Adeeba Anjum | University of Rajshahi (original) (raw)
Papers by Adeeba Anjum
The Pharma Innovation Journal, 2021
This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity and phospho molybdenum assay compared to the reference standard in a dose dependent manner. It was observed that the test samples contained considerable amount of bioactive compounds including total phenolic (ethyl acetate soluble fraction giving the highest 22.631 ± 0.085 GAE/gm of dried sample) and flavonoid (pet ether soluble fraction giving the highest 54.513 ± 1.500 CE/gm of dried sample) content. It can be concluded from the results that the crude methanolic extract along with it's four soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh are a good source of natural antioxidants.
International Research Journal Of Pharmacy
The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl... more The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl-(24)-ethylcholesta-5,22,25-triene, a steroidal glycoside isolated from the flowers of Alangium salviifolium Wang on Long Evan’s rat. After intra-peritoneal administration of the compound at a dose of 300 μg/rat/day for 14 consecutive days, no mortality or significant changes in body weight or behavior were observed. The blood samples of the rats were examined for hematological and biochemical parameters which were statistically insignificant when compared to that of the control group. All the vital organs showed normal histopathological architecture (heart, lungs, liver and kidney) in comparison to the control group. This preliminary investigation demonstrate that the compound is safe at dose of 300 μg/rat/day for 14 consecutive days. But acute, sub-chronic and chronic toxicity evaluations as well as clinical trials need to be done.
Journal of Pharmacognosy and Phytochemistry, 2021
In the present study, antioxidant potential of the crude methanol extract along with its n-hexane... more In the present study, antioxidant potential of the crude methanol extract along with its n-hexane, chloroform, ethyl acetate and aqueous soluble fractions of the roots of Coffea benghalensis growing in Bangladesh was assessed by using DPPH scavenging assay, reducing power, hydroxyl radical scavenging assay and total antioxidant capacity. In DPPH scavenging assay the IC50 value of the ethyl acetate soluble fraction was found to be 47.56 µg/ml while the reference standard ascorbic acid was 1.42 µg/ml. The reducing power showed good linear relations between the standard ascorbic acid and all the test samples. Higher hydroxyl radical activity was shown by the n-hexane soluble fraction with IC50 value 58.58 µg/ml whereas the standard catechin was 89.61 µg/ml. The extracts showed significant activities in total the antioxidant assay compared to the reference standard in a dose dependent manner. Also total phenolic and flavonoid content was evaluated which exhibited remarkable values.
The Journal of Phytopharmacology, 2021
This study was designed to evaluate the in vitro antibacterial and in vivo cytotoxic activity of ... more This study was designed to evaluate the in vitro antibacterial and in vivo cytotoxic activity of the roots of Bangladeshi Coffea benghalensis B Heyne ex Schult. (Family: Rubiaceae), locally known as bonnyo kofee native to the regions of Sylhet and Chittagong in Bangladesh. The finely crushed coarse powder was extracted with methanol and solvent-solvent partitioning was done to obtain n-hexane, chloroform, ethyl acetate and aqueous soluble fractions. The crude methanol extract along with it’s four soluble fractions were then evaluated for their in vitro antibacterial and in vivo cytotoxic activities. The disc diffusion method was used to evaluate the antibacterial activity at a concentration of 200 and 400 μg/disc. All the soluble fractions and the crude methanolic extract did not demonstrate any zone of inhibition. In case of brine shrimp lethality bioassay, all the test samples exhibited cytotoxic activity against brine shrimp nauplii having remarkable LC50 and LC90 values. The n-h...
Bangladesh Pharmaceutical Journal, 2022
The current study was conducted to assess the antioxidant, antimicrobial, cytotoxicity and thromb... more The current study was conducted to assess the antioxidant, antimicrobial, cytotoxicity and thrombolytic potential of the leaves and stem bark of Erythrina fusca Lour grown in Bangladesh. For the assessment of antioxidant properties, DPPH scavenging and phosphomolybdenum assays were used. In the DPPH free radical scavenging assay, the chloroform soluble fraction of a methanol extract of leaves of E. fusca exhibited the highest free radical scavenging activity with an IC50 value of 5.5 ± 0.60 μg/ml, while the reference standard (ascorbic acid) showed an IC50 value of 5.8 ± 0.18 μg/ml. All the test samples and the standard BHT demonstrated good linear relationship in the phosphomolybdenum assay. It was observed that the test samples contained considerable number of bioactive compounds including total phenolics in the aqueous soluble fraction of the leaves giving the highest 94.02 ± 1.004 GAE/100g of dried sample. All test samples exhibited moderate antimicrobial activity against 13 Gra...
Journal of Medicinal Plants, 2021
Background: Erythrina fusca Lour. (fam. Fabaceae) is a flowering tree, found extensively in tropi... more Background: Erythrina fusca Lour. (fam. Fabaceae) is a flowering tree, found extensively in tropical and subtropical Asian countries, and is known for its use in traditional medicine for the treatment of various human ailments, for example, fever, liver complications, infections, and headaches. Objective: To carry out phytochemical study, and antimicrobial and free-radical scavenging activity evaluation of E. fusca. Methods: Ground stem bark of this plant was extracted by maceration with methanol, partitioned with various organic solvents, and compounds were isolated by chromatographic means. Structures of isolated compounds were confirmed by spectroscopic analyses. The antibacterial activity was assessed by the disc diffusion method, and the free-radical scavenging activity was determined by DPPH assay. Results: The carbon tetrachloride soluble fraction of the methanol extract of E. fusca afforded shinpterocarpin (1), lupinifolin (2), 3,9-dihydroxy-4-(3,3-dimethylallyl) [6aR,11aR]-pterocarpan (3) and β-sitosterol (4). Compounds 1-3 showed considerable antimicrobial activity against five Gram-positive and eight Gram-negative bacterial and three fungal strains tested in this study. Compound 1 exhibited the highest zone of inhibition of 19.4 mm against Bacillus subtilis. Additionally, compounds showed freeradical scavenging effects in DPPH assay with the IC50 values of 8.8, 7.7 and 7.9 µg/mL for compound 1, 2 and 3, respectively. However, they displayed some general toxicity in BSL assay. Conclusion: The isolation of bioactive compounds 1-3 supports some traditional medicinal uses of this plant. However, general toxicities found in the BSL assay might raise concerns regarding its safety, while offering a new avenue of future investigation on cytotoxicity of these compounds against human cancer cell lines.
The Pharma Innovation Journal, 2021
This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity ...
International Research Journal Of Pharmacy, 2021
The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl... more The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl-(24)-ethylcholesta-5,22,25-triene, a steroidal glycoside isolated from the flowers of Alangium salviifolium Wang on Long Evan’s rat. After intra-peritoneal administration of the compound at a dose of 300 μg/rat/day for 14 consecutive days, no mortality or significant changes in body weight or behavior were observed. The blood samples of the rats were examined for hematological and biochemical parameters which were statistically insignificant when compared to that of the control group. All the vital organs showed normal histopathological architecture (heart, lungs, liver and kidney) in comparison to the control group. This preliminary investigation demonstrate that the compound is safe at dose of 300 μg/rat/day for 14 consecutive days. But acute, sub-chronic and chronic toxicity evaluations as well as clinical trials need to be done.
Dhaka University Journal of Pharmaceutical Sciences
Bridelia tomentosa Blume is a small evergreen tree which has enormous folklore applications in th... more Bridelia tomentosa Blume is a small evergreen tree which has enormous folklore applications in the treatment of colic, traumatic injury, epidemic influenza and neurasthenia. The main purpose of this study was to isolate bioactive compounds from the stem bark of B. tomentosa growing in Bangladesh. Extensive chromatographic separation and purification of the methanolic extract of stem bark of B. tomentosa led to the isolation of four compounds. The purified constituents were identified as friedoolean-5(6),14(15)-dien-3-one (1), β-taraxerol (2), D4- stigmasterone (3) and lupeol (4) by extensive analysis of NMR spectroscopic data. While compound 1 is appeared to be new, the other compounds (2-4) have been isolated for the first time from this plant. β-taraxerol (2) demonstrated significant cytotoxic activity against the brine shrimp Artemia salina and moderate to strong antimicrobial efficacy with the highest inhibitory potential against Salmonella Typhi (zone of inhibition = 21.3 mm) a...
The Pharma Innovation Journal, 2021
This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity ...
International research journal of pharmacy, 2011
The organic soluble extractives of three Bridelia species, B. verrucosa, B. stipularis and B. tom... more The organic soluble extractives of three Bridelia species, B. verrucosa, B. stipularis and B. tomentosa growing in Bangladesh were subjected to screening for antibacterial, antifungal and cytotoxic activities. All extractives showed moderate to strong antimicrobial activity against 13 Gram positive and Gram negative bacterial strains and three fungi where the stem bark of B. tomentosa demonstrated highest inhibition of growth with zone of inhibition of 23.2 mm againstBacillus cereus and 17.5 mm against Candida albicans. The crude extractives of all three plants of Bridelia species exhibited cytotoxic activity against brine shrimp nauplii having significant LC50 and LC90.
Dhaka University Journal of Pharmaceutical Sciences
Fractionation and purification of methanolic extract of stem bark of the Bridelia verrucosa growi... more Fractionation and purification of methanolic extract of stem bark of the Bridelia verrucosa growing in Bangladesh afforded glochidonol (1), brassicasterol (2), friedelin (3), 5β-24S-ethylchoestane-3β-ol (4), stigmasterol (5), ketooleanane (6), 5-5'-dihydroxy sesamine (7) and pinoresinol (8). Compounds 1, 2, 3, 4, 6 and 8 are the first report of their isolation from the plant while compound 7 appear to be a new isolate from natural source. The isolated compounds exhibited cytotoxic activity against brine shrimp nauplii having significant LC 50 and LC 90 values and moderate to strong antimicrobial activity against 13 Gram positive and Gram negative bacterial strains and 3 fungi. Compound 8 showed highest inhibition of growth of microorganisms with zone of inhibition of 16.7 mm against Shigella dysenteriae. Compounds 1-4 and 6-8 also revealed moderate free radical scavenging activity, in DPPH assay.
Dhaka University Journal of Pharmaceutical Sciences, 2017
Fractionation and purification of stem bark extract of Bridelia stipularis growing in Bangladesh ... more Fractionation and purification of stem bark extract of Bridelia stipularis growing in Bangladesh afforded glut-5(6)-en-3-one (1), glut-5(6)-en-3α-ol (2), and (22E)-7-hydroxy-28-methylcholesta-4,22-dien-3-one (3). Compound 3 appears to be new, while compounds 1 and 2 have never been reported from this plant. The isolated compounds isolated exhibited cytotoxic activity against brine shrimp nauplii having significant LC 50 and LC 90 and moderate to strong antimicrobial activity against 13 Gram positive and Gram negative bacterial strains and 3 fungi. Here, compound 1 demonstrated highest inhibition of growth of microorganisms with zone of inhibition of 22.7 mm against Escherichia coli and compound 2 displayed zone of inhibition of 20.8 mm against Candida albicans. Compounds 1-2 also revealed moderate free radical scavenging activity in the DPPH method.
Journal of Scientific Research, 2013
The organic soluble extractives of three Bridelia species, B. verrucosa, B. stipularis and B. tom... more The organic soluble extractives of three Bridelia species, B. verrucosa, B. stipularis and B. tomentosa growing in Bangladesh were subjected to screening for free radical scavenging activity, total antioxidant capacity and total phenolic content. All of the methanol extracts of the these plants and their kupchan fractions showed moderate to strong free radical scavenging activity, the total antioxidant capacity and total phenolic content, of which the methanol extract of the leaf of B. verrucosa revealed highest activity having IC 50 value of 6.35 µg/ml. All the extractives of three plants were also studied for their thrombolytic potential. Among the three plants the carbon tetrachloride soluble fraction and methanol extract of leaf and aqueous soluble fraction of bark of B. tomentosa, methanol extract of bark of B. stipularis and carbon tetrachloride soluble fraction of leaf of B. verrucosa exhibited highest thrombolytic activity with clot lysis value of 41.46%, 34.85%, 37.04%, 36.45% and 33.72%, respectively. Standard streptokinase was used as positive control which exhibited 61.50% lysis of clot while the negative control water revealed 2.56% lysis of clot.
Nigerian Journal of Natural Products and Medicine, 2002
Abstract The in vitro antibacterial activity of the methanol extracts of leaf, root of Rauvolfia ... more Abstract The in vitro antibacterial activity of the methanol extracts of leaf, root of Rauvolfia serpentina and its tissue culture callus, root, leaf were investigated. The extract showed a good antibacterial activity against gram negative organisms, which may be due to the ...
Fitoterapia, 2002
1-Methyl-1H-pyrimidine-2,4-dione and 3-O-beta-D-glucopyranosyl-(24beta)-ethylcholesta-5,22,25-tri... more 1-Methyl-1H-pyrimidine-2,4-dione and 3-O-beta-D-glucopyranosyl-(24beta)-ethylcholesta-5,22,25-triene, isolated from the flowers of Alangium salviifolium, showed remarkable antibacterial activities against a number of Gram-positive and Gram-negative bacterial species.
This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity and phospho molybdenum assay compared to the reference standard in a dose dependent manner. It was observed that the test samples contained considerable amount of bioactive compounds including total phenolic (ethyl acetate soluble fraction giving the highest 22.631 ± 0.085 GAE/gm of dried sample) and flavonoid (pet ether soluble fraction giving the highest 54.513 ± 1.500 CE/gm of dried sample) content. It can be concluded from the results that the crude methanolic extract along with it's four soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh are a good source of natural antioxidants.
This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity and phospho molybdenum assay compared to the reference standard in a dose dependent manner. It was observed that the test samples contained considerable amount of bioactive compounds including total phenolic (ethyl acetate soluble fraction giving the highest 22.631 ± 0.085 GAE/gm of dried sample) and flavonoid (pet ether soluble fraction giving the highest 54.513 ± 1.500 CE/gm of dried sample) content. It can be concluded from the results that the crude methanolic extract along with it's four soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh are a good source of natural antioxidants.
The Pharma Innovation Journal, 2021
This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity and phospho molybdenum assay compared to the reference standard in a dose dependent manner. It was observed that the test samples contained considerable amount of bioactive compounds including total phenolic (ethyl acetate soluble fraction giving the highest 22.631 ± 0.085 GAE/gm of dried sample) and flavonoid (pet ether soluble fraction giving the highest 54.513 ± 1.500 CE/gm of dried sample) content. It can be concluded from the results that the crude methanolic extract along with it's four soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh are a good source of natural antioxidants.
International Research Journal Of Pharmacy
The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl... more The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl-(24)-ethylcholesta-5,22,25-triene, a steroidal glycoside isolated from the flowers of Alangium salviifolium Wang on Long Evan’s rat. After intra-peritoneal administration of the compound at a dose of 300 μg/rat/day for 14 consecutive days, no mortality or significant changes in body weight or behavior were observed. The blood samples of the rats were examined for hematological and biochemical parameters which were statistically insignificant when compared to that of the control group. All the vital organs showed normal histopathological architecture (heart, lungs, liver and kidney) in comparison to the control group. This preliminary investigation demonstrate that the compound is safe at dose of 300 μg/rat/day for 14 consecutive days. But acute, sub-chronic and chronic toxicity evaluations as well as clinical trials need to be done.
Journal of Pharmacognosy and Phytochemistry, 2021
In the present study, antioxidant potential of the crude methanol extract along with its n-hexane... more In the present study, antioxidant potential of the crude methanol extract along with its n-hexane, chloroform, ethyl acetate and aqueous soluble fractions of the roots of Coffea benghalensis growing in Bangladesh was assessed by using DPPH scavenging assay, reducing power, hydroxyl radical scavenging assay and total antioxidant capacity. In DPPH scavenging assay the IC50 value of the ethyl acetate soluble fraction was found to be 47.56 µg/ml while the reference standard ascorbic acid was 1.42 µg/ml. The reducing power showed good linear relations between the standard ascorbic acid and all the test samples. Higher hydroxyl radical activity was shown by the n-hexane soluble fraction with IC50 value 58.58 µg/ml whereas the standard catechin was 89.61 µg/ml. The extracts showed significant activities in total the antioxidant assay compared to the reference standard in a dose dependent manner. Also total phenolic and flavonoid content was evaluated which exhibited remarkable values.
The Journal of Phytopharmacology, 2021
This study was designed to evaluate the in vitro antibacterial and in vivo cytotoxic activity of ... more This study was designed to evaluate the in vitro antibacterial and in vivo cytotoxic activity of the roots of Bangladeshi Coffea benghalensis B Heyne ex Schult. (Family: Rubiaceae), locally known as bonnyo kofee native to the regions of Sylhet and Chittagong in Bangladesh. The finely crushed coarse powder was extracted with methanol and solvent-solvent partitioning was done to obtain n-hexane, chloroform, ethyl acetate and aqueous soluble fractions. The crude methanol extract along with it’s four soluble fractions were then evaluated for their in vitro antibacterial and in vivo cytotoxic activities. The disc diffusion method was used to evaluate the antibacterial activity at a concentration of 200 and 400 μg/disc. All the soluble fractions and the crude methanolic extract did not demonstrate any zone of inhibition. In case of brine shrimp lethality bioassay, all the test samples exhibited cytotoxic activity against brine shrimp nauplii having remarkable LC50 and LC90 values. The n-h...
Bangladesh Pharmaceutical Journal, 2022
The current study was conducted to assess the antioxidant, antimicrobial, cytotoxicity and thromb... more The current study was conducted to assess the antioxidant, antimicrobial, cytotoxicity and thrombolytic potential of the leaves and stem bark of Erythrina fusca Lour grown in Bangladesh. For the assessment of antioxidant properties, DPPH scavenging and phosphomolybdenum assays were used. In the DPPH free radical scavenging assay, the chloroform soluble fraction of a methanol extract of leaves of E. fusca exhibited the highest free radical scavenging activity with an IC50 value of 5.5 ± 0.60 μg/ml, while the reference standard (ascorbic acid) showed an IC50 value of 5.8 ± 0.18 μg/ml. All the test samples and the standard BHT demonstrated good linear relationship in the phosphomolybdenum assay. It was observed that the test samples contained considerable number of bioactive compounds including total phenolics in the aqueous soluble fraction of the leaves giving the highest 94.02 ± 1.004 GAE/100g of dried sample. All test samples exhibited moderate antimicrobial activity against 13 Gra...
Journal of Medicinal Plants, 2021
Background: Erythrina fusca Lour. (fam. Fabaceae) is a flowering tree, found extensively in tropi... more Background: Erythrina fusca Lour. (fam. Fabaceae) is a flowering tree, found extensively in tropical and subtropical Asian countries, and is known for its use in traditional medicine for the treatment of various human ailments, for example, fever, liver complications, infections, and headaches. Objective: To carry out phytochemical study, and antimicrobial and free-radical scavenging activity evaluation of E. fusca. Methods: Ground stem bark of this plant was extracted by maceration with methanol, partitioned with various organic solvents, and compounds were isolated by chromatographic means. Structures of isolated compounds were confirmed by spectroscopic analyses. The antibacterial activity was assessed by the disc diffusion method, and the free-radical scavenging activity was determined by DPPH assay. Results: The carbon tetrachloride soluble fraction of the methanol extract of E. fusca afforded shinpterocarpin (1), lupinifolin (2), 3,9-dihydroxy-4-(3,3-dimethylallyl) [6aR,11aR]-pterocarpan (3) and β-sitosterol (4). Compounds 1-3 showed considerable antimicrobial activity against five Gram-positive and eight Gram-negative bacterial and three fungal strains tested in this study. Compound 1 exhibited the highest zone of inhibition of 19.4 mm against Bacillus subtilis. Additionally, compounds showed freeradical scavenging effects in DPPH assay with the IC50 values of 8.8, 7.7 and 7.9 µg/mL for compound 1, 2 and 3, respectively. However, they displayed some general toxicity in BSL assay. Conclusion: The isolation of bioactive compounds 1-3 supports some traditional medicinal uses of this plant. However, general toxicities found in the BSL assay might raise concerns regarding its safety, while offering a new avenue of future investigation on cytotoxicity of these compounds against human cancer cell lines.
The Pharma Innovation Journal, 2021
This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity ...
International Research Journal Of Pharmacy, 2021
The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl... more The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl-(24)-ethylcholesta-5,22,25-triene, a steroidal glycoside isolated from the flowers of Alangium salviifolium Wang on Long Evan’s rat. After intra-peritoneal administration of the compound at a dose of 300 μg/rat/day for 14 consecutive days, no mortality or significant changes in body weight or behavior were observed. The blood samples of the rats were examined for hematological and biochemical parameters which were statistically insignificant when compared to that of the control group. All the vital organs showed normal histopathological architecture (heart, lungs, liver and kidney) in comparison to the control group. This preliminary investigation demonstrate that the compound is safe at dose of 300 μg/rat/day for 14 consecutive days. But acute, sub-chronic and chronic toxicity evaluations as well as clinical trials need to be done.
Dhaka University Journal of Pharmaceutical Sciences
Bridelia tomentosa Blume is a small evergreen tree which has enormous folklore applications in th... more Bridelia tomentosa Blume is a small evergreen tree which has enormous folklore applications in the treatment of colic, traumatic injury, epidemic influenza and neurasthenia. The main purpose of this study was to isolate bioactive compounds from the stem bark of B. tomentosa growing in Bangladesh. Extensive chromatographic separation and purification of the methanolic extract of stem bark of B. tomentosa led to the isolation of four compounds. The purified constituents were identified as friedoolean-5(6),14(15)-dien-3-one (1), β-taraxerol (2), D4- stigmasterone (3) and lupeol (4) by extensive analysis of NMR spectroscopic data. While compound 1 is appeared to be new, the other compounds (2-4) have been isolated for the first time from this plant. β-taraxerol (2) demonstrated significant cytotoxic activity against the brine shrimp Artemia salina and moderate to strong antimicrobial efficacy with the highest inhibitory potential against Salmonella Typhi (zone of inhibition = 21.3 mm) a...
The Pharma Innovation Journal, 2021
This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity ...
International research journal of pharmacy, 2011
The organic soluble extractives of three Bridelia species, B. verrucosa, B. stipularis and B. tom... more The organic soluble extractives of three Bridelia species, B. verrucosa, B. stipularis and B. tomentosa growing in Bangladesh were subjected to screening for antibacterial, antifungal and cytotoxic activities. All extractives showed moderate to strong antimicrobial activity against 13 Gram positive and Gram negative bacterial strains and three fungi where the stem bark of B. tomentosa demonstrated highest inhibition of growth with zone of inhibition of 23.2 mm againstBacillus cereus and 17.5 mm against Candida albicans. The crude extractives of all three plants of Bridelia species exhibited cytotoxic activity against brine shrimp nauplii having significant LC50 and LC90.
Dhaka University Journal of Pharmaceutical Sciences
Fractionation and purification of methanolic extract of stem bark of the Bridelia verrucosa growi... more Fractionation and purification of methanolic extract of stem bark of the Bridelia verrucosa growing in Bangladesh afforded glochidonol (1), brassicasterol (2), friedelin (3), 5β-24S-ethylchoestane-3β-ol (4), stigmasterol (5), ketooleanane (6), 5-5'-dihydroxy sesamine (7) and pinoresinol (8). Compounds 1, 2, 3, 4, 6 and 8 are the first report of their isolation from the plant while compound 7 appear to be a new isolate from natural source. The isolated compounds exhibited cytotoxic activity against brine shrimp nauplii having significant LC 50 and LC 90 values and moderate to strong antimicrobial activity against 13 Gram positive and Gram negative bacterial strains and 3 fungi. Compound 8 showed highest inhibition of growth of microorganisms with zone of inhibition of 16.7 mm against Shigella dysenteriae. Compounds 1-4 and 6-8 also revealed moderate free radical scavenging activity, in DPPH assay.
Dhaka University Journal of Pharmaceutical Sciences, 2017
Fractionation and purification of stem bark extract of Bridelia stipularis growing in Bangladesh ... more Fractionation and purification of stem bark extract of Bridelia stipularis growing in Bangladesh afforded glut-5(6)-en-3-one (1), glut-5(6)-en-3α-ol (2), and (22E)-7-hydroxy-28-methylcholesta-4,22-dien-3-one (3). Compound 3 appears to be new, while compounds 1 and 2 have never been reported from this plant. The isolated compounds isolated exhibited cytotoxic activity against brine shrimp nauplii having significant LC 50 and LC 90 and moderate to strong antimicrobial activity against 13 Gram positive and Gram negative bacterial strains and 3 fungi. Here, compound 1 demonstrated highest inhibition of growth of microorganisms with zone of inhibition of 22.7 mm against Escherichia coli and compound 2 displayed zone of inhibition of 20.8 mm against Candida albicans. Compounds 1-2 also revealed moderate free radical scavenging activity in the DPPH method.
Journal of Scientific Research, 2013
The organic soluble extractives of three Bridelia species, B. verrucosa, B. stipularis and B. tom... more The organic soluble extractives of three Bridelia species, B. verrucosa, B. stipularis and B. tomentosa growing in Bangladesh were subjected to screening for free radical scavenging activity, total antioxidant capacity and total phenolic content. All of the methanol extracts of the these plants and their kupchan fractions showed moderate to strong free radical scavenging activity, the total antioxidant capacity and total phenolic content, of which the methanol extract of the leaf of B. verrucosa revealed highest activity having IC 50 value of 6.35 µg/ml. All the extractives of three plants were also studied for their thrombolytic potential. Among the three plants the carbon tetrachloride soluble fraction and methanol extract of leaf and aqueous soluble fraction of bark of B. tomentosa, methanol extract of bark of B. stipularis and carbon tetrachloride soluble fraction of leaf of B. verrucosa exhibited highest thrombolytic activity with clot lysis value of 41.46%, 34.85%, 37.04%, 36.45% and 33.72%, respectively. Standard streptokinase was used as positive control which exhibited 61.50% lysis of clot while the negative control water revealed 2.56% lysis of clot.
Nigerian Journal of Natural Products and Medicine, 2002
Abstract The in vitro antibacterial activity of the methanol extracts of leaf, root of Rauvolfia ... more Abstract The in vitro antibacterial activity of the methanol extracts of leaf, root of Rauvolfia serpentina and its tissue culture callus, root, leaf were investigated. The extract showed a good antibacterial activity against gram negative organisms, which may be due to the ...
Fitoterapia, 2002
1-Methyl-1H-pyrimidine-2,4-dione and 3-O-beta-D-glucopyranosyl-(24beta)-ethylcholesta-5,22,25-tri... more 1-Methyl-1H-pyrimidine-2,4-dione and 3-O-beta-D-glucopyranosyl-(24beta)-ethylcholesta-5,22,25-triene, isolated from the flowers of Alangium salviifolium, showed remarkable antibacterial activities against a number of Gram-positive and Gram-negative bacterial species.
This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity and phospho molybdenum assay compared to the reference standard in a dose dependent manner. It was observed that the test samples contained considerable amount of bioactive compounds including total phenolic (ethyl acetate soluble fraction giving the highest 22.631 ± 0.085 GAE/gm of dried sample) and flavonoid (pet ether soluble fraction giving the highest 54.513 ± 1.500 CE/gm of dried sample) content. It can be concluded from the results that the crude methanolic extract along with it's four soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh are a good source of natural antioxidants.
This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity and phospho molybdenum assay compared to the reference standard in a dose dependent manner. It was observed that the test samples contained considerable amount of bioactive compounds including total phenolic (ethyl acetate soluble fraction giving the highest 22.631 ± 0.085 GAE/gm of dried sample) and flavonoid (pet ether soluble fraction giving the highest 54.513 ± 1.500 CE/gm of dried sample) content. It can be concluded from the results that the crude methanolic extract along with it's four soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh are a good source of natural antioxidants.