The Effects of Vitamins on Micropropagation of Desiree and Mozart Potatoes (Solanum Tuberosum L.) (original) (raw)
Related papers
2018
The present investigation was conducted to develop a protocol for rapid shoot proliferation of potato by MS medium and different concentrations of plant growth regulators. In this study, the node explant was cultured in MS media supplemented with different concentrations of thiamine-HCl (0.1 and 10 mg.l-1), Pyridoxine- HCl, Nicotinic acid (each 0.5 and 50 mg.l-1) and BAP (0.0, 2 and 4 mg.l-1) for shoot proliferation. Maximum number of lateral shoots was observed in MS medium containing 50 mg.l-1 Nicotinic acid, 50 mg.l-1Pyridoxine- HCl, 10 mg.l-1Thiamine-HCl and 4 mg.l-1BAP. Also, maximum percentage of root and callus formation was observed in combination of MS include vitamins without BAP, the present study describes an efficient method for in vitro shoot proliferation of potato cultivars which could be considered for large scale multiplication and propagation of this important vegetable crop.
Effect of Cultivar and Growth Regulator on In vitro Micropropagation of Potato (Solanum tuberosum L)
The present investigation was carried out aiming to develop a technique for rapid in vitro micropropagation of potato (Solanum tuberosum L) plants. Nodal explants prepared from proliferating shoots of established axenic cultures of four potato cultivars viz Diamant, Alpha, Almera and Agria were used. Explants were incubated on agar solidified (0.8% g) Murashige and Skoog's (MS) medium containing 3% sucrose and supplemented with different concentrations of thiadizuron (TDZ) and benzylaminopurine (BA) alone or in combinations with a-naphthalene acetic acid (NAA).Cultivars studied showed wide variation in their response to the plant growth regulators, best results being obtained for the cultivar Almera. Nodal explants responses to BA and TDZ were cultivar-dependent for number of shoot per explant. The highest number (5.4 shoots/explant) of shoots per explant was obtained for Almera explant cultured on MS medium supplemented with 3.0 mg/l TDZ in combination with 0.1 mg/l NAA. Regene...
Micropropagation of Potato Solanum tuberosum L Micropropagation of Potato Solanum tuberosum L
The effect of cytokinins and combination of cytokinins and auxins on in vitro microtuber formation and growth of two potato cultivars of potato (Solanum tuberosum L.) were evaluated. In the present study sprouts and nodal explants of potato, cultivars Agrija and Andrea, were cultured on MS medium, supplemented with different hormonal combinations. For sprouts as an initial explants were used MS + 4 mg/l KIN and MS + 2 mg/l BAP, and for nodal explants were used MS + 4mg/l KIN + 1mg/l IAA and MS + 2 mg/l BAP+1 mg/l NAA. For rapid sprouting clean popato tubers were in vivo treated with 2 ppm GA 3 . Between the two different explants (nodal segment and sprout) nodal cutting showed the better microtuber formation. The cultivar Agrija showed greater ability for in vitro propagation, with 2.14 tubers per shoot and 13.33% microtuber formation.
Micropropagation of Potato Solanum tuberosum L.
2012
The effect of cytokinins and combination of cytokinins and auxins on in vitro microtuber formation and growth of two potato cultivars of potato (Solanum tuberosum L.) were evaluated. In the present study sprouts and nodal explants of potato, cultivars Agrija and Andrea, were cultured on MS medium, supplemented with different hormonal combinations.
2015
One of the goals of the experiment is to standardization of HgCl 2 treatment for explants sterilization. The objectives also include developing a reproducible cost effective protocol for large scale production of Solanum tuberosum of Cardinal variety plantlets from selectively better clones through plant in vitro propagation methods. Selection of growth regulators for proper multiple shoots regeneration, elongation and root induction. To produce genetically uniform plantlets within a short time capable surviving in natural condition raised in in vitro environment. Shoot tip and nodal segment explants from field grown plants were used as experimental materials in this investigation. All explants were cultured on Murashige and Skoog medium supplemented with various plant growth regulators. For surface sterilization of explants, HgCl 2 (0.1%) for 2 minutes was found to be most effective for complete destroying of surface pathogens and getting healthy tissues. Shoot regeneration was observed from both shoot tips and nodal explants for the studied plant. Maximum number of shoot per culture (17) was recorded and it also obtained the highest average length of the shoot (5cm) in Murashige and Skoog medium containing no hormone. On the other hand 6-benzyl amino purine (0.2mg/l) in 3 media showed the highest rate of shoot multiplication (73%) and the highest average length (4cm). In case of Gibberellic acid (0.1mg/l) in Murashige and Skoog media showed its highest rate of shoot regeneration (82%) and the highest average length (4.5cm). From the overall experiment it was observed that shoot tips are more responsive for micro propagation. In root induction Murashige and Skoog medium supplemented with different concentration (0.5, 1, 1.5 and 2mg/l) of indol-3-acetic acid and kinetin. Indol-3acetic acid and kinetin (1.5+1.5 mg/l) showed its lowest rate of root regeneration (40%) and the average length of the root (1.5 cm). On the contrary Murashige and Skoog medium with no hormone showed the rate of root regeneration (96%) and the highest average length of the root (2.5 cm). The supplemented Murashige and Skoog media with no hormone showed the best performance for root regeneration.
In vitro micropropagation and ex vitro rooting of some potato varieties
Ukrainian Journal of Ecology
The article presents the results on selection of optimal concentrations of nutrient media components and nutrient solution at the stages of clonal micropropagation of potato varieties Lyubava, Kemerovochanin, and Tuleevskiy (actual reproduction, rooting in vitro, adaptation to ex vitro conditions). The influence of some components of the nutrient medium (sucrose, agar-agar, growth regulators, namely α-naphthyl acetic acid, β-indolyl acetic acid, and β-indolyl propionic acid) was studied at the stages of reproduction and rooting in order to obtain regenerants of the studied potato varieties. The best development of plants on nutrient medium with addition of 4 g L-1 of agar-agar was revealed. The addition of sucrose in the concentration of 3-5% contributed to the formation of more internodes. The influence of naphthyl acetic acid, β-indolyl oil, and β-indolyl propionic acids in different concentrations on the rhizogenesis of regenerating plants of three potato varieties at the stages ...
Effect of growth regulators on in vitro multiplication of potato (Solanum tuberosum L.) cv. diamant
2017
Shoot tip and nodal segment explants from field grown plants were used as experimental materials in this investigation. All explants were cultured on MS medium supplemented with various plant growth regulators. For surface sterilization of explants, HgCl 2 (0.1%) for 2 minutes was found to be most effective for complete killing of surface pathogens and getting healthy tissues. Shoot regeneration was observed from both shoot tips and nodal explants for the studied plant. Various concentrations of BAP (0.1, 0.2, 0.3mg/l) and GA 3 (0.1, 0.2, 0.3mg/l) were used for shoot multiplication. In case of BAP, the highest length of shoot was recorded 4 cm and the highest percentage of shoot multiplication (73%) was noticed in MS+0.2mg/l BAP. And in case of GA 3, the highest response for shoot multiplication (82%) was noticed in MS+ 0.1 mg/l GA 3 .But among all of the media formulations used in this experiment, the highest response for shoot multiplication (95%) within7-10 days was noticed in hormone free MS media. In case of root regeneration, the highest percentage (96%) of root induction was recorded in MS medium supplemented with no hormone.
Different Plant Growth Regulators on Improvement of Potato (Solanum tuberosum L.)
Journal of the Institute of Science and Technology
The study compared the effects of MS medium containing 0.1 mg L-1 gibberellic acid (GA3) and α-naphthaleneacetic acid (NAA) in combination with 2.0 mg L-1 of kinetin (KIN), 2.0 mg L-1 of benzyl aminopurine (BAP), 1.0 mg L-1 of zeatine riboside (ZR), and 0.5 mg L-1 of jasmonic acid (JA) for the micropropagation development of three potato (Solanum tuberosum L.) cultivars namely Caspar, Granola and Pasinler-92 using binodal stem explants. The results of this research clearly indicated that inclusion of JA among the other plant growth regulators significantly increased shoot regeneration and other characteristics of all potato cultivars used in the study. The minimum days to shoot proliferation on three cultivars ranged 4.0-5.5 d (best result on cv. Pasinler-92) on MS medium containing 0.5 mg L-1 JA. The minimum time to root initiation (11.0 d) was observed on cv. Pasinler-92 on the same medium. The maximum number of axillary shoots (15.25) and nodes (19.0), maximum shoot length (17.25 cm), leaves (19.0) and roots (25.25) were noted on cv. Caspar. The longest roots (18.45 cm) on cv. Pasinler-92, and the maximum fresh weight (404.87 mg) and dry weight (61.85 mg) of plantlets from cv. Granola were also recorded on MS medium fortified with 0.1 mg L-1 GA3+0.1 mg L-1 NAA+0.5 mg L-1 JA.
Journal of New Sciences, 2014
Alaska, Safran and Spunta are the most important varieties of potatoes used by Tunisian farmers. This study was carried out in four steps. First, study on regeneration of tissue culture protocol was studied using buds as an explant for initiation of culture in MS media supplemented with four different concentrations of an auxin : indole butyric acid (IBA). Growth proliferation showed that optimum regeneration rate was obtained with 0,5 mg/l of IBA. The regenerated plants were cultured using nodal cuttings as explants for further multiplication. In vitro tuberization involving a combination benzyladenine (BA) and paclobutrazol (PBZ) gave good tuberization rate. Yet, liquid media containing 5 mg/l of BA was optimal to produce microtubers for all cultivars. Microtubers were transplanted in the soil, cultured in glasshouse to produce minitubers, they produces,-7,2 healthy minitubers/plant. Microtubers cultured in medium containing sucrose (80 g/l) gave best number of minitubers/plant.