Histamine content and mast cell numbers in tissues of normal and athymic rats (original) (raw)
Tissue histamine levels and mast cell numbers were determined in the skin, tongue and jejunum of female rnu/nu and rnn/+ rats aged between 5 and 29 weeks. The tongue and jejunal mucosa of rnn/nu rats had a larger mast cell density and histamine content than rnu/+. There was a marked increase in subepithelial mast cells in the skin of rnu/nu rats compared with their normal littermates, while mast cell numbers in the deep skin layer and the histamine content were similar in the two groups of rat. Subepithelial skin mast cells were smaller, of more variable shape and contained fewer granules than mast cells in the deep dermal layer, and, unlike the latter, did not emi t a yellow fluorescence after treatment with o-phthalaldehyde. The results indicate that the bulk of the skin histamine is contained in mast cells residing in deep skin layers. They also support the view that the thymus may have a suppressive effect on both mucosai and connective tissue mast cells in vivo.
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Evidence of mast-cell histamine being mitogenic in intact tissue
Agents and Actions, 1985
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Canine cutaneous mast cells dispersion and histamine secretory characterization
Veterinary Immunology and Immunopathology, 1993
In view of the high incidence of canine cutaneous atopic disease and the relevance of mast cells to its pathogenesis, it was considered important to isolate firstly cutaneous mast cells from normal dog skin and to assess the histamine secretory activity, as this can be further used as a tool for the study of canine skin mast cell pharmacology in cutaneous atopy. The procedure for canine dermal mast cell dispersion following a skin enzymatic digestion (as for previous human skin mast cell dispersion methods) is described in detail. The number of canine cutaneous mast cells yielded per gram of skin was 2.31 + 0.21 × 10 ~ representing 1.00% of the total cutaneous cells. The total histamine content per mast cell is 4.93 + 0.39 pg. Net histamine release owing to stimulation by calcium ionophore A23187 (1/zM) and concanavalin A (1 nag ml-~) was respectively 32.17 + 3.56% and 20.39 +_ 2.41% of the total amount per cell. Viability and reactivity to both stimuli of dispersed cutaneous mast cells were similar to the results found in humans. The present study allows further research on the role of mast cells immunopharmacology in allergy by investigation of cells isolated from canine skin in naturally occurring or experimentally induced atopy in the dog to be undertaken.
Agents and Actions, 1989
Like the skin of rats and mice injected with adrenaline (AD), rat isolated peritoneal mast cells display increased levels of perchloric acid-soluble histamine following incubation with AD. Although preexposure to ~-fluoromethyl histidine (FMH), an inhibitor of histidine decarboxylase, prevented the effect of AD in vivo and in vitro, this compound was also found to inhibit mast cell granule swelling evoked by AD, a response linked to histamine changes. Absence of increased levels of isotopic histamine in mast cells incubated with labelled histidine in the presence of AD, as well as the insufficient amounts of would-be precursor histidine found in untreated mast cells, confirm the conclusion that AD does not increase mast cell histamine by stimulating its synthesis.
Mast Cell Degranulation and Histamine Release Observed in a New in Vitro System
Journal of Experimental Medicine, 1960
Mast cells participate in some types of inflammatory reactions involving changes in the microcirculation of certain tissues. Among the known vasoactive substances of importance, histamine has been found in mast cells (1) of several species of animals and in certain species serotonin is also present (2). A variety of substances (the formaldehyde polymer of p-methoxyphenethylmethylamine (48/80), ovomucoid, and dextran) when administered to the rat elicit an inflammatory response, cause histamine and serotonin release and the morphological change of degranulation . No clear description of the process involved in release of the active amines and other mast cell constituents has yet been presented. A major obstacle has been the lack of an appropriate way of observing the action of various agents on the structure of the mast cell and its constituents under controlled conditions. Hence, a new method of observation was sought to study the mechanism of mast cell secretion in vitro.
Local Mitogenic Effect of Tissue Mast Cell Secretion
Cell Proliferation, 1980
The effect of drug-induced mast cell secretion on proliferation .was studied in fibroblast-like and mesothelial-like cells in organ-cultured rat mesentery. Mast cell degranulation achieved by Compound 48/80 was followed by a marked mitogenic reaction in the surrounding tissue cells. The drug itself lacked mitogenic effect on cultured guinea-pig mesentery, the mast cells of which are unresponsive to the drug, and on a human normal fibroblast-like cell line. In contrast, histamine at about lo-'' M, a major mast cell component, induced marked mitogenesis in guinea-pig mesentery without causing degranulation of mast cells.
A Comparative Histological and Histochemical Study of Peritoneal Mast Cells of Albino Rat and Rabbit
Introduction: Mast Cells Is Ubiquitously Present In Connective Tissue Of The Vertebrates And Has Numerous Functions And Plays The Foremost Role In Anaphylactiod Reaction. Aim And Objectives: The Main Purpose Of The Present Work Is To Make A Comparative Histological And Histochemical Study Between Peritoneal Mast Cells Of Albino Rat And Rabbit. Materials and Methods: The Stretch Preparation Of Tissues Were Taken From Albino Rat And Rabbit And Fixed In Two Types Of Fixatives; Formal Alcohol And Neutral Buffered Formalin. Then Subjected To Various Histochemical Stains. Results: Peritoneal Mast Cells Were More Frequent In The Mesentery Of Albino Rat And Less Frequent In Rabbit. Heparin Trisulphate Was Substantial But Heparin Môn sulphate Was Present In Small Amount In Mast Cells Of Albino Rat Whereas Both Types Of Heparin Were Present In Equal Amount In Rabbit. Neutral Mucopolysaccaride Was Found In Small Amount In Mast Cells Of Albino Rat Whereas It Was Absent In Rabbit. Conclusion: Heparin Trisulphate Is An Active Form Of Heparin And Is The Main Constituent Of The Peritoneal Mast Cell Granules Of Albino Rat Whereas In Rabbit Heparin Trisulphate And Heparin Monosulphate Are Present In Equal Amount.
Energy metabolism in rat mast cells in relation to histamine secretion
Pharmacology & Toxicology, 1987
The present survey is based on the following publications Johansen T, Chakravarty N. Dependence of histamine release from rat mast cells on adenosine triphosphate. Naunyn-Schmiedeberg's Arch Pharmacol 1972; 275: 457-63. Johansen T, Chakravarty N. The utilization of adenosine triphosphate in rat mast cells during histamine release induced by anaphylactic reaction and compound 48/80. Naunyn-Schmiedeberg's Arch Pharma-Johansen T. Adenosine triphosphate levels during anaphylactic histamine release in rat mast cells in vitro. Effects of glycolytic and respiratory inhibitors.
2003
The serotonin and histamine content of mast cells and white blood cells in adult male and female rats was compared, using a flow cytometric immunological method. Serotonin was significantly higher in female peritoneal mast cells, peritoneal monocyte-ganulocyte-macrophage cells, blood lymphocytes and blood thymocytes. Histamine was significantly higher in female peritoneal monocyte-granulocyte-macrophage cells, and blood lymphocytes, monocytes and granulocytes, but was significantly less in thymocytes. Peritoneal lymphocytes and the monocyte-granulocyte-macrophage group contained significantly more histamine than mast cells. These experiments call attention to gender differences in the levels of biogenic amines in cells participating in defence reactions, and to the possible non-unique role of mast cells in serotonin and histamine supply.
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