Transgenic allele frequencies among Carb109/GDLS backcrossed mosquitoes over five generations (F₁–F₅) in absence of selection for the transgenic phenotype (original) (raw)

2014

Abstract

<p>Initial frequencies (p₀) of the Carb109 transgene were either (A) 0.5 (transgenic heterozygote x transgenic heterozygote) for Carb109F/GDLS.BC₁, Carb109M/GDLS.BC₁, Carb109F/GDLS.BC₅, and Carb109M/GDLS.BC₅ or (B) 0.25 (transgenic heterozygote x GDLS) for Carb109F/GDLS.BC₁, Carb109M/GDLS.BC₁, Carb109F/GDLS.BC₅, and Carb109M/GDLS.BC₅. Fifteen lines were established for each of the eight experiments. Proportions of EGFP-expressing offspring were estimated by examining ∼150 larvae from each of the 15 lines over five successive generations (F₁–F₅) of inter-breeding without selection for the transgenic phenotype. Bars around mean proportions represent Bayesian 95% Highest Density Intervals (95% HDI). Proportions showing non-overlapping 95% HDI are credibly different.</p

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