Lactic Acid Production Vis-à-Vis Biowaste Management Using Lactic Acid Bacteria (original) (raw)
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Lactic acid fermentation within a cascading approach for biowaste treatment
Applied Microbiology and Biotechnology, 2015
Limited availability of resources and increased amounts of waste coupled with an ever-increasing demand for raw materials are typical characteristics of our times. As such, there is an urgent need to accordingly update waste treatment technology. The aim of this study was to determine whether a separate treatment of the liquid and the solid fraction of biowaste could enhance overall efficiency. Liquid fractions obtained from two different separation procedures were fermented at a pH of 5 and uncontrolled pH conditions for 72 h. The fermentation conditions leading to highest lactic acid productivity and yield were evaluated. The substrates gained by both separation procedures showed efficient lactic acid production up to <25 g L −1 . The pH control increased lactic acid concentration by about 27 %. Furthermore, quantitative real-time PCR analyses revealed stronger Lactobacilli growth in these fermentations. As identified via Illumina sequencing Lactobacillus delbrueckii and its closest relatives seemed to drive the fermentation independent of the substrate. These results could help to improve today's resourcing concept by providing a separate treatment of the liquid and solid biowaste fraction.
Production of lactic acid from food wastes
Applied Biochemistry and Biotechnology, 2003
Conversion of food wastes into lactic acid by simultaneous saccharification and fermentation (SSF) was investigated. The process involves saccharification of the starch component in food wastes by a commercial amylolytic enzyme preparation (a mixture of amyloglucosidase, α-amylase, and protease) and fermentation by Lactobacillus delbrueckii. The highest observed overall yield of lactic acid in the SSF was 91% of theoretical. Lactic acid concentration as high as 80 g/L was attainable in 48 h of the SSF. The optimum operating conditions for the maximum productivity were found to be 42°C and pH 6.0. Without supplementation of nitrogen-containing nutrients, the lactic acid yield in the SSF decreased to 60%: 27 g/L of lactic acid from 60 g/L of food waste. The overall performance of the SSF, however, was not significantly affected by the elimination of mineral supplements.
Food waste management-a cheap source of lactic acid produced by Lactobacillus sp
2016
The most common and important chemical compound used in pharmaceutical, cosmetic, chemical and food industry is lactic acid. There have been various attempts made to produce lactic acid efficiently from inexpensive raw materials. The main objective of present study was to produce lactic acid from cheap food waste such as potato peels, orange peels and mango peels as substrate. A total of 35 isolates were screened for Lactobacillus spp. On the basis of temperature and pH optimization, 4 bacterial isolates (5SA, 21SA, 22SA, 32SA) were selected for further study and fermentation. The highest lactic acid production, 12.23 g L was obtained for mango peels where as for orange peels it was 11.98 g L for 21SA isolate. Isolates 5SA and 22SA produced 13.08 g L and 12.54 g L, respectively, lactic acid for potato peels at the 3rd of fermentation. For mixed peel waste, 32SA isolate was able to produce maximum 11.56 g L lactic acid after fermentation at the end of 3rd day. Thus it shown that lact...
Lactic acid is an organic acid produced by Lactobacillus strains and being used in many industries. The present study is aimed to elucidate the ability of lactic acid production with different Lactobacillus strains using various agro waste substrates. These Lactobacillus were isolated from various dairy products. Out of 30 isolates, 15 isolates were identified as Lactobacillus strains by morphological and physiological characterization. These strains were subjected to Solid state fermentation and Submerged fermentation by utilizing the peels of Apple, Cassava, Mosambi, Orange and Pineapple. SSF with Mosambi peel showed a higher yield of lactic acid from Lactobacillus sp LAB 23.Optimum conditions such as temperature, pH, nitrogen sources, mineral salts, inoculum concentration and fermentation time were evaluated for SSF and SmF. In SSF, the optimum culture conditions for the maximum lactic acid production were temperature 37°C, pH 6.5, 2.5% yeast extract as a nitrogen sources, 8% Calicum carbonate, 0.3% magnesium sulphate as mineral salts, 4 % inoculums concentration, substrate concentration 8g, 72 h for fermentation. In SmF, the optimum culture conditions for maximum lactic acid production were temperature 40°C, pH7.5, 3.5% yeast extract as a nitrogen sources, 9% Calicum carbonate, 0.4% magnesium sulphate as mineral salts, 8% inoculums concentration, 10 mL substrate concentration and fermentation time 96 h. The present study suggests that the Mosambi peel is an appropriate agro waste substrates for high yield of lactic acid under optimized conditions. Thus, the Mosambi peel could be used as a good substrate in various industries.
Lactic acid production from Brewer’s Spent Grain by Lactobacillus plantarum ATCC 8014
Journal of Scientific & Industrial Research
Lactic acid is widely used in the food, cosmetic, pharmaceutical, and chemical industries and has received increased attention for use as a monomer for the production of biodegradable poly (lactic acid). It can be produced by either biotechnological fermentation or chemical synthesis, but the former route has received considerable interest recently, due to environmental concerns and the limited nature of petrochemical feedstocks. The objective of this study was to produce lactic acid from Brewery Spent Grain by using lactobacillus plantarum. The production process was carried out in four main stages, such as pretreatment, hydrolysis, fermentation and recovery of lactic acid. Brewery spent grain was dried using oven at 80ºC temperature for 24 hr. Then for the hydrolysis, Box Behnken Design (BBD) was applied to investigate the effect of temperature (115-130ºC), reaction time (25-35min) and acid concentration (1.5-2M) using Design expert® 7 software. RSM was applied to investigate the interaction effect of the hydrolysis process variables and to find the optimum yield of lactic acid from BSG. After hydrolysis process, reducing sugar content of the hydrolyzate was quantified using quantitative benedict reagent solution. Fermentation of the hydrolyzate was performed using 150mL, Lactobacillus plantarum at 35ºC temperature, pH 5.0-5.5 and 200 rpm for 72hrs fermentation time for all samples. After fermentation recovery of lactic acid and purification process was carried out by centrifuging all samples at 5000 rpm for 5 min followed by filtration through 0.2μm paper filter. The concentration of lactic acid was determined by titration of the sample using 4M of sodium hydroxide. Significance of the process variables were analyzed using analysis of variance (ANOVA) and second order polynomial function was fitted to the experimental results. Thus, the influence of all experimental variables, factors and interaction effects on the response was investigated. Hydrolysis temperature, time, sulfuric acid concentration and interaction between reaction temperature and sulfuric acid concentration have significant effect on the yield of lactic acid. RSM optimization yielded the best yield of total reducing sugar and the maximum yield of lactic acid were obtained at 129.75ºC, 32.39 min and 1.89M. Under these condition 53.07% and 26.71% per 150ml of hydrolysate of total reducing sugar and lactic acid was obtained respectively. iii ACKNOWLEDGMENTS I would like to thank the Almighty GOD for giving me the strength and wisdom to successfully complete this thesis for his protection and strength, and an ever present helps in the entire situation and challenge that I face. Moreover, I would like to express my heartfelt appreciation and thank to my Instructor and now this thesis research Advisor Dr.S.Anuradha Jabasingh (Assoc. Professor), for her sustainable and appreciable guidance, tireless advising, for sharing her knowledge, skill, experience and adjustment starting from the development of proposal up to the successful completion of this thesis.
Lactic acid production – producing microorganisms and substrates sources-state of art
Heliyon, 2020
Lactic acid is an organic compound produced via fermentation by different microorganisms that are able to use different carbohydrate sources. Lactic acid bacteria are the main bacteria used to produce lactic acid and among these, Lactobacillus spp. have been showing interesting fermentation capacities. The use of Bacillus spp. revealed good possibilities to reduce the fermentative costs. Interestingly, lactic acid high productivity was achieved by Corynebacterium glutamicum and E. coli, mainly after engineering genetic modification. Fungi, like Rhizopus spp. can metabolize different renewable carbon resources, with advantageously amylolytic properties to produce lactic acid. Additionally, yeasts can tolerate environmental restrictions (for example acidic conditions), being the wildtype low lactic acid producers that have been improved by genetic manipulation. Microalgae and cyanobacteria, as photosynthetic microorganisms can be an alternative lactic acid producer without carbohydrate feed costs. For lactic acid production, it is necessary to have substrates in the fermentation medium. Different carbohydrate sources can be used, from plant waste as molasses, starchy, lignocellulosic materials as agricultural and forestry residues. Dairy waste also can be used by the addition of supplementary components with a nitrogen source.
Biowaste: A Lactobacillus habitat and lactic acid fermentation substrate
Bioresource Technology, 2013
Biowaste is a suitable substrate for lactic acid production. From total bacteria in biowaste 90% are lactic acid bacteria. Lactobacilli: the largest bacterial group in biowaste. Lactobacillus plantarum, Lactobacillus brevis and their closest relatives are the dominating taxa in biowaste.
The current status and future perspectives of lactobionic acid production : a review
Research for Rural Development, 2018
Lactobionic acid is a high value added compound industrially produced through energy intensive chemical synthesis, which uses costly metal catalysts, like gold and platinum. In the next years, biotechnological production of lactobionic acid can be supposed to take the full transition to the manufacturing stage. Productivity of lactobionic acid by microbial production can be affected by various factors-choice of microorganism and its concentration, supply of oxygen, temperature, substrate, cultivation method, pH and aeration rate. The aim was to review research findings for lactobionic acid production as well innovative and efficient technology solutions for self-costs reducing. Whey was recommended as a cheap and suitable substrate for the lactobionic acid production. Whey processing has been advised with Pseudonomas teatrolens in 28 °C and in pH 6 to 7 for yielding the highest productivity. The increasing commercial importance urges the progression of schemes for lactobionic acid biotechnological manufacturing.
Bioutilisation of agro-industrial waste for lactic acid production
International Journal of Food Science & Technology, 2015
The production of biodegradable polymers as alternatives to petroleum-based plastics has gained significant attention in the past years. To this end, polylactic acid (PLA) constitutes a promising alternative, finding various applications from food packaging to pharmaceuticals. Recent studies have shown that d-lactic acid plays a vital role in the production of heat-resistant PLA. At the same time, the utilization of renewable resources is imperative in order to decrease the production cost. This review aims to provide a synopsis of the current state of the art regarding d-lactic acid production via fermentation, focusing on the exploitation of waste and byproduct streams. An overview of potential downstream separation schemes is also given. Additionally, three case studies are presented and discussed, reporting the obtained results utilizing acid whey, coffee mucilage and hydrolysate from rice husks as alternative feedstocks for d-lactic acid production.