Biosynthesis of silver nanoparticles from biowaste pomegranate peels (original) (raw)
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The physical and chemical method employed in production of silver nanoparticles is expensive and the reagents used are toxic. This paper deals with the production of silver nanoparticles using biological compounds by biosynthesis method. Silver nitrate is reduced by the peel extracts of Pomegranate, which act as a reducing agent as well as stabilizing agent due to the presence of biomolecules. The characterization of the silver nanoparticles was done using XRD and FTIR. The approach of fruit-mediated synthesis appears to be cost efficient eco-friendly and easy alternative to conventional methods of silver nanoparticles synthesis.
Synthesis of Pomegranate Peel Extract Mediated Silver Nanoparticles and its Antibacterial Activity
American Journal of Advanced Drug Delivery, 2014
In this report a simple and eco-friendly biosynthesis of silver nanoparticles using Pomegranate peel extract as the reducing agent from 1 mM AgNO3 had been investigated. The formation of silver nanoparticles was characterized by UV-Vis spectrum, Fourier Transform Infrared Spectroscopy (FT-IR) and Scanning Electron Microscopic (SEM) analysis. The UV-Vis spectra results show a strong resonance centered on the surface of silver nanoparticles (AgNPs) at 371 nm. The Fourier Transformation Infrared Spectroscopy spectral study demonstrates pomegranate peel extract acted as the reducing agent. The scanning electron microscopic (SEM) analysis shows nanoparticles with the average particles size ranges about 5-50 nm. Further the antibacterial activity of AgNPs was evaluated against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli pathogens. This route is rapid, simple, without any hazardous chemicals as reducing or stabilizing agents and economical to synthesize AgNPs.
Biosynthesis of Silver Nano Particles through Epicarp of Pomegranate Fruit
The application of “green synthesis” rules to nanomedicine and nanotechnology is very important for the preparation of nanoparticles. In this work we successfully synthesized an ecofriendly, one step method for preparing silver nanoparticles (AgNPs) in natural condition. The silver nanoparticles were synthesized in aqueous solution, using silver nitrate mixed with epicarp of pomegranate fruit (which act as a reducing and capping agent). The size of synthesized AgNPs was studied at different reaction time. The UV-Vis spectra were in excellent agreement with obtained nanostructure studied performed by (TEM) for their size distribution. Further these biologically synthesized nanoparticles were found to be highly toxic against pathogens.
Phytofabrication of silver nanoparticles using pomegranate fruit seeds
The cost effective and ecofriendly technique of green synthesis of silver nanoparticles from the extract of seed of pomegranate fruit was demonstrated. The reduction process was simple and convenient to handle and was monitored by UV-Vis spectroscopy. The morphology and crystalline phase of synthesized nanoparticles were determined from transmission electron microscopy (TEM) and X-ray diffraction (XRD) method. These biologically synthesized nanoparticles were found to highly toxic against different multi-drug resistant human pathogens. Therefore, these particles can be exploited for curing of infection and other nanotechnology based therapeutic industries.
Biosynthesis of silver nanoparticles using plant extracts: an update
2019
In the recent period, different types of nanoparticles (NPs) have been proposed to improve antioxidant, but also antimicrobial properties of various natural compounds. Much attention has been dedicated to synthesis of NPs using biogenic enzymatic processes. The biosynthesis of NPs has been claimed to be superior to chemical synthesis, especially because of the opportunity of producing more environment-friendly and less toxic products. Among the numerous types of NPs, bioreduction-produced silver NPs from ionic silver-containing solutions are receiving much attention. In this work, we present an update on our investigation on biosynthesis of silver NPs (AgNPs), thus presenting a method of reduction of silver nitrate solution, using a plant decoct from black pepper fruit (Piper nigrum, L). Namely, we present biosynthesis of AgNPs from 1 mmol/L AgNO3 solution, by bioreduction that was provided from the complex composition of pepper fruit extract, obtained by decoction. The formation of...
Communications in Science and Technology
In this study, biosynthesis of silver nanoparticles using the extract of a local banana peel (variant name: Raja) as bio-reductor was carried out. This study aimed to determine the effect of two different ratios of Banana Peel Extract (BPE)/distilled water on the synthesis of silver nanoparticles. The two of BPE/water ratios were 1% (v/v) and 5%(v/v), named as Sample A and Sample B, respectively. Whereas, the concentrations of AgNO3 solution as the precursor were varied as follows: 0.125; 0.1; 0.075; and 0.05 M. The synthesized colloidal silver nanoparticles were characterized using a UV-Vis spectrometer, while the BPE solution was analyzed using Fourier Transform Infra-Red (FT-IR) to study its functional groups. While, the solid silver nanoparticles was characterized using a Scanning Electron Microscopy (SEM) with an Energy-dispersive X-ray spectroscopy (EDX) analysis. The UV-Vis spectrometer results qualitatively showed that sample A produced better silver nanoparticles than that ...
International Journal of Nanoparticles, 2017
Silver nanoparticles were successfully synthesised at room temperature from silver nitrate using unripe Carica papaya fruit extract as a reducing and capping agent. PH of the reaction solution was adjusted with the addition of sodium hydroxide from pH 5.6 (original pH of the broth) to pH 6.5, pH 7.5 and pH 8.5. Quasi spherical silver nanoparticles were obtained across the studied pH range. The average particle size obtained decreased from 34 nm to 15 nm as the pH of the solution increased from 5.6 to 8.5. From the observations and ultra violet-visible spectrophotometer analysis, the rate of reduction and formation of silver nanoparticles increased as the pH increased. The crystalline nature of the as-synthesised silver nanoparticles in a cubic structure was confirmed by the peaks in X-ray diffraction and selected area electron diffraction. From Fourier transform infrared spectra, it was found that proteins in unripe papaya fruit extract were strongly bounded to the surface of silver nanoparticles as a capping agent. The thin layer of protein coating also further confirmed under high resolution transmission electron microscope. A plausible formation mechanism has been briefly proposed.
Journal of Manmohan Memorial Institute of Health Sciences
Background: Nanoparticles are those whose size ranges 1 nm to 100 nm. They are in different sizes and shape, such as triangular, spherical, irregular, etc. In recent years, nanoparticles synthesis has received considerable attention due to their unique properties and potential applications. Powdered sample (100gm)was extracted with 800ml ethanol by Soxhlet extraction method for 6 hours. The resultant extract was used to prepare nanoparticles.Methods: The main objective of the present work was to prepare pomegranate and orange peel extract based nano particles by chemical complexation method. Ethanolic extracts of pomegranate and orange peel were prepared by using Soxhlet apparatus and evaluated for phyto-chemical constituents.Results: Qualitative analysis of pomegranate peel showed positive results for Alkaloids, Anthraquinones, Saponins and Terpenoids, where as orange peel showed positive results for Alkaloids, Tannin and Saponins. The percentage moisture content obtained from the ...
Biosynthesis and Characterization of Silver Nanoparticles
Research Square (Research Square), 2022
In this study, a quick, simple, cost-e cient, and green procedure for silver nanoparticles (AgNPs) biosynthesis was executed at 25°C using 5 easily accessible plants from Cameroon including Carica papaya, Achillea millefolium, Perilla frutescens, Ocimum gratissimum, and Garcinia kola. These plants served as capping and reducing agents, while the AgNO 3 salt was the precursor. Initially, bioreduction of metallic Ag + to Ag 0 nanoparticles was established via a reduction in pH. Biosynthesis of AgNPs was primarily a rmed visually via a color change of the reaction mixtures with the ultraviolet-visible (UV-Vis) spectroscopy absorption peaks demonstrating that the synthesized particles were indeed AgNPs. X-ray diffractometry (XRD) showed the nanoparticles were crystalline in nature and had negative zeta potential (ζ-potential) values, which indicate they could be naturally stable. The phytochemical and Fourier transform infrared (FTIR) spectroscopic analyses revealed the possible phytochemicals in each aqueous plant extract responsible for reducing the Ag + metallic ions to nanoparticles followed by capping and stabilization of the nanoparticles. The high-resolution transmission electrons microscopy (HRTEM) micrographs revealed nanoparticles of varying shapes and sizes. Also, micrographs from the scanning electron microscopy (SEM) showed clouds of polydispersed nanoparticles, which were con rmed by energy dispersive X-ray (EDX) spectroscopy to be highly composed of Ag, with strong optical peaks around 3 kV. The results thus validate that these AgNPs can be e ciently formulated using easily available tropical plants for safe applications in various sectors such as medicine and agriculture.
The green synthesis of silver nanoparticles using plant extract has more advantages because they are eco - friendly, easily availability and cost efficient. The bio synthesis of silver nanoparticles using pomegranate seed, peel and leaf its act as a reducing agent as well as capping agent from 1mM AgNO3 has been investigated. The formation of silver nanoparticles was characterized by UV - vis spectrum. The morphology was characterized by scanning electron microscopy (SEM), energy dispersive X - ray analysis (EDAX) and X - ray diffraction (XRD), with the active function al groups present in the synthesized silver nanoparticles being confirmed by Fourier Transform Infra - red (FTIR) spectroscopy. Moreover, their antibacterial activity was evaluated against Pseudomonas, Bacillus cereus, staphylococcus albus and proteus pathog ens. And anti - fungal activity was evaluated against Candida Tropical and Aspergillus. The applications of silver nanoparticles have the medical application like wound dressings, surgical instruments