Adeeba Anjum | University of Rajshahi (original) (raw)

Papers by Adeeba Anjum

Research paper thumbnail of In vitro evaluation of antioxidant potentiality and estimation of total phenolic and flavonoid content of the whole stem of Coffea benghalensis B. Heyne ex Schult

The Pharma Innovation Journal, 2021

Research paper thumbnail of Sub-Acute Toxicity of a Steroidal Glycoside from the Flowers of Alangium Salviifolium Wang

International Research Journal Of Pharmacy

The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl... more The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl-(24)-ethylcholesta-5,22,25-triene, a steroidal glycoside isolated from the flowers of Alangium salviifolium Wang on Long Evan’s rat. After intra-peritoneal administration of the compound at a dose of 300 μg/rat/day for 14 consecutive days, no mortality or significant changes in body weight or behavior were observed. The blood samples of the rats were examined for hematological and biochemical parameters which were statistically insignificant when compared to that of the control group. All the vital organs showed normal histopathological architecture (heart, lungs, liver and kidney) in comparison to the control group. This preliminary investigation demonstrate that the compound is safe at dose of 300 μg/rat/day for 14 consecutive days. But acute, sub-chronic and chronic toxicity evaluations as well as clinical trials need to be done.

Research paper thumbnail of In vitro antioxidant activities of the roots of Coffea benghalensis B Heyne ex Schult. Growing in Bangladesh

Journal of Pharmacognosy and Phytochemistry, 2021

Research paper thumbnail of Evaluation of in vitro antibacterial and in vivo cytotoxic activities of Bangladeshi Coffea benghalensis B Heyne ex Schult. roots

The Journal of Phytopharmacology, 2021

This study was designed to evaluate the in vitro antibacterial and in vivo cytotoxic activity of ... more This study was designed to evaluate the in vitro antibacterial and in vivo cytotoxic activity of the roots of Bangladeshi Coffea benghalensis B Heyne ex Schult. (Family: Rubiaceae), locally known as bonnyo kofee native to the regions of Sylhet and Chittagong in Bangladesh. The finely crushed coarse powder was extracted with methanol and solvent-solvent partitioning was done to obtain n-hexane, chloroform, ethyl acetate and aqueous soluble fractions. The crude methanol extract along with it’s four soluble fractions were then evaluated for their in vitro antibacterial and in vivo cytotoxic activities. The disc diffusion method was used to evaluate the antibacterial activity at a concentration of 200 and 400 μg/disc. All the soluble fractions and the crude methanolic extract did not demonstrate any zone of inhibition. In case of brine shrimp lethality bioassay, all the test samples exhibited cytotoxic activity against brine shrimp nauplii having remarkable LC50 and LC90 values. The n-h...

Research paper thumbnail of Assessment of Antioxidant, Antimicrobial, Cytotoxicity and Thrombolytic Potential of Erythrina fusca Lour Grown in Bangladesh

Bangladesh Pharmaceutical Journal, 2022

The current study was conducted to assess the antioxidant, antimicrobial, cytotoxicity and thromb... more The current study was conducted to assess the antioxidant, antimicrobial, cytotoxicity and thrombolytic potential of the leaves and stem bark of Erythrina fusca Lour grown in Bangladesh. For the assessment of antioxidant properties, DPPH scavenging and phosphomolybdenum assays were used. In the DPPH free radical scavenging assay, the chloroform soluble fraction of a methanol extract of leaves of E. fusca exhibited the highest free radical scavenging activity with an IC50 value of 5.5 ± 0.60 μg/ml, while the reference standard (ascorbic acid) showed an IC50 value of 5.8 ± 0.18 μg/ml. All the test samples and the standard BHT demonstrated good linear relationship in the phosphomolybdenum assay. It was observed that the test samples contained considerable number of bioactive compounds including total phenolics in the aqueous soluble fraction of the leaves giving the highest 94.02 ± 1.004 GAE/100g of dried sample. All test samples exhibited moderate antimicrobial activity against 13 Gra...

Research paper thumbnail of Flavonoid, pterocarpans and steroid from Erythrina fusca Lour. growing in Bangladesh: isolation, and antimicrobial and free-radical scavenging activity

Journal of Medicinal Plants, 2021

Research paper thumbnail of In vitro evaluation of antioxidant potentiality and estimation of total phenolic and flavonoid content of the whole stem of Coffea benghalensis B. Heyne ex Schult

The Pharma Innovation Journal, 2021

This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity ...

Research paper thumbnail of Sub-Acute Toxicity of a Steroidal Glycoside from the Flowers of Alangium Salviifolium Wang

International Research Journal Of Pharmacy, 2021

The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl... more The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl-(24)-ethylcholesta-5,22,25-triene, a steroidal glycoside isolated from the flowers of Alangium salviifolium Wang on Long Evan’s rat. After intra-peritoneal administration of the compound at a dose of 300 μg/rat/day for 14 consecutive days, no mortality or significant changes in body weight or behavior were observed. The blood samples of the rats were examined for hematological and biochemical parameters which were statistically insignificant when compared to that of the control group. All the vital organs showed normal histopathological architecture (heart, lungs, liver and kidney) in comparison to the control group. This preliminary investigation demonstrate that the compound is safe at dose of 300 μg/rat/day for 14 consecutive days. But acute, sub-chronic and chronic toxicity evaluations as well as clinical trials need to be done.

Research paper thumbnail of Phytochemical and Biological Investigation of Bridelia tomentosa Blume Growing in Bangladesh

Dhaka University Journal of Pharmaceutical Sciences

Bridelia tomentosa Blume is a small evergreen tree which has enormous folklore applications in th... more Bridelia tomentosa Blume is a small evergreen tree which has enormous folklore applications in the treatment of colic, traumatic injury, epidemic influenza and neurasthenia. The main purpose of this study was to isolate bioactive compounds from the stem bark of B. tomentosa growing in Bangladesh. Extensive chromatographic separation and purification of the methanolic extract of stem bark of B. tomentosa led to the isolation of four compounds. The purified constituents were identified as friedoolean-5(6),14(15)-dien-3-one (1), β-taraxerol (2), D4- stigmasterone (3) and lupeol (4) by extensive analysis of NMR spectroscopic data. While compound 1 is appeared to be new, the other compounds (2-4) have been isolated for the first time from this plant. β-taraxerol (2) demonstrated significant cytotoxic activity against the brine shrimp Artemia salina and moderate to strong antimicrobial efficacy with the highest inhibitory potential against Salmonella Typhi (zone of inhibition = 21.3 mm) a...

Research paper thumbnail of In vitro evaluation of antioxidant potentiality and estimation of total phenolic and flavonoid content of the whole stem of Coffea benghalensis B. Heyne ex Schult

The Pharma Innovation Journal, 2021

This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity ...

Research paper thumbnail of In vitro antibacterial, antifungal and cytotoxic activity of three Bangladeshi Bridelia species

International research journal of pharmacy, 2011

The organic soluble extractives of three Bridelia species, B. verrucosa, B. stipularis and B. tom... more The organic soluble extractives of three Bridelia species, B. verrucosa, B. stipularis and B. tomentosa growing in Bangladesh were subjected to screening for antibacterial, antifungal and cytotoxic activities. All extractives showed moderate to strong antimicrobial activity against 13 Gram positive and Gram negative bacterial strains and three fungi where the stem bark of B. tomentosa demonstrated highest inhibition of growth with zone of inhibition of 23.2 mm againstBacillus cereus and 17.5 mm against Candida albicans. The crude extractives of all three plants of Bridelia species exhibited cytotoxic activity against brine shrimp nauplii having significant LC50 and LC90.

Research paper thumbnail of Antibacterial and Cytotoxic Constituents from Bridelia verrucosa Haines Growing in Bangladesh

Dhaka University Journal of Pharmaceutical Sciences

Research paper thumbnail of Bioactive Steroid and Triterpenoids from Bridelia stipularis (L) Blume

Dhaka University Journal of Pharmaceutical Sciences, 2017

Research paper thumbnail of <i>In Vitro</i> Antioxidant and Thrombolytic Activities of <i>Bridelia</i> Species Growing in Bangladesh

Journal of Scientific Research, 2013

Research paper thumbnail of In Vitro Antibacterial Activity of Rauvolfia Serpentina and Its Tissue Culture

Nigerian Journal of Natural Products and Medicine, 2002

Abstract The in vitro antibacterial activity of the methanol extracts of leaf, root of Rauvolfia ... more Abstract The in vitro antibacterial activity of the methanol extracts of leaf, root of Rauvolfia serpentina and its tissue culture callus, root, leaf were investigated. The extract showed a good antibacterial activity against gram negative organisms, which may be due to the ...

Research paper thumbnail of Antibacterial compounds from the flowers of Alangium salviifolium

Fitoterapia, 2002

1-Methyl-1H-pyrimidine-2,4-dione and 3-O-beta-D-glucopyranosyl-(24beta)-ethylcholesta-5,22,25-tri... more 1-Methyl-1H-pyrimidine-2,4-dione and 3-O-beta-D-glucopyranosyl-(24beta)-ethylcholesta-5,22,25-triene, isolated from the flowers of Alangium salviifolium, showed remarkable antibacterial activities against a number of Gram-positive and Gram-negative bacterial species.

Research paper thumbnail of In vitro evaluation of antioxidant potentiality and estimation of total phenolic and flavonoid content of the whole stem of Coffea benghalensis B. Heyne ex Schult

This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity and phospho molybdenum assay compared to the reference standard in a dose dependent manner. It was observed that the test samples contained considerable amount of bioactive compounds including total phenolic (ethyl acetate soluble fraction giving the highest 22.631 ± 0.085 GAE/gm of dried sample) and flavonoid (pet ether soluble fraction giving the highest 54.513 ± 1.500 CE/gm of dried sample) content. It can be concluded from the results that the crude methanolic extract along with it's four soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh are a good source of natural antioxidants.

Research paper thumbnail of In vitro evaluation of antioxidant potentiality and estimation of total phenolic and flavonoid content of the whole stem of Coffea benghalensis B. Heyne ex Schult

This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity and phospho molybdenum assay compared to the reference standard in a dose dependent manner. It was observed that the test samples contained considerable amount of bioactive compounds including total phenolic (ethyl acetate soluble fraction giving the highest 22.631 ± 0.085 GAE/gm of dried sample) and flavonoid (pet ether soluble fraction giving the highest 54.513 ± 1.500 CE/gm of dried sample) content. It can be concluded from the results that the crude methanolic extract along with it's four soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh are a good source of natural antioxidants.

Research paper thumbnail of In vitro evaluation of antioxidant potentiality and estimation of total phenolic and flavonoid content of the whole stem of Coffea benghalensis B. Heyne ex Schult

The Pharma Innovation Journal, 2021

Research paper thumbnail of Sub-Acute Toxicity of a Steroidal Glycoside from the Flowers of Alangium Salviifolium Wang

International Research Journal Of Pharmacy

The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl... more The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl-(24)-ethylcholesta-5,22,25-triene, a steroidal glycoside isolated from the flowers of Alangium salviifolium Wang on Long Evan’s rat. After intra-peritoneal administration of the compound at a dose of 300 μg/rat/day for 14 consecutive days, no mortality or significant changes in body weight or behavior were observed. The blood samples of the rats were examined for hematological and biochemical parameters which were statistically insignificant when compared to that of the control group. All the vital organs showed normal histopathological architecture (heart, lungs, liver and kidney) in comparison to the control group. This preliminary investigation demonstrate that the compound is safe at dose of 300 μg/rat/day for 14 consecutive days. But acute, sub-chronic and chronic toxicity evaluations as well as clinical trials need to be done.

Research paper thumbnail of In vitro antioxidant activities of the roots of Coffea benghalensis B Heyne ex Schult. Growing in Bangladesh

Journal of Pharmacognosy and Phytochemistry, 2021

Research paper thumbnail of Evaluation of in vitro antibacterial and in vivo cytotoxic activities of Bangladeshi Coffea benghalensis B Heyne ex Schult. roots

The Journal of Phytopharmacology, 2021

This study was designed to evaluate the in vitro antibacterial and in vivo cytotoxic activity of ... more This study was designed to evaluate the in vitro antibacterial and in vivo cytotoxic activity of the roots of Bangladeshi Coffea benghalensis B Heyne ex Schult. (Family: Rubiaceae), locally known as bonnyo kofee native to the regions of Sylhet and Chittagong in Bangladesh. The finely crushed coarse powder was extracted with methanol and solvent-solvent partitioning was done to obtain n-hexane, chloroform, ethyl acetate and aqueous soluble fractions. The crude methanol extract along with it’s four soluble fractions were then evaluated for their in vitro antibacterial and in vivo cytotoxic activities. The disc diffusion method was used to evaluate the antibacterial activity at a concentration of 200 and 400 μg/disc. All the soluble fractions and the crude methanolic extract did not demonstrate any zone of inhibition. In case of brine shrimp lethality bioassay, all the test samples exhibited cytotoxic activity against brine shrimp nauplii having remarkable LC50 and LC90 values. The n-h...

Research paper thumbnail of Assessment of Antioxidant, Antimicrobial, Cytotoxicity and Thrombolytic Potential of Erythrina fusca Lour Grown in Bangladesh

Bangladesh Pharmaceutical Journal, 2022

The current study was conducted to assess the antioxidant, antimicrobial, cytotoxicity and thromb... more The current study was conducted to assess the antioxidant, antimicrobial, cytotoxicity and thrombolytic potential of the leaves and stem bark of Erythrina fusca Lour grown in Bangladesh. For the assessment of antioxidant properties, DPPH scavenging and phosphomolybdenum assays were used. In the DPPH free radical scavenging assay, the chloroform soluble fraction of a methanol extract of leaves of E. fusca exhibited the highest free radical scavenging activity with an IC50 value of 5.5 ± 0.60 μg/ml, while the reference standard (ascorbic acid) showed an IC50 value of 5.8 ± 0.18 μg/ml. All the test samples and the standard BHT demonstrated good linear relationship in the phosphomolybdenum assay. It was observed that the test samples contained considerable number of bioactive compounds including total phenolics in the aqueous soluble fraction of the leaves giving the highest 94.02 ± 1.004 GAE/100g of dried sample. All test samples exhibited moderate antimicrobial activity against 13 Gra...

Research paper thumbnail of Flavonoid, pterocarpans and steroid from Erythrina fusca Lour. growing in Bangladesh: isolation, and antimicrobial and free-radical scavenging activity

Journal of Medicinal Plants, 2021

Research paper thumbnail of In vitro evaluation of antioxidant potentiality and estimation of total phenolic and flavonoid content of the whole stem of Coffea benghalensis B. Heyne ex Schult

The Pharma Innovation Journal, 2021

This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity ...

Research paper thumbnail of Sub-Acute Toxicity of a Steroidal Glycoside from the Flowers of Alangium Salviifolium Wang

International Research Journal Of Pharmacy, 2021

The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl... more The current study was carried out to investigate the sub-acute toxicity of 3-O--D-glucopyranosyl-(24)-ethylcholesta-5,22,25-triene, a steroidal glycoside isolated from the flowers of Alangium salviifolium Wang on Long Evan’s rat. After intra-peritoneal administration of the compound at a dose of 300 μg/rat/day for 14 consecutive days, no mortality or significant changes in body weight or behavior were observed. The blood samples of the rats were examined for hematological and biochemical parameters which were statistically insignificant when compared to that of the control group. All the vital organs showed normal histopathological architecture (heart, lungs, liver and kidney) in comparison to the control group. This preliminary investigation demonstrate that the compound is safe at dose of 300 μg/rat/day for 14 consecutive days. But acute, sub-chronic and chronic toxicity evaluations as well as clinical trials need to be done.

Research paper thumbnail of Phytochemical and Biological Investigation of Bridelia tomentosa Blume Growing in Bangladesh

Dhaka University Journal of Pharmaceutical Sciences

Bridelia tomentosa Blume is a small evergreen tree which has enormous folklore applications in th... more Bridelia tomentosa Blume is a small evergreen tree which has enormous folklore applications in the treatment of colic, traumatic injury, epidemic influenza and neurasthenia. The main purpose of this study was to isolate bioactive compounds from the stem bark of B. tomentosa growing in Bangladesh. Extensive chromatographic separation and purification of the methanolic extract of stem bark of B. tomentosa led to the isolation of four compounds. The purified constituents were identified as friedoolean-5(6),14(15)-dien-3-one (1), β-taraxerol (2), D4- stigmasterone (3) and lupeol (4) by extensive analysis of NMR spectroscopic data. While compound 1 is appeared to be new, the other compounds (2-4) have been isolated for the first time from this plant. β-taraxerol (2) demonstrated significant cytotoxic activity against the brine shrimp Artemia salina and moderate to strong antimicrobial efficacy with the highest inhibitory potential against Salmonella Typhi (zone of inhibition = 21.3 mm) a...

Research paper thumbnail of In vitro evaluation of antioxidant potentiality and estimation of total phenolic and flavonoid content of the whole stem of Coffea benghalensis B. Heyne ex Schult

The Pharma Innovation Journal, 2021

This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity ...

Research paper thumbnail of In vitro antibacterial, antifungal and cytotoxic activity of three Bangladeshi Bridelia species

International research journal of pharmacy, 2011

The organic soluble extractives of three Bridelia species, B. verrucosa, B. stipularis and B. tom... more The organic soluble extractives of three Bridelia species, B. verrucosa, B. stipularis and B. tomentosa growing in Bangladesh were subjected to screening for antibacterial, antifungal and cytotoxic activities. All extractives showed moderate to strong antimicrobial activity against 13 Gram positive and Gram negative bacterial strains and three fungi where the stem bark of B. tomentosa demonstrated highest inhibition of growth with zone of inhibition of 23.2 mm againstBacillus cereus and 17.5 mm against Candida albicans. The crude extractives of all three plants of Bridelia species exhibited cytotoxic activity against brine shrimp nauplii having significant LC50 and LC90.

Research paper thumbnail of Antibacterial and Cytotoxic Constituents from Bridelia verrucosa Haines Growing in Bangladesh

Dhaka University Journal of Pharmaceutical Sciences

Research paper thumbnail of Bioactive Steroid and Triterpenoids from Bridelia stipularis (L) Blume

Dhaka University Journal of Pharmaceutical Sciences, 2017

Research paper thumbnail of <i>In Vitro</i> Antioxidant and Thrombolytic Activities of <i>Bridelia</i> Species Growing in Bangladesh

Journal of Scientific Research, 2013

Research paper thumbnail of In Vitro Antibacterial Activity of Rauvolfia Serpentina and Its Tissue Culture

Nigerian Journal of Natural Products and Medicine, 2002

Abstract The in vitro antibacterial activity of the methanol extracts of leaf, root of Rauvolfia ... more Abstract The in vitro antibacterial activity of the methanol extracts of leaf, root of Rauvolfia serpentina and its tissue culture callus, root, leaf were investigated. The extract showed a good antibacterial activity against gram negative organisms, which may be due to the ...

Research paper thumbnail of Antibacterial compounds from the flowers of Alangium salviifolium

Fitoterapia, 2002

1-Methyl-1H-pyrimidine-2,4-dione and 3-O-beta-D-glucopyranosyl-(24beta)-ethylcholesta-5,22,25-tri... more 1-Methyl-1H-pyrimidine-2,4-dione and 3-O-beta-D-glucopyranosyl-(24beta)-ethylcholesta-5,22,25-triene, isolated from the flowers of Alangium salviifolium, showed remarkable antibacterial activities against a number of Gram-positive and Gram-negative bacterial species.

Research paper thumbnail of In vitro evaluation of antioxidant potentiality and estimation of total phenolic and flavonoid content of the whole stem of Coffea benghalensis B. Heyne ex Schult

This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity and phospho molybdenum assay compared to the reference standard in a dose dependent manner. It was observed that the test samples contained considerable amount of bioactive compounds including total phenolic (ethyl acetate soluble fraction giving the highest 22.631 ± 0.085 GAE/gm of dried sample) and flavonoid (pet ether soluble fraction giving the highest 54.513 ± 1.500 CE/gm of dried sample) content. It can be concluded from the results that the crude methanolic extract along with it's four soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh are a good source of natural antioxidants.

Research paper thumbnail of In vitro evaluation of antioxidant potentiality and estimation of total phenolic and flavonoid content of the whole stem of Coffea benghalensis B. Heyne ex Schult

This study was carried out for the first time to evaluate the antioxidant properties and total ph... more This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity and phospho molybdenum assay compared to the reference standard in a dose dependent manner. It was observed that the test samples contained considerable amount of bioactive compounds including total phenolic (ethyl acetate soluble fraction giving the highest 22.631 ± 0.085 GAE/gm of dried sample) and flavonoid (pet ether soluble fraction giving the highest 54.513 ± 1.500 CE/gm of dried sample) content. It can be concluded from the results that the crude methanolic extract along with it's four soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh are a good source of natural antioxidants.