1 porcine collagen were directly cross-linked using OPEN ACCESS J. Funct. Biomater. 2013, 4 163
Journal of Functional Biomaterials, 2014
Journal of Nanoparticle Research, 2013
Thermally denatured human serum albumin interacts with *3.0 nm spherical AgNP enhancing the fluor... more Thermally denatured human serum albumin interacts with *3.0 nm spherical AgNP enhancing the fluorescence of Trp-214 at large protein/ nanoparticle ratios. However, using native HSA, no changes in the emission were observed. The observation is likely due to differences between native and denatured protein packing resulting from protein corona formation. We have also found that NH 2 blocking of the protein strongly affects the ability of the protein to protect AgNP from different salts/ions such as NaCl, PBS, Hank's buffer, Tris-HCl, MES, and DMEM. Additionally, AgNP can be readily prepared in aqueous solutions by a photochemical approach employing HSA as an in situ protecting agent. The role of the protein in this case is beyond that of protecting agent; thus, Ag ? ions and I-2959 complexation within the protein structure also affects the efficiency of AgNP formation. Blocking NH 2 in HSA modified the AgNP growth profile, surface plasmon band shape, and long-term stability suggesting that amine groups are directly involved in the formation and post-stabilization of AgNP. In particular, AgNP size and shape are extensively influenced by NH 2 blocking, leading primarily to cubes and plates with sizes around 5-15 nm; in contrast, spherical monodisperse 4.0 nm AgNP are observed for native HSA. The nanoparticles prepared by this protocol are non-toxic in primary cells and have remarkable antibacterial properties. Finally, surface plasmon excitation of native HSA@AgNP promoted loss of protein conformation in just 5 min, suggesting that plasmon heating causes protein denaturation using continuous light sources such as commercial LED.
Nanoscale, 2015
The increasing number of multidrug resistant bacteria has revitalized interest in seeking alterna... more The increasing number of multidrug resistant bacteria has revitalized interest in seeking alternative sources for controlling bacterial infection. Silver nanoparticles (AgNPs), are amongst the most promising candidates due to their wide microbial spectrum of action. In this work, we report on the safety and efficacy of the incorporation of collagen coated AgNPs into collagen hydrogels for tissue engineering. The resulting hybrid materials at [AgNPs] < 0.4 μM retained the mechanical properties and biocompatibility for primary human skin fibroblasts and keratinocytes of collagen hydrogels; they also displayed remarkable anti-infective properties against S. aureus, S. epidermidis, E. coli and P. aeruginosa at considerably lower concentrations than silver nitrate. Further, subcutaneous implants of materials containing 0.2 μM AgNPs in mice showed a reduction in the levels of IL-6 and other inflammation markers (CCL24, sTNFR-2, and TIMP1). Finally, an analysis of silver contents in implanted mice showed that silver accumulation primarily occurred within the tissue surrounding the implant.
articles by Christopher Noel
JAMA otolaryngology-- head & neck surgery, Jan 23, 2015
Accurate measurement of health state utilities (HU) is the cornerstone for cost-utility analyses ... more Accurate measurement of health state utilities (HU) is the cornerstone for cost-utility analyses and the valuation of quality of life for given health states. Current indirect methods of HU derivation lack face validity for patients with head and neck cancer. The appropriateness of these measures compared with direct methods, such as the standard gamble (SG), time trade-off (TTO), and visual analog scale (VAS), have not been assessed in this patient population. To assess the convergent and construct validities of 5 different HU derivation methods in patients with head and neck cancer. In a cross-sectional study, we recruited 100 consecutive patients with squamous cell carcinoma of the upper aerodigestive tract treated in the outpatient surgical oncology clinics of the Princess Margaret Cancer Centre from August 1 through October 31, 2014. We enrolled patients with a minimum of 3 months of follow-up after completion of treatment and no evidence of recurrent or metastatic disease. Par...
Head & neck, Jan 9, 2014
Background With new minimally invasive surgical techniques playing a role in the management of ea... more Background With new minimally invasive surgical techniques playing a role in the management of early oropharyngeal carcinomas, appropriate surgical management of the neck is an increasingly important consideration. Methods We present a case of a 47-year-old patient with a clinical T2N1M0 squamous cell carcinoma of the right tonsil with anterior extension towards the floor of mouth from the right glossotonsillar sulcus. Imaging revealed a solitary necrotic lymph node in IIA. The decision was made to proceed with a transoral robotic pharyngectomy and selective neck dissection (SND) of levels II-IV, followed by adjuvant radiotherapy. Results A post-operative scan revealed progressive regional disease in the anterior aspect of level IB, and, as such, a completion neck dissection of level I was performed. Conclusion Special consideration must be given to level I particularly in patients with glossotonsillar sulcus involvement and anterior extension towards the floor of mouth. This articl...
JAMA Ophthalmology, 2015
IMPORTANCE The ocular status of homeless populations remains largely unknown. Given that visual a... more IMPORTANCE The ocular status of homeless populations remains largely unknown. Given that visual acuity has been shown to be heavily correlated with reduced well-being and decreased earning potential, findings of poor vision could have important health implications for people experiencing homelessness.
Purpose: Biosynthetic corneal hydrogels have already undergone successful implantation in human p... more Purpose: Biosynthetic corneal hydrogels have already undergone successful implantation in human patients with keratoconus and may one day serve as useful alternatives to human donor tissue for surgical and refractive procedures. For synthetic materials to be biocompatible, they should undergo laser ablation in a consistent and reproducible manner. The purpose of our study was 1) to determine the feasibility and consistency of excimer ablation of collagen-based corneal biomimics (hydrogels), 2) to compare the ablation rates of hydrogels with different mechanical properties. Methods: Twelve samples of four different hydrogel formulations (10% recombinant porcine collagen) underwent identical excimer ablations (125 µm PTK, 6.0 mm). All hydrogels were cross-linked using a carbodiimide (EDC), epoxy-based cross-linker (BDDGE) or combination (hybrid) to enhance their mechanical properties. Hydrogel ablations were captured using a specialized high resolution camera (optical profilometer). C...
Journal of Functional Biomaterials, 2013
A bi-functional epoxy-based cross-linker, 1,4-Butanediol diglycidyl ether (BDDGE), was investigat... more A bi-functional epoxy-based cross-linker, 1,4-Butanediol diglycidyl ether (BDDGE), was investigated in the fabrication of collagen based corneal substitutes. Two synthetic strategies were explored in the preparation of the cross-linked collagen scaffolds. The lysine residues of Type 1 porcine collagen were directly cross-linked using OPEN ACCESS J. Funct. Biomater. 2013, 4 163
Journal of Functional Biomaterials, 2014
Journal of Nanoparticle Research, 2013
Thermally denatured human serum albumin interacts with *3.0 nm spherical AgNP enhancing the fluor... more Thermally denatured human serum albumin interacts with *3.0 nm spherical AgNP enhancing the fluorescence of Trp-214 at large protein/ nanoparticle ratios. However, using native HSA, no changes in the emission were observed. The observation is likely due to differences between native and denatured protein packing resulting from protein corona formation. We have also found that NH 2 blocking of the protein strongly affects the ability of the protein to protect AgNP from different salts/ions such as NaCl, PBS, Hank's buffer, Tris-HCl, MES, and DMEM. Additionally, AgNP can be readily prepared in aqueous solutions by a photochemical approach employing HSA as an in situ protecting agent. The role of the protein in this case is beyond that of protecting agent; thus, Ag ? ions and I-2959 complexation within the protein structure also affects the efficiency of AgNP formation. Blocking NH 2 in HSA modified the AgNP growth profile, surface plasmon band shape, and long-term stability suggesting that amine groups are directly involved in the formation and post-stabilization of AgNP. In particular, AgNP size and shape are extensively influenced by NH 2 blocking, leading primarily to cubes and plates with sizes around 5-15 nm; in contrast, spherical monodisperse 4.0 nm AgNP are observed for native HSA. The nanoparticles prepared by this protocol are non-toxic in primary cells and have remarkable antibacterial properties. Finally, surface plasmon excitation of native HSA@AgNP promoted loss of protein conformation in just 5 min, suggesting that plasmon heating causes protein denaturation using continuous light sources such as commercial LED.
Nanoscale, 2015
The increasing number of multidrug resistant bacteria has revitalized interest in seeking alterna... more The increasing number of multidrug resistant bacteria has revitalized interest in seeking alternative sources for controlling bacterial infection. Silver nanoparticles (AgNPs), are amongst the most promising candidates due to their wide microbial spectrum of action. In this work, we report on the safety and efficacy of the incorporation of collagen coated AgNPs into collagen hydrogels for tissue engineering. The resulting hybrid materials at [AgNPs] < 0.4 μM retained the mechanical properties and biocompatibility for primary human skin fibroblasts and keratinocytes of collagen hydrogels; they also displayed remarkable anti-infective properties against S. aureus, S. epidermidis, E. coli and P. aeruginosa at considerably lower concentrations than silver nitrate. Further, subcutaneous implants of materials containing 0.2 μM AgNPs in mice showed a reduction in the levels of IL-6 and other inflammation markers (CCL24, sTNFR-2, and TIMP1). Finally, an analysis of silver contents in implanted mice showed that silver accumulation primarily occurred within the tissue surrounding the implant.