Tan Tan | University of the West of Scotland (UWS) (original) (raw)

Papers by Tan Tan

Research paper thumbnail of Ökologische Untersuchungen über nitratreduzierende Bakterien im Wasser des Plußsees (Schleswig-Holstein)

Zeitschrift für allgemeine Mikrobiologie, 1973

Research paper thumbnail of Radioimmunotherapy of human colorectal carcinoma xenografts using 90Y-labeled monoclonal antibody CO17-1A prepared by two bifunctional chelate techniques

Cancer research, 1990

Monoclonal antibody COI7-1 A, which has specificity for colorectal and pancreatic carcinomas, was... more Monoclonal antibody COI7-1 A, which has specificity for colorectal and pancreatic carcinomas, was radiolabeled with the pure 0 emitter, '"'V by either the cyclic diethylenetriaminepentaacetic acid (DTPA) anhydride technique or by a site-specific bifunctional chelate technique ...

Research paper thumbnail of A regulatory effect of INMAP on centromere proteins: antisense INMAP induces CENP-B variation and centromeric halo

PloS one, 2014

CENP-B is a highly conserved protein that facilitates the assembly of specific centromere structu... more CENP-B is a highly conserved protein that facilitates the assembly of specific centromere structures both in interphase nuclei and on mitotic chromosomes. INMAP is a conserved protein that localizes at nucleus in interphase cells and at mitotic apparatus in mitotic cells. Our previous results showed that INMAP over-expression leads to spindle defects, mitotic arrest and formation of polycentrosomal and multinuclear cells, indicating that INMAP may modulate the function of (a) key protein(s) in mitotic apparatus. In this study, we demonstrate that INMAP interacts with CENP-B and promotes cleavage of the N-terminal DNA binding domain from CENP-B. The cleaved CENP-B cannot associate with centromeres and thus lose its centromere-related functions. Consistent with these results, CENP-B in INMAP knockdown cells becomes more diffused around kinetochores. Although INMAP knockdown cells do not exhibit gross defects in mitotic spindle formation, these cells go through mitosis, especially prop...

Research paper thumbnail of INMAP Overexpression Inhibits Cell Proliferation, Induces Genomic Instability and Functions through p53/p21 Pathways

PLOS ONE, 2015

INMAP is a spindle protein that plays essential role for mitosis, by ensuring spindle and centrom... more INMAP is a spindle protein that plays essential role for mitosis, by ensuring spindle and centromere integrality. The aim of this study was to investigate the relevant functions of INMAP for genomic stability and its functional pathway. We overexpressed INMAP in HeLa cells, resulting in growth inhibition in monolayer cell cultures, anchorage-independent growth in soft agar and xenograft growth in nude mice. In this system caused micronuclei (MNi) formation, chromosome distortion and γH2AX expression upregulation, suggesting DNA damage induction and genomic stability impairment. As a tumour biochemical marker, lactate dehydrogenase (LDH) isoenzymes were detected to evaluate cell metabolic activity, the results confirming that total activity of LDH, as well as that of its LDH5 isoform, is significantly decreased in INMAP-overexpressing HeLa cells. The levels of p53 and p21 were upregulated, and however, that of PCNA and Bcl-2, downregulated. Indirect immunofluorescence (IIF) and coimmunoprecipitation (CoIP) analyses revealed the interaction between INMAP and p21. These results suggest that INMAP might function through p53/p21 pathways.

Research paper thumbnail of Genetic diversity and differentiation of Sinocalycanthus chinensis populations revealed by chloroplast microsatellite (cpSSRs) markers

Biochemical Systematics and Ecology, 2012

To explore the endangered status of Sinocalycanthus chinensis, we examined the genetic diversity ... more To explore the endangered status of Sinocalycanthus chinensis, we examined the genetic diversity within and among S. chinensis populations using chloroplast microsatellite (cpSSRs) markers. Of the eight loci examined, five were monomorphic and three were polymorphic. A total of 13 different cpSSR alleles among 160 individuals was found. Genetic diversity at the population level was low (P ¼ 9.375%, A ¼ 1.0938, N e ¼ 1.0194, I ¼ 0.0263, h ¼ 0.0146), and genetic diversity at the species level was also relatively low (P ¼ 37.5%, A ¼ 1.6250, N e ¼ 1.1069, I ¼ 0.1072, h ¼ 0.0598). AMOVA analyses showed that variation existed mainly among populations (75.47%) rather than within populations (24.53%). Gene flow (N m ) estimated from G st was 0.1616. The test of isolation-by-distance via Mantel analysis showed significant association between genetic and geographical distances (r 2 ¼ 0.9530, p ¼ 0.048). Eight populations were clustered into two main groups: an eastern group and a western group.

Research paper thumbnail of Ökologische Untersuchungen über nitratreduzierende Bakterien im Wasser des Plußsees (Schleswig-Holstein)

Zeitschrift für allgemeine Mikrobiologie, 1973

Research paper thumbnail of Radioimmunotherapy of human colorectal carcinoma xenografts using 90Y-labeled monoclonal antibody CO17-1A prepared by two bifunctional chelate techniques

Cancer research, 1990

Monoclonal antibody COI7-1 A, which has specificity for colorectal and pancreatic carcinomas, was... more Monoclonal antibody COI7-1 A, which has specificity for colorectal and pancreatic carcinomas, was radiolabeled with the pure 0 emitter, '"'V by either the cyclic diethylenetriaminepentaacetic acid (DTPA) anhydride technique or by a site-specific bifunctional chelate technique ...

Research paper thumbnail of A regulatory effect of INMAP on centromere proteins: antisense INMAP induces CENP-B variation and centromeric halo

PloS one, 2014

CENP-B is a highly conserved protein that facilitates the assembly of specific centromere structu... more CENP-B is a highly conserved protein that facilitates the assembly of specific centromere structures both in interphase nuclei and on mitotic chromosomes. INMAP is a conserved protein that localizes at nucleus in interphase cells and at mitotic apparatus in mitotic cells. Our previous results showed that INMAP over-expression leads to spindle defects, mitotic arrest and formation of polycentrosomal and multinuclear cells, indicating that INMAP may modulate the function of (a) key protein(s) in mitotic apparatus. In this study, we demonstrate that INMAP interacts with CENP-B and promotes cleavage of the N-terminal DNA binding domain from CENP-B. The cleaved CENP-B cannot associate with centromeres and thus lose its centromere-related functions. Consistent with these results, CENP-B in INMAP knockdown cells becomes more diffused around kinetochores. Although INMAP knockdown cells do not exhibit gross defects in mitotic spindle formation, these cells go through mitosis, especially prop...

Research paper thumbnail of INMAP Overexpression Inhibits Cell Proliferation, Induces Genomic Instability and Functions through p53/p21 Pathways

PLOS ONE, 2015

INMAP is a spindle protein that plays essential role for mitosis, by ensuring spindle and centrom... more INMAP is a spindle protein that plays essential role for mitosis, by ensuring spindle and centromere integrality. The aim of this study was to investigate the relevant functions of INMAP for genomic stability and its functional pathway. We overexpressed INMAP in HeLa cells, resulting in growth inhibition in monolayer cell cultures, anchorage-independent growth in soft agar and xenograft growth in nude mice. In this system caused micronuclei (MNi) formation, chromosome distortion and γH2AX expression upregulation, suggesting DNA damage induction and genomic stability impairment. As a tumour biochemical marker, lactate dehydrogenase (LDH) isoenzymes were detected to evaluate cell metabolic activity, the results confirming that total activity of LDH, as well as that of its LDH5 isoform, is significantly decreased in INMAP-overexpressing HeLa cells. The levels of p53 and p21 were upregulated, and however, that of PCNA and Bcl-2, downregulated. Indirect immunofluorescence (IIF) and coimmunoprecipitation (CoIP) analyses revealed the interaction between INMAP and p21. These results suggest that INMAP might function through p53/p21 pathways.

Research paper thumbnail of Genetic diversity and differentiation of Sinocalycanthus chinensis populations revealed by chloroplast microsatellite (cpSSRs) markers

Biochemical Systematics and Ecology, 2012

To explore the endangered status of Sinocalycanthus chinensis, we examined the genetic diversity ... more To explore the endangered status of Sinocalycanthus chinensis, we examined the genetic diversity within and among S. chinensis populations using chloroplast microsatellite (cpSSRs) markers. Of the eight loci examined, five were monomorphic and three were polymorphic. A total of 13 different cpSSR alleles among 160 individuals was found. Genetic diversity at the population level was low (P ¼ 9.375%, A ¼ 1.0938, N e ¼ 1.0194, I ¼ 0.0263, h ¼ 0.0146), and genetic diversity at the species level was also relatively low (P ¼ 37.5%, A ¼ 1.6250, N e ¼ 1.1069, I ¼ 0.1072, h ¼ 0.0598). AMOVA analyses showed that variation existed mainly among populations (75.47%) rather than within populations (24.53%). Gene flow (N m ) estimated from G st was 0.1616. The test of isolation-by-distance via Mantel analysis showed significant association between genetic and geographical distances (r 2 ¼ 0.9530, p ¼ 0.048). Eight populations were clustered into two main groups: an eastern group and a western group.