Native Bacteria Isolated from Phosphate Deposits Reveal Efficient Metal Biosorption and Adhesion to Ore Particles (original) (raw)
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Scientific Reports, 2019
Chemical reagents used in traditional mineral processing can be toxic and hazardous for the environment. Therefore, the use of biotechnological methods is becoming increasingly important. Great hopes are being placed in the use of microorganisms for bio-beneficiation of raw materials. However, assessment of adhesion abilities of bacteria onto minerals surface as well as biosorption of metals are essential steps before designing final process of each ore beneficiation. The main aim of this work was an investigation of biosorption of Cd and Mg, as well as adhesion abilities of five microorganism species with minerals included in the natural mixture of phosphate ore form Djebel Onk, Algeria. The ore, due to its unique composition, created conditions for adhesion of all five tested microbial strains onto apatite surface during incubation at pH 3. Moreover, Rhodococcus erythropolis CD 130, Pseudomonas fluorescens and Escherichia coli adhered distinctly onto apatite surface during incubat...
Mg and Cd biosorption by native bacteria form Djebel Onk mine (Algeria)
2021
Mining and processing of phosphate ore are one of the most important branches of the economy in developing countries, including Algeria. However, this activity can negatively in uence the environment, as huge amounts of waste, which contains dangerous metals, are released during ore processing. Therefore, in line with environmental needs, conventional methods of ore bene ciation should be gradually replaced with safe, biotechnological methods that involve (among others) the biosorption phenomenon. This work aimed at the investigation of biosorption abilities of native microorganisms, isolated from Djebel Onk ore (Kef Essnoun region, Algeria). Examined bacterial strains differed in their e ciency of metal accumulation. In the vast majority of native bacteria, the content of Cd or Mg found was higher than in a reference B. subtilis strain. Incubation of phosphate ore with selected bacterial strains (for 20 minutes and at different pH) signi cantly increased the recovery of Mg and Cd. Thus, we showed that biosorption could be much more effective for native bacteria isolated from the speci c substrate. We have shown that Bacillus sp. HK4 strain can be used to remove various metals over a wide pH range, and it can be considered in the development of eco-friendly measures to clean ore and postotation waste.
Native Bacteria from Djebel Onk Mine (Algeria) Exhibit Selective Adhesion onto Phosphate Ore
Recent Advances in Environmental Science from the Euro-Mediterranean and Surrounding Regions (2nd Edition), 2021
The main aim of the present work was the investigation of the adhesion abilities of selected native microorganisms with phosphate ore particles-natural components of raw material form Djebel Onk, Kef Essnoun region, Algeria. The adhesion/sedimentation level was assessed based on optical density (OD). Microorganisms were marked with quantum dots (CdTe QD; PlasmaChem GmbH), incubated with phosphate ore, and then the particle surface was analyzed using a confocal laser scanning microscope. The highest adhesion onto ore was found for: HK2 3K strain at pH 7, B. subtilis at pH 4, and HKR2G1D(a) strain at a wide range of pH. The use of quantum dots for imaging microorganisms revealed their selectivity in adhesion to particles included in phosphate ore from Djebel Onk. Native bacteria with high adhesion properties (HK2 3K) have priority in settling particle surfaces. This is an effect of a long-lasting selection of microorganisms to these specific conditions in the Kef Essnoun region. The assessment of microorganism-particle complexes stability and their floatability will be continued.
An Overview: Application of Microorganisms in Bio-Mining of Metals (Review Article)
International Journal of Pharmacy and Biological Sciences, 2021
Biomining of copper from ores was started during ancient era without even knowing the role of microorganisms in the process. The ever-increasing demand of metals, declination of natural resources, huge reserves of low-grade metallic ores and generation of massive amount of metallic wastes from mining and beneficiation process has led to the evolution and commercial scale adoption of biomining. The ability of microorganisms to bioamine metals depends on redox reactions, organic or inorganic acid formation and the release of complexing agents. Redox reaction is the key step of biomining process which is based either on direct or indirect bioleaching. The main difference between the two mechanisms is the direct contact between the microorganisms and the reduced minerals. Apart from the economic benefits, biomining also reduces the problem of acid mine drainage (AMD). Biomining is successfully utilized in metal recovery from low grade ores, mine tailings, mine wastes, municipal solid wa...
Comparative Study of Biosorption of Metallic Cations by Different Bacteria
SOUTHERN BRAZILIAN JOURNAL OF CHEMISTRY, 1998
Certain species of microorganisms have been found to accumulate surprisingly large quantities of important metals, involved in the toxicity provoked by human activities (Cd, Pb, Hg) and metals of economic values (Ag, Au). Microbiological methods are applied to large-scale recovery or removal of metallic ions from aqueous solutions. These applications involved the removal of heavy metals from sewage sludge, industrial effluents, and mine or wastewaters. This process has been developed using immobilized extracellular or cellular ligands or more simple chemical models based upon them. The uptake of metal ions on the cell surface and their translocations into the cell are well-known natural processes. These adsorption processes could be expressed using Langmuir isotherms. Fe2+ and Mn2+ appeared to .be the most effective cations for adsorption by Bacillus subtilis, Pseudomonas aeruginosa, even by Saccharomyces cerevisiae, while the Zn2+ cation, in spite of a great value of maximum adsorp...
2018
Finding a cleaner, environmentally friendly and cost-effective way of metal and mineral extraction has a great importance in today’s world. Using microorganisms in bio-leaching and bio-oxidation process is of great value. From Archaea to bacteria and fungi, microorganisms can play an important role in extraction of metals from mine drainage and un-accessible sources, both in aquatic and terrestrial environments. Optimization of environmental factors such as the temperature, pH and substrate concentration is crucially important to access the optimum extraction of selected metals from an ore or mine drainage. The present paper will review the bio-leaching and bio-oxidation process of minerals with emphasis on the most well-known species of bacterial communities of such ability, through the literature.
Bioprocessing of Natural Phosphate Ore with Staphylococcus Aureus Bacteria
Rudarsko-geološko-naftni zbornik
Phosphate ores are in high demand around the world because they are the primary raw materials used in the manufacturing of phosphatic fertilizers and other chemicals. Since the grade of the ore is gradually declining, it is becoming economically viable to mine and beneficiate numerous lower-grade deposits, and a significant number of precious minerals are discarded due to the inadequacy of new technological advances. Thus, biological processes are becoming more appealing in mineral processing due to their lower operating costs and potential applications to beneficiate low-grade complex ores through the interaction of bacteria and mineral surfaces, resulting in surface modification and mineral separation via bio-flotation. Staphylococcus aureus was supplied by the mineral bioprocessing lab, CMRDI. Bacterial adhesion measurements revealed a higher affinity for apatite than quartz. A binary mixture containing 12.5% P2O5 and 42.5% SiO2 yielded a concentrate containing 20.15% P2O5 and 33...
Mine tailings sites have caused severe damage to the ecosystems through production of a large amount of heavy metals. Ecological remediation of metal polluted sites by means of plants and microbes has received much attention around the world. Phytoremediation of metal contaminated sites in association with phosphate-solubilizing bacteria is considered to be a suitable option for overcoming the metal stress on the environment. The aim of the present study was to isolate and characterize phosphate solubilizing bacteria from rhizospheric soil of plants growing in tailing dam of Zawar mines, Udaipur and to determine their zinc tolerance ability. A total of 265 isolates were recovered on Pikovskaya’s agar out of which 96 isolates were found to solubilize phosphate. The solubilizing efficiency of the isolates ranged between 6.25 to 350. A total of 38 isolates out of 96 were able to grow on nutrient agar supplemented with 1 mg/ml (minimum concentration) of zinc sulphate heptahydrate (ZnSO4.7H2O). Minimum inhibitory concentration of ZnSO4.7H2O for the 38 isolates ranged between 2 to 24 mg/ml. On the basis of remarkable phosphate solubilization and zinc tolerance ability five isolates (PSB 11, PSB 16, PSB 51, PSB 55 and PSB 91) were selected for further characterization. On the basis of biochemical profiling and partial sequence analysis of respective 16S rRNA genes, the five isolates were found to belong to two different genera i.e., Pseudomonas and Cronobacter. The phosphate solubilizing activity of the isolates along with zinc tolerance ability may provide a new approach for bioremediation of metal-polluted soils.
Process Biochemistry, 2004
The use of biological materials for effective removal and recovery of heavy metals from contaminated wastewaters has emerged as a potential alternative method to conventional treatment techniques. The aim of this paper was the laboratory study of biosorption of toxic metals from aqueous solution by the application of microorganisms (Bacillus laterosporus or Bacillus licheniformis), isolated from polluted (metal-laden) soil. Microorganisms have a high surface area-to-volume ratio, because of their small size and therefore, they can provide a large contact interface, which would interact with metals from the surrounding environment. Microbial metal accumulation has received much attention during recent years, due to the potential use of microorganisms for treatment of metal-polluted water or wastewater streams. Two toxic metals were selected as typical examples: a cation (cadmium) and an oxyanion (hexavalent chromium, and promising results were obtained, under optimized conditions.