Analysis of phenol and tannin in the bark extracts of medicinally important plant Moringa oleifera (Lam.) collected from different regions of Haryana (original) (raw)
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Journal of Pharmacy Research
Comparitive study of polyphenol content in different extracts of Moringa oleifera leaves and fruits.Materials and Methods :Materials required for this study are dried methanolic extract of leaves and fruits of Moringa oleifera, Butanol, Ethyl acetate, Water, folin ciocalteu reagent, Saturated solution of Sodium carbonate. Take 10g of methanolic extract of M. oleifera leaves and fruits, dissolved in 100 ml of water, make partition with ethyl acetate and with butanol. Then concentrate the three extracts separately with the help of rotavapour. Then estimate the content of poly phenols in different extracts of leaves and fruits by using folin ciocalteu reagent method.Results:The poly phenol content of leaves and fruits of M.oleifera present in the different extracts.Conclusion: Total polyphenolic content is more in leaves compared to fruits. In leaves poly phenol content was enriched in butanol fraction and in fruits polyphenol enriched fraction was water.
Turkish Science and Technology Publishing (TURSTEP), 2020
This study was undertaken to estimate the antioxidant activity and total tannin content of Moringa olifera Lam. (Moringa) leaves and find a suitable extraction condition for maximum yield. Two types of solvent and three different extraction times and temperatures were experimented for extraction. Moringa leaves were oven-dried and powdered to determine the antioxidant activity in terms of total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH radical scavenging activity and total tannin content. The maximum TPC value noted in methanol extract, and it ranges from 6.46 ± 0.34 g to 3.91±0.19 g gallic acid equivalent (GAE)/100g dry sample (DM). The FRAP values varied from 211.6 ± 3.75 to 344.13 ± 3.26 mg ascorbic acid equivalent (AAE)/100g DM and 166.67 ± 2.90 to 224.93 ± 1.94 mg AAE/100g DM, for methanol and ethanol extract, respectively. The DPPH radical scavenging activity ranged from 46.32 ± 1.07 to 58.09 ± 0.92% for methanol extract and 56.76 ± 1.48% to 69.72 ± 1.15% for ethanol extract. The total tannin content varied from 6.84 ± 0.05 to 10.22 ± 1.11 mg GAE/100 g dry sample and 6.77 ± 0.08 to 9.23 ± 0.51 mg GAE/ 100g DMfor methanol and ethanol extract, respectively. The highest antioxidant yield for methanol and ethanol extract of Moringa leaves differed for various conditions. Overall, Moringa leaves showed excellent antioxidant properties that can be commercially and domestically used.
2020
Moringa oleifera is a rich source of essential nutrients protein, minerals, vitamins and essential amino acids containing comparatively low amount of antinutritional factors. It is also accomplished by bioactive compounds including phenolic and flavonoid compounds. Qualitative phytochemical analysis of Moringa oleifera leaves was conducted on the biochemistry laboratory of College of Veterinary Science & A.H., Anjora, Durg (C.G). Various extract of M. oleifera leaves powders were prepared in different solvent media viz. aqueous, aqueous methanol, acetone and chloroform extract and analysis were done by various methods. Phytochemical analysis revealed the present of different phytochemicals such as alkaloids, tannins, saponins, flavonoids and glycosides in M. oleifera leaves extracts. Presence of different phytochemicals provide an evidence that M. oleifera leaves may be used as traditional medicine of various diseases however, extensive researches in this areas are needed to prove t...
Jurnal Kimia Sains dan Aplikasi
Moringa (Moringa oleifera Lam) is a medicinal plant that is a source of nutrients. It is rich in protein, fatty acids, minerals, relatively high polyphenol compounds, and has antioxidant activity. This study aims to obtain information about secondary metabolites, total phenol content, total flavonoids, and antioxidant activity of the ethanol extract of Moringa leaves. Total phenol was determined by the Folin-Ciocalteu method, while total flavonoids were determined by the A1C 13 colorimetric method. Whilst the antioxidant activity was determined by the DPPH (2,2diphenyl-i-picrylhydrazyl) method at a wavelength of 517 nm. The results showed that the ethanol extract of Moringa leaves contained flavonoids, phenols, tannins, saponins, alkaloids, and steroids. The total phenol content in the ethanol leaf extract was 63.16 mg GAE / g extract, and the total flavonoid content was 10.477 mg QE / g extract. Moringa leaf ethanol extract has antioxidant activity with an IC 50 of 118.6145 mg / L, classified as having moderate antioxidant activity.
Moringa oleifera (Lam.) is a plant that possesses high nutritional value and has been used in folklore medicine to treat various and socioeconomic benefits. In the present study, we have investigated the phytochemicals constituents of M. oleifera leaves extracts by biochemical method and Fourier transform infrared spectrophotometer (FTIR). The qualitative phytochemical screening indicated presence of alkaloids, triterpenoids, flavonoids, tannins, saponins, glycosides and carbohydrates in the leaves extract. The quantitative analysis of the M. oleifera leaves aqueous extract showed high content of Vitamin C and active compounds such as Polyphenols and Flavonoids which has a strong antioxidant activity. The infrared spectral data revealed the following bonds: O-H, N-H, C=O, NO , C-N, C-H and C-Br that are diagnostic markers of aliphatic as well as aromatic compounds. The methanolic extract of M. oleifera leaves showed the presence of characteristic functional groups like alcohol, hydroxyl, alkane, aldehydes, alkenes groups, nitro compounds, aromatic amines, aliphatic amines and alkyl halides etc. Presence of phytochemicals indicates nutritional and medicinal properties of M. oleifera leaves.
Analysis of total phenolics, tannins and flavonoids from Moringa oleifera seed extract
Moringa oleifera is among the most commonly cultivated plant all over the world. It has high economic impact due to the medicinal and nutritional values. The seeds of M. oleifera also contain various constituents that are useful for therapeutic purposes. The aim of this study was to quantify some important antioxidant compounds of M. oleifera seed extract. Total phenolics (TP), total tannins (TT) and total flavonoids (TF) content were determined by colorimetric method. The results showed that the seed extract contain total phenolics of 10.179 ± 2.894 (mg Gallic acid equivalents / g dry matter) which is higher compared to flavonoid 2.900 ± 0.0002 (mg Quercetin equivalents / g dry matter) and tannic acid of 0.890 ± 0.020 (mg Gallic acid equivalents / g dry matter). Total phenolic content of the seeds is likely to be a key for determining the free radical scavenging and ROS reducing ability of the seeds.
TOTAL POLYPHENOLIC CONTENT AND ANTIOXIDANT PROPERTIES OF MORINGA OLEIFERA LEAF EXTRACTS
The study was carried out to evaluate the relative antioxidant properties and polyphenol contents of partially purified fractions of Moringa oleifera leaves extracts. The total phenolic, total flavonoid, anthocyanin, proanthocyanidine and tannin contents of the crude methanolic extract, aqueous fraction and ethyl acetate fraction were determined using established methods, while the antioxidant properties of the test fractions were evaluated using five in vitro radical scavenging assays: 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, nitric oxide inhibitory assay, lipid peroxidation assay, reductive potential assay, and the ferric reducing ability of plasma (FRAP) assay. The highest radical scavenging effect and polyphenol contents were observed in the ethyl acetate fractions than the other fractions: the order of activity for all the assays was ethyl acetate reaction > crude extract > aqueous extract. The results obtained in the present study indicates that M. oleifera could be a potential source of natural antioxidant and could be applied as a functional food as regard its relatively low tannin content.
Study of Phytochemistry of Moringa Oleifera Leaves
2017
Moringa oleífera commonly called Moringa, is a valuable tree whose fruits, roots and leaves have been advocated for traditional, medicinal and industrial uses. Moringa oleifera is an interesting plant for its use in bioactive compounds. The aim of the present study was to evaluate the chemical composition and nutritional values of dried M. oleifera leaf collected from market of Aurangabad. The phytochemical and nutritional properties of the dried leaf powder of M. oleifera used as nutraceuticals, dietary supplements, functional foods or a source of vegetable in meal preparation. The nutritional and natural products characterization of Moringa conducted in this study show that the leaves of this plant can contribute significantly to the daily recommended allowance needed for many vitamins and mineral needs as well as serve as a rich source of polyphenols, confirming the importance and role that Moringa can play to improve the health and nutrition particularly in malnourished populati...
Moringa oleifera is a small size multipurpose tree of approximately 5 to 10 m in height which is cultivated all over the world. The phytochemical constituents of aqueous and ethanolic stem bark and seed extracts of Moringa oleifera were assessed and compared. The mineral and proximate analyses were carried out on some selected parts of the plant. The result of the qualitative phytochemical constituents showed the presence of tannins, flavonoid, alkaloids, saponins, steroids, terpenoids and cardiac glycoside in both aqueous and ethanolic extracts. Results showed the presence of iron, calcium, potassium and zinc. Proximate analysis in all the investigated plant Original Research Article
Proximate and Phytochemical Composition of Moringa oleifera Leaves at Three Stages of Maturation
Advance Journal of Food Science and Technology, 2011
s: The aim of the study is to determine the proximate and anti-nutrient composition of dried leaves of Moringa oleifera harvested at different stages of the plants maturity. The leaves were harvested at three period of time viz: 10th (early stage), 15th (Mid stage) and 20th (late stage) week after pruning. The result of the proximate analysis revealed that the early stage has the highest carbohydrate content (55.14%). Mid stage recorded the highest moisture (6.3%) while the late stage has highest protein content (28.08%), crude fiber (10.11%), ash (9.25%), fat (2.5) and pH (6.27). The Phytochemicals detected in both aqueous and methanolic extracts of each of the different stage of leaves maturation were the same except for tannin which was present in aqueous extract but was not detected in methanolic extracts. The detected antinutrients are alkaloids, tannins, phenolics, saponins, flavonoids, steroids while phylobatanin and tripertenes were not. All these results indicate that the l...