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Papers by jack kettman

Molecular Immunology, 1997

A cDNA library was divided into 291 sectors of low complexity, 800-1000 recombinant phage plaques... more A cDNA library was divided into 291 sectors of low complexity, 800-1000 recombinant phage plaques per sector. Aliquots of DNA from sectors prepared from high titer phage were subjected to in vitro transcription using T7 polymerase and thereafter RNA was translated in a cellfree rabbit reticulocyte system in the presence of [35S] methionine. The resulting polypeptides were separated by 2D gel electrophoresis. Radiofluorographs of the gels prepared from 10 sectors were subjected to scanning and image analysis. A multilevel spot matching procedure was employed allowing us to recognise spot occurrence in the 10 sectors. The number of detected polypeptide spots per sector varied between 147 and 325. Overall, 1082 different spots were counted totalling 1983 spots considering multiple entries. The distribution of the spots and the frequency distribution of the RNA population among the 10 sectors are shown. The highest detected frequency is 10 2, while one half of the RNA molecular species are present at a frequency of 10-3 or lower.

Immunopharmacology, 1979

Mice receiving antigen (SRBC: sheep red blood cells) and vinblastine on the same day (or the day ... more Mice receiving antigen (SRBC: sheep red blood cells) and vinblastine on the same day (or the day after antigen stimulation) show greatly decreased numbers of plaque-forming ceils in the spleen on the fourth day after antigenic stimulation. There is also some loss of viable splenic lymphocytes by the fourth day after immunization and vinbla~ne administration. The apparent delayed-type footpad swelling responses of such vinblasttne-and antigen-treated mice are similar or greater than responses found in mice receiving antigen alone. The spleen ceils of antigen-and "vinbla~ne plus antigen"-treated mice show the same amount of helper cell activity. However, mice receiving vinblastine 2 days after antigen, but 3 days prior to delayed-type hypersensitivity (DTH) testing, show diminished DTH responses compared to mice receiving antigen alone. There is aLso a loss of peripheral blood leukocytes in such mice and this vinblastine-induced depression of the DTH response probably represents some loss of effector abilities of treated mice. The interpretation of the DTH reactivity is difficult, because mice receiving vinblastine alone demonstrated an anomalous footpad swelling reaction of broad specificity that is not associated with the development of either antibody-forming cells or helper activity in the spleen of treated mice. The nature of this reaction appeared to be largely edematous and its origin at this time is not completely understood. Such reactions show no apparent infiltration of mononuclear cells, in contrast to the responses of mice immunized with sheep red blood celLs or sheep red blood cells plus vinblastine. Because this anomalous reaction interferes with the quantitation of DTH reactions, it is difficult to draw conclusions concerning the effect of vinb]a~ne on the activation of cells that mediate DTH

Immunopharmacology, 1978

The chronic administration of Tilorone [(2,7-bis(diethylamino)ethoxy]fluoren-9-one) with antigen ... more The chronic administration of Tilorone [(2,7-bis(diethylamino)ethoxy]fluoren-9-one) with antigen seems to prevent the generation of DTH effector cells. The administration of Tilorone proximal to DTH challenge blocks the expression of DTH. By local transfer of DTH effector cells, this latter effect can be shown to be due to the lack of nonspecific inflammatory cells. By the use of both systemic and local transfer systems, it is also shown that specific DTH effector cells from animals treated with Tilorone are unable to circulate in the normal manner. Overall, these experiments suggest that Tilorone does not block the generation of DTH effector cells, but it does greatly alter the expression of DTH. The relation between these effects and the known interferon induction capacity on Tilorone remains unclear.

Methods for diagnosing syphilis

ACCESSORY CELL RESTRICTIONS ON IN VITRO GROWTH OF A T CELL LYMPHOMA: RESIDUAL ANTIGEN RECOGNITION RELATED TO THE LYMPHOMAGENIC PROCESS ?11This work was supported by NIH Grant AI-11630-08. D.L.J. was supported by NCI Training Grant CA-09082

B and T Cell Tumors, 1982

ABSTRACT A T cell lymphoma was found in an adult B10.S mouse which received (B10.S × 9R)F 1 lymph... more ABSTRACT A T cell lymphoma was found in an adult B10.S mouse which received (B10.S × 9R)F 1 lymphoid cells at birth in an attempt to induce tolerance across a portion of the MHC. Cell lines have been derived from the original tumor. One line requires TCGF for continuous growth and is tumerogenic. The cell surface phenotype of the cloned cell line is Thy 1.2 + , Ly.1 + , Ly.2 − . Growth restriction is demonstrated when the TCGF dependent tumor cells are plated at low cell density in Terasaki trays. Growth is dependent on adherant cells and the cloning efficiency of the tumor cells depends on the MHC of the adherent cell. In vivo tumor growth shows an identical pattern to the clonal growth in vitro .

Radioresistance of cells responsible for delayed hypersensitivity reactions in the mouse

Journal of immunology (Baltimore, Md. : 1950), 1975

The cells responsible for delayed hypersensitivity reactivity in the mouse act in a radioresistan... more The cells responsible for delayed hypersensitivity reactivity in the mouse act in a radioresistant fashion only if such irradiated cells are injected directly into the site of antigen challenge. Intravenous transfer of irradiated, primed spleen cells does not achieve a transfer of sensitivity to show delayed type hypersensitivity reactions. Transfer of sensitivity by the intravenous route can be effected by injection of non-irradiated spleen cells from primed mice.

Immunology Today, 1982

The ind~lction and regTdalion o/ irrzmune re~])m~se~ require the participation of cells which mai... more The ind~lction and regTdalion o/ irrzmune re~])m~se~ require the participation of cells which maintain lymphocyte viability, promote ~ecesvary celhdar interaclio~s, secrete imm~moreguIatory molecules arid presen! antzgen. These are accesso O, eeltr, and their ,fimctio,~ i,r r~ot antigen-specific. Classical{y, celtr qf the monomtclear phagocyte lineage kae:e beer~ eon.ridered to be the major accessory ceil po/mlation in most z'mm~me re.~])on.res. More receT~t{y, olker cell t~/)e.r, .v~ek as Langerhans cells of the .rkin and &ndritie cells of Ike lyr~pkoid orgar~.r, have also been shown Io be e[/}'cti~:e. This argiele reviews the evidence that additional cell type.r, such a.r endothelial cells or.fibroblasts, serve an aecesso(y role in immune re.~;tm~lses.

Proceedings of the National Academy of Sciences, 1974

The mode of action of “allogeneic supernatant” (the culture supernatant of a 24-hr mixedlymphocyt... more The mode of action of “allogeneic supernatant” (the culture supernatant of a 24-hr mixedlymphocyte reaction), has been studied. This factor stimulates the response of spleen cell cultures depleted in thymus-derived lymphoid cells (T-cells) to antigens that elicit a thymus-dependent response. We used a limiting dilution analysis, in which the frequency and size of response of individual bone-marrow-derived lymphoid cells (B-cells) could be measured. In confirmation of other reports, the occurrence of B-cells responding to antigen under different conditions was shown to follow a Poisson distribution in mouse spleen cell suspensions. Allogeneic supernatant increased responses to thymus-dependent antigens, both by increasing the frequency of B-cells whose response is initiated and by increasing the numbers of antibody-forming cells obtained from each responding B-cell. Two fractions were obtained by dialysis of the supernatant. The nondialyzable fraction contained factors able to increa...

The structure of the mouse immunoglobulin in γ 3 membrane gene segment

Nucleic Acids Research, 1983

... 11 x 45 6TG piasmacytoma line (received from MD Scharff, Albert Einstein, Bronx, New York) an... more ... 11 x 45 6TG piasmacytoma line (received from MD Scharff, Albert Einstein, Bronx, New York) and immune A/J spleen cells which had been stimulated in vitro by dextran sulfate (DxS) and Upopolysaccharide (LPS) as described by Robertson et a ... 6. Nevins, JR and Darnell, JE, Jr ...

Molecular Immunology, 1968

The conditions required for the formation of precipitin between gulnea-pig collagen and rabbit an... more The conditions required for the formation of precipitin between gulnea-pig collagen and rabbit antibodies were investigated. The amount ofprecipitin was found to be inversely related to the ionic strength of the solution and, to a lesser extent, inversely related to temperature. Gelatin, the heat-denatured form of collagen, could also form precipitins with anti-collagen, suggesting that the quaternary and tertiary structures of collagen played little or no role in the recognition and binding with the anti-collagen sera used.

Journal of Immunological Methods, 1978

Spleen cells of the primed guinea pig, when cultured alone, failed to make a primary immune respo... more Spleen cells of the primed guinea pig, when cultured alone, failed to make a primary immune response against heterologous erythrocytes. When supplemented with lymphocytes obtained from mineral oil induced peritoneal exudate (PEL), a primary immune response against sheep, horse and chicken RBC was obtained. The kinetics, dose response and requirement of PELs for this response are reported.

Cellular Immunology, 1979

Mice immunized with glutaraldehyde-fixed sheep red blood cells (G-SRBC) show delayed-type hyperse... more Mice immunized with glutaraldehyde-fixed sheep red blood cells (G-SRBC) show delayed-type hypersensitivity (DTH) reactions to G-SRBC or SRBC. The specificity of the DTH reaction of mice sensitized with glutaraldehyde-fixed antigens is similar to that found after sensitization with unfixed antigens. The dose-response curve for sensitization by glutaraldehyde-fixed SRBC was very different from the curve for normal SRBC. At low doses, both antigens were effective in sensitizing to show DTH but neither induced an antibody response. However, at high antigen doses, only the glutaraldehyde-fixed antigen was efficient in sensitizing to show DTH and it failed to raise an antibody titer. Spleen cells of mice sensitized with fixed RBC can transfer DTH locally but if the donor cells are irradiated (SO0 R), the transfer is abrogated. In contrast, the transfer of DTH by spleen cells of mice immunized with unfixed antigen is not affected by 500 R. The transfer of DTH by spleen cells of mice immunized with fixed antigen can be blocked by "in vitro desensitization" while the transfer of DTH by spleen cells from mice primed with normal antigen is resistant to "in vitro desensitization." These results suggest that immunization of mice with different physical states of the same antigen can result in the activation of antigen-specific T cells which exhibit markedly different properties.

Cellular Immunology, 1986

Adherent peritoneal cells (APC) have often been used as a pure and effective macrophage populatio... more Adherent peritoneal cells (APC) have often been used as a pure and effective macrophage population. Using partition analysis and small numbers of lymphoid cells activated by mitogens (concanavalin A for T cells (in the presence of TCGF) and LPS + DxS for B cells) we found that APC were accessory cells for T cell activation and growth but were not effective for B cells. Although APC were effective in assisting T-cell mitogenesis, they were not especially efficient. However, when APC were mixed with irradiated WEHI-cells (a tissue culture line previously shown to exhibit accessory cell activity in vitro for mitogenic activation T and B cells), the APC and WEHI-showed apparent synergy. One reason for failure of APC to assist B-cell mitogenesis was traced to the presence of a suppressor cell population which overcame the accessory cell help given by irradiated WEHI-cells to LPS-DxS stimulated murine B cells. It is thus possible to find "helper" effects (synergy of APC and WEHI-assisting the mitogenesis of T cells), as well as suppressor effects within the range of cells found in adherent accessory cells. o 1986 Academic Res, Inc.

Biochemical and Biophysical Research Communications, 1965

Previously we (Kettmsn and King, 1963) d-n&rated an oligomycinsensitive ATPase in the Keilin-Hsrt... more Previously we (Kettmsn and King, 1963) d-n&rated an oligomycinsensitive ATPase in the Keilin-Hsrtree heart muscle preparation (also g. HWi43 and Slater, 1961). The ATPase was also solubilized and the soluble enzyme wss insensitive to the antibiotic. This note reports some energy linked reactions in the Keilin-Hsrtree preparation. A soluble extract from heart mitochondria stimulates not only the transhydrogensse reaction but also phosphorylation when the heart muscle preparation is used as the electron transport system for succinate oxidation.

Cellular Immunology, 1982

Single murine B cells can be induced to grow clonally by a combination of mitogens (dextran sulfa... more Single murine B cells can be induced to grow clonally by a combination of mitogens (dextran sulfate and LPS) and the presence of appropriate accessory cells. We show here that irradiated cells of a tissue culture cell line (WEHI-3) can substitute for naturally occurring adherent cells. Single-cell growth as well as Ig secretion are induced by irradiated cells of WEHI-3. There are no histocompatibility restrictions between the cells of the accessory cell line and the responding B cells. Under limiting dilution conditions, dextran sulfate and LPS act synergistically in inducing both growth and Ig secretion in these WEHI-3-dependent cultures. Although Ig synthesis occurs only in cultures where B-cell growth occurs, not all clones of B cells produce Ig. This is apparently not related to the clone size or to the number of initiated precursor cells; instead mitogen-induced growing B-cell clones seem to be heterogeneous with respect to Ig production.

Radioresistance oftheEnhancing Effect ofCells fromCarrier-Immunized Mice inanInVitro PrimaryImmuneResponse

The invitroprimaryresponse ofmouse spleencellsuspensions to 2,4,6-trinitrophenyl(Tnp)- erythrocyt... more The invitroprimaryresponse ofmouse spleencellsuspensions to 2,4,6-trinitrophenyl(Tnp)- erythrocytes hasbeenstudied. Thenumberofanti-Tnp plaque-forming cells thatarise after antigenic stimulation invitro isgreatly enhancedbyprior immunization invivo withthecarrier erythrocyte. Theenhancement isantigen specific. The primingforan enhancedresponse canbe elicited withverylowantigen dosesandisoftenapparent 24hrafter immunization. Itismarkedfromday3today 14.Spleen cells fromcarrier-primed micewillenhancethe anti-Tnp response ofnormalcells whenmixedcultures of thetwocell populations arechallenged withTnp-erythro- cytesinvitro. The carrier-primed cells mediating this enhancing effect arethymusderived. The developmentof the thymus-derived, carrier- specific cellpopulation hasbeengenerally assumedto involve theantigenic stimulation ofcellproliferation. It was,therefore, somewhatsurprising to findthatthe

AnApproach to Molecular Analysis ofT-CellClonesbyTwo-Dimensional GelElectrophoresis: IsThereInterclonal andlntraclonal Heterogeneity?

Hybrid cell lines producing monoclonal antibodies directed against treponema and use thereof for immuno-diagnosis of treponemal infections

Using monoclonal antibodies specific for treponema bacterial pathogens

Hybrid cell lines producing monoclonal antibodies directed against Haemophilus influenzae