Cytotoxic and antioxidant activity of the biogenic bismuth nanoparticles produced by Delftia sp. SFG (original) (raw)
Related papers
Cytotoxicity of biologically synthesised bismuth nanoparticles against HT-29 cell line
IET nanobiotechnology, 2018
This study was purposed to examine the cytotoxicity and functions of biologically synthesised bismuth nanoparticles (Bi NPs) produced by sp. SFG on human colon adenocarcinoma cell line of HT-29. The structural properties of Bi NPs were investigated using transmission electron microscopy, energy dispersive X-ray, and X-ray diffraction techniques. The cytotoxic effects of Bi NPs were analysed using flow cytometry cell apoptosis while western blot analyses were applied to analyse the cleaved caspase-3 expression. Oxidative stress (OS) damage was determined using the measurement of the glutathione (GSH) and malondialdehyde (MDA) levels and antioxidant activity of superoxide dismutase (SOD) and catalase (CAT) levels. The half maximal inhibitory concentration (IC) value of Bi NPs was measured to be 28.7 ± 1.4 µg/ml on HT-29 cell line. The viability of HT-29 represented a concentration-dependent pattern (5-80 µg/ml). The mode of Bi NPs induced apoptosis was found to be mainly related to la...
In vitro cytotoxicity of surface modified bismuth nanoparticles
Journal of Materials Science: Materials in Medicine, 2012
This paper describes in vitro cytotoxicity of bismuth nanoparticles revealed by three complementary assays (MTT, G6PD, and calcein AM/EthD-1). The results show that bismuth nanoparticles are more toxic than most previously reported bismuth compounds. Concentration dependent cytotoxicities have been observed for bismuth nanoparticles and surface modified bismuth nanoparticles. The bismuth nanoparticles are non-toxic at concentration of 0.5 nM. Nanoparticles at high concentration (50 nM) kill 45, 52, 41, 34 % HeLa cells for bare nanoparticles, amine terminated bismuth nanoparticles, silica coated bismuth nanoparticles, and polyethylene glycol (PEG) modified bismuth nanoparticles, respectively; which indicates cytotoxicity in terms of cell viability is in the descending order of amine terminated bismuth nanoparticles, bare bismuth nanoparticles, silica coated bismuth nanoparticles, and PEG modified bismuth nanoparticles. HeLa cells are more susceptible to toxicity from bismuth nanoparticles than MG-63 cells. The simultaneous use of three toxicity assays provides information on how nanoparticles interact with cells. Silica coated bismuth nanoparticles can damage cellular membrane yet keep mitochondria less influenced; while amine terminated bismuth nanoparticles can affect the metabolic functions of cells. The findings have important implications for caution of nanoparticle exposure and evaluating toxicity of bismuth nanoparticles.
Investigation of Bismuth Nanoparticles Antimicrobial Activity against High Pathogen Microorganisms
Spherical bismuth nanoparticles with average size 40 nm have been synthesized by the colloidal-chemical method in water medium. 100% of Bi content has been revealed in the nanoparticles by the method of energy-dispersive X-ray spectroscopy. Synthesized bismuth nanoparticles have been characterized as biosafe and biocompatible according to the Guidelines «Safety assessment of medical nanopreparations». Bismuth nanoparticles' antimicrobial activity in vitro has been estimated against wide spectra of high pathogen microorganisms-potential causative agents of human and animals' diseases. Bismuth nanoparticles' high bactericidal action against all
Spherical bismuth nanoparticles with average size 40 nm have been synthesized by the colloidal-chemical method in water medium. 100% of Bi content has been revealed in the nanoparticles by the method of energy-dispersive X-ray spectroscopy. Synthesized bismuth nanoparticles have been characterized as biosafe and biocompatible according to the Guidelines «Safety assessment of medical nanopreparations». Bismuth nanoparticles' antimicrobial activity in vitro has been estimated against wide spectra of high pathogen microorganisms-potential causative agents of human and animals' diseases. Bismuth nanoparticles' high bactericidal action against all
The use of plant extracts in the synthesis of metal nanoparticles is a very attractive approach in the field of green synthesis. To benefit from the potential synergy between the biological activities of the Moringa oleifera leaves extract and metallic bismuth, our study aimed at synthesizing bismuth nanoparticles using a hydroalcoholic extract of M. oleifera leaves as a means of green synthesis that yields nontoxic products and reduces the production of wasteful material. To this end, the M. oleifera leaves extract was treated with a bismuth nitrate pentahydrate solution. A color change from light brown to dark brown indicates the synthesis of bismuth nanoparticles. The total phenolic content in the M. oleifera leaves extract used was 23.0 ± 0.3 mg gallic acid equivalent/g of dried M. oleifera leaves powder. Antioxidant property of MO synthesised bismuth Nanoparticles was evaluated and in line with the extract used in the synthesis of NPs. The physical properties of the synthesized...
Cytotoxicity of bismuth nanoparticles in the murine macrophage cell line RAW 264.7
Journal of Materials Science: Materials in Medicine, 2020
A promising use of bismuth nanoparticles (BiNPs) for different biomedical applications leads to a search for the elucidation of their toxicity mechanisms, since toxicity studies are still at early stage. In the current study, cytotoxic effects of BiNPs produced by laser ablation in solution (LASiS) was investigated in the murine macrophage line RAW 264.7. The cells were exposed to 0.01-50 µg ml −1 of BiNPs for 24 and 48 h and then cytotoxicity assays were performed. Decrease of MTT conversion to formazan and of cell attachment were observed with no effects on cell proliferation. No loss of membrane integrity or significant changes of ROS and RNS levels were observed in exposed cells. Foremost, increased phagocytic activity and DNA repair foci occurred for cells exposed to BiNPs. These effects are important findings that must be considered in the case of biomedical application of BiNPs, since inappropriate macrophages activation and inactivation may lead to immunotoxicity.
Advanced Pharmaceutical Bulletin
Purpose: Lately, bismuth-based nanomaterials have been widely utilized in medical researches such as imaging, drug delivery and radio-sensitization. Despite their advantages, bismuth-based compounds have shown toxic effects in humans. There are few studies on cytotoxicity effects of bismuth oxide (Bi2O3) nanoparticles (NPs) in-vitro. In this study, we aimed to investigate cytotoxicity of bare and also folate and 5-aminolevulinic acid (5-ALA)-conjugated Bi2O3 NPs on nasopharyngeal carcinoma (KB) and lung cancer (A549) cell lines. Methods: Bi2O3 NPs were synthesized and conjugated with folate and 5-ALA. KB and A549 cells were cultured and incubated with 10, 20, 50 and 100 μg/ml concentrations of bare and folate-5-ALA-conjugated NPs. The survival rates were obtained after 2 and 24 hours incubation of the cells with NPs using MTT assay. Also, apoptosis and ROS generation induced by the NPs in the treated cells were obtained using Caspases-3 activity assay and flow cytometry analysis, re...
Toxicology in vitro : an international journal published in association with BIBRA, 2018
Nanoparticles (NPs) have emerged as new potential tools for many applications in previous years. Among all types of NPs, bismuth NPs (BiNPs) have a very low cost and potential for many applications, ranging from medicine to industry. Although the toxic effects of bismuth have been studied, little is known about its toxicity at the nanoscale level. Therefore, in this study, we aimed to investigate the cytotoxic effects of BiNPs produced by laser ablation synthesis in solution (LASiS) in a reference mammalian cell line to evaluate their cytotoxicity (BALB/c 3 T3 cells). We also stabilized BiNPs in two different solutions: culture medium supplemented with fetal bovine serum (FBS) and bovine serum albumin (BSA). The cytotoxicity of BiNPs in culture medium (IC:28.51 ± 9.96 μg/ml) and in BSA (IC:25.54 ± 8.37 μg/ml) was assessed, and they were not significantly different. Second, the LD was predicted, and BiNPs were estimated as GHS class 4. We also found that cell death occurs due to apop...
Research Square (Research Square), 2024
This study explored the synthesis and characterization of iron nanoparticles (NPs) using Micromeria bi ora extract. The rapid reduction of iron ions, evidenced by a distinct color change, signi es an e cient interaction, leading to successful NPs formation. UV-visible spectroscopy con rmed the synthesis, revealing an absorption peak at 295 nm that intensi ed over time. Fourier Transform Infrared (FTIR) spectroscopy demonstrates phytochemical involvement. Field-Emission Scanning Electron Microscopy (FESEM) images displayed cuboctahedron-shaped NPs with various facet formations, which are crucial for diverse applications. DISCUS package was used to simulate the shape and decorate the surface with organic molecules obtained from the extract. Energy Dispersive X-ray Spectroscopy (EDS) was used to con rm the elemental composition. Additionally, potential applications, including enzyme effects and sedative and anti-in ammatory properties, were explored. The extract and NP showed anticancer effects against MDR2780AD cell lines, with IC 50 values of 1.99 and 0.91 respectively. The tested Iron NPs showed 92.22, 76.22, and 88.23% inhibitory effects against urease, CA-II, and XO, respectively. The maximum percentage analgesic effects of the extract (100 mg/kg) and NPs (10 mg/kg) were 65 and 82, respectively. Maximum anti-in ammatory effect was observed at the third hour of treatment. The antiin ammatory effect of NP (90%) was superior to that of the extract (60%).