Effects of Bradyrhizobium japonicum on Nitrogen Content in Soybean Leaves and Seeds Cultivated on Acidic Soils (original) (raw)
Related papers
American Journal of Agricultural and Biological Sciences, 2022
The legume-rhizobium symbiosis plays an important role in Nitrogen (N) uptake by plants, more particularly in the humid tropical region where soils are deeply weathered and have poor electrochemical properties. However, the influence of Bradyrhizobium on N allocation in soybean organs is not clearly understood. Here, we assessed the effect of Bradyrhizobium japonicum on N concentration of soybean leaves and seeds cultivated on acidic soils. We conducted two experiments using a randomized full device with three replicates in two sites (i.e., Kasapa and Kanyameshi). The main plots included three strains of Bradyrhizobium and the uninoculated control while the subplots included soybean variety. We found that different strains of Bradyrhizobium did not induce significant effects on total N concentration in soybean leaves and seeds in the Kasapa site. In contrast, Bradyrhizobium strains affected significantly N concentration in soybean leaves in the Kanyameshi site. We demonstrated that soil N concentration, which varies between investigated sites, positively influences yield and nodulation. We conclude that the efficiency of the soybean-Bradyrhizobium symbiosis and its influence on the allocation of N through soybean organs strongly depend on the chemical characteristics of soil and particularly on the initial N concentrations in soils.
Pesquisa Agropecuária Tropical
In early soybean cultivars of high productive potential, the use of chemical nitrogen (N) fertilizer may be a critical factor to meet the crop N requirements for obtaining high yields. In order to determine the response of early soybean cultivars to doses and times of nitrogen fertilizer application, associated with the inoculation of Bradyrhizobium japonicum, two field experiments were conducted in a Quartzipsamment soil from the Brazilian tropical Savannah, during the 2016/2017 and 2017/2018 growing seasons. The experimental treatments were arranged in a 2 × 3 × 4 factorial scheme, being two soybean cultivars (BRS 1074 IPRO and ST 797 IPRO), three application times of N fertilizer (sowing, 30 and 50 days after the emergence) and four N doses (0 kg ha-1, 20 kg ha-1, 40 kg ha-1 and 60 kg ha-1). The following variables were evaluated: plant height, shoot dry matter, number of nodules, nodule dry matter, first pod height, number of pods, number of grains per pod, 1,000-grain mass, gra...
Journal of Central European Agriculture, 2008
In this study the symbiotic interactive effect of different Bradyrhizobium japonicum strains with six soybean cultivars were evaluated under fi eld conditions. The rhizobial strains ASR011, USDA123 and CB1809 respectively showed hostcultivar specifi city with JS335, Lee and Bragg. B. japonicum ASR011 recorded the highest nodulation and nitrogenase activity with all the studied cultivars. Generally, plants inoculated with strain ASR011 produced higher plant dry matter accumulation and seed yield over all other cultivars. On the basis of analysis of correlation coeffi cients, it was found that plant dry matter accumulation emerged as best criterion for selection of most effective legume-Rhizobium associations for given physical and biological conditions.
Revista Brasileira de Ciências Agrárias - Brazilian Journal of Agricultural Sciences, 2021
The goal of this study was to evaluate the efficiency of Bradyrhizobium brasilense strains native to the soil of the semiarid region in northeastern Brazil in symbiosis with soybeans in two contrasting soils in southwestern Piauí. A pot experiment was conducted using a randomized block design in a 10 × 2 factorial scheme. There were 10 nitrogen (N) sources: six native Bradyrhizobium strains [UFLA06-13, UFLA06-15, UFLA06-19, UFLA06-21, UFLA06-22 (B. brasilense), and UFLA06-24 (Bradyrhizobium sp.)], two controls with strain SEMIA 5019 (B. elkanii) and a commercial inoculant [SEMIA 5079 (B. japonicum) + SEMIA 5080 (B. diazoefficiens)] recommended for soybeans, and two controls without the application of an inoculant (one with and the other without the application of mineral N). The second experimental factor corresponded to the use of two soils (Oxisol and Quartzarenic Neosol). All strains showed increased nodulation and shoot nitrogen content in soybean plants in both soils. Most strains promoted higher nitrogen fixation when inoculated in the Oxisol. UFLA06-19, UFLA06-22, and UFLA06-24 were efficient in nitrogen accumulation in the shoots of soybeans in the Oxisol. This is the first report regarding the efficiency of B. brasilense strains in symbiosis with soybeans under different soil conditions.
Soil Biology and Biochemistry, 1996
There are few reports from Indonesia of responses by soybean [Glycine mux (L.) Merr.] to inoculation with Bradyrhizobium japonicum. In only one, and that at only one site, was the response expressed in terms of both the proportion of nodules formed by the inoculum and seed yield . In their experiments, although 50% of the nodules were formed by the inoculum. there was no corresponding increase in seed yield. Pasaribu et al. (1989) sowed local soybean lines inoculated with compatible strains of B. juponicum at 11 sites but only at one Biochemistry 7, 30553 1 I Indonesia. Soil Biology & Biochemistry 27, 625-628.
Soil Biology and Biochemistry, 2010
The 15 N natural abundance technique is one of those most easily applied 'on farm' to evaluate the contribution of biological N 2 fixation (BNF) to legume crops. When proportional BNF inputs are high, the accuracy of this technique is highly dependent on an accurate estimate of the 15 N abundance of the N derived from N 2 fixation (the 'B' value). The objective of this study was to determine the influence of soybean variety on 'B' value. Plants of five soybean varieties were inoculated separately with two Bradyrhizobium strains (one Bradyrhizobium japonicum and one Bradyrhizobium elkanii) grown in pots of soil virtually free of bradyrhizobia capable of nodulating soybean. The proportion of N derived from BNF (%Ndfa) was estimated in separate pots where a small quantity of enriched 15 N ammonium sulphate was added. The %Ndfa was then used with the 15 N natural abundance data of the nodulated soybean and non-N 2-fixing reference plants, to determine the 'B' value for each soybean variety/Bradyrhizobium association. The varieties nodulated by the B. japonicum strain showed significantly greater N content and %Ndfa than those nodulated by the B. elkanii strain, and in all cases the 'B' value of the shoot tissue ('B s ') was higher. The differences in 'B s ' values between varieties nodulated by the same Bradyrhizobium strain were insignificant, indicating that this parameter is influenced much more by the Bradyrhizobium strain than by the variety of the host plant.
2012
Influence of starter nitrogen (N) and phosphorus (P) applications on mycorrhizal colonization and nodulation of soybean (Glycine max (L.) Merill) was investigated at two sites of humid forest margins of Cameroon. Four soybean varieties, TGX 1838-5E (var1), TGX 1879-7E (var2), TGX 1828-4E (var5) and TGX 1805-13F (var6), were grown for four months in fields from different fallow ages, in a factorial combination of 30 kg N.ha-1 and 30 kg P.ha-1, with five replicates, in a randomized complete block design. Soil acidity varied strongly with site, being 5.9 and 4.5, at Nkometou and Mengomo, respectively. Fractional mycorrhizal colonization (FMC) was not affected by soil pH. FMC significantly varied among fields between 20% and 40%, was significantly reduced by P fertilization while effect of N amendment was contrasted. Nodulation was strongly influenced by soil pH: high nodulation in Nkometou but extremely low in Mengomo. In Nkometou, early maturing soybean varieties (var2 and var5) yield...
2016
Biological nitrogen fixation (BNF) is an essential part of sustainable agriculture. Inorganic nitrogenous fertilizers are extensively used in modern, high input agriculture. However, they are expensive, and poorly absorbed by plants so much of the nitrogen that is applied is lost due to leaching, which results in soil acidification and ground water pollution. In contrast, rhizobia have the capacity to fix atmospheric nitrogen effectively, resulting in the uptake and assimilation of nitrogen into plants without the harmful environmental and related application costs. This study aimed to isolate, screen and identify freshly isolated Bradyrhizobium spp. and Rhizobium spp. for multi-host plant growth promotion. Freshly isolated rhizobial isolates were obtained from the nodules on roots of soybean (Glycine max (L.) Merrill), cowpea (Vigna unguiculata (L.) Walp.), pigeonpea (Cajanus cajan (L.) Millsp.) and groundnut (Arachis hypogaea (L.) Kohler). Thirty-three isolates were isolated and stored in 40% glycerol solution at-80°C. Two commercial multi-host soybean strains, XS21 and WB74, to be used as controls, were obtained from the Agricultural Research Council, Mpumalanga, South Africa, in subsequent trials. The development of visual growth scales of each of the test crops [soybean, cowpea, dolichos (Lablab purpureus (L.) Sweet) and dry bean (Phaseolus vulgaris (L.) var. Gadra)], were developed in order to study the dynamics of nitrogen fluxes that occurred in the growth cycles of each of the crops. The total chlorophyll and leaf nitrogen contents were determined, based on the chlorophyll and leaf nitrogen content at specific growth stages, for each crop, at multiple levels of nitrogen fertilization. A linear correlation was observed between the total chlorophyll content and that of the leaf nitrogen content of each test crop except for soybean. However, due to physiological crop damage due to red spider mite (Tetranychus urticae Koch.), powdery mildew (Erysiphe polygoni D.C.) and mealy bug (Pseudococcus filamentosus Guen.) infestations, that caused yellowing leaves, there was no linear correlation between chlorophyll content and leaf nitrogen content for soybean. There were significant differences in total chlorophyll content for dolichos and dry bean crops and for total leaf nitrogen content between the soybean, cowpea and dry bean crops. The thirty-three freshly isolated rhizobial strains and the two commercial multi-host soybean strains (WB74 and XS21) were tested in vitro with for three secondary plant growth promoting traits: inorganic phosphate solubilization, siderophore production and indole-3-Page-3-of 194 Chapter 5: Screening of 31 freshly isolated strains of Rhizobium and Bradyrhizobium spp. on four legume crops for multi-host proficiency by measuring the chlorophyll content