Evaluation of Cross-Reactivity between Holoptelea integrifolia and Parietaria judaica (original) (raw)
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Clinical and Experimental Allergy, 2008
Background The genus Senecio is the largest genus of the family Asteraceae (Compositae).The allergenicity of Senecio has not been assessed previously.Objective The aim of this study was to investigate the allergens of Senecio jacobea pollen and to determine their immunological characteristics and clinical relevance.Methods Fifty patients with rhinoconjunctivitis and a positive skin prick test (SPT) to Senecio were recruited. The clinical relevance of this pollen was assessed by means of a nasal provocation test (NPT). Allergens were characterized by one-dimensional electrophoresis (SDS-PAGE) and two-dimensional gel electrophoresis and immunoblotting. Furthermore, characterization and identification of the allergens were performed by mass spectrometry (MS). In vitro inhibition tests were performed to evaluate cross-reactivity with other pollen.Results Three predominant allergens, both in the intensity of reaction and the frequency of recognition by human-allergic sera, were 59 (60%), 42 (50%) and 31 kDa (50%). The two-dimensional analysis allowed the identification of several allergens. One spot around 42 kDa was identified as a protein homologous to pectate lyase and three other spots were homologous to malate dehydrogenase by MS. S. jacobea proteins showed cross-reactivity with other proteins of the Asteraceae family and also with Parietaria judaica. This was demonstrated by immunoblotting and ELISA inhibition studies.Conclusion S. jacobea constitute a newly discovered allergenic source. It shows cross-reactivity with other members of the Asteraceae plant family as well as with P. judaica.
Comprehensive Study on Key Pollen Allergens
Journal of Pure and Applied Microbiology, 2022
Pollens are typically the primary reason for seasonal hypersensitivity caused in many people that are released by a hundred different species of plants for fertilization. Not all pollens are the same or have the same effect on human beings, there are those worse than others. The human body works out on a defence mechanism by creating certain reactions against those offensive pollens as a response by the immune system. The allergic reactions include sneezing, coughing, wheezing, itching, red-watery swelled eyes, runny nose, inflammation in the nasal passage frequently leading to rhinitis, asthma, skin irritation, and other respiratory disorders. This study is intended to acquire knowledge about a few plants with high allergenic properties along with their major allergens. It is evident that the pollination of the plants varies from season to season as it depends on various factors such as species, weather, and geographical location. Understanding these high allergenic plants with res...
Low molecular weight allergens of the pollen of parietaria officinalis
Molecular Immunology, 1987
The allergenic composition of a low mol. wt fraction of the pollen extract of Parietaria o#kinalis (PO) was investigated. Fraction C, that was eluted after oxytocin (mol. wt 1040) when the pollen extract was gel filtered on Sephadex or on Biogel, was cross-reactive in the RAST with the major allergen PO15 and was capable of eliciting histamine release from leukocytes of sensitive donors. RAST inhibition (RAST I) analysis of the eluate of gel filtration on Sephadex G-10 revealed several peaks of IgE binding activity. Analysis of fine specificity of response of individual patients carried out by skin-prick tests and by RAST I, revealed individual patterns of reactivity, indicating that allergens contained in fraction C were minor allergens.
Purified allergens vs. complete extract in the diagnosis of plane tree pollen allergy
Clinical & Experimental Allergy, 2006
Background Plane tree pollen allergy is a clinical disorder affecting human population in cities of Europe, North America, South Africa, and Australia. Objective To compare IgE-reactivity of the natural and recombinant forms of two major plane allergens, Pla a 1 and Pla a 2, with the reactivity of Platanus acerifolia pollen extract. Methods Forty-seven patients with P. acerifolia allergy, 15 of them monosensitized, and 24 control subjects were included in the study. Natural Pla a 1 and Pla a 2 were purified by standard chromatographic methods and recombinant proteins were expressed in Escherichia coli. Skin prick test and determination of specific IgE were performed with commercial P. acerifolia extract and natural and recombinant purified allergens. Results Pla a 1 and Pla a 2 were responsible for 79% of the IgE-binding capacity against P. acerifolia pollen extract. A high correlation has been found between the IgE response to nPla a 1 (R = 0.80; P o 0.001) or nPla a 2 (R = 0.79; P o 0.001) vs. P. acerifolia extract as well as between natural and recombinant Pla a 1 (R = 0.89; P o 0.001). Skin testing showed no significant differences between extract and nPla a 2, whereas a higher reactivity was found with nPla a 1. In contrast, rPla a 1 revealed markedly reduced sensitivity in comparison with extract by skin prick test and specific IgE. The sensitivity of the mix Pla a 11Pla a 2 was 100% and 87.5% for monosensitized and polysensitized patients, respectively, with no false-positive reactions detected. Conclusion Pla a 1 and Pla 2 are sufficient for a reliable diagnosis of P. acerifolia in most patients and induce comparable skin test reactivity as a whole extract.
Human Immunology, 1996
Par&aria, a plant belonging to the family of Urticaceae, is a major source of allergenic pollen in Europe. In the context of a multinational study, we investigated whether in allergic subjects antibody response towards Par o 1, the major allergen from P. offirinalis, was associated with defined HLA-DRB 1* alleles. The study population consisted of 234 allergic patients: 65 from Bulgaria, 30 from Israel, 99 from Italy, and 40 from Spain. In the Italian study group, the prevalence of ST positivity to Parietaria was 77%. In Parietaria ST-positive subjects, the prevalences of IgG and IgE serum Ab towards Par o 1 were 91% and 7 5%, respectively. HLA-DRBl*llOl and/or 1104 were significantly positively associated with the presence of IgG Ab and with high levels of IgE Ab towards this allergen Cp = 0.0007 and p = 0.012, respectively). ABBREVIATIONS BBS borate-buffered saline CIE crossed immunoelectrophoresis DARIA double antibody radioimmunoassay MHC major histocompatibility complex
Detection of pan-allergens in commercial pollen extracts for allergen immunotherapy
Annals of Allergy, Asthma & Immunology, 2016
Background: Up to 50% of patients with pollen allergy are sensitized to at least 1 of the 2 pollen panallergens profilin and polcalcin. These allergens could have clinical relevance but the content of profilin and polcalcin in commercial extracts for allergen immunotherapy (AIT) is unknown. Objective: To detect these pan-allergens in commercial pollen extracts for AIT from various sources. Methods: Immunoglobulin E (IgE) reactivity to Phl p 7 and Bet v 2 of sera from 18 adults hypersensitive to profilin and/or polcalcin was investigated by enzyme-linked immunosorbent assay before and after absorption with grass, birch, ragweed, pellitory, and olive pollen extracts for AIT from different producers. Immunoblot inhibition experiments also were carried out using the same allergens. Results: Birch, grass, ragweed, and olive pollen extracts for AIT contained large amounts of profilin, inducing 80% to 90% inhibition in most cases; Parietaria AIT extract appeared to contain little profilin. On immunoblot, grass and birch pollen extracts for sublingual AIT completely absorbed IgE specific for rBet v 2. Interestingly, only grass pollen extracts induced a significant inhibition of IgE binding to rPhl p 7 on enzyme-linked immunosorbent assay and immunoblot. A grass pollen allergoid lost most of its inhibitory potency, suggesting a much weakened affinity for specific IgE. Conclusion: With the exception of Parietaria, commercial extracts for AIT of most pollens are rich in profilin and, hence, potentially able to desensitize to this allergen; in contrast, only grass pollen extracts seem rich in polcalcin. These are the pollens to use in case of severe symptoms induced by pollen pan-allergens.
Background: Rapeseed-mustard is the second most important source of edible oil in India. Several species of Brassica is grown in different parts of country for its oilseeds. Objective: The objective was to investigate allergenicity to antigenic extracts of pollen of 4 species of Brassica. Methods: B. campestris, B. juncea, B. nigra, B. napus were selected for the detailed investigation. Pollen samples from each of the four species were collected from the polliniferous materials. The antigenic and allergenic profiles of these extracts were evaluated by means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, Skin prick test, enzyme linked immuno sorbent assay and Western blot on atopic individuals. Results: Out of the 159 atopic subjects tested, 21.38% were positive to at least one or other species of Brassica pollen, with highest skin positivity (13.20%) to B. campestris extract. Raised IgE with significant linear correlation with intensity of skin reactions was obtained....
2004
Objective: Pollen grains of the Euphorbiaceae family are well known causative agents of respiratory allergies in India, European countries and USA. Mallotus phillipensis belongs to the same family and may have some common allergenic properties. It has thus been evaluated for the first time in Indian population for its pollinosis causing properties. Methods: Pollen antigen of Mallotus phillipensis (MP) was extracted and characterized for its protein components by biochemical methods. Pollinosis potency of crude extract of MP pollen was evaluated by skin prick test on population residing in different parts of India. Specific IgE binding characteristics of the extract were determined by ELISA and Immunoblot. Results: Marked skin reactivity in 5.7% atopic population was recorded and subjects constituting 23.8% of the total patients tested showed skin sensitivity to the MP pollen antigen. Significantly raised specific IgE against MP pollen were recorded in 50% of the skin test positive patients. A number of protein bands were detected in a wide Molecular weight range as well as in acidic pI range, by SDS-PAGE and IEF, respectively. A total 11 protein fractions were detected by the specific IgE antibodies on immunoblotting with patient's sera and were considered allergenic. Conclusion: Patients from different geographical regions have shown sensitization to MP pollen antigen. Many proteins have similar molecular weights and pI as other allergenic members of the family (Ricinus communis and Putranjiva roxburghii) found in India, which constitutes a good reason for studying cross reactivity among the members of family Euphorbiaceae, in the future. Key Words: Mallotus phillipensis, allergenicity, atopic patients, Immunoblot, Cross reactivity includes important allergenic plants such as Ricinus communis and Putranjiva roxburghi from India, [13-16] and Merculiaris annua and Haevea brasiliansis from Assessment of allergenicity to Mallotus Phillipensis pollen
Clinical and Experimental Allergy, 2006
SummaryBackground Parietaria pollen is one of the most important causes of pollinosis in Mediterranean countries. Parietaria judaica pollen extract presents two major allergens, Par j 1 and Par j 2, that belong to the lipid transfer protein family.Objective To develop an ELISA for quantification of both major allergens of P. judaica pollen extracts, and to assert correlation of these allergens content with the allergenic activity of extracts.Methods Natural Par j 1–Par j 2 allergens were purified by gel filtration, ion exchange, and affinity chromatography and identified by mass spectrometry. Rabbit antisera were obtained using this protein preparation as antigen and used for immunoaffinity purification of nPar j 1–Par j 2. BALB/c mice were immunized with the immunopurified nPar j 1–Par j 2 and after fusion and screening by direct ELISA, 5D4 monoclonal antibody was selected as capture antibody to develop a quantitative two-site ELISA. Bound proteins were detected by a biotinylated Par j 1–Par j 2-specific polyclonal antibody.Results The optimized ELISA was developed from 25 to 8000 pg/mL of purified Par j 1–Par j 2, and a linear portion of 200–1000 pg/mL. The intraassay and interassay coefficients of variation were lower than 7% and 14% respectively. The assay was very sensitive and specific as it had a detection limit of 25 pg/mL and did not detect reactivity with the same family plants, as Urtica. Par j 1–Par j 2 allergens content was measured in 14 P. judaica and two P. officinalis pollen extracts showing a significant correlation with their allergenic activity measured by enzyme allergosorbent test inhibition.Conclusions The results proved the usefulness of the two-sandwich ELISA for the standardization of Parietaria pollen extracts intended for clinical use, because of its good correlation with allergenic potency.