Antioxidant capacities, phenolic, flavonoid and carotenoid content of various polarities extracts from three organs of Sechium edule (Jacq.) Swartz (original) (raw)

2015, Journal of chemical and pharmaceutical research

Abstract

The objectives of this research were to study antio xidant activities from various fruit extracts of th ree organs of S. edule using two methods of antioxidant assays which were DPPH (2,2-diphenyl-1-picrylhydrazyl) and CUPR AC (Cupric ion Reducing Antioxidant Capacity) and corr elation of their total flavonoid, phenolic and caro tenoid content with IC50 of DPPH antioxidant activities and EC 50 of CUPRAC capacities. Extraction was performed by reflux apparatus using different polarity solvents. The extracts were evaporated using rotary evaporat or. Antioxidant capacities were tested using DPPH and C UPRAC assays. Determination of total phenolic, flav onoid and carotenoid content was performed by spectropho tometer UV-visible and their correlation with IC 50 of DPPH scavenging capacities and EC 50 of CUPRAC capacities were analyzed by Pearson’s me thod. Ethyl acetate pedicel extract of S. edule (PD2) had the lowest IC 50 of DPPH scavenging activity 1.3 μg/ml, while ethyl acetate fruit...

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References (27)

  1. S Patel; T Patel; K Parmer; B Patel; P Patel, J Adv Res in Pharm and Biol, 2011; 1: 120 -129.
  2. F Pourmorad; SJ Hosseinimehr; N Shahabimajd, Afr J Biotechnol, 2006; 5(11): 1142-1145.
  3. K Thaipong; U Boonprakob; K Crosby; LC Zevallos; DH Byrne, J Food Comp Anal, 2006; 19: 669-675.
  4. LT Ling; UD Palanisamy, Review: Potential antioxidants from tropical plants, In Tech, Kuala Lumpur, 1999; 64-72.
  5. T Siciliano; ND Tommasi; I Morelli; A Braca, J. Agric. Food Chem, 2004; 52: 6510-6515.
  6. MG Lindley, Trends in Food Sci Technol, 1998; 9: 336-340.
  7. I Fidrianny; M Harnovi; M Insanu, Asian J Pharm Clin Res, 2014; 7(3): 186-190.
  8. R Apak; K Guclu; B Demirata; M Ozyurek; SE Celik; B Bektasoglu; KI Berker; D Ozyurt, Molecules, 2007; 12: 1496-1547.
  9. IFF Benzi; JJ Strain, Anal Biochem, 1996; 239: 70-76.
  10. L Müller; K Fröhlich; V Böhm, Food Chem, 2011; 129: 139-148
  11. I Fidrianny; H Nurfitri; Sukrasno, J Chem Pharm Res, 2014; 7(4): 1525-1531.
  12. AAL Ordon˜ez AAL; JD Gomez; MA Vattuone; MI lsla, Food Chem, 2006; 97: 452-458.
  13. PY Chao; SY Lin; KH Lin; YF Liu; JI Hsu, CM Yang; JY Lai, Nutrients, 2014; 6: 2115-2130.
  14. I Fidrianny; A Darmawati; Sukrasno, Int J Pharm Pharm Sci, 2014; 6(2): 858-862.
  15. MS Blois, Nature, 1958; 181: 1199-2000.
  16. AA Bedawey, Characteristics of antioxidant isolated from some plants sources, Shibin El-Kom, Cairo, 2010; 1- 11.
  17. CC Chang; MH Yang; HM Wen; JC Chern, J Food Drug Anal, 2002; 10: 178-182.
  18. XC Li; XZ Wang; DF Chen; SZ Chen, J Funct Food Health Dis, 2011; 1: 232-244.
  19. E Souri; G Amin; H Farsan; TM Barazandeh, DARU J Pharm Sci, 2008; 16: 83 -87.
  20. YL Lu; YH Liu; JH Chyuan; KT Cheng; WL Liang; WC Hou, J Bot Stud , 2012; 53: 207 -214.
  21. C Hall; B Zhao, Fruit and Cereal Bioactives: Sources, Chemistry and Applications, CRC Press, Boca Raton, 2011; 23-24.
  22. KE Heim; AR Tagliaferro; DJ Bobilya, J Nutr Biochem, 2002; 13: 572 -584.
  23. CS Foote, Free radicals in biology, 3rd ed., Academic Press, New York, 1976.
  24. S Beutner; B Bloedorn; T Hoffmann; HD Martin, Methods Enzymol, 2000; 319: 226-241.
  25. M Kobayashi M; Y Sakamoto, Biotech Lett, 1999; 21: 265-269.
  26. DJ Charles DJ, Antioxidant properties of spices, herbs and other, John Willey, London, 2013.
  27. D Huang; B Ou; RL Prior, J Agric Food Chem, 2005; 53: 1841 -1856.