Impact of Physical Chemical Characteristics of Abutment Implant Surfaces on Bacteria Adhesion (original) (raw)
Related papers
Clinical Oral Implants Research, 2013
Objectives: Modifications of titanium (Ti) implant surfaces have a significant effect on early biofilm formation and the outcome of implant procedures. The aim of this study was to examine the role of plasma proteins and electrostatic forces in the adhesion mechanism of oral bacteria to modified Ti surfaces. Materials and methods: Ti discs with three different types of surface modifications, machined, acid-etched, and acid-etched and blasted, were examined for adhesion of oral bacteria: Streptococcus mutans, Porphyromonas gingivalis, and Fusobacterium nucleatum. Following pretreatment of the Ti with ion rich solutions or coating by human serum albumin or fibronectin, bacterial adhesion was examined by scanning electron microscopy and assessed quantitatively by DNA analysis. Ti coating by proteins as well as bacterial adhesion and their interrelationships were further investigated through confocal scanning laser microscopy. Results: Acid-etched and blasted Ti surfaces exhibited significantly higher amounts of bacteria adhesion than the other two surfaces. Calcium was found to serve as a bridging agent in the adhesion process of S. mutans and F. nucleatum to Ti surfaces. Although albumin coating of the Ti reduced the adhesion of S. mutans to all surfaces, it had no influence on the adhesion of P. gingivalis or F. nucleatum. Coating the Ti with fibronectin enhanced P. gingivalis and F. nucleatum adhesion.
2021
Introduction. An important role in the appearance of acute or chronic infections is played by bacterial biofilms that contain several bacterial species and that develop preferentially on inert surfaces, dead tissues and medical devices. Purpose. In this material we tried to make an interesting study, regarding the adhesion capacity of microbial biofilms characteristic of the oral cavity of human subjects on the surface of materials used for industrial scale manufacturing of dental implants: titanium and zirconium dioxide (Zirconia). Material and methods. For this study were used plates of Ti4 and zirconia, on which adhesion of different bacterial strains belonging to the species Enterobacter cloacae, Klebsiella oxytoca and Klebsiella pneumonia were tested. Results and discussions. A rather high microbial load was observed on both types of materials for all types of bacterial strains studied. For certain types of strains, a lower colonization was noted in the case of zirconium dioxid...
Bacterial Adhesion on Commercially Pure Titanium and Zirconium Oxide Disks: An In Vivo Human Study
Journal of Periodontology, 2004
Little is known about the mechanisms of bacterial interaction with implant materials in the oral cavity. A correlation between plaque accumulation and progressive bone loss around implants has been reported. Bacterial adhesion shows a direct positive correlation with surface roughness. Other surface characteristics also seem to be extremely important with regard to plaque formation. Different adhesion affinities of bacteria have been reported for different materials. The aim of this study was to characterize the percentage of surface covered by bacteria on commercially pure titanium and zirconium oxide disks.
The Bacterial Anti-Adhesive Activity of Double-Etched Titanium (DAE) as a Dental Implant Surface
International Journal of Molecular Sciences, 2020
This work aimed to compare the capability of Streptococcus oralis to adhere to a novel surface, double-etched titanium (DAE), in respect to machined and single-etched titanium. The secondary outcome was to establish which topographical features could affect the interaction between the implant surface and bacteria. The samples’ superficial features were characterized using scanning electron microscopy (SEM) and energy dispersive x-ray spectrometry (EDS), and the wetting properties were tested through sessile methods. The novel surface, the double-etched titanium (DAE), was also analyzed with atomic force microscopy (AFM). S. oralis was inoculated on discs previously incubated in saliva, and then the colony-forming units (CFUs), biomass, and cellular viability were measured at 24 and 48h. SEM observation showed that DAE was characterized by higher porosity and Oxygen (%) in the superficial layer and the measurement of the wetting properties showed higher hydrophilicity. AFM confirmed ...
Journal of periodontal & implant science, 2012
Several parameters have been described for determining the success or failure of dental implants. The surface properties of transgingival implant components have had a great impact on the long-term success of dental implants. The purpose of this study was to compare the tendency of two periodontal pathogens to adhere to and colonize zirconia abutments and titanium alloys both in hard surfaces and soft tissues. Twelve patients participated in this study. Three months after implant placement, the abutments were connected. Five weeks following the abutment connections, the abutments were removed, probing depth measurements were recorded, and gingival biopsies were performed. The abutments and gingival biopsies taken from the buccal gingiva were analyzed using real-time polymerase chain reaction to compare the DNA copy numbers of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and total bacteria. The surface free energy of the abutments was calculated using the sessile ...
PubMed, 2011
Purpose: The purpose of this study was to compare zirconium oxide and titanium alloys with respect to their tendency to adhesion and colonization of two periodontal pathogens on both hard surfaces and on soft tissues in vivo. Materials and methods: The present study was designed as a prospective stratified randomized controlled clinical trial. Patients were scheduled to receive two implants with different types of abutments in the posterior mandible. Three months after implant placement, titanium and zirconium abutments were connected. Five weeks after abutment connections, the abutments were removed, probing depth measurements were recorded, and gingival biopsy samples were obtained. Abutments and biopsy specimens were analyzed by reverse-transcriptase polymerase chain reaction to compare the DNA copy numbers of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and total bacteria. The surface free energy of the abutments was calculated by sesile water drop before replacement. Results: No statistically significant differences were found between probing depths or DNA copy numbers of A actinomycetemcomitans, P gingivalis, and total bacteria both for both titanium alloys and zirconium oxide surfaces and the biops specimens obtained from their buccal gingival. With respect to the surface free energy of zirconium and titanium abutments, zirconium abutments showed lower surface free energy than titanium abutments. Conclusion: The results of this study showed that zirconium oxide surfaces have comparable properties to titanium alloy surfaces in their tendency to adhesion and colonization of two periodontal pathogens on both hard surfaces and in soft tissues.
Comparison of In Vitro Biofilm Formation on Titanium and Zirconia Implants
BioMed Research International
Background. Peri-implant diseases are emerging issues in contemporary implant dentistry. As biofilms play a critical role in peri-implant diseases, the characteristic of resisting bacterial adhesion would be ideal for dental implants. The aims of the study were to compare titanium (Ti) and zirconia (Zr) implants regarding the amount of biofilm formation at different time frames and assess the distribution of biofilm on different aspects of dental implants. Methods. Biofilm was developed on Ti and Zr dental implants with a peri-implant-related multispecies model with Streptococcus oralis, Actinomyces naeslundii, Veillonella dispar, and Porphyromonas gingivalis, for 3 and 14 days. Quantitative assessment was performed with the measurement of total bacterial viability (colony forming units, CFU/mg). Scanning electron microscopy (SEM) was used to evaluate biofilm formation on different aspects of the implants. Results. Three-day-old biofilm on Ti implants was significantly higher than t...
Purpose: The aim of the study was to investigate the dynamic process of biofilm adhesion on titanium implant with two surface treatments, either pickled (PT) or moderately roughened by sandblasting with large grits and acid-etched (SLA). Materials and methods: Two types of titanium disks with various surface treatments, i.e. PT and SLA with respective surface roughness (S a ) of 0.3 μm and 1.4 μm, were used as substrata. Three types of biofilms, Streptococcus mutans, Streptococcus sanguinis and polymicrobial biofilms (Microcosm), were grown on the two respective types of titanium disks for 2 h, 1 day, and 7 days. The formation of the biofilms was quantified by colony forming unit (CFU) count, and the structure of the biofilms on the titanium disks was observed by scanning electron microscope (SEM). Results: At 2 h, the number of bacterial cells adhered to SLA surfaces was significantly higher than those to PT surfaces for all tested microorganisms. On day 1 and 7, no differences in biofilm CFU counts were observed between SLA and PT surfaces for S. mutans and Microcosm biofilms, while the S. sanguinis biofilm formation on SLA surfaces was significantly higher than the biofilm on PT surfaces throughout the whole test period. SEM images showed the increasing biofilm formation in time for all types of biofilms. Microcosm biofilms displayed different morphology from the other two single-species biofilms. Conclusions: The higher roughness of a titanium surface would favor the early bacterial adhesion of S. mutans, S. sanguinis and Microcosm. However, as the biofilm became mature, the influence of surface roughness was diminished in a bacterial species dependent manner. Our results underline the importance of dynamic biofilm formation process in the implant study.
The Journal of Prosthetic Dentistry, 2017
The biologic complications of peri-implantitis may present a problem during dental implant therapy. 1-3 Peri-implantitis is associated with about 17% of installed implants after 10 to 16 years of follow-up. 4 In a recent review, Mombelli et al 5 revealed that the prevalence of peri-implantitis seems to be 20% between 5 and 10 years after implantation. Although peri-implant disease is immunologically stimulated by the host, the inflammatory process is bacteria dependent. A similar pattern of colonization is present in periodontal and periimplant disease, 6-10 although with some differences in patients with partial and complete edentulism. 11 This disease pattern has direct clinical implications, especially with regard to choosing implant surface materials. Surface attachment is the first step to biofilm development and is an important precursor to the adaptive behavior of bacteria species as it is the base for the colonization and invasion of pathogens. 12 The hydrophobic attractive forces and electrostatic charge interactions between cells and material surfaces 13-16 are Supported by the São Paulo Research Foundation (FAPESP; grant 2011/05106-6), Coordination for the Improvement of Higher Level Education Personnel (CAPES; grant 5603/13-7), and the Lemann Foundation. Conexão Sistema de Prótese donated the pure commercially available titanium and zirconia stabilized with yttrium disks used in this study.
Frontiers in Dental Medicine, 2021
Implant-related oral diseases such as peri-implantitis and peri-mucositis are largely initiated by bacterial colonization on artificial implant surfaces. Therefore, implant and abutment material characteristics that minimize bacterial attachment and subsequent biofilm formation are important factors in reducing the risk of infection-related implant failure. This study compares the properties of two different titanium-based implant coating materials, titanium nitride (TiN) and titanium carbon nitride (TiCN). Surface hydrophilicity/ hydrophobicity and roughness were evaluated via contact angle measurements and surface profiling with white light interferometry, respectively. TiN-coated surfaces were hydrophobic according to its contact angle higher than 72.7°, whereas TiCN-coated surfaces were hydrophilic with its contact angle of 53.6°. The average roughness (Ra) was greater for TiCN than TiN with the root mean square roughness (Rq) being significantly higher. These findings are in co...