Effects of Different Drying Methods and Storage Time on Free Radical Scavenging Activity and Total Phenolic Content of Cosmos Caudatus (original) (raw)
Related papers
2014
The present study was conducted to determine the effect of air (AD), oven (OD) and freeze drying (FD) on the free radical scavenging activity and total phenolic content (TPC) of Cosmos caudatus and the effect of storage time by the comparison with a fresh sample (FS). Among the three drying methods that were used, AD resulted in the highest free radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC 50 = 0.0223 mg/mL) and total phenolic content (27.4 g GAE/100 g), whereas OD produced the lowest scavenging activity and TPC value. After three months of storage, the dried samples showed a high and consistent free radical scavenging activity when compared to stored fresh material. The drying methods could preserve the quality of C. caudatus during storage and the stability of its bioactive components can be maintained.
Molecules, 2013
The aim of the study was to analyze the influence of oven thermal processing of Cosmos caudatus on the total polyphenolic content (TPC) and antioxidant capacity (DPPH) of two different solvent extracts (80% methanol, and 80% ethanol). Sonication was used to extract bioactive compounds from this herb. The results showed that the optimised conditions for the oven drying method for 80% methanol and 80% ethanol were 44.5 °C for 4 h with an IC 50 of 0.045 mg/mL and 43.12 °C for 4.05 h with an IC 50 of 0.055 mg/mL, respectively. The predicted values for TPC under the optimised conditions for 80% methanol and 80% ethanol were 16.5 and15.8 mg GAE/100 g DW, respectively. The results obtained from this study demonstrate that Cosmos caudatus can be used as a potential source of antioxidants for food and medicinal applications.
Journal of Food and Dairy Sciences
The effect of antioxidant properties as free radical scavenging activity and phytochemical compounds of fresh and dried samples of dill, parsley, coriander, peppermint and celery leaves were studied. Moreover, the chemical constituents and the content of minerals in these medicinal and aromatic plants were also investigated. Results explained that the contents of total phenolics ranged from 1446.88 for coriander to 3128.57mg/100gm for peppermint and after the drying process decreased to 1280.10 and 2356.45mg/100gm, respectively. Also, peppermint had the highest content of flavonoids, while the lowest content was observed in coriander leaves. Otherwise, fresh coriander leaves had the highest content of carotenoids, while the lowest content was in the fresh peppermint leaves. Results explained also that the main component of phenolic compounds was protocatchuic in all fresh and dried samples under investigation. Celery leaves had the highest protocatchuic content. Concerning minerals content reveal that the parsley leaves had the highest content of calcium, potassium and magnesium, while peppermint had the highest phosphorus and iron contents. Results also show that celery leaves had the highest sodium content. Fresh dill and peppermint recorded the highest percentages of total antioxidant activity. Also, fresh celery had gradual effect on DPPH compounds being used as synthetic free radical. Coriander and parsley showed the lowest antioxidant activity. After the drying process total antioxidant activity decreased. Slight decrease was observed with dill and peppermint leaves, while celery and parsley leaves were more effective by using the air oven drier process.
2018
Herbs and medicinal plants are major sources of traditional or folk medicines for many countries of the world, including Malaysia. This study evaluated the bioactive potential of the leaf ethanolic extract and solvent fractions of Cosmos caudatus Kunth, in scavenging free radicals and inhibiting the enzyme α-glucosidase. In addition, their metabolite profiles were also characterized using liquid chromatography–mass spectrometry. The bioactivity was found to be concentrated in the EtOAc and BuOH fractions which largely contained rutin, quercetin 3-O-galactoside, quercetin 3-O-glucoside, quercetin 3-O-xyloside, quercetin 3-O-arabinofuranoside, quercetin 3-O-rhamnoside, and quercetin 3-O-galactoside, as profiled by LC-MS/MS. It was further shown that the flavonoids glycosides contributed to the free radical scavenging and glucose lowering effects of C. caudatus leaves. The results indicated that the leaves of C. caudatus are a rich source of bioactive compounds and could be prospective...
Therefore, this study investigated antioxidant activity of Cosmos caudatus leaves at three different maturity stages (young, mature and old leaves) extracted using two extraction solvents (water and 95% ethanol). The analyses of antioxidant activity conducted were total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays. From the analyses, young leaves showed significantly higher antioxidant activity compared to mature and old leaves. However, C. caudatus leaves extracted using water had significantly higher in TPC, TFC and FRAP values than those of ethanolic extracts, whereas opposite trend was observed in DPPH radical scavenging assay. Based on Pearson's correlation coefficient, TPC and TFC for all extract exhibited strong correlations with antioxidant assays indicating that these leaves have potent phenolic and flavonoid compounds. In fact, most phenolic and flavonoid compounds including gallic acid, chlorogenic acid, caffeic acid, vanillic acid, ρ-coumaric acid, sinapic acid, ferullic acid, catechin, epicatechin, rutin, myricetin and quercetin, except naringenin and kaempferol were detected by High Performance Liquid Chromatography (HPLC) in all samples for both extracts. Hence, it can be concluded that C. caudatus young leaves extracted using water had the most powerful antioxidant, suggesting their potential application as health-promoting functional ingredients or natural preservatives in food and pharmaceutical products.
Food Research, 2018
Two different drying methods-freeze drying and vacuum oven drying were applied to Ocimum basilicum leaves to determine the proximate composition and antioxidant activities. The results were compared to fresh leaves of Ocimum basilicum. Each sample was prepared for determination in three different solvents-methanol, ethanol, and water have been used. The total phenolic content (TPC) and total flavonoid content (TFC) assays showed almost the same values in the freeze-dried sample of methanol extract as it exhibited the highest TPC and TFC values-54.46±1.24 mg gallic acid/g, 950±17.32 mg quercetin/g whereas the water extract showed only 26.23±0.63 mg gallic acid/g and 543.33±56.86 mg quercetin/g, respectively. On the contrary, fresh sample with water extract displayed the lowest TPC and TFC. In addition, the percent inhibition of 1,1diphenyl-2-2picrylhydrazyl (DPPH) free radical-scavenging assay ranged from 6.09± 0.47% in fresh sample with water extract to 92.60±0.58% in freeze-dried sample with methanol extract. The ferric reducing antioxidant power (FRAP) assay values ranged from 7.62±1.09 µmol FeII/g in fresh samples with water extract to 1,160.95±15.80 µmol FeII/g in freeze-dried sample with methanol extract. Both assays showed the same trend of result with the highest antioxidant activity observed in freeze-dried sample with methanol extract and the lowest from fresh samples with water extract.
Molecules, 2019
Rhus pachyrrhachis and Rhus virens are medicinal plant species with important uses in northeastern Mexico. They belong to a complex of Rhus species called “lantriscos”, which are used for medicinal applications. The medicinal effects of these species are based on traditional use, however, they require phytochemical research to validate their medicinal properties, as well as structural characterization for their correct identification during the collecting practice and uses. The phytochemical potential of aqueous extracts from R. pachyrrhachis and R. virens was analyzed by the quantification of total phenolic content (TPC), free radical-scavenging potential, and total flavonoids, with a comparison of four drying methods, and some phenolic compounds were identified. Furthermore, the stems and leaves of both species were anatomically characterized to establish a differentiation. R. pachyrrhachis and R. virens showed similar values of phytochemical contents, although the TPC content (0....
Radical scavenging activity of plant extracts from improved processing
Heliyon, 2019
Radical scavenging activity of extracts obtained from 16 plants harvested in South Hungary was assessed and compared to the activity of ascorbic acid standard. During extraction, a novel technique involving an ethanolic treatment at ambient temperature was used for advanced active component release. Although the procedure is time consuming, it serves as an efficient and harmless route to extract valuable antioxidant compounds from their natural sources. The as-prepared extracts consist of two phases (except Allium sativum), a clear solution and a thick suspension containing solid plant parts that separates in about 2 h. The samples were analysed by the antioxidant assay based on the scavenging of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals. For most of the species, the solid phase retained considerable amount of available antioxidant agents, while the solution parts showed significant radical scavenging activity. The main exceptions were Nigella sativa, Hippophae rhamnoides and Linum usitatissimum, where the solid parts were less active. Overall, the extracts possessed remarkable antioxidant activity that were compared to published literature data and were found to be superior.
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Journal of the University College of Science and Technology (JUCST), 2022
Antioxidant is a substrate that stops molecules inside a cell from oxidizing. During the biological oxidation reaction, free radicals created; Also, it have the ability to stop a chain reaction by removing the free radical intermediate. The aim of the study was conducted to estimate the impact of two drying methods on phytochemical constituents of the Prickly pear fruit (Opuntia ficus indica, L.). Methods: In this work, the effects of oven-drying and freeze-drying methods have been studied in Special Units Departments-National Research Center (Cairo) in august 2021 on the chemical composition, total
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Food Chemistry, 2009
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