Natalia Bailon-Moscoso | Universidad Tecnica Particular de Loja (original) (raw)
Papers by Natalia Bailon-Moscoso
Revista de Medicina y Cine / Journal of Medicine and Movies, Jun 15, 2023
Resumen Los largometrajes han sido utilizados como una herramienta de aprendizaje llamativa en el... more Resumen Los largometrajes han sido utilizados como una herramienta de aprendizaje llamativa en el campo de la salud, esto debido a que ciertas enfermedades que suelen ser raras dentro de la población se prestan para desarrollar una historia en base a las mismas. La Osteogénesis imperfecta conocida también como la enfermedad de los huesos de cristal, es un trastorno genético bastante diverso, si bien el 90 % de los casos se presentan con mutaciones de herencia de tipo autosómicas dominantes, un 10 % restante corresponde a mutaciones autosómicas recesivas o de causa desconocida. En el presente trabajo hemos generado una recopilación de aspectos relevantes sobre esta enfermedad correlacionando con la trama de dos trabajos cinematográficos, Frágiles y El protegido; esto con el fin de analizarlo como una posible herramienta para el aprendizaje de esta enfermedad y de otras patologías de carácter genético. Palabras claves: enfermedades genéticas; osteogénesis imperfecta; cinema-educación; colágeno; huesos.
Educación Médica, Jul 1, 2023
Revista de Medicina y Cine / Journal of Medicine and Movies, Jun 2, 2022
Resumen El cine es una efectiva herramienta de enseñanza-aprendizaje que permite ilustrar desde d... more Resumen El cine es una efectiva herramienta de enseñanza-aprendizaje que permite ilustrar desde diversos enfoques los procesos complejos de la enfermedad. En este artículo hacemos hincapié sobre estas herramientas educativas que nos brinda la película A dos metros de ti / Five feet apart, sobre la fibrosis quística. En la que se retratan los aspectos diarios que sobrellevan los pacientes dentro de un hospital desde su alimentación, tratamiento e infecciones. A la vez, la necesidad del contacto físico que tienen con las personas que conviven; ya que estos pacientes deben mantenerse alejados entre sí, a dos metros de distancia. Además, trata de las infecciones causadas por bacterias multirresistentes como Burkholderia cepacia y métodos alternativos como el proceso de trasplante pulmonar que permite prolongar la vida en paciente con fibrosis quística. Palabras clave: fibrosis quística; CFTR; Burkholderia cepacia; resistencia bacteriana; educación. Summary Movies are a helpful tool to learn and teach which allow us to depict from different points of view the complexity of the illness. In this paper we focus on the educational tools we saw in the movie Five feet apart about cystic fibrosis, where we can see the daily aspects that patients have
Molecules, Aug 17, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Journal of Pharmacognosy and Phytochemistry, 2015
Bejaria resinosa Mutis ex LF is also known as “pena de cerro” and is a plant species used traditi... more Bejaria resinosa Mutis ex LF is also known as “pena de cerro” and is a plant species used traditionally by the Saraguro ethnic group in Ecuador to treat nervous system problems, swollen wounds and inflammations of the genital organs, as well liver diseases and cancer. The aim of this study was to screen Bejaria resinosa aqueous extract and isolated compounds from organic extracts, for activity against the following cancer cell lines: MCF-7 (breast carcinoma), PC-3 (prostate carcinoma), RKO (colon carcinoma), and D-384 (astrocytoma). This was followed by identification of the compounds responsible for this effect. The results indicated that ursolic acid is the principle active responsible for the cytotoxicity on tumor cells.
SN Applied Sciences
Although the western discovery of borojó [Alibertia patinoiCuatrec. (Delprete & C.H.Perss.)] is a... more Although the western discovery of borojó [Alibertia patinoiCuatrec. (Delprete & C.H.Perss.)] is as recent as 1948, its several traditional uses in gastronomy and medicine, and its fame as an aphrodisiac are long standing and strong: the “love juice” extracted from it is very appreciated in Colombia, Ecuador, and Panama. Its medicinal potential, though, is far wider. This literature review aims to summarize the knowledge about the fruit, its ethnomedical uses, its biological activity and phytochemical composition, to validate ethnomedical claims and to help envision future lines of research. Borojó extracts have confirmed antimicrobial and antioxidant, and potential anticancer activities, which can be at least partially explained by its phytochemical composition -compounds isolated and identified through Gas Chromatography, High Performance Liquid Chromatography and spectroscopic and spectrometric techniques- rich in phenolic compounds, some of which, for example oleuropein, chloroge...
Plants
A pre-Columbian staple, Bactris gasipaes Kunth. is a palm tree domesticated around 4000 years ago... more A pre-Columbian staple, Bactris gasipaes Kunth. is a palm tree domesticated around 4000 years ago, so appreciated that a Spanish chronicler wrote in 1545, “only their wives and children were held in higher regard” by the Mesoamerican natives. The peach palm is an integral part of the foodways and gastronomy of Ecuador, Colombia, Bolivia, Peru, Brazil, and other tropical American countries; meanwhile, it is almost unknown in the rest of the world, except for hearts of palm. Although abundant, the species faces anthropogenic threats. The purpose of this study is to describe and summarize the physicochemical, nutritional, and bioactive characteristics of the peach palm and its two main alimentary products: hearts of palm and fruits, highlighting the functional and antioxidant potential of the latter, showing both ancestral and modern uses. There is active research on peach palm products and coproducts that aim for better, more sustainable uses of its traditional and recently found prop...
Environmental stress as a consequence of anthropic pressures can affect the physiological conditi... more Environmental stress as a consequence of anthropic pressures can affect the physiological condition of animals and cause damage to their DNA. Multiple studies have shown genotoxic effects of different pollutants; however, to our knowledge, the impact of environmental stress on genetic damage has been scarce explored. In some stressful ecosystems, such as seasonally dry tropical forests, the combined effects of anthropogenic activities and ongoing global changes cause increased environmental stresses that could trigger physiological and genetic effects on biodiversity. In this study, we evaluated the changes in the prevalence of genotoxic damage to birds in three states of forest degradation in the Tumbesian Region of Western Ecuador. We used the blood samples of 52 bird species to determine the frequency of micronucleus and nuclear abnormalities in the erythrocytes. Our results showed a significant effect of forest degradation in the proportion of micronucleus and nuclear abnormalit...
![Research paper thumbnail of Synthesis and evaluation of the biological activities of some 3-{[5-(6-methyl-4-aryl-2-oxo-1,2,3,4-tetrahydropyrimidin-5-yl)-1,3,4-oxadiazol-2-yl]-imino}-1,3-dihydro-2H-indol-2-one derivatives](https://attachments.academia-assets.com/107366394/thumbnails/1.jpg)
](Acta Pharmaceutica, 2008
Pyrimidines are well known for their anticancer, antimicrobial, antioxidant and antiviral activit... more Pyrimidines are well known for their anticancer, antimicrobial, antioxidant and antiviral activities (1-5). Oxadiazoles are well known for their antifungal, antibacterial, anticancer and anticonvulsant activity (6-10). Indoles are also well known compounds for their antimicrobial, anticancer, antioxidant and antiviral activity (11-15). In the present investigation nine pyrimidinone-oxadiazolyl indolinones were prepared. The synthesized indole derivatives of oxadiazolyl-pyrimidinones were tested for their antimicrobial and antioxidant activities.
Revista de Medicina y Cine / Journal of Medicine and Movies, Dec 20, 2022
Medicinal Chemistry Research
The unique etiology of cancer requires a multidimensional approach for its treatment, control, an... more The unique etiology of cancer requires a multidimensional approach for its treatment, control, and prevention. Therefore, all approaches to drug discovery and development should be exploited. Argentatin B (1) is a cycloartane triterpene isolated from Parthenium argentatum with inhibitory activity on tumor lines. The aim of this study is to investigate the inhibitory effect of 1 and 10 derivatives on the proliferation of a human colon cancer cell line (RKO), their genotoxic effects on human lymphocytes, as well as their potential effect on tumor protein (TP)-p53 and TP73 expression and phosphorylation. Argentatin B was found to induce reduced survival of RKO cells and showed a cytostatic effect. However, it did not induce apoptosis of RKO cells at the concentrations tested. Argentatin B and its derivatives were found to arrest the cell cycle at the G 1 phase. The bromine 2 and oxime 6 derivatives were more active than 1. Furthermore, 1 and its bromine (2) and oxime (6) derivatives induced phosphorylation of (TP)-p53 and TP73. Nevertheless, 2 exhibited a greater genotoxic effect on normal human lymphocytes than 6.
Reference Series in Phytochemistry, 2019
Toxicology Reports, 2022
Pollution from oil spills can seriously affect many ecosystem processes and human health. Many ar... more Pollution from oil spills can seriously affect many ecosystem processes and human health. Many articles have evaluated the impact of oil spills on human health. However, most of these articles focus on occupational exposure. The effect on people living in the areas affected by oil pollution is rarely studied. Approximately 640 million people worldwide live in areas at risk of oil pollution. Thus, studying the impact of this pollution on human health should be a priority. Here, we evaluate the presence of anemia in relation to crude oil exposure in men living in areas at risk of oil contamination in the Ecuadorian Amazon (Orellana and Napo). We evaluated the hematological and biochemical parameters of 135 participants. We divided the participants into three groups according to exposure: low, medium, and high. Our results showed a significant association between exposure risk and hemoglobin and hematocrit concentration. Groups with medium- and high- contamination exposure had levels below normal values in hemoglobin and hematocrit in more than 30% and 26% of the population, respectively. In conclusion, we found that crude oil affected human health, and the prevalence of the anemia in men was dependent of the level of contamination.
Revista de Medicina y Cine / Journal of Medicine and Movies, 2020
Revista de Medicina y Cine, 2022
Un enfoque cinematográfico sobre la realidad que viven las familias de los niños que padecen enfe... more Un enfoque cinematográfico sobre la realidad que viven las familias de los niños que padecen enfermedades raras, es lo que vemos en la película “Mi hija, mi vida” (Tu Vivras ma fille, 2018) de Gabriel Aghion. La primera hija de un matrimonio feliz nace su hija con el Síndrome de Sanfilippo, una patología de carácter genético poco frecuente donde existe un deterioro de las funcionalidades a partir de los 3 años y para la cual no existe un tratamiento efectivo. Está película, basada en hechos de la vida real, nos muestra el esfuerzo que algunas personas son capaces de hacer con la finalidad de encontrar nuevas opciones de tratamiento con la finalidad de mejorar la vida de los seres que amamos, y en esa búsqueda desarrollan un tratamiento basado en los últimos desarrollos de la biotecnología, la terapia génica, que logra la disminución de síntomas o conductas puntuales, propias de la patología. En este artículo hacemos un enfoque de la investigación para la búsqueda de un tratamiento c...
Additional file 1: Fig. S1. Original western blots CL-X Posure™ films for Western blots. Each pro... more Additional file 1: Fig. S1. Original western blots CL-X Posure™ films for Western blots. Each protein was detected in independent films at different times of exposure to the membrane. A) p53; B) p21; C) LC3-I and -II; D) p62; E) BAX; F) Bcl-2; G) Cleaved caspase-3; H) Active and cleaved PARP-1; I) Actin. Notice, for autophagy detection starvation control was first, in contrast to apoptosis control, which UV control was first. Fig. S2. 1H NMR spectrum of compound 3a. Fig. S3. 1H NMR spectrum of compound 3b. Fig. S4. 1H NMR spectrum of compound 3c. Fig. S5. 1H NMR spectrum of compound 3d. Fig. S6. 1H NMR spectrum of compound 3e. Fig. S7. 19F NMR spectrum of compound 2e. Fig. S8. 1H NMR spectrum of compound 3f. Fig. S9. 1H NMR spectrum of compound 3 g. Fig. S10. 13C NMR spectrum of compound 3 g. Fig. S11. FTIR spectrum of compound 3 g. Fig. S12. ESI–MS spectrum of compound 3 g. Fig. S13. 1H NMR spectrum of compound 3 h. Fig. S14. 1H NMR spectrum of compound 3i. Fig. S15. 13C NMR spectr...
Supporting Information. Synthesis and evaluation of biological activities of bis(spiropyrazolone)... more Supporting Information. Synthesis and evaluation of biological activities of bis(spiropyrazolone)cyclopropanes: a potential application against leishmaniasis
The number of colon cancer patients is increasing, and new alternatives for treatment are importa... more The number of colon cancer patients is increasing, and new alternatives for treatment are important. We focused on the sesquiterpene lactone onoseriolide from <i>Hedyosmum racemosum</i>, which is widely used in traditional medicine. This compound was evaluated to determine its cytotoxic effect and the mechanism of cell death that is induced in the human colon cancer cell line RKO. A dose-dependent decrease in cell viability was observed. p53 expression increased followed by an increase in p21 expression, which is involved in cell cycle arrest in the G<sub>2</sub>/M phase. Caspase-3 activation and PARP-1 cleavage, which are apoptotic markers, were also monitored. Autophagy markers were also studied, and Beclin 1 was downregulated, while LC-3II increased in a dose-dependent manner. There were no changes in SQSTM1/p62 regulation. Onoseriolide exerts cytotoxic and cytostatic effects, activating the autophagy pathway as a protective mechanism and apoptosis as the ...
Sustainability, 2021
Background: contamination of aquatic ecosystems by oil spills associated with petroleum exploitat... more Background: contamination of aquatic ecosystems by oil spills associated with petroleum exploitation represents a serious problem of environmental contamination that can affect human health. We developed a spatial model of contamination risk in the Ecuadorian Amazon, and evaluated the model using independent datasets on environmental contamination and clinical indicators of human health risk factors. Methods: the spatial risk of contamination for the Napo River basin was based on the calculation of a friction surface and the accessibility of possible oil contamination. Human health was evaluated using peripheral blood samples from 256 individuals. We used monitoring data on contamination to validate the spatial model of contamination risk and analyzed whether the estimated risk explained the incidence of human health risk factors. Results: our risk model showed a significant association with actual contamination detected in the study area. According to our risk model, around 30% of ...
espanolLa nanotecnologia es el estudio, diseno, creacion, sintesis, manipulacion y aplicacion de ... more espanolLa nanotecnologia es el estudio, diseno, creacion, sintesis, manipulacion y aplicacion de materiales a traves del control de la materia a escala nanometrica, entre 1 a 100 nanometros. Se considera que la nanoteconologia esta revolucionando nuestro mundo. Los nanomateriales son ampliamente utilizados en diferentes campos por sus propiedades unicas pero tambien pueden causar efectos adversos tanto para la salud humana como para el ambiente, por lo que es necesario conocer los riesgos y los factores que incrementan estos efectos adversos. En esta revision abordamos los nanomateriales y sus tipos, los aspectos toxicos con enfasis en el efecto genotoxico, ya que por sus implicaciones de danos en el ADN pueden iniciar y promover la carcinogenesis, o afectar la fertilidad, ciertas consideraciones que deben tomarse en cuenta en el momento de la evaluacion genotoxica de los NMs. Aunque existe cierta evidencia de alteraciones al exponer las celulas a los nanomateriales, todavia existen...
Revista de Medicina y Cine / Journal of Medicine and Movies, Jun 15, 2023
Resumen Los largometrajes han sido utilizados como una herramienta de aprendizaje llamativa en el... more Resumen Los largometrajes han sido utilizados como una herramienta de aprendizaje llamativa en el campo de la salud, esto debido a que ciertas enfermedades que suelen ser raras dentro de la población se prestan para desarrollar una historia en base a las mismas. La Osteogénesis imperfecta conocida también como la enfermedad de los huesos de cristal, es un trastorno genético bastante diverso, si bien el 90 % de los casos se presentan con mutaciones de herencia de tipo autosómicas dominantes, un 10 % restante corresponde a mutaciones autosómicas recesivas o de causa desconocida. En el presente trabajo hemos generado una recopilación de aspectos relevantes sobre esta enfermedad correlacionando con la trama de dos trabajos cinematográficos, Frágiles y El protegido; esto con el fin de analizarlo como una posible herramienta para el aprendizaje de esta enfermedad y de otras patologías de carácter genético. Palabras claves: enfermedades genéticas; osteogénesis imperfecta; cinema-educación; colágeno; huesos.
Educación Médica, Jul 1, 2023
Revista de Medicina y Cine / Journal of Medicine and Movies, Jun 2, 2022
Resumen El cine es una efectiva herramienta de enseñanza-aprendizaje que permite ilustrar desde d... more Resumen El cine es una efectiva herramienta de enseñanza-aprendizaje que permite ilustrar desde diversos enfoques los procesos complejos de la enfermedad. En este artículo hacemos hincapié sobre estas herramientas educativas que nos brinda la película A dos metros de ti / Five feet apart, sobre la fibrosis quística. En la que se retratan los aspectos diarios que sobrellevan los pacientes dentro de un hospital desde su alimentación, tratamiento e infecciones. A la vez, la necesidad del contacto físico que tienen con las personas que conviven; ya que estos pacientes deben mantenerse alejados entre sí, a dos metros de distancia. Además, trata de las infecciones causadas por bacterias multirresistentes como Burkholderia cepacia y métodos alternativos como el proceso de trasplante pulmonar que permite prolongar la vida en paciente con fibrosis quística. Palabras clave: fibrosis quística; CFTR; Burkholderia cepacia; resistencia bacteriana; educación. Summary Movies are a helpful tool to learn and teach which allow us to depict from different points of view the complexity of the illness. In this paper we focus on the educational tools we saw in the movie Five feet apart about cystic fibrosis, where we can see the daily aspects that patients have
Molecules, Aug 17, 2021
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Journal of Pharmacognosy and Phytochemistry, 2015
Bejaria resinosa Mutis ex LF is also known as “pena de cerro” and is a plant species used traditi... more Bejaria resinosa Mutis ex LF is also known as “pena de cerro” and is a plant species used traditionally by the Saraguro ethnic group in Ecuador to treat nervous system problems, swollen wounds and inflammations of the genital organs, as well liver diseases and cancer. The aim of this study was to screen Bejaria resinosa aqueous extract and isolated compounds from organic extracts, for activity against the following cancer cell lines: MCF-7 (breast carcinoma), PC-3 (prostate carcinoma), RKO (colon carcinoma), and D-384 (astrocytoma). This was followed by identification of the compounds responsible for this effect. The results indicated that ursolic acid is the principle active responsible for the cytotoxicity on tumor cells.
SN Applied Sciences
Although the western discovery of borojó [Alibertia patinoiCuatrec. (Delprete & C.H.Perss.)] is a... more Although the western discovery of borojó [Alibertia patinoiCuatrec. (Delprete & C.H.Perss.)] is as recent as 1948, its several traditional uses in gastronomy and medicine, and its fame as an aphrodisiac are long standing and strong: the “love juice” extracted from it is very appreciated in Colombia, Ecuador, and Panama. Its medicinal potential, though, is far wider. This literature review aims to summarize the knowledge about the fruit, its ethnomedical uses, its biological activity and phytochemical composition, to validate ethnomedical claims and to help envision future lines of research. Borojó extracts have confirmed antimicrobial and antioxidant, and potential anticancer activities, which can be at least partially explained by its phytochemical composition -compounds isolated and identified through Gas Chromatography, High Performance Liquid Chromatography and spectroscopic and spectrometric techniques- rich in phenolic compounds, some of which, for example oleuropein, chloroge...
Plants
A pre-Columbian staple, Bactris gasipaes Kunth. is a palm tree domesticated around 4000 years ago... more A pre-Columbian staple, Bactris gasipaes Kunth. is a palm tree domesticated around 4000 years ago, so appreciated that a Spanish chronicler wrote in 1545, “only their wives and children were held in higher regard” by the Mesoamerican natives. The peach palm is an integral part of the foodways and gastronomy of Ecuador, Colombia, Bolivia, Peru, Brazil, and other tropical American countries; meanwhile, it is almost unknown in the rest of the world, except for hearts of palm. Although abundant, the species faces anthropogenic threats. The purpose of this study is to describe and summarize the physicochemical, nutritional, and bioactive characteristics of the peach palm and its two main alimentary products: hearts of palm and fruits, highlighting the functional and antioxidant potential of the latter, showing both ancestral and modern uses. There is active research on peach palm products and coproducts that aim for better, more sustainable uses of its traditional and recently found prop...
Environmental stress as a consequence of anthropic pressures can affect the physiological conditi... more Environmental stress as a consequence of anthropic pressures can affect the physiological condition of animals and cause damage to their DNA. Multiple studies have shown genotoxic effects of different pollutants; however, to our knowledge, the impact of environmental stress on genetic damage has been scarce explored. In some stressful ecosystems, such as seasonally dry tropical forests, the combined effects of anthropogenic activities and ongoing global changes cause increased environmental stresses that could trigger physiological and genetic effects on biodiversity. In this study, we evaluated the changes in the prevalence of genotoxic damage to birds in three states of forest degradation in the Tumbesian Region of Western Ecuador. We used the blood samples of 52 bird species to determine the frequency of micronucleus and nuclear abnormalities in the erythrocytes. Our results showed a significant effect of forest degradation in the proportion of micronucleus and nuclear abnormalit...
Acta Pharmaceutica, 2008
Pyrimidines are well known for their anticancer, antimicrobial, antioxidant and antiviral activit... more Pyrimidines are well known for their anticancer, antimicrobial, antioxidant and antiviral activities (1-5). Oxadiazoles are well known for their antifungal, antibacterial, anticancer and anticonvulsant activity (6-10). Indoles are also well known compounds for their antimicrobial, anticancer, antioxidant and antiviral activity (11-15). In the present investigation nine pyrimidinone-oxadiazolyl indolinones were prepared. The synthesized indole derivatives of oxadiazolyl-pyrimidinones were tested for their antimicrobial and antioxidant activities.
Revista de Medicina y Cine / Journal of Medicine and Movies, Dec 20, 2022
Medicinal Chemistry Research
The unique etiology of cancer requires a multidimensional approach for its treatment, control, an... more The unique etiology of cancer requires a multidimensional approach for its treatment, control, and prevention. Therefore, all approaches to drug discovery and development should be exploited. Argentatin B (1) is a cycloartane triterpene isolated from Parthenium argentatum with inhibitory activity on tumor lines. The aim of this study is to investigate the inhibitory effect of 1 and 10 derivatives on the proliferation of a human colon cancer cell line (RKO), their genotoxic effects on human lymphocytes, as well as their potential effect on tumor protein (TP)-p53 and TP73 expression and phosphorylation. Argentatin B was found to induce reduced survival of RKO cells and showed a cytostatic effect. However, it did not induce apoptosis of RKO cells at the concentrations tested. Argentatin B and its derivatives were found to arrest the cell cycle at the G 1 phase. The bromine 2 and oxime 6 derivatives were more active than 1. Furthermore, 1 and its bromine (2) and oxime (6) derivatives induced phosphorylation of (TP)-p53 and TP73. Nevertheless, 2 exhibited a greater genotoxic effect on normal human lymphocytes than 6.
Reference Series in Phytochemistry, 2019
Toxicology Reports, 2022
Pollution from oil spills can seriously affect many ecosystem processes and human health. Many ar... more Pollution from oil spills can seriously affect many ecosystem processes and human health. Many articles have evaluated the impact of oil spills on human health. However, most of these articles focus on occupational exposure. The effect on people living in the areas affected by oil pollution is rarely studied. Approximately 640 million people worldwide live in areas at risk of oil pollution. Thus, studying the impact of this pollution on human health should be a priority. Here, we evaluate the presence of anemia in relation to crude oil exposure in men living in areas at risk of oil contamination in the Ecuadorian Amazon (Orellana and Napo). We evaluated the hematological and biochemical parameters of 135 participants. We divided the participants into three groups according to exposure: low, medium, and high. Our results showed a significant association between exposure risk and hemoglobin and hematocrit concentration. Groups with medium- and high- contamination exposure had levels below normal values in hemoglobin and hematocrit in more than 30% and 26% of the population, respectively. In conclusion, we found that crude oil affected human health, and the prevalence of the anemia in men was dependent of the level of contamination.
Revista de Medicina y Cine / Journal of Medicine and Movies, 2020
Revista de Medicina y Cine, 2022
Un enfoque cinematográfico sobre la realidad que viven las familias de los niños que padecen enfe... more Un enfoque cinematográfico sobre la realidad que viven las familias de los niños que padecen enfermedades raras, es lo que vemos en la película “Mi hija, mi vida” (Tu Vivras ma fille, 2018) de Gabriel Aghion. La primera hija de un matrimonio feliz nace su hija con el Síndrome de Sanfilippo, una patología de carácter genético poco frecuente donde existe un deterioro de las funcionalidades a partir de los 3 años y para la cual no existe un tratamiento efectivo. Está película, basada en hechos de la vida real, nos muestra el esfuerzo que algunas personas son capaces de hacer con la finalidad de encontrar nuevas opciones de tratamiento con la finalidad de mejorar la vida de los seres que amamos, y en esa búsqueda desarrollan un tratamiento basado en los últimos desarrollos de la biotecnología, la terapia génica, que logra la disminución de síntomas o conductas puntuales, propias de la patología. En este artículo hacemos un enfoque de la investigación para la búsqueda de un tratamiento c...
Additional file 1: Fig. S1. Original western blots CL-X Posure™ films for Western blots. Each pro... more Additional file 1: Fig. S1. Original western blots CL-X Posure™ films for Western blots. Each protein was detected in independent films at different times of exposure to the membrane. A) p53; B) p21; C) LC3-I and -II; D) p62; E) BAX; F) Bcl-2; G) Cleaved caspase-3; H) Active and cleaved PARP-1; I) Actin. Notice, for autophagy detection starvation control was first, in contrast to apoptosis control, which UV control was first. Fig. S2. 1H NMR spectrum of compound 3a. Fig. S3. 1H NMR spectrum of compound 3b. Fig. S4. 1H NMR spectrum of compound 3c. Fig. S5. 1H NMR spectrum of compound 3d. Fig. S6. 1H NMR spectrum of compound 3e. Fig. S7. 19F NMR spectrum of compound 2e. Fig. S8. 1H NMR spectrum of compound 3f. Fig. S9. 1H NMR spectrum of compound 3 g. Fig. S10. 13C NMR spectrum of compound 3 g. Fig. S11. FTIR spectrum of compound 3 g. Fig. S12. ESI–MS spectrum of compound 3 g. Fig. S13. 1H NMR spectrum of compound 3 h. Fig. S14. 1H NMR spectrum of compound 3i. Fig. S15. 13C NMR spectr...
Supporting Information. Synthesis and evaluation of biological activities of bis(spiropyrazolone)... more Supporting Information. Synthesis and evaluation of biological activities of bis(spiropyrazolone)cyclopropanes: a potential application against leishmaniasis
The number of colon cancer patients is increasing, and new alternatives for treatment are importa... more The number of colon cancer patients is increasing, and new alternatives for treatment are important. We focused on the sesquiterpene lactone onoseriolide from <i>Hedyosmum racemosum</i>, which is widely used in traditional medicine. This compound was evaluated to determine its cytotoxic effect and the mechanism of cell death that is induced in the human colon cancer cell line RKO. A dose-dependent decrease in cell viability was observed. p53 expression increased followed by an increase in p21 expression, which is involved in cell cycle arrest in the G<sub>2</sub>/M phase. Caspase-3 activation and PARP-1 cleavage, which are apoptotic markers, were also monitored. Autophagy markers were also studied, and Beclin 1 was downregulated, while LC-3II increased in a dose-dependent manner. There were no changes in SQSTM1/p62 regulation. Onoseriolide exerts cytotoxic and cytostatic effects, activating the autophagy pathway as a protective mechanism and apoptosis as the ...
Sustainability, 2021
Background: contamination of aquatic ecosystems by oil spills associated with petroleum exploitat... more Background: contamination of aquatic ecosystems by oil spills associated with petroleum exploitation represents a serious problem of environmental contamination that can affect human health. We developed a spatial model of contamination risk in the Ecuadorian Amazon, and evaluated the model using independent datasets on environmental contamination and clinical indicators of human health risk factors. Methods: the spatial risk of contamination for the Napo River basin was based on the calculation of a friction surface and the accessibility of possible oil contamination. Human health was evaluated using peripheral blood samples from 256 individuals. We used monitoring data on contamination to validate the spatial model of contamination risk and analyzed whether the estimated risk explained the incidence of human health risk factors. Results: our risk model showed a significant association with actual contamination detected in the study area. According to our risk model, around 30% of ...
espanolLa nanotecnologia es el estudio, diseno, creacion, sintesis, manipulacion y aplicacion de ... more espanolLa nanotecnologia es el estudio, diseno, creacion, sintesis, manipulacion y aplicacion de materiales a traves del control de la materia a escala nanometrica, entre 1 a 100 nanometros. Se considera que la nanoteconologia esta revolucionando nuestro mundo. Los nanomateriales son ampliamente utilizados en diferentes campos por sus propiedades unicas pero tambien pueden causar efectos adversos tanto para la salud humana como para el ambiente, por lo que es necesario conocer los riesgos y los factores que incrementan estos efectos adversos. En esta revision abordamos los nanomateriales y sus tipos, los aspectos toxicos con enfasis en el efecto genotoxico, ya que por sus implicaciones de danos en el ADN pueden iniciar y promover la carcinogenesis, o afectar la fertilidad, ciertas consideraciones que deben tomarse en cuenta en el momento de la evaluacion genotoxica de los NMs. Aunque existe cierta evidencia de alteraciones al exponer las celulas a los nanomateriales, todavia existen...