Ekaterina Badaeva | Vavilov Institute of General Genetics (original) (raw)

Papers by Ekaterina Badaeva

Biology Bulletin, 2018

⎯The peculiarities of meiosis, C-heterochromatin (C-HC) distribution, and the activity of nucleol... more ⎯The peculiarities of meiosis, C-heterochromatin (C-HC) distribution, and the activity of nucleolus organizer regions (NORs) of the chromosomes were studied in the Pisum sativum L. pea and Triticum compactum Host. club wheat grown from the seeds obtained in Lada space greenhouse on the board of RS ISS. It was noted that the analysis of meiosis detected no significant violations in the "space" plant cells, and the total amount of C-HC did not differ significantly from the control, despite the polymorphism of the chromosome C-banding pattern. Paracentric inversion on the chromosome 4A was found for the first time in the T. compactum, while no additional active NOR chromosomes were found.

Frontiers in Plant Science

Emmer wheat, Triticum dicoccon SCHRANK (syn. T. dicoccum (SCHRANK) SCHÜBL.), is one of the earlie... more Emmer wheat, Triticum dicoccon SCHRANK (syn. T. dicoccum (SCHRANK) SCHÜBL.), is one of the earliest domesticated crops, harboring a wide range of genetic diversity and agronomi-cally valuable traits. The crop, however, is currently largely neglected. We provide a wealth of karyotypic information from a comprehensive collection of emmer wheat and related taxa. In addition to C-banding polymorphisms, we identified 43 variants of chromosomal re-arrangements in T. dicoccon; among them 26 (60.4%) were novel. The T7A:5B transloca-tion was most abundant in Western Europe and the Mediterranean. The plant genetic resources investigated here might become important in the future for wheat improvement. Based on cluster analysis four major karyotypic groups were discriminated within the T. dicoccon genepool, each harboring characteristic C-banding patterns and translocation spectra: the BALKAN, ASIAN, EUROPEAN and ETHIOPIAN groups. We postulate four major diffusion routes of the crop and discuss...

Deschampsia antarctica Desv. (Poaceae) (2n = 26) is one of the two vascular plants adapted to the... more Deschampsia antarctica Desv. (Poaceae) (2n = 26) is one of the two vascular plants adapted to the harshest environment of the Antarctic. Although the species is a valuable model for study of environmental stress tolerance in plants, its karyotype is still poorly inves-tigated. We firstly conducted a comprehensive molecular cytogenetic analysis of D. antarc-tica collected on four islands of the Maritime Antarctic. D. antarctica karyotypes were studied by Giemsa C- and DAPI/C-banding, Ag-NOR staining, multicolour fluorescence in situ hybridization with repeated DNA probes (pTa71, pTa794, telomere repeats, pSc119.2, pAs1) and the GAA simple sequence repeat probe. We also performed sequential rapid in situ hybridization with genomic DNA of D. caespitosa. Two chromosome pairs bearing tran-scriptionally active 45S rDNA loci and five pairs with 5S rDNA sites were detected. A weak intercalary site of telomere repeats was revealed on the largest chromosome in addition to telomere hybridizati...

Russian Journal of Plant Physiology, 1998

Theoretical and Applied Genetics

Introgressions from crop wild relatives (CWRs) have been used to introduce beneficial traits into... more Introgressions from crop wild relatives (CWRs) have been used to introduce beneficial traits into cultivated plants. Introgressions have traditionally been detected using cytological methods. Recently, single nucleotide polymorphism (SNP)-based methods have been proposed to detect introgressions in crosses for which both parents are known. However, for unknown material, no method was available to detect introgressions and predict the putative donor species. Here, we present a method to detect introgressions and the putative donor species. We demonstrate the utility of this method using 10 publicly available wheat genome sequences and identify nine major introgressions. We show that the method can distinguish different introgressions at the same locus. We trace introgressions to early wheat cultivars and show that natural introgressions were utilised in early breeding history and still influence elite lines today. Finally, we provide evidence that these introgressions harbour resista...

Plants, 2020

One of the limitations in obtaining the genetic diversity of doubled haploid (DH) lines via anthe... more One of the limitations in obtaining the genetic diversity of doubled haploid (DH) lines via anther culture is the development of families of regenerants, and each family represents a clone. This work examines the results of studying this phenomenon in anther culture of alloplasmic (H. vulgare)–T. aestivum and euplasmic lines with 1RS.1BL and 7DL-7Ai translocations and hybrids between them. Parameters of androgenesis such as the number of embryo-like structures, the total number of regenerants, and the number of green regenerants per 100 anthers varied depending on the genotype. In all genotypes from embryo-like structures, predominant development of families of plantlets rather than single plantlets was found. The source of family plantlets was polyembryos. About 75% of families consisted of regenerants at the same fertility level. On average, 37.74%4% of the R0 plants were fertile. The sister DH lines of three hybrid combinations were formed from seeds of R1 plants (2n = 42) with h...

Journal of Botany, 2010

Tetraploid oat species Avena abyssinica, A. vaviloviana, A. barbata, and A. agadiriana were studi... more Tetraploid oat species Avena abyssinica, A. vaviloviana, A. barbata, and A. agadiriana were studied using C-banding technique, in situ hybridization with the 45S and 5S rDNA probes, and RAPD analysis in comparison with the diploid species carrying different types of the A-genome (A. wiestii, As; A. longiglumis, Al; A. canariensis, Ac; A. damascena, Ad, A. prostrata, Ap). The investigation confirmed that all four tetraploids belong to the same AB-genome group; however A. agadiriana occupies distinct position among others. The C-banding, FISH, and RAPD analyses showed that Avena abyssinica, A. vaviloviana, and A. barbata are very similar; most probably they originated from a common tetraploid ancestor as a result of minor translocations and alterations of C-banding polymorphism system. AB-genome species are closely related with the A-genome diploids, and an As-genome species may be regarded as the most probable donor of their A-genome. Although their second diploid progenitor has not ...

Cytogenetic and genome research, Jan 4, 2015

Karyotypes of 3 diploid wheat species containing different variants of the A-genome, Triticum boe... more Karyotypes of 3 diploid wheat species containing different variants of the A-genome, Triticum boeoticum (A(b)), T. monococcum (A(b)), and T. urartu (A(u)), were examined using C-banding and FISH with DNA probes representing 5S and 45S rDNA families, the microsatellite sequences GAAn and GTTn, the already known satellite sequences pSc119.2, Spelt52, Fat, pAs1, and pTa535, and a newly identified repeat called Aesp_SAT86. The C-banding patterns of the 3 species in general were similar; differences were observed in chromosomes 4A and 6A. Besides 2 major 45S rDNA loci on chromosomes 1A and 5A, 2 minor polymorphic NORs were observed in the terminal part of 5AL and in the distal part of 6AS in all species. An additional minor locus was found in the distal part of 7A(b)L of T. boeoticum and T. monococcum, but not in T. urartu. Two 5S rDNA loci were observed in 1AS and 5AS. The pTa535 probe displayed species- and chromosome-specific hybridization patterns, allowing complete chromosome identi...

Plant Physiology, 2014

Bread wheat (Triticum aestivum) inflorescences, or spikes, are characteristically unbranched and ... more Bread wheat (Triticum aestivum) inflorescences, or spikes, are characteristically unbranched and normally bear one spikelet per rachis node. Wheat mutants on which supernumerary spikelets (SSs) develop are particularly useful resources for work towards understanding the genetic mechanisms underlying wheat inflorescence architecture and, ultimately, yield components. Here, we report the characterization of genetically unrelated mutants leading to the identification of the wheat FRIZZY PANICLE (FZP) gene, encoding a member of the APETALA2/Ethylene Response Factor transcription factor family, which drives the SS trait in bread wheat. Structural and functional characterization of the three wheat FZP homoeologous genes (WFZP) revealed that coding mutations of WFZP-D cause the SS phenotype, with the most severe effect when WFZP-D lesions are combined with a frameshift mutation in WFZP-A. We provide WFZP-based resources that may be useful for genetic manipulations with the aim of improving...

Russian Journal of Genetics, 2001

To estimate the possibility of plant genome mapping using human genome probes, the probes fluores... more To estimate the possibility of plant genome mapping using human genome probes, the probes fluorescent in situ hybridization (FISH) of human 18S–28S rDNA (clon 22F9 from the LA-13NCO1 library) was carried out on chromosomes of the spring barleyHordeum vulgareL. As a control, wheat rDNA probe (clon pTa71) was taken. Hybridization of the wheat DNA probe revealed two major labelling sites

Russian Journal of Developmental Biology, 2009

An improved modification of genomic in situ hybridization (GISH) was proposed. It allows clear an... more An improved modification of genomic in situ hybridization (GISH) was proposed. It allows clear and reproducible discrimination between closely related genomes of both tetraploid and hexaploid wheat species due to preannealing of labeled DNA probes and prehybridization of chromosomal samples with blocking DNA. The method was applied to analyze intergenomic translocations 6A:6B and 1A:6B identified in the IG46147 and IG116188 samples of tetraploid wheat Triticum dicoccoides by C-banding. The structure of the rearranged chromosomes was defined for two translocation variants, and the breakpoints were identified on the chromosome arms. Possible application of the developed GISH variant to study genome reorganizations during speciation of allopolyploid plants in evolution is discussed.

Russian Journal of Developmental Biology, 2011

Taking as starting point the erasure of the boundaries between criticism and fiction observed in ... more Taking as starting point the erasure of the boundaries between criticism and fiction observed in hybrid texts by Jorge Luis Borges and Roberto Bolaño, this paper investigates the incorporation of elements traditionally associated with literary sphere in critical texts that analyze the contemporary scene. From the dialogue between literature and theater, criticism and fiction, creative activity and theorist effort, we propose some critical-scenic experiments in order to promote short circuits between representation and reality, showing the false boundaries between scenic space and critical space.

Russian Journal of Genetics, 2009

Cluster analysis of the Triticum dicoccum chromosome passports by artificial neural networks and ... more Cluster analysis of the Triticum dicoccum chromosome passports by artificial neural networks and UPGMA divided the European T. dicoccum population into two groups, West European and Volga-Balkan. The West European T. dicoccum accessions displayed a predominance of the marker translocation T7A:5B (67% of the accessions), which was also found in a few accessions from other countries (Turkey, Iran, and northern Africa), and were similar in chromosome C-banding patterns. The Volga-Balkan T. dicoccum accessions differed in the C-banding patterns of some chromosomes from the West European accessions, thus probably originating from another founder population. It was assumed that the T. dicoccum accessions carrying the T7A:5B translocation had a common origin and that the wild T. dicoccum population of the Middle East (Syria and Lebanon) contributed to the origin of West European T. dicoccum.