Dr. Aneela Z Durrani | University Of Veterinary And Animal Sciences Lahore (original) (raw)

Papers by Dr. Aneela Z Durrani

Animal Production Science, Jul 22, 2021

Context Breeds of buffalo (Nili-Ravi and Kundhi) show different growth-rate and meat characterist... more Context Breeds of buffalo (Nili-Ravi and Kundhi) show different growth-rate and meat characteristics. Consumer preferences vary for the selection of meat according to the taste and sensory attributes. Buffalo meat is widely consumed in Asia and multiple buffalo breeds are reared for meat production. Aims The current experiment was designed to compare growth rates, meat characteristics and consumer preference for meat from bulls of different buffalo breeds. Methods Thirty-five male Nili-Ravi buffalo bulls (NRBB) and 35 male Kundhi buffalo bulls (KBB) having an age of 18 ± 1.56 months and 18 ± 1.65 months with live bodyweight of 150 ± 1.98 kg and 149 ± 2.09 kg respectively, were purchased and reared for 90 days. All animals were slaughtered on the 91st day and samples from M. pectoralis descendance, M. latissimus dorsi and M. obliquus externus abdominis were collected. A consumer preference survey was also conducted to assess the preferences for meat from the buffalo breeds. Key results Results of the survey suggested that for buyers, meat colour was the primary selection criteria (P < 0.05) at the time of purchase. Consumers preferred the lighter-coloured meat of KBB (P < 0.05) than that of NRBB. Conclusions Findings of the study showed that meat from KBB had superior sensory attributes compared with NRBB, although the meat from NRBB was the more nutritious of the two buffalo breeds. Implications The results will provide a focussed view regarding selection of buffalo bulls for meat production.

Pakistan Journal of Zoology, 2013

Current study was conducted to evaluate the effect of two commercially available immunostimulants... more Current study was conducted to evaluate the effect of two commercially available immunostimulants Livol (herbal supplement) and Immunotone (selenium and vit. E) on growth performance and humoral response against IBD virus vaccine. In the present study 150 broiler chicks were purchased and divided into five groups namely A, B, C, D and E (30 chicks in each group). The birds of groups A, B and E were vaccinated with infectious bursal diseases (IBD) virus vaccine (Busaplex) on 1 st day of age. Birds of groups A and C were supplemented with Immunotone while group B and D were supplemented with Livol. The effect of immunostimulants on humoral immune response was evaluated by recording weekly serum antibody titers against IBD through indirect haemagglutination (IHA) test. No significant difference was found in the body weight of broiler chicks on 7, 14, 21 and 28 days of age among the different treatment groups but the body weight of groups B and D receiving Livol was significantly increased (P<0.05) after 35 days. No significant difference was found in the feed conversion ratio (FCR) during treatment period but on day 35 Livol treated groups B and D showed significantly higher FCR 1.52±0.02 and 1.48±0.02, respectively. The cumulative mean titers for IHA for different treatment groups A, B, C, D and E were 43.3, 51.4, 12.3, 14.1 and 40.1, respectively (P<0.05). These results indicate that Livol supplementation may help to increase post vaccination humoral immune response against IBD in broiler chicks.

IntechOpen eBooks, Feb 23, 2022

The main objective of this study is to investigate the presence of Aflatoxin M1 (AFM1) in local a... more The main objective of this study is to investigate the presence of Aflatoxin M1 (AFM1) in local and processed milk and main dairy products available in Lahore. Total 60 milk samples and 120 samples of dairy products including butter (n = 30), cheese (n = 30), cream (n = 30), and yogurt (n = 30) were collected. Milk samples were collected from 3 different sources i.e. unprocessed milk from local milk shop (n = 20) and a local dairy farm (n = 20), and processed milk sample from a commercial shop (n = 20) while samples of each dairy product were also different i.e. processed (n = 15) and unprocessed (n = 15). Milk samples were analyzed using kit method while dairy product samples were analyzed by high performance liquid chromatography (HPLC) technique equipped with fluorescence detector (HPLC-FLD) followed by immunoaffinity column clean up. In second phase of the study, efficacy of three different toxin binders was compared and analyzed. The results showed that AFM1 was detected in 16.7% of processed butter samples, 33.3% of processed cheese samples, 13.3% of local cream samples and 26.6% of processed yogurt samples and these samples exceeds European Union (EU) permissible limits of 0.05 ppb with mean concentration 0.090 ± 0.180 μg/kg and 0.000 ± 0.000 μg/kg for processed and local butter samples, 0.350 ± 0.606 μg/kg and 0.000 ± 0.000 μg/kg for processed and local cheese samples, 0.000 ± 0.000 μg/kg and 0.542 ± 1.085 μg/kg for processed and local cream samples and 0.552 ± 1.001 μg/kg and 0.000 ± 0.000 μg/kg for processed and local yogurt samples, respectively. Moreover, milk samples showed highest AFM1 (62%) in local unprocessed dairy farm followed by samples from local milk shop (51%) and commercial dairy farm (31%). In addition, therapeutic efficacy of three different types of toxin binders showed that the toxin binder which had yeast wall (75%) and algae (25%) is the best to control AFM1 under field conditions. Overall, results of this study are valuable for dairy farmers on one hand and law enforcement authorities on the other to comprehend and control AFM1 problem in milk and main dairy products.

Journal of Bacteriology & Parasitology, Jul 23, 2018

Pakistan Journal of Zoology, 2010

The prevalence of the blood protozoa was studied by sampling from Sahiwal and Friesian cattle, 50... more The prevalence of the blood protozoa was studied by sampling from Sahiwal and Friesian cattle, 500 each, from livestock farms in three districts of Punjab province during September 2006 to August 2007. The seasonal prevalence was higher in Friesian cattle as compared with Sahiwal cattle i.e. 97.6% versus 44.8 % during summer, 17.6% versus 20.8% during winter, 12.8% versus 9.6% during autumn respectively. Initial blood film examination revealed anemia, thrombocytosis, and leukocytosis and hematological findings indicated macrocytic hypochromic anemia. Theileria annulata, Babesia bigemina and Babesia bovis were detected by PCR in both Friesian and Sahiwal cattle. Out of 500 blood samples obtained from each breed of cattle 5%, Sahiwal cattle were positive in blood smears for Theileria as against 7% samples of Friesian cattle. Blood protozoan mixed infection with Babesia 1.58% was recorded in both breeds by blood smear examination during summer and spring season. On the basis of PCR 23% prevalence in Sahiwal cattle was recorded for Theileria annulata compared to 40.4% prevalence in Friesian cattle, while 19% prevalence for Babesia was recorded in both breeds during all seasons. The result of IFA test indicated 19% prevalence in Sahiwal cattle for Theileria annulata compared with 20% in Friesian cattle, while mixed prevalence of 8% was recorded in both breeds during summer and spring season. PCR test was concluded as more sensitive and specific as compared to IFA test.

Pakistan Journal of Zoology, 2022

Borrelia burgdorferi sensu lato is the etiological agent of Lyme disease. We investigated presenc... more Borrelia burgdorferi sensu lato is the etiological agent of Lyme disease. We investigated presence of B. burgdorferi s.l. in 600 pet dogs and 391 tick pools using PCR assay and sequencing in the Lahore City Metropolitan Area, Pakistan. Potential association of various risk factors with occurrence of Lyme borreliosis was also estimated via univariate and multivariate logistic regression. From each dog, blood and tick samples were collected and then tick species were identified. DNA extraction was followed by detection of 16S rRNA signature gene using B. burgdorferi s.l. specific primers through conventional PCR. We found that 4.3% dogs and 8.9% tick pools were positive for B. burgdorferi s.l. Rhipicephalus sanguineus (86.5%) was the most abundant tick species. 57.1% I. gibbosus and 8.4% R. sanguineus pools tested positive for B. burgdorferi s.l. Phylogenetically, our sequences clustered with B. burgdorferi sensu stricto, B. bavariensis, B. garinii, and B. bissettii sequences sourced from different hosts worldwide. Sequences showed 40.2%-99.1% sequences identity among them and 39%-98.3% with previously reported sequences on NCBI GenBank. Our sequences were submitted in NCBI GenBank under accession numbers MW547399-MW547405. Four animal-related: age (p=0.03), weight (p<0.05), sex (p=0.03), and breed (p<0.05), while three management-related risk factors: type of housing (p<0.05), tick infestation (p=0.007), and travel history (p=0.003) were significantly (p < 0.05) associated with occurrence of Lyme borreliosis. This is the first report on the presence of B. burgdorferi s.l. in Pakistan indicating the need for more comprehensive molecular surveys to estimate its prevalence in wider geographical areas and additional animal species as well as human population.

Livestock Science, Sep 1, 2021

Abstract This study aimed to compare therapeutic efficacy of procaine penicillin, Phyllanthus emb... more Abstract This study aimed to compare therapeutic efficacy of procaine penicillin, Phyllanthus emblica fruit extract and Cocos nucifera oil against subclinical mastitis, and its effects on milk composition. A total of 30 lactating beetal goats were selected, and divided into three groups (GA, GB and GC), each having 10 goats. GA was treated with procaine penicillin and GB with Phyllanthus emblica fruit extract while, GC with Cocos nucifera oil for five consecutive days. The milk sampling was done at pre-treatment (zero day), and post-treatment trial (7th and 14th day) aseptically. The evaluation parameters were CMT and bacteriological infection-based cure rate, milk pH, specific gravity, titratable acidity, electrical conductivity, total SCC, ALP and AP enzymes, as well as total milk yield. The cure rate of sub-clinically mastitic teats, based on CMT results was highest in GA (60%, 70%), moderate in GC (50%, 70%) and lowest in GB (50%, 60%) at 7th and 14th day respectively, while bacteriological infection-based cure rate was highest in GA (50%, 60%), followed by GC (40%, 70%) and GB (40%, 50%) at 7th and 14th day respectively. Further, with the treatment of Phyllanthus emblica fruit extract and Cocos nucifera oil, pH, electrical conductivity, total SCC, titratable acidity, ALP and AP enzymes of milk decreases, while milk fat and protein, specific gravity as well as total milk yield increases with time from zero day to 14th day in all three groups (P

Andrologia, Jan 14, 2016

The effects of l-cysteine in extender on antioxidant enzymes profile during cryopreservation, pos... more The effects of l-cysteine in extender on antioxidant enzymes profile during cryopreservation, post-thaw quality parameters and in vivo fertility of Nili-Ravi buffalo bull spermatozoa were studied. Semen samples from 4 buffalo bulls were diluted in Tris-citric acid-based extender having different concentrations of l-cysteine (0.0, 0.5, 1.0, 2.0 and 3.0 mm) and frozen in 0.5-ml French straws. The antioxidative enzymes [catalase, super oxide dismutase and total glutathione (peroxidase and reductase)] were significantly higher (P &amp;amp;amp;amp;amp;lt; 0.05) at pre-freezing and post-thawing in extender containing 2.0 mm l-cysteine as compared to other groups. Post-thaw total motility (%), progressive motility (%), rapid velocity (%), average path velocity (μm s(-1) ), straight line velocity (μm s(-1) ), curvilinear velocity (μm s(-1) ), beat cross frequency (Hz), viable spermatozoa with intact plasmalemma (%), acrosome and DNA integrity (%) were higher with the addition of 2.0 mm l-cysteine as compared to other groups (P &amp;amp;amp;amp;amp;lt; 0.05). The fertility rates (59 versus 43%) were higher (P &amp;amp;amp;amp;amp;lt; 0.05) in buffaloes inseminated with doses containing 2.0 mm of l-cysteine than in the control. In conclusion, the addition of 2.0 mm l-cysteine in extender improved the antioxidant enzymes profile, post-thaw quality and in vivo fertility of Nili-Ravi buffalo bull spermatozoa.

Punjab University Journal of Zoology, 2021

The aim of the present review was to study about the CRISPR Cas system and there role in genome e... more The aim of the present review was to study about the CRISPR Cas system and there role in genome editing, bacterial virulence and antibiotics resistance. CRISPR Cas system is an integral part of prokaryotic (Bacteria and Archaea) immune system that provides protection against viral infection. When bacteria recognize viral DNA inside it, bacteria incorporate small fragment of viral DNA into its genome at specific site termed as CRISPR locus. Insertion of viral DNA at CRISPR locus allows to remember, diagnose and clear the viral infection by the mechanism of sequence specific Adaptive Immunity. CRISPR Cas system is sustainable to combate with the mutation developed in viral genome that help viruses to escape from bacterial CRISPR Cas based immune system. CRISPR Cas system is a molecular mechanism of prokaryotic microorganism. It acts as bacterial natural adaptive immune system against phages, plasmids and foreign genomic elements. Mostly prokaryotes uses their CRIPR Cas system to enhance the integrity of their cell membrane that inhibit the permeability of antimicrobials from host body into the bacterial cells. CRISPR Cas system also help bacteria to evade from the host immune system by suppressing the activity of their immune receptors e.g. TLR. CRISPR Cas system also help bacteria to attach with the host body and replicate with in host body. It can develop antibiotic resistance in pathogenic bacteria, enhance their pathogenicity and can survival in host body. Novelty Statement | CRISPR Cas system is an integral part of prokaryotic immune system that provides protection against viral infection and involved in genome editing, bacterial virulence and antibiotics resistance.

Biomedical Journal of Scientific & Technical Research, 2017

Antimicrobial resistance (AMR) is major problem in treatment of uterine infections in dairy anima... more Antimicrobial resistance (AMR) is major problem in treatment of uterine infections in dairy animals. To suggest an alternative treatment, avoiding antibiotics, the effect of ozone as therapy against common bacterial uterine infections in dairy females the present study was carried out in 50 animals at dairy farm in district Sarghoda, Punjab province, Pakistan. The animals with history of repeat breeding and abnormal uterine secretions were selected and randomly divided in two groups. Group A received commercially prepared Ozone(Sanofoam®) intrauterine treatment while group B wasgiven gentamicin sulphate(Gentafar 10%®)@30gm in 1000 ml saline water treat intrauterine. Uterine lavage was taken twice, once before applying treatment and second after 8 hours of applying treatment. Samples were cultured for bacteriology to detect E.coli, F. necrophorum, A. pyogenes and St. pyogenes. Bacteria wise percentage of uterine infection was highest for E.coli (88%) followed by 84%, 68% and 60% for A. pyogenes, F. necrophorum, and St. pyogenes respectively. Difference of the colony forming units before and after applying both treatments for each bacterium was calculated. Results were interpreted statistically. Differences among the groups were considered significant at P < 0.05. E. coli, F.necrophorum and St. pyogenes were highly significant as the P value for group differences was less than 0.05. Group differences among St.pyogenes showed no significance as the P>0.05. The results showed that intrauterine treatment with ozone was more responsive (38/50, 76%) as compared to gentamicin sulphate intrauterine treatment (37/50,74%) in cross bred dairy cows with bacterial infections.

Journal of Animal and Plant Sciences

Study was carried out to evaluate the pathological effects of parenterally administrated “Calotro... more Study was carried out to evaluate the pathological effects of parenterally administrated “Calotropis procera extracts” in rabbits. Fresh flowery buds of C. procera were collected from the District Lahore, Pakistan. Total of 35 rabbits of either sex were purchased from the animal house of University of Veterinary and Animal Sciences, Lahore. Rabbits were randomly distributed in to 3 major groups (A= C. procera chloroform extract, B= C. procera water extract, C= Normal saline). Groups A and B were further subdivided into three sub-groups (A1, A2, A3, B1, B2 and B3) of 5 and C (n=5) were used as a control group. Biochemical findings showed a significant increase (P<0.05) in serum level of creatinine,urea and ALT in group A3 at day 30 as compared with group C. Gross lesions consisting of severe hemorrhages, lung emphysema in group A3 and lobular congestion, multifocal emphysema were observed in group B3. Histopathological lesions such as hyperplasia of Kupffer cells, hepatic cell deg...

Pakistan Journal of Zoology, May 11, 2018

Pakistan Journal of Zoology, 2018

Authors' Contributions AZD, MSK, MHM and IH designed the study. IH and AZD executed the experimen... more Authors' Contributions AZD, MSK, MHM and IH designed the study. IH and AZD executed the experiment and analysed the samples. IA, AK and MA helped in data analysis and article drafting.

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2018

Clostridium perfringens (C. perfringens) causes disease, generally, named as enterotoxemia in the... more Clostridium perfringens (C. perfringens) causes disease, generally, named as enterotoxemia in the animals. This bacterium is a normal inhabitant in gastro-intestinal tract (GIT) of animals and become harmful by increasing its colony counts as well as toxin liberation whenever gets an opportunity of favorable conditions. This study focused molecular typing of C. perfringens (α, β, ε, ι toxins) and type 'A' multidrug resistance profile in diarrheic goats in Pakistan. Diarrheic fecal samples (n=192) were collected from goats and 80.73% (155/192) of the samples were found positive for C. perfringens on the basis of culture growth and PCR. Elevated C. perfringens counts (>10 7 CFU/g) were recorded in 33.55% (52/155) of positive samples, while, 66.45% (103/155) of the positive sample appeared in normal range of bacterial counts (10 4-10 7 CFU/g). Molecular detection was carried out by targeting specific toxin genes i.e. cpa (α), cpb (β), etx (ε) and iap (ι) of C. perfringens using PCR. Among the positive samples, 78.06% of the isolates were found as type 'A' , 5.16% isolates were type 'B' , 3.23% isolates were type 'C' while, 13.55% of the positive samples were type 'D' C. perfringens. None of the isolates was found positive for iap toxin gene (type 'E'). C. perfringens type 'A' was subjected to in-vitro antibiotic sensitivity test. Penicillin, ciprofloxacin and ceftriaxone were found sensitive while bacitracin, amoxicillin and ampicillin were found least sensitive antibiotics. This study concludes that C. perfringens type 'A' is highly prevalent among goats in Pakistan and clinical cases of enterotoxemia can be effectively dealt with penicillin, ciprofloxacin and ceftriaxone antibiotics.

Kafkas Universitesi Veteriner Fakultesi Dergisi, 2017

Poultry Science, 2011

Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on ... more Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre-including this research content-immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

Pakistan Journal of Zoology, 2021

Authors' Contribution SA, MA and AZD conceived the research idea and designed the study. SA, SJ a... more Authors' Contribution SA, MA and AZD conceived the research idea and designed the study. SA, SJ and MA visited the dairy farms and collected the data. SH and IA performed data analysis. MA, KA and JAK wrote the paper.