Effect of Actinomycin D on the Expression of Nuclear Antigen SS-B/La (original) (raw)

Abstract

sparkles

AI

The study investigates the impact of actinomycin D on the expression of nuclear autoantigen SS-B/La. Findings indicate that low concentrations of actinomycin D can significantly reduce the presence of SS-B/La in the nucleolus and the synthesis of associated RNAs, suggesting that actinomycin D inhibits the transcription of messenger RNA required for SS-B/La expression. The implications of these findings are relevant for understanding the mechanisms underlying autoimmune diseases where SS-B/La plays a critical role.

Loading...

Loading Preview

Sorry, preview is currently unavailable. You can download the paper by clicking the button above.

References (19)

  1. Tan EM: Autoimmunity to nuclear antigens, The Cell Nucleus, vol 7. Edited by H Busch. New York, Academic Press, 1979, pp 457- 477.
  2. Tan EM: Autoantibodies to nuclear antigens. Their immunobiology and medicine. Adv Immunol 33:167-240, 1982
  3. Deng JS, Takasaki Y, Tan EM: Nonhistone nuclear antigens reactive with autoantibodies. Immunofluorescent studies of dis- tribution in synchronized cells. J Cell Bioi 91:654-660, 1981
  4. Takasaki Y, Deng JS, Tan EM: A nuclear antigen associated with cell proliferation and blast transformation. Its distribution in synchronized cells. J Exp Med 154:1899-1909, 1981
  5. Moroi Y, Hartmen AL, Nakane PK, Tan EM: Distribution of kinetochore (centromere) antigen in mammalian cell nuclei. J Cell Bioi 90:254-259, 1981
  6. Lerner MR, Steitz JA: Antibodies to small nuclear RNAs com- plexed with proteins are produced by patients with systemic lupus erythematosus. Proc Nat! Acad Sci USA 76:5495-5499, 1979
  7. Lerner MR, Boyle JA, Hardin JA, Steitz JA: Two novel classes of small ribonucleoproteins detected by antibodies associated with lupus erythematosus. Science 211:400-402, 1981
  8. Lerner MR, Andrews NC, Miller G, Steitz JA: Two small RNAs encoded by Epstein-Barr virus and complexed with protein are precipitated by antibodies from patients with systemic lupus erythematosus. Proc Nat! Acad Sci USA 78:805-809, 1981
  9. Lerner MR, Boyle JA, Mount SM, Wolin SL, Steitz JA: Are snRNPs involved in splicing? Nature 283:220-224, 1980
  10. Rosa MD, Gottlieb E, Lerner MR, Steitz JA: Striki ng similarities are exhibited by two small Epstein-Barr virus-encoded ri- bonucleic acids and the adenovirus-associated ribonucleic acids VA I and VA II. Mol Cell Bioi 1:785-796, 1981
  11. Hendrick JP, Wolin SL, Rinke J, Lerner MR, Steitz JA: Ro small cytoplasm ic ribonucleoproteins are a subclass of La ribonucleo- proteins: further characterization of the Ro and La small ribo- nucleoproteins from uninfected mammalian cells. Mol Cell Bioi 1:1138-1149, 1981
  12. Rinke J, Steitz JA: Precursor molecules of both human 5S ribo- somal RNA and transfer RNAs are bound by a cellular protein reactive with anti-La lupus antibodies. Cell 29:149-159, 1982
  13. Lindell TJ, O'Malley AF, Puglisi B: Inhibition of nucleoplasm transc ription and the translation of rapidly labeled nuclear pro- teins by low concentration of actinomycin D in vivo. Proposed role of messenger RNA in ribosomal RNA transcription. Bio- chemistry 17:1154-1160, 1978
  14. Warner JR: In the absence of ribosomal RNA synt hesis the ribo- somal proteins of HeLa cells are synthesized normally and degraded rapidly. J Mol Bioi 115:315-333, 1977
  15. Perry RP: Selective effects of actinomycin D on the intracellular . distribution of RNA synthesis in tissue culture cells. Exp Cell Res 29:400-406, 1963
  16. Lindell TJ: Evidence for an extranucleolar mechanism of actino- mycin D action. Nature 263:347-350, 1976
  17. Epifanova 01, Abuladze MK, Zosimovskaya AI: Effects of low concentration of actinomycin D on the inhibition of DNA syn- thesis in rapidly proliferating and stimulated cell cultures. Exp Cell Res 92:23-30, 1975
  18. Alspaugh MA, Tala! N, Tan EM: Differentiation and characteri- zation of autoantibodies and their antigens in Sjogren's syn- drome. Arthritis Rheum 19:216-222, 1976
  19. Perry RP, Kelly DE: Persistent synthesis of 5S RNA when pro- duction of 28 S and 18 S ribosomal RNA is inhibited by low doses of actinomycin D. J Cell Physiol 72:235-246, 1969