Protective Effects of Tridax procumbens Linn. Leaves on Experimentally Induced Gastric Ulcers in Rats (original) (raw)
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Effect of Tridax procumbens Extracts on Ethanol Induced Gastric Ulceration in Wistar Rats
Journal of Medical Sciences(Faisalabad), 2015
The effect of Tridax procumbens extracts on ethanol induced gastric ulceration in Wistar rats was studied. Twelve Wistar rats weighing 150-250 g were used for the study. Gastric ulceration was induced with 0.2 mL kgG 1 b.wt. of the necrotizing agent (80% ethanol-in-0.1N HCl) and were treated as follows: Group 1 (control) was treated with 0.2 mL kgG 1 b.wt. of the vehicle (placebo), groups 2 and 3 were treated with 100 and 250 mg kgG 1 b.wt. of the extracts, respectively and group 4 was treated with 250 mg kgG 1 b.wt. of the standard drug, omeprazole. The experiments lasted for 21 days. Acute toxicity test (Median lethal dose, LD 50), gastric juice pH, gastric acid concentration, gastric ulcer scores, gastric ulcer index, percentage ulcer protection (% ulcer inhibition), activity levels of superoxide dismutase (SOD), catalase and malondialdehyde (MDA) were determined. The results showed that the extracts highly statistically (p<0.05) increased the gastric juice pH ranging from 3.7±0.36 to 4.47±0.15 compared with pH value of 1.46±0.14 in the control. The percentage ulcer protection in the extract treated groups ranged between 49.5-97.6% in a dose dependent manner. Inhibition of gastric ulceration in the extract (97.6%) and omeprazole (98.7%) treated rats was similar at a dose of 250 mg kgG 1. The control did not exhibit ulcer protection. Activities of superoxide dismutase (SOD) were 87.4±2.44 and 91.53±1.53 µmol mgG 1 at doses of 100 and 250 mg kgG 1 of extract, respectively. There was no statistical difference (p>0.05) between SOD of the extracts (91.53±1.43 µmol mgG 1) and omeprazole (92.03±1.44 µmol mgG 1) both at a dose of 250 mg kgG 1. Malondialdehyde (MDA) was 43.6±0.66 and 33.73±1.17 µmol mgG 1 in the extracts and 42.63±2.14 µmol mgG 1 in omeprazole treated rats. The distilled water treated rats had MDA of 66.87±2.14 µmol mgG 1. The results of the study therefore, suggest that Tridax procumbens extracts possess anti-ulcerogenic and antioxidant properties against 80% ethanol-in-0.1N HCl induced gastric ulceration in Wistar rats.
Biomedicine
Introduction and Aim: Triticum aestivum (wheatgrass) is high in nutrient availability and antioxidant enzymes. It also functions as a detoxifier and aids in the rejuvenation of healthy cells. The current study sought to determine the gastroprotective impact of aqueous ethanolic extract of wheatgrass on the ethanol-induced gastric ulcer. Materials and Methods: Wistar rats were given an oral dosage of 100% ethanol (1 ml/200g) to cause gastric ulcers. A reference medicine called omeprazole was given orally at dosages of 20 mg/kg body weight and 200 and 400 mg/kg of wheatgrass extract, respectively. Blood samples were taken an hour after ethanol administration, and the stomachs of deceased rats were then examined biochemically, macroscopically, and microscopically. Results: The gastric ulcer index considerably decreased after taking wheatgrass extract orally, which showed a considerable attenuation of gastric ulcer. The serum level of TNF-? and the activity of the gastric MPO were b...
The aim of the study was to determine antiulcer activity of whole plant of Trigonella foenum-graecum L on the albino wister rats. Dried whole plant of Trigonella foenum-graecum L was powdered and this coarse powder was extracted with 70% ethanol by soxhlet extraction method to yield an ethanol extract of whole plant of Trigonella foenum-graecum L. The extract was subject to preliminary phytochemical analysis and was evaluated for antiulcer activity against various models such as pylorus ligation, Swimming stress induced and Aspirin induced ulcer models. In acute toxicity study, HATFG was found to be safe till 2000mg/kg. So the doses of HATFG at various concentration of 200 and 400mg/kg body weight was administered orally for prevention of ulcer from Pylorus ligation, Swimming stress and Aspirin induced ulcers. Analytical parameters like percentage of ulcer protection was calculated based on ulcer index and gastric juice volume, pH, Total and free acidity of gastric juice. Preliminary phytochemical analysis of HATFG showed the presence of carbohydrates, amino acids, lipids, alkaloids, flavonoids, tannins, saponins, cardiac glycosides, steroids, and terpenoids. The HATFG has shown significant activity at both 200mg/kg and 400mg/kg dose level in a dose dependent manner. Phytochemicals like tannins, flavonoids and saponins may be responsible for anti ulcer activity of HATFG.
J. Med. Plants. …, 2010
Several plants are used in folk medicine to treat gastrointestinal disorders. Gynura procumbens is a medicinal plant commonly used in traditional treatment of many aliments. In this study, G. procumbens ethanolic leaf extract (GPELE) was used to investigate its gastroprotective effect in adult Sprague dawley rats which were divided into six groups. The rats were orally pre-treated with carboxymethyl cellulose (CMC) solution (ulcer control groups), omeprazole 20 mg/kg (reference group), 50, 100, 200 and 400 mg/kg of GPELE in CMC solution (experimental groups), one hour before oral administration of absolute ethanol to generate gastric mucosal injury. After an additional hour, the rats were sacrificed and the ulcer areas of the gastric walls were determined. The ulcer control group exhibited severe mucosal injury, whereas groups pre-treated with GPELE exhibited significant protection of gastric mucosal injury. These findings were also confirmed by histological studies. Acute toxicity study with a higher dose of 5 g/kg did not manifest any toxicological signs in rats. These results suggest that GPELE promotes ulcer protection as ascertained grossly by significant reduction of ulcer area, and histologically by comparatively decreases in ulcer areas, reduction or absence of edema and leucocytes infiltration of submucosal layer compared to ulcer control group.
Gastroprotective effects of flavonoids in plant extracts
Journal of physiology and pharmacology: an official journal of the Polish Physiological Society
The purpose of this paper is to overview the relations between plant-originated substances and their bioactivity measured in terms of antioxidant, cytoprotective and antiulcer activities. In addition, we assessed whether these compounds are capable of affecting the gastric mucosal lesions induced by absolute ethanol applied intragastrically (i.g.). The following plant-originated flavonoid substances were considered; Solon (Sophoradin extract), Amaranth seed extract, grapefruit-seed extract (GSE) and capsaicin (extract of chilly pepper). The area of gastric mucosa lesions and gastric blood flow were measured in rats with ethanol-induced lesions without (control) and with one of the tested substances without and with capsaicin denervation of afferent nerves or administration of L-nitro-arginine (L-NNA), an inhibitor of nitric oxide synthase (NOS). Male Wistar rats, weighing 180-220 g fasted for 24 h before the study where used 100% ethanol was applied i.g. to induce gastric lesions, whose area was determined by planimetry. Gastric blood flow was assessed using electrolytic regional blood flowmeter. All tested plant-originated substances afforded gastroprotection against ethanol-induced damage and this was accompanied by increase in gastric microcirculation, both changes being reversed by pretreatment with neurotoxic dose of capsaicin or by pretreatment with L-NNA. We conclude that plant-originated flavonoid substances are highly gastroprotective probably due to enhancement of the expression of constitutive NOS and release of NO and neuropeptides such as calcitonin gene related peptide (CGRP) released from sensory afferent nerves increasing gastric microcirculation. 1. We demonstrated that plant-originated substances such as Solon -Sophoradin root originated flavonoid, seed extract of Amaranth, extract of grapefruit seeds (GSE) -Citro, and capsaicin present in chilly pepper, all exerted beneficial and dose-dependent reduction in acute gastric lesions induced by corrosive concentration (100%) of ethanol and this reduction was accompanied by dose-dependent rise in gastric blood flow; 2. The mechanism of this protection is closely related to the increase in gastric microcirculation probably caused by stimulation of afferent nerves and release of NO in the mucosa because the protection and gastric hyperemia could be significantly attenuated following the inactivation of sensory afferents by neurotoxic dose of capsaicin and the application of NOS inhibitor, L-NNA;
Fitoterapia, 2011
The standardized EtOAc, MeOH and 70% EtOH extracts of Tridax procumbens aerial parts showed significant inhibition of rat paw edema at a medium dose of 200 mg/kg and the EtOAC extract was the most active. These extracts were standardized by HPLC with the help of chemical markers. Further, the extracts were evaluated for COX-1 and COX-2 inhibitory activity and EtOAc extract exhibited the highest inhibition of COX-1 and COX-2 at 50 μg/mL. Cent aurein, centaureidin and bergenin were isolated as COX-1 and COX-2 inhibitory principles from the EtOAc extract. The extracts also exhibited antioxidant activity against DPPH and ABTS free radicals. The anti-inflammatory activity of T. procumbens aerial parts could be at least in part due to COX-1, COX-2 enzyme inhibition and free radical-scavenging activities which may be attributed to the presence of flavonoids and other polyphenols in the extracts.
Research Trend, 2023
Since the dawn of time, people have employed plants as a major source of biologically active compounds. Due to toxicity of synthetic drugs and ongoing emergence of bacterial resistance, there is a growing urgency for plant based medication. Tridax procumbens L., a small weed of Asteraceae, is widely recognized for its therauptical properties. Investigating phytochemical components (flavonoids and phenolics), antioxidant and antibacterial capability of Tridax procumbens L. were the goals of the current study. Using methanol and water as solvents, the phytochemicals were extracted from the plant. Deploying the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) technique, antioxidant activity was assessed. The broth dilution technique was used to find out antibacterial activity. The methanolic extract showed better antioxidant capability compared to the aqueous extract and it also prevented the growth of gram positive bacteria (Staphylococcus aureus MTCC 87) at a concentration of 0.8 mg/ml. The findings of this study imply that the plant can be employed as a source of natural antioxidant and antibacterial agent.
2010
Hepatoprotective activity of Tridax procumbens L. a medicinal herb commonly used in folklore system for wound healing and also against jaundice, was evaluated against paracetamol (acetaminophen) induced hepatic damage in male albino rats. Paracetamol (2gm/kg body weight) induced hepatic damage was well manifested by significant increase in the activities of Alanine aminotransferase, Aspartate aminotransferase, Alkaline phosphatase in serum and enhanced lipid peroxidation. On the other hand, the activities of Superoxide dismutase and Catalase in liver tissue were lowered. Consequent to paracetamol induced hepatic injury, the Serum Bilirubin level was increased. Paracetamol toxicity, also resulted in, significant reduction in total serum protein and the hepatic glutathione and glycogen contents. The oral administration of varying doses of ethanolic extract of Tridax procumbens L. (100, 200, 300 and 400mg/kg body weight) for the period of 7 days reversed these altered parameters to nor...
International Journal of Engineering Research and, 2020
Tridax procumbens (T. procumbens) Linn. is a medicinal plant found in tropical, subtropical and mild temperate regions around the world being used in Ayurveda treatment for liver disorders, boils, blisters, cuts, wound healing and as an anticoagulant, antifungal, and insect repellent. The plant is known to contain flavonoids, alkaloids, carotenoids, hydroxycinnamates, lignans, benzoic acid derivatives, phytosterols and tannins. The plant is also associated with endophytes to produce secondary metabolies by endophytes possessing antibacterial and antifungal activities. Different parts of this plant and essential oil are associated with ethnopharmacological properties like wound healing, antibacterial, immunomodulation, antiinflammatory, antidiabetic, vasorelaxant, antihyperlipidemic, anticancer, antiplasmodial, anticoagulation and antihepatic. Most of these studies validate the concept of earlier claims that T. procumbens's potential as a medicinal plant. Further studies are required to unravel other pharmacological activities as well as the target-based mechanism of actions. The review also highlights the need for exploring lead molecules from these myriad of compounds that are of vital importance in drug discovery strategies.