Antimicrobial activity of chitosan coated silk fibroin micro-fibers (original) (raw)
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Antibacterial properties of silk fibroin/chitosan blend films loaded with plant extract
Fibers and Polymers, 2010
The silk fibroin (SF)/chitosan (CHI) blend films were prepared by dissolving them in formic acid. The morphology of the films was examined by scanning electron microscopy (SEM). The roughness of the membranes was determined by atomic force microscopy (AFM). These films were treated with the extracts of Pistacia terebinthus, Pistacia lentiscus, and Hypericum empetrifolium. Folin-Ciocalteu assay was used to determine the amount of total phenols adsorbed on these blend films. The antibacterial properties of films were tested using disc diffusion and turbidity measurement methods against Escherichia coli and Staphylococcus epidermidis. The release of adsorbed phenolics from the film surface resulted in antibacterial properties.
Biopolymers are one of the suitable alternate materials for different chemical processes because of its environmental friendly behavior. This research work shows a comparative analysis of silk dye with acid dye before and after Chitosan treatment. In this analysis silk yarns are treated with two different Chitosan 0.5% & 1.0% at temperature (40 & 60)° C respectively and dyed with acid dye at pH 6.00. The study focus on the dye uptake %, color strength value (K/S), different types of fastness properties, antimicrobial properties and the strength of dyed sample. It was found that sample treated with 1% Chitosan showed better dye uptake at 40°C and 60°C are 86.82% and 88.28% respectively, color strength value at 40°C and 60°C are 6.64 and 7.10 respectively and color fastness also increases with increase of temperature. The effects of Chitosan on the antibacterial properties of silk yarns against both of gram positive and gram negative bacteria such as Bacillus cereus, Stophyloccus aureus, Escherichia coli, Salmonella typhi, Psedomonous aeroginosa and Shigella dysenteriae were investigated and show the antibacterial potential due to the antimicrobial property of Chitosan. KEYWORD: Silk fiber, acid dye, chitosan, antimicrobial activity, Scanning electron microscopy and silk dyeing.
Biomedical Materials & Devices
Antibacterial wound dressings have great importance to reduce wound infections caused by bacteria. In this research work, levofloxacin-loaded silk fibroin and chitosan composite scaffolds were synthesized for effective wound dressing applications. Initially, silk was degummed for extraction of silk fibroin, and it was mixed with chitosan at different ratios. The chitosan and silk fibroin were stirred at room temperature to get soluble and clear gel formation. Afterward, citric acid was poured into the solution and stirred for more 30 min to get a homogeneous solution. The chitosan and silk fibroin composite was dried using a vacuum oven at 40 °C for 48 h. Next, the composite was loaded with levofloxacin HCL for 24 h at room temperature. The prepared composite was characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and fourier transform infrared (FTIR) spectroscopy. The antibacterial activity of prepared scaffolds was evaluated by the agar disc diffusion method. The results of FTIR spectroscopy proved that citric acid cross-linked the cross-linked silk fibroin with chitosan. SEM and EDX spectroscopy demonstrated successful loading of levofloxacin onto scaffolds. Moreover, the levofloxacin-loaded scaffold samples confirmed sustained drug release and antibacterial properties. All results demonstrated that the composite scaffolds using silk fibroin and chitosan with an appropriate antibacterial agent could effectively inhibit the growth of bacteria.
Marine Drugs
Marine-derived chitosan (CS) is a cationic polysaccharide widely studied for its bioactivity, which is mostly attached to its primary amine groups. CS is able to neutralize reactive oxygen species (ROS) from the microenvironments in which it is integrated, consequently reducing cell-induced oxidative stress. It also acts as a bacterial peripheral layer hindering nutrient intake and interacting with negatively charged outer cellular components, which lead to an increase in the cell permeability or to its lysis. Its biocompatibility, biodegradability, ease of processability (particularly in mild conditions), and chemical versatility has fueled CS study as a valuable matrix component of bioactive small-scaled organic drug-delivery systems, with current research also showcasing CS’s potential within tridimensional sponges, hydrogels and sutures, blended films, nanofiber sheets and fabric coatings. On the other hand, renewable plant-derived extracts are here emphasized, given their poten...
Chitosan Fibres Modified by Fibroin
Fibres & Textiles in Eastern Europe, 2007
This article presents research results concerning the formation of chitosan fibres modified by silk-fibroin. Silk-fibroin isolated from the Bombyx mori silkworm cocoons and chitosan were used for our investigations. In order to find advantageous fibre-formation conditions, the influence of bath composition and spinning solution composition on the silk-fibroin content in the bath were tested. The best coagulation bath for spinning solution with 5% silk-fibroin content was the aqueous solution of NaOH at concentration of 10%. In the case of spinning solution with 10 and 15% of silk-fibroin the best coagulation bath was aqueous solution of NaOH at concentration of 10% with an ethyl alcohol in the ratio of 70:30. The chitosan/silk-fibroin fibres obtained under these conditions were characterised by a silkfibroin content from 4,5 to 11% and by mechanical properties sufficient for their further processing into dressing materials.
Pharmaceutics
Microporous chitosan nanofibers functionalized with different amounts of an antimicrobial agent via imine linkage were prepared by a three-step procedure including the electrospinning of a chitosan/PEO blend, PEO removal and acid condensation reaction in a heterogeneous system with 2-formylphenylboronic acid. The fibers’ characterization was undertaken keeping in mind their application to wound healing. Thus, by FTIR and 1H-NMR spectroscopy, it was confirmed the successful imination of the fibers and the conversion degree of the amine groups of chitosan into imine units. The fiber morphology in terms of fiber diameter, crystallinity, inter- and intra-fiber porosity and strength of intermolecular forces was investigated using scanning electron microscopy, polarized light microscopy, water vapor sorption and thermogravimetric analysis. The swelling ability was estimated in water and phosphate buffer by calculating the mass equilibrium swelling. The fiber biodegradation was explored in...
World journal of microbiology & biotechnology, 2018
Over the years the body of work relating chitosan and its possible skin-related applications has grown, showing that chitosan is active both as a free compound and as a functional finishing of textiles. As a free molecule chitosan proved itself to be an attractive option as it is biocompatible and has a demonstrated biological activity (e.g. growth inhibition or adhesion inhibition) upon multiple skin pathogens, even upon multidrug resistant species. Furthermore, chitosan has wound healing accelerating properties, which make it a prime candidate for possible control of skin related infections. Almost inconspicuously, textiles have been one the main lines of defense of the skin against foreign threats, acting as a physical barrier to microbial colonization and infection. With the advent of textile functionalization specially designed textiles with enhanced protective characteristics, such as antimicrobial capacity, have come to the forefront. Chitosan functionalized textiles have bee...
Chitosan: antimicrobial action upon staphylococci after impregnation onto cotton fabric
Journal of Applied Microbiology, 2012
Background: High levels of viable Staphylococcus aureus, which are often found on inflamed skin surfaces, are usually associated with atopic dermatitis. Textiles, owing to their high specific surface area and intrinsic hydrophilicity, retain moisture while also providing excellent environmental conditions for microbial growth and proliferation. Recently, a number of chemicals have been added to textiles, so as to confer antimicrobial activity. Aims: To evaluate the antimicrobial action of chitosan upon selected skin staphylococci. Methods and Results: We isolated staphylococci from normal skin of 24 volunteers and studied their survival upon contact with chitosan-impregnated cotton fabric. Low and high molecular weight chitosans were used at two concentrations; all four did effectively reduce the growth of some staphylococci (namely Staph. aureus), by up to 5 log cycles, thus unfolding a potential towards control and even prevention of related skin disorders. Conclusion: Our data suggest an effective, but selective antibacterial action of chitosans towards skin bacteria. Significance and Impact of the Study: The possibility to use a natural biopolymer incorporated in a textile to alleviate and even treat some of the symptoms associated with this skin condition may raise an alternative to existing medical treatments. The selectivity observed prevents full elimination of bacteria from the skin surface, which is an advantage.
Materials, 2021
This research proposed two pretreatments of viscose fabrics: oxidation with 2,2,6,6-tetramethylpiperidine-1-oxy radical (TEMPO) and coating with TEMPO-oxidized cellulose nanofibrils (TOCN), to introduce functional groups (COOH and CHO) suitable for irreversible binding of chitosan nanoparticles without and with embedded zinc (NCS and NCS + Zn, respectively) and consequently achieving washing durable antibacterial properties of the chitosan nanoparticles functionalized fabrics. The characterizations of pretreated and chitosan nanoparticles functionalized fabrics were performed by FTIR and XPS spectroscopy, elemental analysis, inductively coupled plasma optical emission spectrometry, zeta potential measurements, scanning electron microscopy, determination of COOH and CHO groups content, and antimicrobial activity under dynamic contact conditions. Influence of pretreatments on NCS and NCS + Zn adsorption, chemical, electrokinetic, and antibacterial properties as well as morphology, and...