Enzyme Structure Provides Clues (original) (raw)

Page last updated
September 30, 2002

Enzyme Structure Provides Clues To DNA Transcription Details

Before a cell can begin to divide or differentiate, the genetic information within the cell's DNA must be copied, or "transcribed," onto complementary strands of RNA. RNA polymerase II (pol II) is an enzyme that, by itself, can unwind the DNA double helix, synthesize RNA, and proofread the result. When combined with other molecules that regulate and control the transcription process, pol II is the key to successful interpretion of an organism's genetic code. However, the size, complexity, scarcity, and fragility of pol II complexes have made analysis of these macromolecules by x-ray crystallography a formidable challenge. A team of structural biologists has met this challenge using data obtained from both the Stanford Synchrotron Radiation Laboratory and the Macromolecular Crystallography Facility at the ALS. The resultant high-resolution model of a 10-subunit pol II complex suggests roles for each of the subunits and will allow researchers to begin unraveling the intricacies of DNA transcription and its role in gene expression.

In this work, the researchers studied the pol II enzyme from the yeast Saccharomyces cerevisiae, which is likely to be an excellent model for the human enzyme in light of its highly similar gene sequences. It is also the best-characterized form of the pol II enzyme, having been the subject of many biochemical and low-resolution structural studies in the past. To obtain a high-resolution structure, the research team drew on its considerable expertise in the preparation of protein crystals: two-dimensional crystals of pol II (minus two small subunits found to impede crystal growth) were used as seeds for growing three-dimensional crystals. These crystals, when produced in an inert atmosphere to prevent oxidation, enabled the collection of data to 3.5-angstrom resolution. The addition of a final soaking procedure to produce uniform crystals, combined with high-brightness x-ray sources, resulted in a resolution of 3.0 angstroms. Ribbon diagram of RNA polymerase II backbone model (top), and a color-coded schematic of the 10 subunits and their interactions (bottom).	Unraveling DNA Encoded into the double-helical strands of DNA, the human genome is the complete set of instructions required to make a human being. While the mapping of the human genome (roughly three billion components) is certainly a Herculean accomplishment, it is only the first step toward realizing the full potential of genomic medicine in the diagnosis, monitoring, and treatment of disease. Beyond knowing what the genetic blueprint says, scientists must understand how that blueprint gets interpreted, or "expressed" as an individual with unique traits. The pol II enzyme is the catalyst for a major step in this process. As a pol II molecule slides along a DNA molecule, it "unzips" the strands of the DNA double helix, synthesizes a complementary strand of RNA (which will carry the genetic information to where it is needed), and verifies that no mistakes have occurred. This process is regulated by transcription factors--separate molecules that bind to pol II and determine which genes are expressed, at what stage of development, and in which tissue. Done correctly, this process results in healthy cell growth and differentiation; otherwise, aberrations such as cancer can be the result. Thus, details of the structure of pol II, including information about its binding sites and how they interact with transcription factors, will provide valuable insight into the detailed mechanisms underlying the flow of genetic information from DNA to RNA to protein, which is necessary for life and health.
The current results bring into focus the somewhat fuzzy features previously observed in or inferred from earlier experiments. More importantly, the structural details suggest possible explanations for some of the unusual characteristics of this enzyme, which include a high processivity (the ability to synthesize very long strands of RNA) and the tendency to work in periodic spurts separated by pauses. While it is known that additional proteins (transcription factors) play a role in controlling the activity of pol II (for example, restarting after a pause), scientists have yet to understand how such proteins interact with pol II binding sites to perform their various functions. The pol II model reported here establishes the positions of the various subunits and provides detailed information about the DNA/RNA binding domains. The data reveal two main subunits (Rpb1 and Rpb2) separated by a deep cleft where DNA can enter the complex. At the end of the cleft is the active site, where the DNA can be unwound for a short distance (the "transcription bubble") and a DNA/RNA hybrid can be produced. Two prominent grooves lead away from the active site, either of which could accommodate the exiting RNA transcript. An opening below the active site may allow the entry of nucleotides (for manufacturing RNA) and transcription factors (for regulating the process). The same opening may provide room for the leading end of the RNA strand during "backtracking" maneuvers, which are important for proofreading and for traversing obstacles such as DNA damage. Other notable features that might help account for the great stability of this transcribing complex include a pair of "jaws" that appear to grip the DNA strands as they enter the complex and, closer to the active site, a clamp on the DNA that could possibly be locked in the closed position by the presence of RNA. The high-resolution pol II structure reported here is a landmark achievement, pulling together threads from numerous diverse research efforts into a cohesive whole. Further study should yield many new insights into the detailed mechanisms of pol II and its transcription factors. Construction of an atomic model is already well underway. Research conducted by P. Cramer, D.A. Bushnell, J. Fu, A.L. Gnatt, B. Maier-Davis, P.R. David, and R.D. Kornberg (Stanford University); N.E. Thompson and R.R. Burgess (University of Wisconsin-Madison); A.M. Edwards (University of Toronto). Research Funding: National Institutes of Health; Deutsche Forschungsgemeinschaft; American Cancer Society; U.S. Association of Medical Research Charities. Operation of the ALS is supported by the Office of Basic Energy Sciences (BES), U.S. Department of Energy. Publication about this research: P. Cramer, D.A. Bushnell, J. Fu, A.L. Gnatt, B. Maier-Davis, N.E. Thompson, R.R. Burgess, A.M. Edwards, P.R. David, R.D. Kornberg, "Architecture of RNA Polymerase II and Implications for the Transcription Mechanism," Science 288, 640 (2000). ALSNews Vol. 158, August 9, 2000

In this work, the researchers studied the pol II enzyme from the yeast Saccharomyces cerevisiae, which is likely to be an excellent model for the human enzyme in light of its highly similar gene sequences. It is also the best-characterized form of the pol II enzyme, having been the subject of many biochemical and low-resolution structural studies in the past. To obtain a high-resolution structure, the research team drew on its considerable expertise in the preparation of protein crystals: two-dimensional crystals of pol II (minus two small subunits found to impede crystal growth) were used as seeds for growing three-dimensional crystals. These crystals, when produced in an inert atmosphere to prevent oxidation, enabled the collection of data to 3.5-angstrom resolution. The addition of a final soaking procedure to produce uniform crystals, combined with high-brightness x-ray sources, resulted in a resolution of 3.0 angstroms. rna model

Ribbon diagram of RNA polymerase II backbone model (top), and a color-coded schematic of the 10 subunits and their interactions (bottom).

Unraveling DNA Encoded into the double-helical strands of DNA, the human genome is the complete set of instructions required to make a human being. While the mapping of the human genome (roughly three billion components) is certainly a Herculean accomplishment, it is only the first step toward realizing the full potential of genomic medicine in the diagnosis, monitoring, and treatment of disease. Beyond knowing what the genetic blueprint says, scientists must understand how that blueprint gets interpreted, or "expressed" as an individual with unique traits. The pol II enzyme is the catalyst for a major step in this process. As a pol II molecule slides along a DNA molecule, it "unzips" the strands of the DNA double helix, synthesizes a complementary strand of RNA (which will carry the genetic information to where it is needed), and verifies that no mistakes have occurred. This process is regulated by transcription factors--separate molecules that bind to pol II and determine which genes are expressed, at what stage of development, and in which tissue. Done correctly, this process results in healthy cell growth and differentiation; otherwise, aberrations such as cancer can be the result. Thus, details of the structure of pol II, including information about its binding sites and how they interact with transcription factors, will provide valuable insight into the detailed mechanisms underlying the flow of genetic information from DNA to RNA to protein, which is necessary for life and health.

The current results bring into focus the somewhat fuzzy features previously observed in or inferred from earlier experiments. More importantly, the structural details suggest possible explanations for some of the unusual characteristics of this enzyme, which include a high processivity (the ability to synthesize very long strands of RNA) and the tendency to work in periodic spurts separated by pauses. While it is known that additional proteins (transcription factors) play a role in controlling the activity of pol II (for example, restarting after a pause), scientists have yet to understand how such proteins interact with pol II binding sites to perform their various functions. The pol II model reported here establishes the positions of the various subunits and provides detailed information about the DNA/RNA binding domains. The data reveal two main subunits (Rpb1 and Rpb2) separated by a deep cleft where DNA can enter the complex. At the end of the cleft is the active site, where the DNA can be unwound for a short distance (the "transcription bubble") and a DNA/RNA hybrid can be produced. Two prominent grooves lead away from the active site, either of which could accommodate the exiting RNA transcript. An opening below the active site may allow the entry of nucleotides (for manufacturing RNA) and transcription factors (for regulating the process). The same opening may provide room for the leading end of the RNA strand during "backtracking" maneuvers, which are important for proofreading and for traversing obstacles such as DNA damage. Other notable features that might help account for the great stability of this transcribing complex include a pair of "jaws" that appear to grip the DNA strands as they enter the complex and, closer to the active site, a clamp on the DNA that could possibly be locked in the closed position by the presence of RNA. The high-resolution pol II structure reported here is a landmark achievement, pulling together threads from numerous diverse research efforts into a cohesive whole. Further study should yield many new insights into the detailed mechanisms of pol II and its transcription factors. Construction of an atomic model is already well underway. Research conducted by P. Cramer, D.A. Bushnell, J. Fu, A.L. Gnatt, B. Maier-Davis, P.R. David, and R.D. Kornberg (Stanford University); N.E. Thompson and R.R. Burgess (University of Wisconsin-Madison); A.M. Edwards (University of Toronto). Research Funding: National Institutes of Health; Deutsche Forschungsgemeinschaft; American Cancer Society; U.S. Association of Medical Research Charities. Operation of the ALS is supported by the Office of Basic Energy Sciences (BES), U.S. Department of Energy. Publication about this research: P. Cramer, D.A. Bushnell, J. Fu, A.L. Gnatt, B. Maier-Davis, N.E. Thompson, R.R. Burgess, A.M. Edwards, P.R. David, R.D. Kornberg, "Architecture of RNA Polymerase II and Implications for the Transcription Mechanism," Science 288, 640 (2000). ALSNews Vol. 158, August 9, 2000

Enzyme Structure Provides Clues (original) (raw)

Enzyme Structure Provides Clues To DNA Transcription Details

More ALS Science