Proteome Dynamics of Persulfidation in Leaf Tissue under Light/Dark Conditions and Carbon Deprivation (original) (raw)
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Journal of Experimental Botany, 2017
Hydrogen sulfide-mediated signaling pathways regulate many physiological and pathophysiological processes in mammalian and plant systems. The molecular mechanism by which hydrogen sulfide exerts its action involves the post-translational modification of cysteine residues to form a persulfidated thiol motif, a process called protein persulfidation. We have developed a comparative and quantitative proteomic analysis approach for the detection of endogenous persulfidated proteins in wild-type Arabidopsis and L-CYSTEINE DESULFHYDRASE 1 mutant leaves using the tag-switch method. The 2015 identified persulfidated proteins were isolated from plants grown under controlled conditions, and therefore, at least 5% of the entire Arabidopsis proteome may undergo persulfidation under baseline conditions. Bioinformatic analysis revealed that persulfidated cysteines participate in a wide range of biological functions, regulating important processes such as carbon metabolism, plant responses to abiotic and biotic stresses, plant growth and development, and RNA translation. Quantitative analysis in both genetic backgrounds reveals that protein persulfidation is mainly involved in primary metabolic pathways such as the tricarboxylic acid cycle, glycolysis, and the Calvin cycle, suggesting that this protein modification is a new regulatory component in these pathways.
The Modus Operandi of Hydrogen Sulfide(H2S)-Dependent Protein Persulfidation in Higher Plants
Antioxidants
Protein persulfidation is a post-translational modification (PTM) mediated by hydrogen sulfide (H2S), which affects the thiol group of cysteine residues from target proteins and can have a positive, negative or zero impact on protein function. Due to advances in proteomic techniques, the number of potential protein targets identified in higher plants, which are affected by this PTM, has increased considerably. However, its precise impact on biological function needs to be evaluated at the experimental level in purified proteins in order to identify the specific cysteine(s) residue(s) affected. It also needs to be evaluated at the cellular redox level given the potential interactions among different oxidative post-translational modifications (oxiPTMs), such as S-nitrosation, glutathionylation, sulfenylation, S-cyanylation and S-acylation, which also affect thiol groups. This review aims to provide an updated and comprehensive overview of the important physiological role exerted by pe...
Hydrogen Sulfide Signaling in Plants: Emerging Roles of Protein Persulfidation
Frontiers in plant science, 2018
Hydrogen sulfide (HS) has been largely referred as a toxic gas and environmental hazard, but recent years, it has emerged as an important gas-signaling molecule with effects on multiple physiological processes in both animal and plant systems. The regulatory functions of HS in plants are involved in important processes such as the modulation of defense responses, plant growth and development, and the regulation of senescence and maturation. The main signaling pathway involving sulfide has been proven to be through protein persulfidation (alternatively called sulfhydration), in which the thiol group of cysteine (-SH) in proteins is modified into a persulfide group (-SSH). This modification may cause functional changes in protein activities, structures, and subcellular localizations of the target proteins. New shotgun proteomic approaches and bioinformatic analyses have revealed that persulfidated cysteines regulate important biological processes, highlighting their importance in cell...
Hydrogen sulfide signaling in plant adaptations to adverse conditions: molecular mechanisms
Journal of Experimental Botany, 2021
Hydrogen sulfide (H2S) is a signaling molecule that regulates critical processes and allows plants to adapt to adverse conditions. The molecular mechanism underlying H2S action relies on its chemical reactivity, and the most-well characterized mechanism is persulfidation, which involves the modification of protein thiol groups, resulting in the formation of persulfide groups. This modification causes a change of protein function, altering catalytic activity or intracellular location and inducing important physiological effects. H2S cannot react directly with thiols but instead can react with oxidized cysteine residues; therefore, H2O2 signaling through sulfenylation is required for persulfidation. A comparative study performed in this review reveals 82% identity between sulfenylome and persulfidome. With regard to abscisic acid (ABA) signaling, widespread evidence shows an interconnection between H2S and ABA in the plant response to environmental stress. Proteomic analyses have reve...
Sulfide promotes tolerance to drought through protein persulfidation in Arabidopsis
Journal of Experimental Botany
Hydrogen sulfide (H2S) is a signaling molecule that regulates essential plant processes. In this study, the role of H2S during drought was analyzed, focused on the underlying mechanism. Pretreatments with H2S before imposing drought on plants significantly improved the characteristic stressed phenotypes under drought and decreases the levels of typical biochemical stress markers such as, anthocyanin, proline and hydrogen peroxide. H2S also regulated drought-responsive genes, amino acid metabolism, and repressed drought-induced bulk autophagy and protein ubiquitination, demonstrating the protective effect of H2S pretreatments. Quantitative proteomic analysis identified 887 significantly different persulfidated proteins in plants under control and drought stress. Bioinformatic analyses of the proteins more persulfidated in drought revealed that the most enriched biological processes were cellular response to oxidative stress and hydrogen peroxide catabolism. Protein degradation, abiot...
Hydrogen Sulfide: A Novel Gaseous Molecule for Plant Adaptation to Stress
Journal of Plant Growth Regulation, 2021
Hydrogen sulfide (H 2 S) has emerged as a novel gaseous signal molecule with multifarious effects on seed germination, plant growth, development, and physiological processes. Due to its dominant role in plant stress tolerance and cross-adaptation, it is getting more attention nowadays, although it has been largely referred as toxic and environmental hazardous gas. In this review work, we are highlighting the importance of H 2 S as an essential gaseous molecule to help in signaling, metabolism, and stress tolerance in plants. Firstly, production of H 2 S from different natural and artificial sources were discussed with its transformation from sulfur (S) to sulfate (SO 4 2−) and then to sulfite (SO 3 2−). The importance of different kinds of transporters that helps to take SO 4 2− from the soil solution was presented. Mainly, these transporters are SULTRs (H + /SO 4 2− cotransporters) and multigene family encodes them. Furthermore, these SULTRs have LAST (Low affinity transport proteins), HAST (High affinity transport proteins), vacuole transporters, and plastid transporters. Since it is well known that there is strong relationship between SO 4 2− and synthesis of hydrogen sulfide or dihydrogen sulfide or sulfane in plant cells. Thus, cysteine (Cys) metabolism through which H 2 S could be generated in plant cell with the role of different enzymes has been presented. Furthermore, H 2 S in interaction with other molecules could help to mitigate biotic and abiotic stress. Based on this review work, it can be concluded that H 2 S has potential to induce cross-adaptation to biotic and abiotic stress; thus, it is recommended that it should be considered in future studies to answer the questions like what are the receptors of H 2 S in plant cell, where in plants the physiological concentration of H 2 S is high in response to multiple stress and how it induces cross-adaptation by interaction with other signal molecules.
Hydrogen Sulfide: A Robust Combatant against Abiotic Stresses in Plants
Hydrogen
Hydrogen sulfide (H2S) is predominantly considered as a gaseous transmitter or signaling molecule in plants. It has been known as a crucial player during various plant cellular and physiological processes and has been gaining unprecedented attention from researchers since decades. They regulate growth and plethora of plant developmental processes such as germination, senescence, defense, and maturation in plants. Owing to its gaseous state, they are effectively diffused towards different parts of the cell to counterbalance the antioxidant pools as well as providing sulfur to cells. H2S participates actively during abiotic stresses and enhances plant tolerance towards adverse conditions by regulation of the antioxidative defense system, oxidative stress signaling, metal transport, Na+/K+ homeostasis, etc. They also maintain H2S-Cys-cycle during abiotic stressed conditions followed by post-translational modifications of cysteine residues. Besides their role during abiotic stresses, cr...
Hydrogen sulfide: A versatile gaseous molecule in plants
Plant Physiology and Biochemistry, 2021
Hydrogen sulfide (H 2 S) is a gasotransmitter and signaling molecule associated with seed germination, plant growth, organogenesis, photosynthesis, stomatal conductance, senescence, and post-harvesting. H 2 S is produced in plants via both enzymatic and non-enzymatic pathways in different subcellular compartments. Exogenous application of H 2 S facilitates versatile metabolic processes and antioxidant machinery in plants under normal and environmental stresses. This compound interacts with phytohormones like auxins, abscisic acid, gibberellins, ethylene, jasmonic acid, and salicylic acid. Furthermore, H 2 S participates in signal transductions of other signaling molecules like nitric oxide, carbon monoxide, calcium, methylglyoxal, and hydrogen peroxide. It also mediates post-translational modification, which is a protective mechanism against oxidative damage of proteins. This review summarizes the roles of H 2 S as intriguing molecule in plants.
Hydrogen Sulfide as a Potent Regulator of Plant Responses to Abiotic Stress Factors
Molecular Approaches in Plant Abiotic Stress, 2013
Adverse abiotic stress conditions such as drought, salinity, cold, heat and heavy metal toxicity are considered to be major concerns of the agricultural industry worldwide, as they can affect crop yield and quality, as well as agricultural sustainability in general. Priming of crop plants for more rapid and robust activation of defence reactions offers a means for the effi cient alleviation of the devastating effects induced as a result of exposure to such adverse environmental conditions. Accumulating reports over the recent years postulate a role for hydrogen sulfi de (H 2 S) as an emerging signaling molecule involved in the regulation of physiological processes in plants. In this chapter, we provide a brief overview of recent literature concerning H 2 S biosynthesis and regulation within the plant cell, as well as its involvement in a series of plant physiological processes. Furthermore,