Pengembangan Metode Analisis Chlorampenicol Secara Spektrofotometri Uv-Vis Berbasis Reaksi Diazotasi Pada Suhu Dingin (original) (raw)

2021, PROSIDING SEMINAR KIMIA

Development of chlorampenicol analytical method UV-VIS spectrophotometry based on the diazotation reaction at cold temperatures has been carried out. In this study, a coupling reaction was carried out between diazonium salt sourced from sulfanilamide and chlorampenicol, diazonium salt was made by reacting sodium nitrite and HCl with sulfanilamide. In the manufacturing process and the coupling reaction between diazonium salt and chlorampenicol was carried out at cold temperatures and the resulting compound was stable at ± 10°C. The compound formed was an azo sulfanilamide-CAP compound and was measured by UV-Vis spectrophotometry at a wavelength of 400,941 nm.

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