Jan Kusznierz | University of Wroclaw (original) (raw)
Papers by Jan Kusznierz
Annales Zoologici Fennici, 2004
Remains of Coregonus sp. were obtained from the collection of archeological materials found in 19... more Remains of Coregonus sp. were obtained from the collection of archeological materials found in 1991 at the site Wola Grzymalina, Poland. DNA has been extracted from vertebrae that were dated about 500 000 years old. Polymerase chain reactions on extracts from the vertebral remains were succesful only using primers that targeted nucleotide sequences that were not longer than about 100bp. The ancient nucleotide sequence of a 102bp fragment of mitochondrial DNA ND1 gene matched the contemporary sequence of European whitefi sh (Coregonus lavaretus) and differed from that of vendace (C. albula) by four substitutions. Ambiguous nucleotides were observed at nine positions throughout the 87bp fragment of mtDNA control region (CR), nonetheless the sequence was different from that of vendace by eight additional changes. Phylogenetic analysis utilizing Bayesian inference placed the archival sequence into a clade with European whitefi sh. The support for this reconstruction was f (t|X) = 0.65 after analysis of the ND1 fragment and f (t|X) = 0.52 after analysis of the CR fragment. Excellent preservation of the specimen analysed in this study proves the potential for analysing genetic characteristics of species and specimens that are thousands of years old.
The European zoological journal, Jan 30, 2023
Przegląd Zoologiczny, 1995
Teka Komisji Ochrony i Kształtowania Środowiska Przyrodniczego, 2008
Summary. The present study was the first attempt at evaluat ing the size and sex structure of the... more Summary. The present study was the first attempt at evaluat ing the size and sex structure of the population of lake minnow Eupallasella percnurus , a critically endangered in Poland cyprinid fish species, by means of the capture-recapture Lincoln- Petersen method. The study area was a small (approx. 0.1 ha) man-made fire reservoir located ne ar town of Zielonka in the Mazowieckie Voivodeship. To assess the age structure of the pop ulation, frequency distribution of fish total length was used. The total size of the population w as estimated at 2000‐2500 individuals, including males, females and juveniles aged 1+ or older. Male-to-female ratio in mature individuals was found to be 1 : 2. Young fish belonging to 1+ and 2 + age classes dominated in the population, which may indicate that it is overcrowded. The capt ure-recapture method appears to be useful in the spawning period for the evaluation of the size and structure of E. percnurus population, provided that fish behaviour ‐ considerably influen cing fish recapture rates ‐ is taken into considera tion.
Parki Narodowe i Rezerwaty Przyrody, 2004
Komunikaty Rybackie, 2002
Archives of Polish Fisheries, 2004
The growth, condition and survival of live food-fed larvae and starter-fed early juveniles of the... more The growth, condition and survival of live food-fed larvae and starter-fed early juveniles of the critically endangered cyprinid Eupallasella perenurus (Pallas) were evaluated in two laboratory experiments at 19, 22, 25, and 28°C. Temperature significantly (P £ 0.05) influenced the growth of larvae, with the maximum final values recorded at 25°C (25.8 mm mean total length, TL; 239 mg mean body weight, BW). No significant differences in the final juvenile fish size were found within the temperature range of 19-25°C (37.6-38.4 mm TL; 901-968 mg BW), whereas those at 28°C grew slower (34.6 mm TL; 819 mg BW). The final condition coefficient values tended to significantly increase along with water temperature. Final survival rates were 96.5-100%, except for juveniles at 28°C (28.3%). All of the present results indicate that the most advantageous temperature conditions for E. perenurus controlled rearing would be 25 and 22°C for larvae and juveniles, respectively.
Aquaculture International, 2004
The standard artificial reproduction technique with the use of Ovopel (GnRH analogue) was evaluat... more The standard artificial reproduction technique with the use of Ovopel (GnRH analogue) was evaluated for the critically endangered cyprinid species in Poland, the lake minnow Eupallasella perenurus. Wild spawners were angled at the beginning of the spawning season. Only females with a condition coefficient value higher than the mean were included in the experiment and then stimulated with Ovopel. Group A (n = 20) was given standard treatment (0.2 pellet kg −1 and 1.0 pellet kg −1 after 12 h), whereas group B (n = 8) was treated with a single dose of 2.0 pellets kg −1. After fertilisation with the dry method, the glutinous eggs attached to the bottom of flow-through aquaria were incubated at 17.1-18.4 • C. Throughout incubation (85D •), dead eggs were removed and counted. The same was done with dead larvae, those with deformed bodies or those with empty alimentary tracts after 2 days of external feeding. Two injections of Ovopel resulted in a significantly (P ≤ 0.05) higher ovulation rate in comparison with the single dose (70 and 25% in groups A and B, respectively). The individual hatching rates were very high (98.4-100%), as was the share of good quality larvae (91.1 and 96.5% of stripped eggs in groups A and B, respectively). These results indicate that the standard propagation method used with commercially important cyprinid species can also be used to successfully breed E. perenurus.
Archives of Polish Fisheries, 2011
The lake minnow, Eupallasella percnurus (Pall.), has not been subject to any comprehensive ichthy... more The lake minnow, Eupallasella percnurus (Pall.), has not been subject to any comprehensive ichthyoparasitological study; most information on its parasites comes from studies focused on other fish species. The knowledge of the geographical distribution of its parasites is especially incomplete. This paper presents the most up-to-date list of E. percnurus parasites throughout its distribution range. Most of the trematodes, tapeworms, and nematodes found in this host are larval forms. This means that the parasites use the E. percnurus mainly as an intermediate or paratenic host, and not the definitive host, which is important in light of potential parasite pathogenicity.
Annales Zoologici Fennici, 2014
The Eurasian minnow Phoxinus phoxinus is widely distributed across most of Europe and northern As... more The Eurasian minnow Phoxinus phoxinus is widely distributed across most of Europe and northern Asia. It displays considerable morphological diversity and variable colouration. Despite this it is still believed that its whole distribution holds only one monotypic species. There are no previous conclusive morphometric analyses of samples from different parts of its distribution area. This paper presents results of multidimensional analyses of morphometric characters of 14 widely scattered populations of the species. Our analyses show considerable inter-population differences. Morphometric evidence suggests that populations from the Amur River system and central Asia may represent new, distinct species or subspecies. The status of the remaining populations remains unclear.
Caryologia, 1997
The karyotype of swamp minnow, Eupallasel/.a percnurus (= Phoxinus percnurus) from northern part ... more The karyotype of swamp minnow, Eupallasel/.a percnurus (= Phoxinus percnurus) from northern part of Poland was studied by conventional Giemsa staining and Ag-NOR technique. The diploid chromosome number 2n =50, was composed of 5 pairs of metacentric, 15 pairs of submetacentric and 5 pairs of subtelo-to acrocentric chromosomes (NF = 90). Two pairs of NORs bearing chromosome were detected. They were located terminally on the short arms of one pair of medium sized submetacentric and on one pair of medium sized subtelocentric chromosomes. Most of the related species from the genus Phoxinus and Moroco had diploid chromosome number 2n =50 and their karyotypes were also characterized by the biggest acrocentric pair. Most of the North American minnows species had one pair of NORs bearing chromosomes.
Archives of Polish Fisheries, 2013
Representatives of the genera Hucho and Parahucho are examples of fish that have yet been subject... more Representatives of the genera Hucho and Parahucho are examples of fish that have yet been subjects of comprehensive parasitological examination. This paper presents the most current list of parasites of the three best documented species of the genera Hucho (Hucho hucho, Hucho taimen) and Parahucho (Parahucho perryi) from the entire range of their occurrence. Notably, the decided majority of the parasitic worms, or helminths, identified in these hosts (trematodes, cestodes, nematodes) are represented by intestinal adult forms. This indicates that the fish of the genera Hucho and Parahucho are the definitive hosts of these parasites, as opposed to that of intermediate or paratenic host. This fact is significant in terms of potential parasitic pathogenesis and the consequent lowered host resistance to disadvantageous environmental impacts.
Fisheries Management and Ecology, 2006
The lake minnow, Eupallasella percnurus (Pallas), is a small cyprinid fish with a broad Palaearct... more The lake minnow, Eupallasella percnurus (Pallas), is a small cyprinid fish with a broad Palaearctic distribution. In Europe it inhabits almost exclusively small, strictly isolated water bodies, especially dystrophic or shallow eutrophic lakes, as well as pools left after peat cutting (Kusznierz 2001). In the Siberian part of its
Annales Zoologici Fennici, 2004
Remains of Coregonus sp. were obtained from the collection of archeological materials found in 19... more Remains of Coregonus sp. were obtained from the collection of archeological materials found in 1991 at the site Wola Grzymalina, Poland. DNA has been extracted from vertebrae that were dated about 500 000 years old. Polymerase chain reactions on extracts from the vertebral remains were succesful only using primers that targeted nucleotide sequences that were not longer than about 100bp. The ancient nucleotide sequence of a 102bp fragment of mitochondrial DNA ND1 gene matched the contemporary sequence of European whitefi sh (Coregonus lavaretus) and differed from that of vendace (C. albula) by four substitutions. Ambiguous nucleotides were observed at nine positions throughout the 87bp fragment of mtDNA control region (CR), nonetheless the sequence was different from that of vendace by eight additional changes. Phylogenetic analysis utilizing Bayesian inference placed the archival sequence into a clade with European whitefi sh. The support for this reconstruction was f (t|X) = 0.65 after analysis of the ND1 fragment and f (t|X) = 0.52 after analysis of the CR fragment. Excellent preservation of the specimen analysed in this study proves the potential for analysing genetic characteristics of species and specimens that are thousands of years old.
The European zoological journal, Jan 30, 2023
Przegląd Zoologiczny, 1995
Teka Komisji Ochrony i Kształtowania Środowiska Przyrodniczego, 2008
Summary. The present study was the first attempt at evaluat ing the size and sex structure of the... more Summary. The present study was the first attempt at evaluat ing the size and sex structure of the population of lake minnow Eupallasella percnurus , a critically endangered in Poland cyprinid fish species, by means of the capture-recapture Lincoln- Petersen method. The study area was a small (approx. 0.1 ha) man-made fire reservoir located ne ar town of Zielonka in the Mazowieckie Voivodeship. To assess the age structure of the pop ulation, frequency distribution of fish total length was used. The total size of the population w as estimated at 2000‐2500 individuals, including males, females and juveniles aged 1+ or older. Male-to-female ratio in mature individuals was found to be 1 : 2. Young fish belonging to 1+ and 2 + age classes dominated in the population, which may indicate that it is overcrowded. The capt ure-recapture method appears to be useful in the spawning period for the evaluation of the size and structure of E. percnurus population, provided that fish behaviour ‐ considerably influen cing fish recapture rates ‐ is taken into considera tion.
Parki Narodowe i Rezerwaty Przyrody, 2004
Komunikaty Rybackie, 2002
Archives of Polish Fisheries, 2004
The growth, condition and survival of live food-fed larvae and starter-fed early juveniles of the... more The growth, condition and survival of live food-fed larvae and starter-fed early juveniles of the critically endangered cyprinid Eupallasella perenurus (Pallas) were evaluated in two laboratory experiments at 19, 22, 25, and 28°C. Temperature significantly (P £ 0.05) influenced the growth of larvae, with the maximum final values recorded at 25°C (25.8 mm mean total length, TL; 239 mg mean body weight, BW). No significant differences in the final juvenile fish size were found within the temperature range of 19-25°C (37.6-38.4 mm TL; 901-968 mg BW), whereas those at 28°C grew slower (34.6 mm TL; 819 mg BW). The final condition coefficient values tended to significantly increase along with water temperature. Final survival rates were 96.5-100%, except for juveniles at 28°C (28.3%). All of the present results indicate that the most advantageous temperature conditions for E. perenurus controlled rearing would be 25 and 22°C for larvae and juveniles, respectively.
Aquaculture International, 2004
The standard artificial reproduction technique with the use of Ovopel (GnRH analogue) was evaluat... more The standard artificial reproduction technique with the use of Ovopel (GnRH analogue) was evaluated for the critically endangered cyprinid species in Poland, the lake minnow Eupallasella perenurus. Wild spawners were angled at the beginning of the spawning season. Only females with a condition coefficient value higher than the mean were included in the experiment and then stimulated with Ovopel. Group A (n = 20) was given standard treatment (0.2 pellet kg −1 and 1.0 pellet kg −1 after 12 h), whereas group B (n = 8) was treated with a single dose of 2.0 pellets kg −1. After fertilisation with the dry method, the glutinous eggs attached to the bottom of flow-through aquaria were incubated at 17.1-18.4 • C. Throughout incubation (85D •), dead eggs were removed and counted. The same was done with dead larvae, those with deformed bodies or those with empty alimentary tracts after 2 days of external feeding. Two injections of Ovopel resulted in a significantly (P ≤ 0.05) higher ovulation rate in comparison with the single dose (70 and 25% in groups A and B, respectively). The individual hatching rates were very high (98.4-100%), as was the share of good quality larvae (91.1 and 96.5% of stripped eggs in groups A and B, respectively). These results indicate that the standard propagation method used with commercially important cyprinid species can also be used to successfully breed E. perenurus.
Archives of Polish Fisheries, 2011
The lake minnow, Eupallasella percnurus (Pall.), has not been subject to any comprehensive ichthy... more The lake minnow, Eupallasella percnurus (Pall.), has not been subject to any comprehensive ichthyoparasitological study; most information on its parasites comes from studies focused on other fish species. The knowledge of the geographical distribution of its parasites is especially incomplete. This paper presents the most up-to-date list of E. percnurus parasites throughout its distribution range. Most of the trematodes, tapeworms, and nematodes found in this host are larval forms. This means that the parasites use the E. percnurus mainly as an intermediate or paratenic host, and not the definitive host, which is important in light of potential parasite pathogenicity.
Annales Zoologici Fennici, 2014
The Eurasian minnow Phoxinus phoxinus is widely distributed across most of Europe and northern As... more The Eurasian minnow Phoxinus phoxinus is widely distributed across most of Europe and northern Asia. It displays considerable morphological diversity and variable colouration. Despite this it is still believed that its whole distribution holds only one monotypic species. There are no previous conclusive morphometric analyses of samples from different parts of its distribution area. This paper presents results of multidimensional analyses of morphometric characters of 14 widely scattered populations of the species. Our analyses show considerable inter-population differences. Morphometric evidence suggests that populations from the Amur River system and central Asia may represent new, distinct species or subspecies. The status of the remaining populations remains unclear.
Caryologia, 1997
The karyotype of swamp minnow, Eupallasel/.a percnurus (= Phoxinus percnurus) from northern part ... more The karyotype of swamp minnow, Eupallasel/.a percnurus (= Phoxinus percnurus) from northern part of Poland was studied by conventional Giemsa staining and Ag-NOR technique. The diploid chromosome number 2n =50, was composed of 5 pairs of metacentric, 15 pairs of submetacentric and 5 pairs of subtelo-to acrocentric chromosomes (NF = 90). Two pairs of NORs bearing chromosome were detected. They were located terminally on the short arms of one pair of medium sized submetacentric and on one pair of medium sized subtelocentric chromosomes. Most of the related species from the genus Phoxinus and Moroco had diploid chromosome number 2n =50 and their karyotypes were also characterized by the biggest acrocentric pair. Most of the North American minnows species had one pair of NORs bearing chromosomes.
Archives of Polish Fisheries, 2013
Representatives of the genera Hucho and Parahucho are examples of fish that have yet been subject... more Representatives of the genera Hucho and Parahucho are examples of fish that have yet been subjects of comprehensive parasitological examination. This paper presents the most current list of parasites of the three best documented species of the genera Hucho (Hucho hucho, Hucho taimen) and Parahucho (Parahucho perryi) from the entire range of their occurrence. Notably, the decided majority of the parasitic worms, or helminths, identified in these hosts (trematodes, cestodes, nematodes) are represented by intestinal adult forms. This indicates that the fish of the genera Hucho and Parahucho are the definitive hosts of these parasites, as opposed to that of intermediate or paratenic host. This fact is significant in terms of potential parasitic pathogenesis and the consequent lowered host resistance to disadvantageous environmental impacts.
Fisheries Management and Ecology, 2006
The lake minnow, Eupallasella percnurus (Pallas), is a small cyprinid fish with a broad Palaearct... more The lake minnow, Eupallasella percnurus (Pallas), is a small cyprinid fish with a broad Palaearctic distribution. In Europe it inhabits almost exclusively small, strictly isolated water bodies, especially dystrophic or shallow eutrophic lakes, as well as pools left after peat cutting (Kusznierz 2001). In the Siberian part of its