Jesus Quiñónez - Profile on Academia.edu (original) (raw)

Papers by Jesus Quiñónez

Aquaculture Research, 2009

We evaluated the performance of whiteleg shrimp Penaeus vannamei (Boone, 1931) in response to dif... more We evaluated the performance of whiteleg shrimp Penaeus vannamei (Boone, 1931) in response to different stocking densities and acclimation periods. Shrimp postlarvae were acclimated from seawater (30 g L−1) to low-salinity well water (<1.0 g L−1) at a constant hourly reduction rate of 40, 60, 80 and 100 h. After acclimation to low-salinity well water, postlarvae from each acclimation time treatment were stocked in three replicate tanks at densities of 50, 100, 150 or 200 shrimps m−2 for 12 weeks of growth. Salinity averaged <1.0 g L−1 for each growth study. The different treatments resulted in significant differences in both the final body weight and the survival rate (SR). Shrimp acclimated for 100 h showed substantially improved survival (83%) relative to shrimp acclimated for shorter periods. Shrimp yields for all cultured periods ranged from 0.32 kg m−2 in tanks stocked at 50 m−2 to 1.14 kg m−2 in tanks stocked at 200 m−2. We conclude that whiteleg shrimp can be successfully grown in low-salinity well water, and that the growth, production output and SRs are significantly higher when shrimp are acclimated for longer periods.

Journal of The World Aquaculture Society, 2010

Inflammation Research, 2006

Background Negative results are frequent using anti-TNFα antibodies in sepsis models and clinical... more Background Negative results are frequent using anti-TNFα antibodies in sepsis models and clinical trials. Methods and Results Different prophylactic doses of anti-TNFα F(ab')2 antibody fragments were compared for the prevention of death by sepsis induced by cecal ligation and puncture (CLP) in mice. High (10 mg/kg) and very low (0.01 and 0.1 mg/kg) concentrations of anti-TNFα antibody fragments were not the most adequate for treating polymicrobial sepsis, since they did not significantly improve survival. To the contrary, intermediate doses (1 mg/kg) significantly protected the challenged animals. Protective activity was also observed when administration of the antibody fragments was initiated early (up to 30 min) after CLP. Conclusions These results suggest that in processes where excessive production of cytokines is involved, the aim should be to return them to their physiologically acting range but not to inhibit their production. The timing of initiating therapy should also be considered in order to maximize the possible benefits.

Experimental Cell Research, 1999

Most of the information on the structure and function of the tight junction (TJ) has been obtaine... more Most of the information on the structure and function of the tight junction (TJ) has been obtained in MDCK cells. Accordingly, we have sequenced ZO-1 in this cell type, because this protein is involved in the response of the TJ to changes in Ca 2؉ , phosphorylation, and the cytoskeleton. ZO-1 of MDCK cells comprises 6805 bp with a predicted open reading frame of 1769 amino acids. This sequence is 92 and 87% homologous to human and mouse ZO-1, respectively. Two nuclear sorting signals located at the PDZ1 and GK domains and 17 SH3 putative binding sites at the proline-rich domain were detected. We found two new splicing regions at the proline-rich region: ␤ had not been reported in human and mouse counterparts, and ␥, which was previously sequenced in human and mouse ZO-1, is now identified as a splicing region. The expression of different ␤ and ␥ isoforms varies according to the tissue tested. With the information provided by the sequence, Southern blot, and PCR experiments we can predict a single genomic copy of MDCK-ZO-1 that is at least 13.16 kb long. MDCK-ZO-1 mRNA is 7.4 kb long. Its expression is regulated by calcium, while the expression of MDCK-ZO-1 protein is not.

A detailed study is presented of the expected performance of the ATLAS detector. The reconstructi... more A detailed study is presented of the expected performance of the ATLAS detector. The reconstruction of tracks, leptons, photons, missing energy and jets is investigated, together with the performance of b-tagging and the trigger. The physics potential for a variety of interesting physics processes, within the Standard Model and beyond, is examined. The study comprises a series of notes based on simulations of the detector and physics processes, with particular emphasis given to the data expected from the first years of operation of the LHC at CERN.

European Food Research and Technology, 2006

The purpose of this work was to study the influence of the industrial process steps on free fatty... more The purpose of this work was to study the influence of the industrial process steps on free fatty acids, peroxide value (PV), p-anisidine value (PAV), trans fatty acids, tocopherols and sterols (free, esterified and total) in high oleic safflower oil. Degumming, bleaching and deodorization steps removed 91.4% of free fatty acids, 96.31% of oxidation primary products (PV), and 54.57% of oxidation secondary products (PAV), from crude high oleic safflower oil. Degumming neither affected the content of sterified sterols nor its proportion with respect to the crude oil. A significant increment (p<0.05) in the content of free sterols was observed during degumming and bleaching due to the acid-catalyzed hydrolysis of steryl esters. A significant reduction (p<0.05) in the content of total sterols during bleaching was observed, which is attributed to a reduction in the sterified sterol fraction. During deodorization, free sterols were distilled from oil, with a gradual reduction in the total sterol content as a function of the deodorization temperature. α- and γ-tocopherols represented 93.3% of the total tocopherols in high oleic safflower crude oil. The refining process removed 28.5% of the tocopherols. Deodorization was the main step which increased the level of trans fatty acids as an effect of temperature and heating time.

Journal of Applied Aquaculture, 2009

The effects of four different ionic composition low salinity water (T1, T2, T3, and T4), on growt... more The effects of four different ionic composition low salinity water (T1, T2, T3, and T4), on growth and survival of Litopenaeus vannamei marine shrimp juveniles were investigated. Shrimp culture in seawater (Tm) was used as control treatment. The results indicated that there were no significant difference (P < 0.05) in growth, survival, production, and feed conversion ratio (FCR) of L. vannamei juveniles reared in the different treatments, but significant differences (P < 0.05) were observed between each of them when compared with seawater (Tm). After 84 days, culture shrimp grew from 0.02 to 7.58 g in T1. The lowest growth rate was attained in T3 (0.57 g/week), in which potassium and calcium ions concentrations were the lowest (0.58 and 28.00 mg/L, respectively). The recorded survival rate (76.35% to 79.55%) is considered well for the 84 days growout period, although it was 7.6% lower than that recorded in the control treatment. Although there were no significant differences (P < 0.05) in growth with respect to the ionic composition of the four treatments, there was a trend of increasing growth in relation with the ionic ratio found in the seawater (Tm). This aspect should be evaluated more closely in future research.

La misión es el motivo, propósito, fin o razón de ser de la existencia de una empresa u organizac... more La misión es el motivo, propósito, fin o razón de ser de la existencia de una empresa u organización porque define: 1) lo que pretende cumplir en su entorno o sistema social en el que actúa, 2) lo que pretende hacer, y 3) el para quién lo va a hacer; y es influenciada en momentos concretos por algunos elementos como: la historia de la organización, las preferencias de la gerencia y/o de los propietarios, los factores externos o del entorno, los recursos disponibles, y sus capacidades distintivas [1].

Aquaculture Research, 2009

We evaluated the performance of whiteleg shrimp Penaeus vannamei (Boone, 1931) in response to dif... more We evaluated the performance of whiteleg shrimp Penaeus vannamei (Boone, 1931) in response to different stocking densities and acclimation periods. Shrimp postlarvae were acclimated from seawater (30 g L−1) to low-salinity well water (<1.0 g L−1) at a constant hourly reduction rate of 40, 60, 80 and 100 h. After acclimation to low-salinity well water, postlarvae from each acclimation time treatment were stocked in three replicate tanks at densities of 50, 100, 150 or 200 shrimps m−2 for 12 weeks of growth. Salinity averaged <1.0 g L−1 for each growth study. The different treatments resulted in significant differences in both the final body weight and the survival rate (SR). Shrimp acclimated for 100 h showed substantially improved survival (83%) relative to shrimp acclimated for shorter periods. Shrimp yields for all cultured periods ranged from 0.32 kg m−2 in tanks stocked at 50 m−2 to 1.14 kg m−2 in tanks stocked at 200 m−2. We conclude that whiteleg shrimp can be successfully grown in low-salinity well water, and that the growth, production output and SRs are significantly higher when shrimp are acclimated for longer periods.

Journal of The World Aquaculture Society, 2010

Inflammation Research, 2006

Background Negative results are frequent using anti-TNFα antibodies in sepsis models and clinical... more Background Negative results are frequent using anti-TNFα antibodies in sepsis models and clinical trials. Methods and Results Different prophylactic doses of anti-TNFα F(ab')2 antibody fragments were compared for the prevention of death by sepsis induced by cecal ligation and puncture (CLP) in mice. High (10 mg/kg) and very low (0.01 and 0.1 mg/kg) concentrations of anti-TNFα antibody fragments were not the most adequate for treating polymicrobial sepsis, since they did not significantly improve survival. To the contrary, intermediate doses (1 mg/kg) significantly protected the challenged animals. Protective activity was also observed when administration of the antibody fragments was initiated early (up to 30 min) after CLP. Conclusions These results suggest that in processes where excessive production of cytokines is involved, the aim should be to return them to their physiologically acting range but not to inhibit their production. The timing of initiating therapy should also be considered in order to maximize the possible benefits.

Experimental Cell Research, 1999

Most of the information on the structure and function of the tight junction (TJ) has been obtaine... more Most of the information on the structure and function of the tight junction (TJ) has been obtained in MDCK cells. Accordingly, we have sequenced ZO-1 in this cell type, because this protein is involved in the response of the TJ to changes in Ca 2؉ , phosphorylation, and the cytoskeleton. ZO-1 of MDCK cells comprises 6805 bp with a predicted open reading frame of 1769 amino acids. This sequence is 92 and 87% homologous to human and mouse ZO-1, respectively. Two nuclear sorting signals located at the PDZ1 and GK domains and 17 SH3 putative binding sites at the proline-rich domain were detected. We found two new splicing regions at the proline-rich region: ␤ had not been reported in human and mouse counterparts, and ␥, which was previously sequenced in human and mouse ZO-1, is now identified as a splicing region. The expression of different ␤ and ␥ isoforms varies according to the tissue tested. With the information provided by the sequence, Southern blot, and PCR experiments we can predict a single genomic copy of MDCK-ZO-1 that is at least 13.16 kb long. MDCK-ZO-1 mRNA is 7.4 kb long. Its expression is regulated by calcium, while the expression of MDCK-ZO-1 protein is not.

A detailed study is presented of the expected performance of the ATLAS detector. The reconstructi... more A detailed study is presented of the expected performance of the ATLAS detector. The reconstruction of tracks, leptons, photons, missing energy and jets is investigated, together with the performance of b-tagging and the trigger. The physics potential for a variety of interesting physics processes, within the Standard Model and beyond, is examined. The study comprises a series of notes based on simulations of the detector and physics processes, with particular emphasis given to the data expected from the first years of operation of the LHC at CERN.

European Food Research and Technology, 2006

The purpose of this work was to study the influence of the industrial process steps on free fatty... more The purpose of this work was to study the influence of the industrial process steps on free fatty acids, peroxide value (PV), p-anisidine value (PAV), trans fatty acids, tocopherols and sterols (free, esterified and total) in high oleic safflower oil. Degumming, bleaching and deodorization steps removed 91.4% of free fatty acids, 96.31% of oxidation primary products (PV), and 54.57% of oxidation secondary products (PAV), from crude high oleic safflower oil. Degumming neither affected the content of sterified sterols nor its proportion with respect to the crude oil. A significant increment (p<0.05) in the content of free sterols was observed during degumming and bleaching due to the acid-catalyzed hydrolysis of steryl esters. A significant reduction (p<0.05) in the content of total sterols during bleaching was observed, which is attributed to a reduction in the sterified sterol fraction. During deodorization, free sterols were distilled from oil, with a gradual reduction in the total sterol content as a function of the deodorization temperature. α- and γ-tocopherols represented 93.3% of the total tocopherols in high oleic safflower crude oil. The refining process removed 28.5% of the tocopherols. Deodorization was the main step which increased the level of trans fatty acids as an effect of temperature and heating time.

Journal of Applied Aquaculture, 2009

The effects of four different ionic composition low salinity water (T1, T2, T3, and T4), on growt... more The effects of four different ionic composition low salinity water (T1, T2, T3, and T4), on growth and survival of Litopenaeus vannamei marine shrimp juveniles were investigated. Shrimp culture in seawater (Tm) was used as control treatment. The results indicated that there were no significant difference (P < 0.05) in growth, survival, production, and feed conversion ratio (FCR) of L. vannamei juveniles reared in the different treatments, but significant differences (P < 0.05) were observed between each of them when compared with seawater (Tm). After 84 days, culture shrimp grew from 0.02 to 7.58 g in T1. The lowest growth rate was attained in T3 (0.57 g/week), in which potassium and calcium ions concentrations were the lowest (0.58 and 28.00 mg/L, respectively). The recorded survival rate (76.35% to 79.55%) is considered well for the 84 days growout period, although it was 7.6% lower than that recorded in the control treatment. Although there were no significant differences (P < 0.05) in growth with respect to the ionic composition of the four treatments, there was a trend of increasing growth in relation with the ionic ratio found in the seawater (Tm). This aspect should be evaluated more closely in future research.

La misión es el motivo, propósito, fin o razón de ser de la existencia de una empresa u organizac... more La misión es el motivo, propósito, fin o razón de ser de la existencia de una empresa u organización porque define: 1) lo que pretende cumplir en su entorno o sistema social en el que actúa, 2) lo que pretende hacer, y 3) el para quién lo va a hacer; y es influenciada en momentos concretos por algunos elementos como: la historia de la organización, las preferencias de la gerencia y/o de los propietarios, los factores externos o del entorno, los recursos disponibles, y sus capacidades distintivas [1].