First Record and Molecular Identification of Amantia Manginiana in Jordan (original) (raw)
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Lahore Garrison University Journal of Life Sciences, 2020
Swat valley is Himalayan moist temperate forest of Pakistan. It is also called mini Switzerland of Pakistan. This area has diverse flora. A wide diversity of mushrooms is present over here but number of species explored is very small. In the present study, a species of Amanita was collected during field tour and identified on the basis of morpho-anatomical and molecular markers which confirmed its identification as Amanita flavipes. BLAST analysis of this species has been re-investigated from new locality swat and is being reported first time from this area.
Nucleotide Sequencing and Identification of Some Wild Mushrooms
The Scientific World Journal, 2013
The rDNA-ITS (Ribosomal DNA Internal Transcribed Spacers) fragment of the genomic DNA of 8 wild edible mushrooms (collected from Eastern Chota Nagpur Plateau of West Bengal, India) was amplified using ITS1 (Internal Transcribed Spacers 1) and ITS2 primers and subjected to nucleotide sequence determination for identification of mushrooms as mentioned. The sequences were aligned using ClustalW software program. The aligned sequences revealed identity (homology percentage from GenBank data base) ofAmanita hemibapha[CN (Chota Nagpur) 1, % identity 99 (JX844716.1)],Amanitasp. [CN 2, % identity 98 (JX844763.1)],Astraeus hygrometricus[CN 3, % identity 87 (FJ536664.1)],Termitomycessp. [CN 4, % identity 90 (JF746992.1)],Termitomycessp. [CN 5, % identity 99 (GU001667.1)],T. microcarpus[CN 6, % identity 82 (EF421077.1)],Termitomycessp. [CN 7, % identity 76 (JF746993.1)], andVolvariella volvacea[CN 8, % identity 100 (JN086680.1)]. Although out of 8 mushrooms 4 could be identified up to species ...
Survey and Identification of Wild Mushrooms in Eastern Region of Libya
Mediterranean Journal of Basic and Applied Sciences (MJBAS), 2021
Mushrooms are a large and important class of higher Basidiomycetes fungi. Mushrooms are fleshy, sometimes tough, umbrella like shape. Some mushrooms types are edible whils, others are nonedible or poisonous called toad stool. Survey method have been carried out in eastern part of Libya, starting from Benghazi west until Shahat east city. The total regions are 10 (Sidi Khalifa, Alhmada, Albakour, Farzogha, Almarij, Wadi alkouf, Alwardia, Massa, Alwasitta and Shahat). 12 different mushroom samples were collected in three months’ time. All samples are immediately kept in sterile plastic bags and brought to mycology laboratory. Classification and identification have been done by using Macroscopic characters specific books and references. Essential criteria and characters have been followed for identification: Structure of the fruit body, shape, size and colour, cap shape, Stalk, Gills and Teethes or Tubes are the first major key of identification. 9 mushrooms species have been identification only from 12 samples collected as following: Boletus erythropus, Russula cyanoxantha, Amanita phalloides, Amanita fulva, Mycena Pura, Agaricu ssilvaticus, Agaricus silvicola, Agaricus campestris, Coprinus plicatilis.
Molecular identification and artificial cultivation of a wild isolate of oyster mushroom in Albania
Italian Journal of Agronomy, 2016
Basidiomata of a wild mushroom macroscopically recognised as <em>Pleurotus ostreatus</em> were observed on an oak trunk in a mixed wood of northern Albania. Pure cultures of the fungus were then obtained on potato-dextrose-agar medium. Molecular analyses of genomic DNA of the fungus confirmed its identification. The rDNA ITS region nucleotide sequence of the studied <em>Pleurotacea</em> matched at 99% those of two <em>P. ostreatus</em> strains already present in NCBI GenBank database. The rDNA ITS nucelotide sequences of two pure cultures of the Albanian <em>P. ostreatus</em> were deposited in EMBL database under the accession numbers LN849458 and LN849459. One of the fungus isolates was subsequently cultivated under protected and semi-natural conditions. Productivity and biological efficiency of the Albanian <em>P. ostreatus</em> ranged from about 10% to 16% and from 33 to 53.33%, respectively. This seems to be the first r...
AMB Express
Identification of fungal species based on morphological characteristics is tedious, complex, prone to errors, and thus cannot be completely relied upon. In this study, internal transcribed spacers (ITS 1 and 4)-polymerase chain reaction was employed to amplify DNA of 19 mushroom isolates collected at Environmental Pollution Science and Technology farm, Ilesa, Southwest Nigeria. The PCR amplification of ITS1 and 4 of the mushrooms isolates yielded approximately 850 bp. Amplicons obtained were sequenced and identified using BLASTn in the NCBI. The BLASTn results revealed that Termitomyces aurantiacus (3), Tricholoma matsutake , Tricholoma robustum (2), P. ostreatus (4), Schizophyllum commune (1) and Pleurotus pulmonarius (1) were fully represented. Only Tricholoma matsutake (KT273371), Pleurotus pulmonarius (KY962469) and Tricholoma matsutake (AF438605) had 100% similarity with reference strain. However, the phylogenetic analysis of the isolates showed low genetic relatedness with reference strains. This study revealed the novelty of the mushroom strains and thus advocating the need for strict conservation measures and further investigations on their potential benefits to mankind. which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
WORLD JOURNAL OF BIOLOGY AND BIOTECHNOLOGY Amanita pseudovaginata from Pakistan
Amanita pseudovaginata of Amanita subgenus Amanita sect. Vaginatae was found associated with Quercus spp. forests during a survey of macrofungi from oaks forests of Pakistan. The fruiting body was characterized morphoanatomically as well as by molecular analysis. The identification of the fungal symbiont as Amanita pseudovaginata was confirmed by Internal Transcribed Spacer Region (ITS) sequences. Sporocarps were matched with published data available from Russia and China. Phylogenetic analyses and morphological descriptions are provided. This represents the first report of this species in Pakistan.
MSP-PCR and RAPD molecular biomarkers to characterizeAmanita ponderosa mushrooms
Annals of …, 2009
Amanita ponderosa is a specie of wild edible mushrooms growing spontaneously in some Mediterranean microclimates, namely in Alentejo and Andaluzia, in the Iberian Peninsula. The nutritional values of these fungi make them highly exportable. Due to the wide diversity of mushrooms in nature, it is essential to differentiate and to identify the various edible species. RAPD markers have been used as a valuable tool to distinguish the different genotypes, although this method has not yet been used to Amanita ponderosa. Two methods were used to establish different genetic fingerprinting patterns of edible mushrooms. Samples of Amanita ponderosa were collected in six different regions of the southwest of the Iberian Peninsula and compared by RAPD-PCR and MSP-PCR. Additionally, to compare molecular profiles with others genera of edible mushrooms, three species of Basidiomycetes (Pleurotus ostreatus, Lactarius deliciosus and Coriolus versicolor) and an Ascomycete were used. Results showed that some molecular markers discriminate among an Ascomycete from Basidiomycetes (Amanita ponderosa, Pleurotus ostreatus, Lactarius deliciosus and Coriolus versicolor) and discriminate among the different genera within basidiomycetes, as it is expected. Moreover, OPF-6, OPG-2, OPG3 and M13 primes allowed to unravel a level of genetic polymorphism within Amanita ponderosa mushrooms collected from different geographic origin.
The first and most important step of molecular techniques is to isolate the high quality and standard quantity of DNA. The DNA extracted using the recommended method could successfully amplify the regions of interest and demonstrated reliable results can be applied in other molecular assays. Moreover, the designed primers of ITS1-UM2 and ITS4-UM2 were perfectly matched with the species of Basidiomycetes, can be used in phylogenetic studies of other mushrooms. We here evaluated the quality and quantity of DNA using a spectrophotometer, showed reliable OD 260/280 , and concentration. The protocol is efficient, rapid, low-cost, and simple, needs low amount of sample, and requires minimum facilities. The standard yield in addition to the high quality of DNA will enable mycologists to establish molecular techniques easier. In the current study, the constructed phylogenetic tree based on the obtained sequences of Internal Transcribed Spacer (ITS) I and II regions distinctly classified the examined material.
Molecular Identification of Some Ghanaian Mushrooms Using Internal Transcribed Spacer Regions
Molecular Biology
Mushooms have recently attracted attention and are exploited for food and medicinal purposes. Accurate identification of mushrooms is key in utilizing them for the benefit of humans. However, morphological identification of mushrooms is time consuming, tedious and may be prone to error. DNA markers are quick and reliable tools that are useful in mushroom taxonomy. Thus this study confirmed the identity of six Ghanaian mushrooms using the internal transcribed spacer (ITS) sequences. The ribosomal DNA-ITS fragments of genomic DNA of six wild mushrooms were amplified using ITS1 and ITS4 primers. The amplicons were sequenced and data assembled and analyzed using Bio Edit. Basic Local Alignment Search Tool (BLAST) search was carried out using the National Center for Biotechnology Information (NCBI) database. The data obtained from the sequence alignment were used to plot a phylogenetic tree using the Neighbor-Joining method in Molecular Evolutionary Genetics Analysis (MEGA). The nucleotide sequences of the six mushrooms blasted against sequences from GenBank data base revealed that Volvariella volvacea, Trametes elegans,