In vivo quantification of particle based and gene based MRI reporters in the rodent brain (original) (raw)

2011

Abstract

INTRODUCTION: MRI reporters for in vivo labelling and visualization of endogenous progenitor stem cells (eNPCs) in the adult rodent brain, such as iron oxide particles or reporter genes (e.g. ferritin), result in hypo-intense contrast in high resolution 3D T2*-weighted MR images that can be quantified using suitable image processing tools. We have previously demonstrated that the assessment of lentiviral (LV) and adeno-associated viral (AAV) vector induced expression of the ferritin reporter gene with MRI is confounded by unspecific background contrast at the site-of-injection of the vector in the subventricular zone (SVZ) and around the injection tract (1). However, as the labeled eNPCs migrate away from the SVZ towards the olfactory bulb (OB), LV or AAV mediated MR reporter gene labeling of stem cells still holds potential for neural progenitor cell tracking with MRI. In (2), we quantitatively compared the use of ferritin reporter gene based and micron-sized iron oxide particle (MPIO) based labeling for visualization of the migrated cells in OB using high resolution ex vivo MRI. Here, we demonstrate the potential of this methodology for regional quantification of MRI reporter contrast from in vivo MRI, which could enable longitudinal analysis of regional particle based and reporter gene based MRI contrast changes over time in the same animal.

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