Alcohol Intake Investigation of Adult Rats Based on Sperm Parameters (original) (raw)
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International Medical Journal Malaysia, 2013
Introduction: The goal of the present study was to examine the effect of alcohol consumption on sperm count and motility and the morphological changes in the seminiferous tubules of parent mice and their offspring. Methods: Animals were divided into two groups, Group 1 (alcohol group) of twelve male and twelve female mice, were given a daily dose of (3 g/kg body weight as 25%, v/v) ethanol by gastric gavage for four and eight weeks. Group 2 (control group) also of twelve male and twelve female mice; received normal access of food and water. After four weeks of treatment, the males and females in each group were allowed to mate, and ethanol treatment continued for up to another four weeks. Twelve male offspring from group 1 and twelve male offspring from group 2 were selected randomly and allowed to become mature. Male parent mice were killed at the 4 th and 8 th weeks of treatment, and their male offsprings were killed when they reached maturity age. Results: Physiological examination of the sperm solution showed that there was a significant decrease in sperm count and motility after 4 and 8 weeks of ethanol treatment in parent male mice, but this decrease was not significant in their adult offspring. Furthermore, histological investigations indicated testicular lesions in the parent male mice and their adult male offspring. Conclusion: Alcohol abuse has deleterious effects on the testes structure and on the sperm count and motility of the epididymal spermatozoa of both parent mice and their offspring.
Direct effect of alcohol on the motility and morphology of human spermatozoa
Andrologia, 1999
Excessive alcohol consumption has been associated with impaired reproductive function by causing the inhibition of penile tumescence and ejaculatory capability. Alcohol intoxication has also been implicated in impaired spermatogenesis ancl an increase in sperm structural anomalies. The aim of this study was to determine the direct effects of alcohol on sperm motility and morpholog;\-in vitro.
Effects of ethanol ingestion on sperm monosaccharides and fertility
IUBMB Life, 1999
Chronic alcohol abuse is often associated with reproductive disorders. Sperm monosaccharides play an indispensable role in sperm-egg interactions and fertilization. Ethanol (3 g/kg body weight as 25%, v/v) was given by gastric intubation twice daily for 30 days while in another group, rats which had been treated with ethanol were withdrawn from treatment for a further period of 30 days, in order to assess the reversibility of the ethanol-induced effects. Epididymal ethanol content, sperm monosaccharides and the fertility of ethanol treated and ethanol withdrawn rats were assessed. Ethanol ingestion caused a significant decrease in sperm monosaccharides suggesting defective glycosylation of sperm surface proteins. Sperm monosaccharides and fertility were returned to normal following the withdrawal of ethanol. Ethanol-induced changes in sperm monosaccharides may be one of the reasons for the reduced fertility of ethanol treated rats.
Effects of concurrent chronic administration of alcohol and nicotine on rat sperm parameters
Andrologia, 2012
The prevalence of cigarette and alcohol consumption is high among young adult males during the reproductive period. The current study aimed to evaluate the impact of concurrent chronic administration of nicotine and ethanol on the quality of sperm in the rat. Fifty healthy Wistar male rats were randomly divided into five groups (n = 10) and were given the following for a period of 50 days: ethanol (E), nicotine (N), ethanol and nicotine (E/N); the control group (C) and an intact (I) group. Body weight as well as the weight, volume and dimensions of the testes and the weight of the cauda epididymidis and vas deference were measured. The concentration, motility, viability and membrane integrity of sperm were also assessed. There were no significant differences between body weight and all testis parameters including weight, volume and dimensions. The concentration and motility of sperm in the E/N group was significantly reduced compared with the control group (P < 0.01). Nevertheless, only a marginally significant decrease in sperm viability was found in the E/N group compared with the control group. The study indicates that concurrent chronic administration of ethanol and nicotine may disturb male reproductive function.
2021
Challenges associated with habitual intake of alcohol including health, social, psychological and especially reproductive health needs urgent attention. This study aimed to determine the spermatotoxic effect of selected traditional alcoholic beverages in rats. A total of 30 normal male Spaque dawley strain albino rats weighing 180-220g, divided into 5 groups of 6 rats in each were administered with 10ml/kg p.o each of pito, goskolo and ogogoro, goskolo respectively and 0.5ml/kg normal saline for a period of 21 days. Sperm samples were harvested from the left caudal portion epididymis assayed for sperm motility, sperm morphology and sperm count after which histological examination was carried out on the testes. Results showed that active, sluggish and dead sperm cells were goskolo>pito>burukutu>control>ogogoro, ogogoro> burukutu> control>goskolo>pito and control>pito>ogogoro>burukutu>goskolo respectively. For morphology of sperm cells, it was gosko...
Murine sperm capacitation, oocyte penetration and decondensation following moderate alcohol intake
Reproduction (Cambridge, England), 2018
Male chronic alcohol abuse causes testicular failure and infertility. We analyzed the effects of moderate sub-chronic alcohol intake on sperm morphology, capacitation, fertilization and sperm head decondensation. CF-1 male mice were administered 15% ethanol in drinking water for 15 days; control mice received ethanol-free water. Similar patterns of tyrosine phosphorylation were observed in capacitated spermatozoa of control and treated males. Percentage of hyperactivation (H) and spontaneous (SAR) and progesterone-induced (IAR) acrosome reaction significantly decreased at 120 and 150 min of capacitation in treated males compared to controls (H: 14.1 ± 2.5 vs 23.7 ± 2.6, < 0.05; SAR-T120 min: 17.9 ± 2.5 vs 32.9 ± 4.1, < 0.01; IAR-150 min: 43.3 ± 3.5 vs 73.1 ± 1.1, < 0.001, = 6). During fertilization (2.5, 3.5 and 4.5 h post-insemination), there was an increased percentage of fertilized oocytes (with a decondensed sperm head and one or two pronuclei) in treated males ( &l...
Alcohol and the male reproductive system
Alcohol Research Health the Journal of the National Institute on Alcohol Abuse and Alcoholism, 2001
: hypothalamic-pituitary-gonadal axis; reproductive effects of AODU (alcohol and other drug use); male; reproductive system; testicles; nitric oxide; oxidation; ethanol-to-acetaldehyde metabolism; apoptosis; luteinizing hormone-releasing hormone; fertility; opioids MARY ANN EMANUELE, M.D., is a professor in
Neurotoxicology and Teratology, 1992
Comparison of two weeks versus one week of prenatal ethanol exposure in the rat on gonadal organ weights, sperm count, and onset of puberty. NEUROTOXICOL TERATOL 14(5) 351-358, 1992.--Sprague-Dawley dams from Harlan Ind. (Indianapolis, IN) were administered a fortified ethanol fiquld diet containing 35"/e ethanol derived calories for two weeks (E-2) beginning on day 7 or one week (E-l) beginning on day 13 of gestation and continuing through parturition. Control dams were pair-fed an isocaloric fiquid diet containing no ethanol during these periods or remained on lab chow and water. E-2 dams consumed an average of 13.52 g ethanol/kg bwt during the first week of exposure (days 8-14) and 12.50 g ethanol/kg bwt the second week (days 14-20). E-I dams consumed significantly less than E-2 dams during the second week (9.75 g/kg; p < 0.0001). Although the lower consumption in E-I dams led to a significant decrease in maternal weight gained during the few days of pregnancy compared to E-2 dams, birthweights of E-I offspring were significantly heavier than those of E-2 offspring (p < 0.05). No effect of ethanol was detected on anogenital distance at birth in either sex. Puberty was delayed in female offspring of both E-1 and E-2 dams (p < 0.01) as measured by age of vaginal opening. These data suggest that the primary teratogenic actions of ethanol in the rat on fetal growth, as well as delayed puberty in females, occur in the last week of gestation. ! n adult E-2 males, testis weight was significantly heavier than all other groups when indexed to body weight. No effect of prenatal ethanol exposure was observed on the indexed weights of prostate, epididymis, or seminal vesicles. Adult plasma testosterone levels and sperm counts also were not significantly influenced by prenatal ethanol exposure. Overall, we failed to observe long-term effects of ethanol on the reproductive system of male Sprague-Dawley rats as reported in other strains.
Toxicology, 2015
Chronic consumption of ethanol causes morphological and physiological changes in the reproductive system of mammals. Vitamin C has an antioxidant role in organisms by neutralizing the ROS (reactive oxygen species) produced by oxidizing agents and this vitamin has an important function in the male reproductive system. The aim of this study was to evaluate whether vitamin C could prevent or attenuate the alterations in the male reproductive system caused by ethanol consumption. To test this hypothesis, male rats were divided into three experimental groups and treated by gavage for 63 days. The ethanol (E) and ethanol + vitamin C (EC) groups received 2 g/kg of ethanol (25% v/v) daily. In addition to ethanol, the EC group received vitamin C at a dose of 100 mg/day, diluted in water. The control group (C) received only the vehicle. On the 64th experimental day, the animals were anesthetized and euthanized, and blood was collected for plasmatic hormonal analysis. The testis, epididymis, vas deferens, and seminal vesicles were removed and weighed. Sperm from the vas deferens was submitted to morphological and motility analysis. The testis and epididymis were used for oxidative stress and histopathological analysis, sperm count, morphometric analysis of the testis, and stereological analysis of the epididymis. The results showed that vitamin C has a protective effect in the testes of adult male rats, entirely normalizing the parameters of sperm count, spermatogenesis kinetics, lipid peroxidation levels, and sperm motility, as well as partially normalizing the histopathological damage in the testis, epididymis, and sperm morphology. Thus, we concluded that lipid peroxidation is a major mechanism by which ethanol affects the testes and sperm, whereas no plasmatic testosterone alterations were found.
Alcohol's Effects on Male Reproduction
Alcohol Health and Research World, 1998
: AODE (alcohol and other drug effects); hypothalamus; pituitary gland; male genitals; reproductive function; testosterone; hormone metabolism; heavy AOD use; cell type; luteinizing hormone; follicle stimulating hormone; gonadotropin RH; secretion; animal model; male; literature review article by Hiller-Sturmhöfel and Bartke, pp. 153-164.) Among other hormones, the hypothalamus produces gonadotropinreleasing hormone (GnRH). GnRH is MARY ANN EMANUELE, M.D., is a professor in the EMANUELE, M.A.; KIRSTEINS, L.; REDA, D.; EMANUELE, N.V.; AND LAWRENCE, A.M. In vitro effect of ethanol exposure on basal and GnRHstimulated LH secretion from pituitary cells. Endocrine Research 15:293-401, 1989. EMANUELE, M.A.; TENTLER, J.J.; REDA, D.; KIRSTEINS, L.; EMANUELE, N.V.; AND LAWRENCE, A.M. The effect of in vitro ethanol exposure on 201 LHRH release from perfused rat hypothalami. Endocrine Research 16:313-321, 1990. EMANUELE, M.A.; TENTLER, J.; EMANUELE, N.V.; AND KELLEY, M.R. In vivo effects of acute EtOH on rat α and β luteinizing hormone gene expression. Alcohol 8:345-348, 1991.