Phytochemical and CNS activity of Lepidium Sativum Linn. seeds total alkaloid (original) (raw)

Antinociceptive activity of aqueous extract of Lepidium sativum L. in mice

Eastern Journal of Medicine, 2015

Antinociceptive activity of aqueous extract of Lepidium Sativum L. in mice Abstract Purpose: In the present study the aqueous extract of Lepidium Sativum L. (family: Brassicaceae) was investigated for possible antinociceptive effect in Swiss - albino male mice. Material and Methods: In this experiment three groups of male mice were used (n=6). Two models were used to study the effects of the extracts on nociception, acetic acid-induced writhing test and hot plate test in mice. Lepidium Sativum extract was administered in the dose of 20 mg/kg orally 30 minutes prior to pain induction. Results: The aqueous extract showed significant (p < 0.05) analgesic activity evidenced by increase in the reaction time by hot plate method and significant (p < 0.05) reduction in acetic acid - induced writhings in mice with a maximum effect of 27.00 % reduction. These effects were compared with the control and standard drug, diclofenac sodium (50 mg/kg, p.o). Conclusion: The results indicate tha...

Neuropharmacological screening of Leucas linifolia Spreng

Neuropharmacological screening of Leucas linifolia Spreng, 2012

Globally, plant research for search of new therapeutic agent, the treatment of neurological disorder has been progressed constantly, indicating the pharmacological effectiveness of different plant species in a variety of animal models. Therefore, the present study was carried out to evaluate the psychological study for methanolic and ethyl acetate extracts of Leucas linifolia Spreng whole plant. Leucas linifolia Spreng (Lamiaceae) is common herb in India and considered sedative, cynogenetic and stimulant. In the present study, crude methanol (ME) and ethyl acetate (EA) extracts of aerial parts of Leucas linifolia have been evaluated for central nervous system (CNS) activities. Significant central and peripheral nociceptive activity (p < 0.01) was observed for both extracts. Methanolic and ethyl acetate extracts have also showed significant (p < 0.01) decrease in motor activity and fall off time of animals on rotating rod, along with significant (p < 0.01) sedative effect by potentiating phenobarbitone-induced sleeping time. In the acute toxicity study, both extracts were found to be safe up to 2500 mg/kg b.w. These results suggested that methanolic and ethyl acetate extracts of Leucas linifolia show analgesic, anxiolytic and sedative effects. Further investigations are yet, necessary to explore mechanism(s) of action involved in these pharmacological activities.

Pharmacognostical Evaluation of Seeds of Lepidium sativum Linn

International Journal of Pharmaceutical Biological Archive, 2012

Seeds of Lepidium sativum Linn. (Brassicaceae), commonly known as Chandrashoor (seeds) is a highly reputed drug in classical text of Ayurveda mainly indicated in the management of hiccough and diarrhea. It is incorporated in the group of Chaturbeeja (Four seeds) which is indicated in the managements of backache, tympanitis pain and nervous disorders. In spite of its popularity, the drug has not been found to be evaluated well as yet. Hence it was thought essential to evaluate the seeds of Lepidium sativum Linn. systematically. The present paper deals with the detailed macroscopic and microscopic evaluation of the seed, which includes powder study and preliminary chemical evaluation which deals with the determination of physicochemical constants and T.L.C. separations. The microscopical studies revealed certain common characters of the family like beaker shaped cells of hypodermis of testa and epidermal cells with mucilage content. Physicochemical constants support the results of A.P.I.. Methanolic extract of T.L.C showed 5 spots of different colours when sprayed with vanillin-sulphuric acid, indicating the presence of steroidal, phenolic, alcoholic and terpene types of component.

Neuropharmacological Activities of Methanol Extract of Albizia lebbeck (L.) Benth

Bangladesh Pharmaceutical Journal, 2018

Albizia lebbeck (Linn.) Benth. is a deciduous tree which is planted as ornamental and avenue tree almost all over Bangladesh. Leaves of the plant are used in ethnomedicine for the treatment of convulsion and CNS related disorder. This study was aimed to evaluate the neuropharmacological effects of the methanol extract of A. lebbeck leaves in Swiss albino mice. The locomotor effect of crude methanol extract of the plant was investigated by open field and hole cross tests while the anxiolytic activity was determined using elevated plus-maze (EPM) and light/dark box (LDB) tests. Furthermore, the sedative activity of the plant extract was assessed by sodium thiopental-induced sleeping time test. The results demonstrated that the methanol extract significantly (p < 0.001) reduced locomotion of the animals in both hole cross and open field tests in dose-dependent manner at 200-400 mg/kg b.w. In both EPM and LDB tests, the plant extract produced significant anxiolytic effect (p < 0.05) at the doses of 100-400 mg/kg b.w. In addition, it showed significant (p < 0.001) dose-dependent decrease in the onset of sleep and an increase in duration of sleep in sodium thiopental-induced sleeping time test. Preliminary phytochemical analyses of the plant extract revealed the presence of alkaloid, flavonoid, glycoside, saponin, tannin and resin. In acute toxicity test, the leaf extract did not exhibit any adverse effect in mice during 7 days treatment. The results of the present studies suggest that the crude methanol extract of A. lebbeck leaves possesses significant CNS depressant, anxiolytic and sedative properties and rationalize the traditional uses of the plant.

Evaluation of Analgesic Activity of Lepidagathis Cristata Willd Leaf Extracts

International Research Journal of Pharmacy, 2016

Aim of the study is to screen the Lepidagathis cristata Willd, leaf extracts for analgesic activity, because the plant was screened only for immunosuppressive, antipyretic activities only, now in the present study the analgesic activity of leaf extracts were performed. The ethanolic, ethyl acetate, chloroform extracts were prepared and are used for analgesic activity in two dose level that is 200 and 400 mg/kg body weight per oral in two screening methods, one is Hot Plate (n=5), another is Tail Immersion method (n=5), and the leaf extracts are showed significant analgesic activity. The plant extracts did not exhibit any mortality up to the dose level 4000 mg/kg. The methanol, Chloroform and Ethyl acetate extracts of leaf was evaluated for analgesic activity.. The 400mg/kg dose of leaf chloroform extract has highest activity in both the experimental models with 62.5% protection after 30min and 47.3% after 60 min with the significance of p< 0.001 when compared with 0 time interval and after 90 min it was shown 50% of protection and all the extracts has graded dose response.

Effects of a water extract of Lepidium meyenii root in different models of persistent pain in rats

Zeitschrift für Naturforschung C

Lepidium meyenii (Walp.), commonly called maca, is an Andean crop belonging to the Brassicaceae family. Maca hypocotils are habitually consumed as customary food as well as traditional remedies for pathological conditions such as infertility. Moreover, the characterization of maca extracts revealed the presence of compounds that are able to modulate the nervous system. Aimed to evaluate the efficacy of L. meyenii in persistent pain, the present study analyzed the effects of a commercial root extract from maca in different animal models reproducing the most common causes of chronic painful pathologies. A qualitative characterization of this commercial extract by high performance liquid chromatography-mass spectrometry and tandem mass spectrometry analyses allowed us to confirm the presence of some macamides known as bioactive constituents of this root and the absence of the main aromatic glucosinolates. The acute oral administration of maca extract is able to reduce mechanical hypersensitivity and postural unbalance induced by the intra-articular injection of monoiodoacetate and the chronic-constriction injury of the sciatic nerve. Furthermore, L. meyenii extract reverts pain threshold alterations evoked by oxaliplatin and paclitaxel. A good safety profile in mice and rats was shown. In conclusion, the present maca extract could be considered as a therapeutic opportunity to relieve articular and neuropathic pain.

Analgesic Activity of Lepidagathis Cristata Willd Flower Extracts

International journal of Research in Ayurveda & Pharmacy, 2013

Aim of the study was to screen the Lepidagathis cristata Willd, flower extracts for analgesic activity. In the present study the analgesic activity of flower extracts was performed. The methanolic, ethyl acetate, chloroform extracts were prepared and were used for analgesic activity in two dose level of 200 and 400mg/kg body weight in two screening methods, Hot Plate (n=5) and Tail Immersion method (n=5). The flower extracts showed significant analgesic activity. The plant extracts did not exhibit any mortality up to the dose level 4000mg/kg. The methanol, chloroform and ethyl acetate extracts of flower was evaluated for analgesic activity. The flower ethyl acetate extract of Lepidagathis cristata showed 47% and 57.1% activity at 200 and 400mg/kg.b.wt, after 30 min by Eddy's Hot plate Method respectively. The flower chloroform extract showed 43.7% and 44.7% protection at 200, 400mg/kg respectively. The flower methanol, chloroform and ethyl acetate extracts showed dose dependent analgesic activity in thermal models. The flower ethylacetate extract has maximum analgesic activity with 57.1% (p < 0.001 c).

Neuropharmacological Profile of Methanolic Extract of Leucas aspera Leaves in Swiss Albino Mice

2018

Leucas aspera (L. aspera) is commonly known as “Darkolos or Dandokolos” in Bangladesh. The plant is used for the treatments of antipyretic, anti-rheumatic, anti-inflammatory, and anti-bacterial diseases. The purpose of this present study was to investigate the central nervous system (CNS) depressant activities of methanolic extract of L. aspera leaves (MELA) in mice models. The central nervous system (CNS) depressant activity of the MELA was evaluated using open field, whole cross, force swimming, tail suspension, and thiopental sodiuminduced sleeping time tests. For CNS tests, diazepam (1 mg/kg, i.p.) was used as reference drug. In all mice models, MELA was administered orally at the doses of 250, 500, and 750 mg/kg, where as the control group expected deionized water (0.1 mL/mouse, p.o.). The present study indicated that MELA significantly decreased locomotor activity of open field and whole cross tests in mice. The extract significantly increased the duration of immobility time b...

Phytoconstituents, Pharmacological Activity, and Medicinal Use of Lepidium Sativum LINN.: A Review

Asian Journal of Pharmaceutical and Clinical Research, 2019

Herbal medicines are in nice demand within the developed additionally as developing countries for primary aid due to their wide biological and medicative activities, higher safety margins, and lesser prices. Lepidium sativum Linn. (Brassicaceae) is annual herb regionally referred to as halon in India, however, usually referred to as garden cress. L. sativum is a fast growing edible plant. Seeds, roots, and leaves of garden cress have economic importance; however, the crop is especially cultivated for seeds. It is a therapeutic vital herb in India. L. sativum has various pharmacological actions such as antibacterial activity, antifungal activity, antioxidant activity, cytotoxic activity, diuretic activity, hepatoprotective activity, hypoglycemic activity, antiosteoporotic activity, antiasthmatic activity, anti-carcinogenic effect, cardiotonic activity, smooth and skeletal muscles contraction activity, fracture healing property, chemoprotective effects, and hemagglutinating activity. ...

Evaluation of Neuropharmacological Activity of Medicinal Plant

Asian Journal of Pharmaceutical and Clinical Research, 2018

Objective: The objective of the study is to evaluate the antiparkinsonian activity of hydroalcoholic extract of the seeds of Canavalia gladiata (HECG) in zebrafish and Swiss albino mice. Materials and Methods: Catalepsy was induced in zebrafish by exposing them to haloperidol solution. Treatment groups were exposed to bromocriptine and HECG, 30 min before haloperidol exposure at the dose of 2, 5, and 10 μg/mL. Latency to travel from one fixed point to another, time spent near the bottom of the tank, and complete cataleptic time were evaluated to assess behavioral changes. In mice, catalepsy was induced using haloperidol (1 mg/kg i.p.). Treatment groups received bromocriptine (2.5 mg/kg) and HECG at the dose of (100, 200, and 300 mg/kg) orally. Bar test for catalepsy, motor coordination test by rotarod, and locomotor activity by actophotometer were carried out to assess behavioral changes. Results: Bromocriptine and HECG-treated groups showed significant difference in behavioral parameters as compared to haloperidol control group in both the experimental models. Conclusion: Canavalia gladiata seeds exhibited significant antiparkinsonian activity in haloperidol mouse model and zebrafish. Zebrafish can be used with ease and effectiveness for initial screening of drugs before subjecting them to rodent testing.