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Papers by Mireille Kameni

Journal of Ethnopharmacology, 2015

Gouania longipetala is commonly used in Cameroonian traditional medicine to manage women fertilit... more Gouania longipetala is commonly used in Cameroonian traditional medicine to manage women fertility and menopausal complaints. However, despite this use, the estrogenic properties of Gouania longipetala (G. longipetala) have not been studied until now. The present study was aimed to assess estrogenic activities of the stem bark aqueous (GLA) and ethanolic (GLE) extracts of G. longipetala in post menopause-like model of ovariectomized (Ovx) Wistar rats. Animals were either sham-operated or Ovx. 84 days after ovariectomy, animals were divided into seven groups of five animals and were daily treated for 28 days with distilled water (10mL/kg) for group 1, 2% solution of Tween 80 (10mL/kg) for group 2, estradiol valerate (1mg/kg) for group 3, GLA (45 or 180mg/kg) and GLE (40 or 160mg/kg) for groups 4 to 7 respectively. Sham-operated animals daily received distilled water (10mL/kg). During the experimental period, the body weight was registered every week. At the day 29, blood pressure was registered by invasive method while uterine and vagina morphometry as well as body, uterine and abdominal fat weights changes were analyzed. Serum levels of total cholesterol, triglycerides, HDL-cholesterol and LDL-cholesterol were determined. Moreover, oxidative stress markers such as nitrites, reduced glutathione (GSH) and malondialdehyde (MDA) were measured in homogenized liver and aorta. Compared with the sham control, vagina and uterine dystrophy and elevated blood pressure were observed in Ovx rats treated with vehicles. Biochemical parameters showed a significant increase of total cholesterol, triglycerides, LDL-cholesterol and MDA as well as a significant decrease of nitrites and GSH in Ovx animals treated with vehicle as compared to sham group. GLA and GLE displayed estrogen-like effects on vagina and did not affect uterine wet weight and epithelial height compared with vehicle groups. Both extracts displayed anti-atherogenic properties by reducing AI, AIP and LDL-cholesterol level as compared to vehicles groups. GLA and GLE significantly prevented the increase of MDA induced by ovariectomy as compared to rats treated with vehicles. This study showed that GLA and GLE exhibited estrogenic effects by providing vaginal lubrication, by modulating blood pressure and improving lipid profile, oxidative status and endothelial function and may not have an undesirable influence on the endometrium in ovariectomized rats.

Evidence-Based Complementary and Alternative Medicine

Hypertension (HT) is a risk factor for erectile dysfunction (ED). This study aimed to evaluate th... more Hypertension (HT) is a risk factor for erectile dysfunction (ED). This study aimed to evaluate the suppressive effect of Nymphaea lotus (N. lotus) on erectile dysfunction induced by NO deficiency in rat. 40 male rats equally divided into 4 groups received an oral treatment with 10 mg/kg/day of L-NAME, a NO blocker, during 4 weeks. Control group composed of 10 male rats received only distilled water (10 mL/kg). Thereafter oral treatments with N. lotus (75 and 200 mg/kg/day) and losartan (10 mg/kg/day) started and continued concomitantly with L-NAME in 3 groups for 4 additional weeks. Normal and negative controls received only distilled water. Sexual behaviour, orientation activities, anxiety, and penile histomorphology were evaluated at the end of treatment. L-NAME administration elevated significantly the blood pressure in male rats and decreased the copulatory rate by enhancing intromission latency and decreasing the numbers of intromission and ejaculation. However, the sexual moti...

Journal of ethnopharmacology, Jan 4, 2015

Gouania longipetala is commonly used in Cameroonian traditional medicine to manage women fertilit... more Gouania longipetala is commonly used in Cameroonian traditional medicine to manage women fertility and menopausal complaints. However, despite this use, the estrogenic properties of Gouania longipetala (G. longipetala) have not been studied until now. The present study was aimed to assess estrogenic activities of the stem bark aqueous (GLA) and ethanolic (GLE) extracts of G. longipetala in post menopause-like model of ovariectomized (Ovx) Wistar rats. Animals were either sham-operated or Ovx. 84 days after ovariectomy, animals were divided into seven groups of five animals and were daily treated for 28 days with distilled water (10mL/kg) for group 1, 2% solution of Tween 80 (10mL/kg) for group 2, estradiol valerate (1mg/kg) for group 3, GLA (45 or 180mg/kg) and GLE (40 or 160mg/kg) for groups 4 to 7 respectively. Sham-operated animals daily received distilled water (10mL/kg). During the experimental period, the body weight was registered every week. At the day 29, blood pressure was...

Journal of Complementary and Integrative Medicine

We investigated the preventive effect of the aqueous extract of Milicia excelsa (M. excelsa) on i... more We investigated the preventive effect of the aqueous extract of Milicia excelsa (M. excelsa) on insulin
resistance induced by dexamethasone in rat. Insulin resistance was induced by intraperitoneal injection of
dexamethasone (200 μg/kg) during 21 days. The animals were divided into 5 groups 6 in each. Treated groups
received concomitantly with dexamethasone, the plant extract (50 and 100 mg/kg) or metformin (200 mg/kg).
At the end of the treatment, blood glucose, plasma insulin, aspartate and alanine transaminase (AST and ALT),
urea, creatinin and lipid profile were estimated. HOMA-IR index was calculated as a marker of insulin
resistance. M. excelsa significantly prevented the body weight lost, the increase in blood glucose, insulin,
triglyceride, total-cholesterol and ALT. The index of insulin resistance was significantly reduced (P<0.001) by
57.53 and 66.64% with M. excelsa at the doses of 50 and 100 mg/kg, respectively as compared to
dexamethasone. At the same doses, the plant extract significantly prevented the rise of blood glucose 2 hours
after glucose load by 15.43 and 18.67 %, respectively, as compared to dexamethasone. These data indicated
that M. excelsa aqueous extract might prevent the development of dexamethasone-induced insulin resistance
and related abnormalities in rats.

Conference Presentations by Mireille Kameni

held in RDC, Kinshasa, 5-6 september 2014

Journal of Ethnopharmacology, 2015

Gouania longipetala is commonly used in Cameroonian traditional medicine to manage women fertilit... more Gouania longipetala is commonly used in Cameroonian traditional medicine to manage women fertility and menopausal complaints. However, despite this use, the estrogenic properties of Gouania longipetala (G. longipetala) have not been studied until now. The present study was aimed to assess estrogenic activities of the stem bark aqueous (GLA) and ethanolic (GLE) extracts of G. longipetala in post menopause-like model of ovariectomized (Ovx) Wistar rats. Animals were either sham-operated or Ovx. 84 days after ovariectomy, animals were divided into seven groups of five animals and were daily treated for 28 days with distilled water (10mL/kg) for group 1, 2% solution of Tween 80 (10mL/kg) for group 2, estradiol valerate (1mg/kg) for group 3, GLA (45 or 180mg/kg) and GLE (40 or 160mg/kg) for groups 4 to 7 respectively. Sham-operated animals daily received distilled water (10mL/kg). During the experimental period, the body weight was registered every week. At the day 29, blood pressure was registered by invasive method while uterine and vagina morphometry as well as body, uterine and abdominal fat weights changes were analyzed. Serum levels of total cholesterol, triglycerides, HDL-cholesterol and LDL-cholesterol were determined. Moreover, oxidative stress markers such as nitrites, reduced glutathione (GSH) and malondialdehyde (MDA) were measured in homogenized liver and aorta. Compared with the sham control, vagina and uterine dystrophy and elevated blood pressure were observed in Ovx rats treated with vehicles. Biochemical parameters showed a significant increase of total cholesterol, triglycerides, LDL-cholesterol and MDA as well as a significant decrease of nitrites and GSH in Ovx animals treated with vehicle as compared to sham group. GLA and GLE displayed estrogen-like effects on vagina and did not affect uterine wet weight and epithelial height compared with vehicle groups. Both extracts displayed anti-atherogenic properties by reducing AI, AIP and LDL-cholesterol level as compared to vehicles groups. GLA and GLE significantly prevented the increase of MDA induced by ovariectomy as compared to rats treated with vehicles. This study showed that GLA and GLE exhibited estrogenic effects by providing vaginal lubrication, by modulating blood pressure and improving lipid profile, oxidative status and endothelial function and may not have an undesirable influence on the endometrium in ovariectomized rats.

Evidence-Based Complementary and Alternative Medicine

Hypertension (HT) is a risk factor for erectile dysfunction (ED). This study aimed to evaluate th... more Hypertension (HT) is a risk factor for erectile dysfunction (ED). This study aimed to evaluate the suppressive effect of Nymphaea lotus (N. lotus) on erectile dysfunction induced by NO deficiency in rat. 40 male rats equally divided into 4 groups received an oral treatment with 10 mg/kg/day of L-NAME, a NO blocker, during 4 weeks. Control group composed of 10 male rats received only distilled water (10 mL/kg). Thereafter oral treatments with N. lotus (75 and 200 mg/kg/day) and losartan (10 mg/kg/day) started and continued concomitantly with L-NAME in 3 groups for 4 additional weeks. Normal and negative controls received only distilled water. Sexual behaviour, orientation activities, anxiety, and penile histomorphology were evaluated at the end of treatment. L-NAME administration elevated significantly the blood pressure in male rats and decreased the copulatory rate by enhancing intromission latency and decreasing the numbers of intromission and ejaculation. However, the sexual moti...

Journal of ethnopharmacology, Jan 4, 2015

Gouania longipetala is commonly used in Cameroonian traditional medicine to manage women fertilit... more Gouania longipetala is commonly used in Cameroonian traditional medicine to manage women fertility and menopausal complaints. However, despite this use, the estrogenic properties of Gouania longipetala (G. longipetala) have not been studied until now. The present study was aimed to assess estrogenic activities of the stem bark aqueous (GLA) and ethanolic (GLE) extracts of G. longipetala in post menopause-like model of ovariectomized (Ovx) Wistar rats. Animals were either sham-operated or Ovx. 84 days after ovariectomy, animals were divided into seven groups of five animals and were daily treated for 28 days with distilled water (10mL/kg) for group 1, 2% solution of Tween 80 (10mL/kg) for group 2, estradiol valerate (1mg/kg) for group 3, GLA (45 or 180mg/kg) and GLE (40 or 160mg/kg) for groups 4 to 7 respectively. Sham-operated animals daily received distilled water (10mL/kg). During the experimental period, the body weight was registered every week. At the day 29, blood pressure was...

Journal of Complementary and Integrative Medicine

We investigated the preventive effect of the aqueous extract of Milicia excelsa (M. excelsa) on i... more We investigated the preventive effect of the aqueous extract of Milicia excelsa (M. excelsa) on insulin
resistance induced by dexamethasone in rat. Insulin resistance was induced by intraperitoneal injection of
dexamethasone (200 μg/kg) during 21 days. The animals were divided into 5 groups 6 in each. Treated groups
received concomitantly with dexamethasone, the plant extract (50 and 100 mg/kg) or metformin (200 mg/kg).
At the end of the treatment, blood glucose, plasma insulin, aspartate and alanine transaminase (AST and ALT),
urea, creatinin and lipid profile were estimated. HOMA-IR index was calculated as a marker of insulin
resistance. M. excelsa significantly prevented the body weight lost, the increase in blood glucose, insulin,
triglyceride, total-cholesterol and ALT. The index of insulin resistance was significantly reduced (P<0.001) by
57.53 and 66.64% with M. excelsa at the doses of 50 and 100 mg/kg, respectively as compared to
dexamethasone. At the same doses, the plant extract significantly prevented the rise of blood glucose 2 hours
after glucose load by 15.43 and 18.67 %, respectively, as compared to dexamethasone. These data indicated
that M. excelsa aqueous extract might prevent the development of dexamethasone-induced insulin resistance
and related abnormalities in rats.

held in RDC, Kinshasa, 5-6 september 2014