Antibacterial activity and phytochemical screening of some medicinal plant extracts against bacteria isolated from food materials sold in Keffi, Nasarawa State, Nigeria (original) (raw)
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Medical Journal of Babylon, 2008
Antibacterial activity of some local plant extracts: Cumin (Cuminum cyminum), harmal (Peganum harmala) and pomegranate (Punica granatum)were determined against some bacterial species(Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Salmonella typhi and Proteus mirabilis). These extracts showed variation in their inhibitory effects, the pomegranate(Punica granatum)extract revealed the highest inhibitory activity against most bacterial isolates. The minimal inhibitory concentration(MIC)was also varied with the type of plant extract and bacteria. The MIC of the cumin(Cuminum cyminum)was 2.5 gm/dl for E.coli, K.pneumonia and S.typhi. The MIC of harmal(Peganum harmala)was 5gm/dl for K.pneumonia, while the MIC of pomegranate (Punica granatum) was 2.5 gm/dl for E.coli and 5gm/dl for the other isolates.
Antibacterial activity of some plant extracts. IUFS Journal of Biology
2008
Ten aqueous and one ethanolic extracts from medicinal plants used in Turkey were evaluated for antimicrobial activity. Plant extracts were prepared using distilled water and 50 % ethanol. It was found that three plant extracts from the 9 plants studied had antibacterial activity. These activities were produced by the aqueous extracts of Pistacia, Tilia argentea and Anthemis pungens. All of these plant extracts had antibacterial activity against E.coli. Also, Tilia argentea and Pistacia spp inhibited the growth of Bacillus subtilis. In addition to these bacteria, Klebsiella pneumoniae, Staphylococcus aureus and environmental Aeromonas spp. strains were inhibited by Tilia argentea.
Evaluation of antibacterial activity of certain plant extracts against some bacterial strains
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In the present study, methanolic and ethanolic extracts of three plant species namely Lantana camara L., Aegle marmelos (L.) Corr. and Emblica officinalis L. were evaluated for their antibacterial activity against four bacterial strains namely Bacillus subtilis Cohn, Bacillus cerus Farkland and Farkland, Micrococcus luteus (Schr.) Cohn and Escherichia coli (Mig.) Castellani and Chalmers. Two concentrations of extracts viz. 5 and 10% of all plants were used to check their antibacterial activity. Both ethanol and methanol extracts showed considerable antimicrobial potential against given microbes however, ethanol extract was found more effective than the methanol extract. E. officinalis had more antimicrobial activity than the other two plants extracts. Both Ethanolic and methanolic extracts of E. officinalis exhibited increased antibacterial activity around 1.8 folds ( B. cerus ), 1.5-2.0 folds ( M. luteus ) and 1.8-2.0 folds ( E. coli ) as compared to control . In case of B. subtili...
Antibacterial activity of some medicinal plant extracts
Journal of Natural Medicines, 2008
Antibacterial activity of hot aqueous and methanolic extracts prepared from six plants (Terminallia chebula, Terminallia bellerica, Phyllanthus emblica, Punica granatum, Lawsonia alba and Mikania micrantha) used in traditional folk medicines of India were screened against five pathogenic bacteria (Staphylococcus aureus MTCC 2940, Bacillus subtilis MTCC 441, Escherichia coli MTCC 739, Proteus vulgaris MTCC 426 and Enterobacter aerogenes MTCC 111). The highest antibacterial potentiality was exhibited by the methanolic leaf extract of T. chebula, followed by the aqueous fruit extract of T. bellerica. The leaf extract of T. chebula can be considered to be as equally potent as the most effective antibiotics, such as ciprofloxacin, gentamycin, kanamycin, ofloxacin and cephalexin. A sensitivity test performed with commonly used sensitivity test disks resulted in the appearance of multiple drug resistance phenotypes of the bacteria tested. A comparision of data in the inhibition zones of pathogenic bacteria showed that gentamycin, ofloxacin, kanamycin and tobramycin were effective against all of the bacterial strains tested.
Antibacterial activity of some plant extracts
Ten aqueous and one ethanolic extracts from medicinal plants used in Turkey were evaluated for antimicrobial activity. Plant extracts were prepared using distilled water and 50 % ethanol. It was found that three plant extracts from the 9 plants studied had antibacterial activity. These activities were produced by the aqueous extracts of Pistacia, Tilia argentea and Anthemis pungens. All of these plant extracts had antibacterial activity against E.coli. Also, Tilia argentea and Pistacia spp inhibited the growth of Bacillus subtilis. In addition to these bacteria, Klebsiella pneumoniae, Staphylococcus aureus and environmental Aeromonas spp. strains were inhibited by Tilia argentea.
Since the beginning of human civilization, medicinal plants have been used by mankind for its therapeutic value and even in modern times have formed the basis of many pharmaceuticals in use. The aim of the present study was to evaluate the antimicrobial activity of different extracts of Piper betle, Boerhavia diffusa, Oxalis corniculata, Centella asiatica, Camellia sinensis, Curcuma longa and Allium cepa traditionally used in Bangladesh. The antimicrobial activity of these different solvent extracts were tested against Gram-positive (Staphylococcus aureus) and Gram-negative bacterial species (Escherichia coli and Salmonella spp.) by observing the zone of inhibition by disc diffusion method. The tested bacterial species were isolated from hospital waste water sample and fresh poultry meat samples where Chloramphenicol was used as standard for antibacterial assay. The results of the study revealed that the ethanolic, methanolic, and aqueous extracts of almost all the seven herbal species successfully showed to be effective against the tested Gram-positive and Gram-negative bacteria with only negative result observed in case of Centella asiatica. The methanolic and water extract of Boerhavia diffusa was found to be slightly effective producing a shorter zone of inhibition. Among these medicinal plant products, Camellia sinensis and Piper betle was found to be more effective against the tested bacterial species where methanolic extracts showed maximum zone of inhibition against Gram-positive (S. aureus with zone of inhibition of 12 mm and 11 mm respectively) and Gram-negative bacteria (e.g. Salmonella spp. with zone of inhibition of 16 mm and 14 mm). The result confirmed the presence of antibacterial activity of Piper betle extract and other five medicinal plant extracts against various human pathogenic bacteria which should be further tested for other pathogenic multi drug resistant bacteria and could be used as an effective biocontrol agent in future.
Preliminary screening of antimicrobial properties of few medicinal plants
The Journal of Phytology, 2010
Crude extracts were prepared from the leaves of ten medicinal plants viz., Alpinia galanga, Artabotrys uncinatus, Commelina benghalensis, Costus igneus, Euphorbia cyathopora, Justicia gendarussa, Kalanchoe pinnata, Panicum antidotale, Sauropus androgynous and Hibiscus using methanol as solvent and screened for their antibacterial activity against ten bacterial pathogens. The tested gram positive bacterial strains were Bacillus cerus, Bacillus megaterium, Micrococcus leuteus, Staphylococcus aureus, Streptococcus lactis , and gram negative strains were Pseudomonas aeruginosa, Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae and Salmonella typhimurium . Among the ten plants tested, the methanol extracts of Alpinia galanga , Artabotrys uncinatus, Costus igneus and Yellow Hibiscus exhibited higher antibacterial activity when compared to the other plant extracts. These four plant extracts were further used for the phytochemical analysis. Results of the phytochemical analysi...
Idendification test of phytochemical and antibacterial activity of using some medicinal plants
Journal of Pharmacognosy and Phytochemistry, 2020
Objective: To identify phytochemical screening and Antibacterial activity against Staphylococcus aureus and E. coli, Oral bacteria individually. To prepare the agar media plates for testing of different types of bacteria. Methods: Different types of plants are used for containing aqueous extract of Neem (Azadirachta indica), Clove (Syzygium aromaticum), Betel (Piper betle), Turmeric (Curcuma longa), Peppermint (Mentha piperita), and Guava (Psidium guajava) was used for phytochemical screening and antibacterial activity against S.aureus, B. subtilus and E. coli bacteria and identification test of inhibition zone using with different concentration of extract were used (100 mg/ml).Results: Antibacterial activity of Neem (Azadirachta indica), Clove (Syzygium aromaticum), Betel (Piper betle), Turmeric (Curcuma longa), Peppermint (Mentha piperita), and Guava (Psidium guajava) are used as an aqueous extraction of Crude drugs against S.aureus, E.coli and Subtilus. Conclusion: These result s...
Evaluation of Antibacterial Activities of Medicinal Plants
Nepal Journal of Science and Technology, 2013
Plant and plant products are used as medicine from the beginning of human civilization. This study compares the antibacterial activity of crude hexane, ethylacetate and methanol extracts of nine different medicinal plants used in traditional Nepalese medicine, tested against 10 species of bacteria: Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 20063), Klebsiella oxytoca, Proteus mirabilis (ATCC 49132), Proteus vulgaris, Pseudomonas aeruginosa (ATCC 27853), Salmonella typhi, Salmonella paratyphi and Shigella dysenteriae by agar well diffusion method. The selected parts of these medicinal plants namely Acorus calamus (Rhizome), Aegle marmelos (Fruit), Asparagus racemosus (Tuberous root), Mimosa pudica (Root), Terminalia bellirica (Fruit), Terminalia chebula (Fruit), Tinospora cordifolia (Stem), Woodfordia fruticosa (Flower) and Holarrhena antidysenterica (Seed) were taken for study.The result showed that out of nine tested plants, four plant extracts (44%) showed activity against at least five or more tested bacteria and five plant extracts (56%) were active against three or less than three bacteria. None of the tested plant extracts was active against all the tested bacteria. A. racemosus was the least effective against bacterial species. S. aureus was the most susceptible bacteria being sensitive to 18 extracts from 9 medicinal plants. P. vulgaris was the most resistant bacteria being resistant to all selective plants. The MBC value ranges from 3.12 mg/ml to >50 mg/ml. Lowest MBC was shown by ethylacetate extract of T. bellirica against E. coli and ethylacetate extract of W. fruticosa against S. dysenteriae. Largest ZOI (31 mm) was produced by ethylacetate extract of T. bellirica.
Antimicrobial and phytochemical analysis of some indigenous plants
World Researchers Associations, 2019
The antimicrobial activity of the extracts of the Calotropis procera, Datura metel, Cuscuta reflexa, Pongamia pinnata and Nerium oleander has been studied against the pathogenic bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aureuginosa, Klebsiella pneumoiniae, Paenibacillus and Bacilus subtilis. Significant antibacterial activity was observed with extract of ethanol, methanol and chloroform. The ethanol extract of D. metel showed maximum antibacterial activity against the all tested bacteria except the P. aureuginosa. Aqueous extract showed no measurable antibacterial activity. Inhibition was seen as concentration dependent phenomenon. Phytochemical screening revealed the presence of alkaloids, flavanoids, glycosides, steroids, tannins, phenol and saponin.