Subtyping of Cryptosporidium parvum Obtained from Humans and Calves in Van, Turkey (original) (raw)

Iranian Journal of Parasitology

Abstract

Background: We aimed to investigate the prevalence of Cryptosporidium species detected in humans and calves in the Van region of Turkey. Methods: A total of 150 patients, comprising 60 who were immunosuppressed, 50 who were immunosuppressed and had diarrhea, and 40 who had only diarrhea, were enrolled in this study in the Department of Medical Parasitology, Van Yuzuncu Yıl University Faculty of Medicine, Turkey. Stool samples were taken from the rectums of a total of 50 calves that had 30 diarrhea and 20 that did not have diarrhea, from the stables and farms of 10 central villages of Van, Turkey. All samples were analyzed using modified acid-fast staining, immunochromatographic test, and PCR. Cryptosporidium positive samples were also subtyped. Results: Only C. parvum subtypes were detected in all positive samples. C. parvum was detected in 30 (20%) of the 150 human stool samples, while it was detected in 5 (10%) of the 50 samples from the calves. The GP60 gene region was amplified ...

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