Ag2[Fe(CN)5NO] Nanoparticles Exhibit Antibacterial Activity and Wound Healing Properties (original) (raw)
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Antibiotics
Green nanotechnology has significant applications in various biomedical science fields. In this study, green-synthesized silver nanoparticles, prepared by using Catharanthus roseus and Azadirachta indica extracts, were characterized using UV–Vis spectroscopy, dynamic light scattering, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. Silver nanoparticles (Ag NPs) synthesized from leaf extracts of C. roseus and A. indica effectively inhibited the growth of multidrug-resistant (MDR) bacteria isolated from patients with septic wound infections. The maximum bacteriolytic activity of the green-synthesized Ag NPs of C. roseus and A. indica against the MDR bacterium K. Pneumoniae was shown by a zone of inhibition of 19 and 16 mm, respectively. C. roseus Ag NPs exhibited more bacteriolytic activity than A. indica Ag NPs in terms of the zone of inhibition. Moreover, these particles were effective in healing wounds in BALB/c mice. Ag NPs of C. roseus and A...
Journal of Nanomaterials, 2022
The biogenic synthesis of silver nanoparticles has recently attracted more attention to counter microbial resistance, which has been one of the medical concerns in the last decade. This research expresses the biogenic synthesis of silver nanoparticles utilizing Ferula assafoetida aqueous extract (Fer@AgNP) as a reducing and capping agent. The total parts of the plant were extracted from an aqueous solution (FerEX) and characterized using GC/MS apparatus. The Fer@AgNP and chemically synthesized silver nanoparticles (AgNPs) were characterized using UV-vis, Fourier transform infrared (FTIR) spectroscopies, field emission-scanning transmission electron microscopy, powder X-ray diffraction analysis, and energy-dispersive X-ray spectroscopy. The impacts of nanoparticles and FerEX were evaluated against four pathogenic bacterial strains, including Staphylococcus aureus, Escherichia coli, Salmonella typhi, and Enterococcus faecalis, using the microdilution method. The biocompatibility of compounds was also evaluated on human cell line L-929 using MTT and human blood cells using the hemolytic assay. The major compounds found in FerEX were sulfur-containing compounds such as butyl disulfides (45.36%) and monoterpenes such as α-pinene (25.66%), β-pinene (16.31%), and ocimene (7.26%). The characterizations of materials confirmed the hexagonal structure of AgNPs. The sizes of cAgNP and Fer@AgNP were about 42.7 nm and 22.5 nm. The antimicrobial activity of Fer@AgNP was considerably developed and reached MIC values ranging from 10 to 50 μg/mL compared to AgNP, which showed MIC values ranging from 50 to 100 μg/mL. The biocompatibility assessment showed that the Fer@AgNP was improved compared to AgNP and had a minimal toxic impact on the normal fibroblast cell line. The Fer@AgNP also indicated outstanding compatibility with human RBCs. The results illustrated that biosynthesized Fer@AgNPs have improved antimicrobial efficacy against Gram-negative and Gram-positive pathogenic bacteria with promising biocompatibility and can be used as potential antibacterial agents.
Biosynthesized silver nanoparticles and their therapeutic applications
Comprehensive Analytical Chemistry, 2021
The human-pathogenic bacteria have become highly resistant to conventional antibiotics; for this reason, a new biosynthesized nanomaterial might be a solution. The culture filtrate of two isolates of Fusarium oxysporum (14, 17) was used in the biosynthesis of nanosilver (AgNPs). The size of the nanoparticles produced by isolate F14 ranged from 19 to 30 nm, whereas the size of those formed via isolate F17 ranged between 16 and 25 nm. Moreover, the produced bio-nanosilver was tested against the human-pathogenic bacteria Proteus vulgaris, Escherichia coli, Staphylococcus aureus, and Klebsiella pneumonia and the outcome results displayed great antibacterial efficacy in a different manner compared with the three different biogenic antibiotics. Collectively, the results depicted that the silver nanoparticles (AgNPs) showed a three and a half times greater activity than the used antibiotics. Differential display reverse transcription-polymerase chain reaction was used to study gene regulation in the treated E. coli (F14) compared with the nontreated ones. Different upregulated and downregulated genes were observed. The cytotoxicity of the produced AgNPs was examined on rats with an average body weight of 200 g each; these animals were grouped into three different groups. The obtained AgNPs showed very low toxicity on the treated rats in comparison to the control group. The physiological parameters, for example, alanine aminotransferase, aspartate transaminase, albumin, creatinine, and urea in the treated animals were changed within to a lower degree compared with those in the nontreated animals. The current study exhibited that AgNPs might be favorable antibacterial agents, especially against multidrug-resistant bacteria.
FEMS Microbiology Letters
A simple and facile way of using biogenic silver nanoparticles (BSNP) (10–20 nm) were fabricated for wound healing acceleration and suppressing wound infections. The BSNP were formulated in the ointment base and conducted the study to accelerate the wound healing process on rat. The BSNP ointment pH 6.8 ± 0.5 lies in normal pH range of the human skin with a good spreadability and diffusibility. The percentage of wound closure rate was highest at day 3 of BSNP ointment treated wound by 22.77 ± 1.60%, while in untreated control 10.99 ± 1.74%, Betadine 14.73 ± 2.36% and Soframycin 18.55 ± 1.37% compared to day 0. The similar pattern was found at day 7 and 11. The antibacterial activity of BSNP was evaluated against wound infection causing bacteria Staphylococcus aureus, Pseudomonas aeruginosa and E. coli by agar diffusion method. The total bacterial counts on wound area were enumerated by colony forming unit method. The lowest number of bacterial counts was found in BSNP treated wound ...
A Review: Silver Nanoparticles in Wound Healing
2021
Wound healing is achieved through a normal biological process in the human body with four precisely and highly programmed phases: Hemostasis, Inflammation, Proliferation, and Restoration. Successful wound healing must occur with the proper sequence of phases and time frame. Interference of many factors like bacterial infection, diabetes, obesity, smoking, and nutrition causing impaired wound healing. This article reviews the recent literature on wound healing ability of antibiotics and metallic nanoparticles. Influence of these factors on repair may lead to better understanding of the combination of antibiotics with different types of metallic nanoparticles to therapeutics that improve wound healing and resolve impaired wounds. Different types of antibiotics were used to release the pressure of infections which obliviously affect the wound healing process. Development of novel and potent bactericidal agents is of great clinical importance due to novel strains of the bacteria and oth...
Journal of Cluster Science, 2020
The development of nano-sized scaffolds with antibacterial properties that mimic the architecture of tissue is one of the challenges in tissue engineering. In this study, polycaprolactone (PCL) and PCL/gelatine (Ge) (70:30) nanofibrous scaffolds were fabricated using a less toxic and common solvent, formic acid and an electrospinning technique. Nanofibrous scaffolds were coated with silver (Ag) in different concentrations of silver nitrate (AgNO 3) aqueous solution (1.25, 2.5, 5, and 10 %) by using dipping method, drying and followed by ultraviolet (UV) photoreduction. The PCL/Ge (70:30) nanofibrous scaffold had an average fibre diameter of 155.60 ± 41.13 nm. Characterization showed that Ag was physically entrapped in both the PCL and PCL/Ge (70:30) nanofibrous scaffolds. Ag ? ions release study was performed and showed much lesser release amount than the maximum toxic concentration of Ag ? ions in human cells. Both scaffolds were non-toxic to cells and demonstrated antibacterial effects towards Grampositive Bacillus cereus (B. cereus) and Gram-negative Escherichia coli (E. coli). The Ag/PCL/Ge (70:30) nanofibrous scaffold has potential for tissue engineering as it can protect wounds from bacterial infection and promote tissue regeneration.
Advances in Nanotechnology towards Development of Silver Nanoparticle-Based Wound-Healing Agents
International Journal of Molecular Sciences
Since antiquity, silver-based therapies have been used in wound healing, wound care and management of infections to provide adequate healing. These therapies are associated with certain limitations, such as toxicity, skin discolouration and bacterial resistance, which have limited their use. As a result, new and innovative wound therapies, or strategies to improve the existing therapies, are sought after. Silver nanoparticles (AgNPs) have shown the potential to circumvent the limitations associated with conventional silver-based therapies as described above. AgNPs are effective against a broad spectrum of microorganisms and are less toxic, effective at lower concentrations and produce no skin discolouration. Furthermore, AgNPs can be decorated or coupled with other healing-promoting materials to provide optimum healing. This review details the history and impact of silver-based therapies leading up to AgNPs and AgNP-based nanoformulations in wound healing. It also highlights the pro...
Development and Experimental Evaluation of Some Silver Nanoparticles with Antimicrobial Potential
Processes
By adjusting the synthesis process, silver nanoparticles (AgNp) of various shapes, sizes, and structures can be obtained, all of which have a substantial impact on the biological effect, notably, the regulation of antibacterial activity in the present circumstances of growing bacterial resistance. Due to their relatively small size, nanoparticles may be disseminated evenly throughout the body of the experimental animal, even at low doses, and exert more potent antibacterial activities. Our research was centered on the synthesis, production, and biological evaluation of antibacterial silver nanoparticles. Using the Turkevich method, we were able to effectively synthesize and characterize nanoscale silver particles, with an average crystallite size of 9.49 nm. We examined their acute toxicity and pharmacokinetic characteristics in rats after administering a single dosage. In addition, we evaluated the biological effect of topical AgNp suspension on the progression of burn-type lesions...
Frontiers in Bioengineering and Biotechnology
Biogenic nanoparticles are the smartest weapons to deal with the multidrug-resistant "superbugs" because of their broad-spectrum antibacterial propensity as well as excellent biocompatibility. The aqueous biogenic silver nanoparticles (Aq-bAgNPs) and ethanolic biogenic silver nanoparticles (Et-bAgNPs) were synthesized using aqueous and ethanolic extracts of Andrographis paniculata stem, respectively, as reducing agents. Electron microscopic images confirmed the synthesis of almost spherical shaped biogenic silver nanoparticles (bAgNPs). The zeta potentials of the nanoparticles were negative and were −22 and −26 mV for Aq-bAgNPs and Et-bAgNPs, respectively. The antibacterial activity of bAgNPs was investigated against seven pathogenic (i.e., enteropathogenic Escherichia coli, Salmonella typhi, Staphylococcus aureus, Vibrio cholerae, Enterococcus faecalis, Hafnia alvei, Acinetobacter baumannii) and three nonpathogenic (i.e., E. coli DH5α, E. coli K12, and Bacillus subtilis) bacteria at different time points (i.e., 12, 16, 20, and 24 h) in a dose-dependent manner (i.e., 20, 40, and 60 µg) through broth dilution assay, disk diffusion assay, CellTox TM Green uptake assay, and trypan blue dye exclusion assay. The lowest minimum inhibitory concentration value for both the bAgNPs was 0.125 µg. Et-bAgNPs showed the highest antibacterial activity against S. aureus at 60 µg after 16 h and the diameter of inhibited zone was 28 mm. Lipid peroxidation assay using all the bacterial strains revealed the formation of malondialdehyde-thiobarbituric acid adduct due to the oxidation of cell membrane fatty acids by bAgNPs. The bAgNPs showed excellent hemocompatibility against human as well as rat red blood cells. Furthermore, there was no significant toxicity observed when the levels of rat serum ALT, AST, γ-GT (i.e., liver function biomarkers), and creatinine (i.e., kidney function biomarker) were determined.
Silver nanoparticles: Antimicrobial activity, cytotoxicity, and synergism with N-acetyl cysteine
The fast progression of nanotechnology has led to novel therapeutic interventions. Antimicrobial activities of silver nanoparticles (Ag NPs) were tested against standard ATCC strains of Staphylococcus aureus (ATCC 9144), Escherichia coli (O157:H7), Pseudomonas aeruginosa (ATCC 27853), and Candida albicans (ATCC 90028) in addition to 60 clinical isolates collected from cancer patients. Antimicrobial activity was tested by disk diffusion method and MIC values for Ag NPs alone and in combination with N-acetylcysteine (NAC) against tested pathogens were determined by broth microdilution method. Ag NPs showed a robust antimicrobial activity against all tested pathogens and NAC substantially enhanced the antimicrobial activity of Ag NPs against all tested pathogens. Synergism between Ag NPs and NAC has been confirmed by checkerboard assay. The effect of Ag NPs on tested pathogens was further scrutinized by Transmission Electron Microscope (TEM) which showed disruption of cell wall in both bacteria and fungi. Ag NPs abrogated the activity of respiratory chain dehydrogenase of all tested pathogens and released muramic acid content from S. aureus in culture. The cytotoxic effect of Ag NPs alone and in combination with NAC was examined using human HepG2 cells and this revealed no cytotoxicity at MIC values of Ag NPs and interestingly, NAC reduced the cytotoxic effect of Ag NPs at concentrations higher than their MIC values. Taken together, Ag NPs have robust antimicrobial activity and NAC substantially enhances their antimicrobial activities against MDR pathogens which would provide a novel safe, effective, and inexpensive therapeutic approach to control the prevalence of MDR pathogens.