A comprehensive approach to utilize an agricultural pea peel (Pisum sativum) waste as a potential source for bio-ethanol production (original) (raw)

2015, Romanian Biotechnological Letters

In the present study, a Central Composite Design (CCD) from Response Surface Methodology (RSM) was adopted to produce bio-ethanol using pre-treated pea peel (Pisum sativum). With an aim to achieve optimal yield the concentration of acid load; 1.0, 1.5 and 2.0%, treatment time; 15, 30 and 45 min, and temperature in the range of 100, 110 and 120 °C was selected. Solid P. sativum loading of 10% (w/v) was selected during pre-treatment. After dilute sulfuric acid pre-treatment maximum increase in glucose content (35.97%) of pea peel was achieved at their optimized conditions (1.80%, 30min. and 120°C). Maximal yield of glucose (after 72 h) from pea peel after dilute sulfuric acid pretreatment and enzymatic saccharification (50 °C, pH 4.8 and 120 rpm) by using an enzyme loading of 0.11 g celluclast 1.5 g/L cellulase and 0.05 g cellubiase/g was 17.72 g/L. Fermentation, the last and important step of bio-ethanol production was carried out using fermentative fungal S. bayanus and F. oxysporum...