Antioxidant Properties of Nine Commonly Used Medicinal Plants in Arabs Region (original) (raw)
Related papers
2015
The using of plants of Saudi Arabia for the cure of many ailments is ancient and still available among the tribal and local people and medicinal healers. In this study, alcoholic extract of seven plants from Tanhat protected area, Saudi Arabian, were studied for their antioxidant properties. The antioxidant properties were evaluated using different antioxidant tests, including inhibition of lipid peroxidation, reducing power, metal ion chelation, scavenging of free radical, superoxide anion radical, hydrogen peroxide and nitric oxide as well as total antioxidant capacity. Trigonella hamosa and Ducrosia anethifolia had powerful antioxidant activity, had 100% inhibition on peroxidation of linoleic acid emulsion at 200 µg/ml while Cleome ambliocarpa was the most efficient as scavenger. The presence of phenolic compounds and flavonoids in the extracts, which were detected by Folin-Ciocalteu may be responsible for the antioxidant activities of Heliotropium ramosissimum which exhibited the highest reduction capability with highest polyphenolic content (48mg/100mg extract as chlologenic acid). Moreover, Trigonella hamosa and Convolvulus prostates had effective metal chelating activity (100% ion chelation at 100µg/ml). Those various antioxidant activities were compared to standard antioxidants such as butylatedhydroxytoluene (BHT) and vitamin C.
In this study, antioxidant activity, total phenolic and total flavonoid contents of 30 species extracts, from 15 botanical families grown on three localities (Agdz, Tafraout and Taroudannt) of Souss Massa Draa (Southern Morocco), were determined using spectrophotometric methods. DPPH radical cation assay was used for evaluation of free radical scavenging properties of investigated species. The results showed a remarkable high antioxidant activity in the majority of tested plants, especially in Tetraclinis articulata, Thymus leptobotrys, and Lavandula stoechas, which neutralized up to 90 % of DPPH radicals. However, Conyza canadensis showed the lowest antioxidant value (88.19%). The total phenolics content measured by Folin-Ciocalteu method, ranged from 2.54 to 55.62 µg of gallic acid equivalents (GAE)/mg of dry matter (DM). Ceratonia siliqua (55.63 µg GAE /mg), Cistus villosus (41.10 µg GAE /mg), Limoniastrum feei (36.52 µg GAE /mg) and Rubus ulmifolius (35.02 µg GAE /mg) had very high levels of phenolics, whereas in Conyza canadensis (2.54 µg GAE /mg) phenolics were quit low. For total flavonoids content, two methods were used. Spectrophotmoetric method using NEU reagent showed a significant levels of flavonoids in Inula viscosa (70.08 µg/mg), Globularia alypum (66.28 µg/mg), Teucrium chamaedrys (63.95 µg/mg), Ruta montana (59.9 µg/mg) and Ononis natrix (55.49 µg/mg). For the second method with aluminium trichloride (AlCl3), the amounts of total flavonoid expressed as rutin equivalents/g DM, were higher in Rubus ulmifolius (117.79 µg/mg), Ononis natrix (80.84 µg/mg), Rhus pentaphulla (67.67 µg/mg), Thymus leptobotrys (53.17 µg/mg) and Thymus satureioides (52.34 µg/mg). According to this method Mentha pulegium exhibited the lowest flavonoids concentration (9.46 µg/mg), while the first method revealed low levels of flavonoids content in Conyza canadensis (5.41 µg/mg).
Assessment of antioxidant activities of three wild medicinal plants from Bahrain
Arabian Journal of Chemistry, 2015
This study reports the antioxidant properties of three wild medicinal plants from Bahrain, namely Aizoon canariense L., Asphodelus tenuifolius Cav., and Emex spinosus (L.) Campdera. Antioxidant and antiradical activities of dried materials of these plants were investigated using FRAP, DPPH and ABTS assays. Total phenolics, free phenolics and total flavonoids were also determined. E. spinosus was ranked by the assays as the plant possessing the highest antioxidant and antiradical activities with an average FRAP value of 1.84 mmol/g and IC 50 of 10.7 and 7.75 mg/ml for DPPH and ABTS assays, respectively. A. tenuifolius ranked second with a mean FRAP value of 0.69, IC 50 DPPH of 1.72 and ABTS of 0.36. A. canariense possessed the lowest activities with a mean FRAP value of 0.6, and averaged IC 50 of 103.8 and ABTS of 14.6. E. spinosus possessed the highest content of free phenolics (mg/100 g) (64.64) followed by A. tenuifolius (45.21) and A. canariense (32.23). E. spinosus also exhibited the highest total flavonoids with an average 82.71 mg/100 g followed by A. canariense (55.92) and A. tenuifolius (49.10). The studied medicinal plants possess considerable antioxidant activities and may contribute to the well-being of individuals who consume them.
Review on some antioxidant plants growing in Arab world
Journal of Saudi Chemical Society, 2011
Cellular damage or oxidative injury arising from free radicals or reactive oxygen species (ROS) now appears the fundamental mechanism underlying a number of human neurodegenerative disorders, diabetes, inflammation, viral infections, autoimmune pathologies and digestive system disorders. Free radicals are generated through normal metabolism of drugs, environmental chemicals and other xenobiotics as well as endogenous chemicals, especially stress hormones (adrenalin and noradrenalin). Accumulated evidence suggests that ROS can be scavenged through chemoprevention utilizing natural antioxidant compounds present in foods and medicinal plants. Plant extracts and their constituents as a natural source of antioxidants have been extensively reviewed. Plant extracts containing low molecular mass compounds have been successively used in phytotherapy since ancient times, as reactive oxygen species are involved in several diseases in this review, research on the antioxidant potential of medicinal plants.
2007
As part of an International Cooperative Biodiversity Groups (ICBG) program to study Jordan's biodiversity, the relative levels of antioxidant activity and the total phenolic content of aqueous and methanolic extracts of a total of 95 plant species, all of Jordanian origin and those collected at random, have been measured. The total phenolic content of aqueous and methanolic extracts of the investigated plant species ranged from 4.4 to 78.3 mg and from 2.1 to 52.8 mg gallic acid equivalents g−1 dry weight, respectively, while the total antioxidant capacity ranged from 20.0 to 916.7 and from 15.1 to 915.6 μmol Trolox equivalents g−1 dry weight, respectively. Based on this collection, approximately 5% of assayed plants showed high levels of antioxidant activity. There was a significant linear correlation between antioxidant activity and total phenolic content for aqueous and methanolic extracts, suggesting that phenolic compounds were the predominant antioxidant components in the i...
2012
Several herbal plants have been used as therapeutics in Iraqi traditional medicine and phenolic content and antioxidant activity may contribute to their pharmacological effects. The total phenolic contents (TPC) and the antioxidant activities of water and ethanol extracts of 14 medicinal plants which had been used in Iraqi traditional medicine were investigated. The TPC were measured by Folin-Ciocalteu method. The antioxidant activity was assessed by ferric reducing antioxidant power (FRAP) assay and the scavenging activity towards 2, 2′-diphenyl-1-picryl hydrazyl (DPPH)-radical. Antioxidant activities as measured by FRAP and the scavenging activity towards DPPH radical was significantly correlated with TPC among all the plants studied. In most of the plants the TPC and the antioxidant activity of the ethanol extracts were significantly higher (P< 0.05) than that of water alone extracts. The results suggest that phenolic compounds are the significant contributors to the antioxida...
Aantioxidant Activity and Phenoliccontent of Some Emirates Medicinal Plants
Plant antioxidants have profound importance due to their biological activities against reactive oxygen species. In this research, nine hydroalcoholic extracts of traditionally medicinal plants from the Arabian Gulf region were evaluated for their antioxidant properties and polyphenol contents. The total antioxidant property was estimated by the ferric reducing antioxidant power (FRAP) assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH·) assay. The total antioxidant activity, measured by FRAP assay, ranged from 11.9 to 70.3 µmol ascorbic acid equivalents/g dry plant. The free radical scavenging activity, measured by DPPH·assay ranged from 0.56 to 253.5 µg/ml (effective concentration, EC 50 ). The total phenolic content of plant extracts was measured using the Folin-Ciocalteu assay. The total phenolic contents varied between 2.96-15.1 mg gallic acid equivalents/g dry plant. Plants with the highest total antioxidant capacities and phenolic contents were Fagonia ovalifolia and Heliotropium bacciferum. With further data analysis, it was found that there was a positive correlation between the total phenolic content of plants and their FRAP values (R 2 = 0.865). These results suggest that the presence of phenolic compounds contributes significantly to the antioxidant activity of these plants. Flora of the Gulf appears to be a rich source for ethnomedicinal and phytochemical studies.
Screening of antioxidant activity and phenolic content of 24 medicinal plant extracts
DARU Journal of Pharmaceutical …, 2008
Background: Antioxidants are vital substances which possess the ability to protect the body from damages caused by free radical-induced oxidative stress. A variety of free radical scavenging antioxidants are found in dietary sources like fruits, vegetables and tea. The purpose of this study was to evaluate the antioxidant activity of methanolic extracts of 24 selected plant materials (seeds or fruits), which are used by Iranian people as folk remedies and/or food supplements. Methods: The antioxidant activity was evaluated against linoleic acid peroxidation using 1,3-diethyl-2-thiobarbituric acid as reagent. At the same time the phenolic content of the extracts was determined using Folin-Ciocalteau reagent to evaluate their contribution to total antioxidant activity. Results: The antioxidant activity expressed as IC 50 ranged from 1.25 µg/ml in cucumber to 167.29 µg/ml in cardamom. Phenolic contents, expressed as gallic acid equivalents, varied from 21.76 mg/100g of the dried weight in linseed to 919.12 mg/100 g of the dried weight in Bishop's weed. No significant correlation was observed between antioxidant activity and phenolic content in the studied plant materials. Conclusion: The results of this study showed that there is no significant correlation between antioxidant activity and phenolic content of the studied plant materials and phenolic content could not be a good indicator of antioxidant capacity.
The environmental stresses such as high or cold temperatures, heat, UV irradiation and infection by pathogen stimulate the production of secondary metabolic products that have medicinal values and antioxidant properties such as phenolics and flavonoids. This study aimed to investigate the effect of seasonal variations of climatic conditions on phenolic and flavonoid compounds, as well as antioxidant capacity of three plant species belong to different families growing naturally in Wadi Halazein. The investigated plants were Seriphidium herba-alba, Teucrium polium and Peganum harmala. The results indicated that the total contents of phenolics and flavonoids was significantly affected by species and season and tended to increase during the climatic condition of summer season. The results of DPPH assay (radical scavenging) and FARP assay (ferric-reducing antioxidant power), indicated that under the climatic conditions of summer season, Serphedium herba alba showed the highest antioxidant capacity followed by T.polium and P.harmala. The ability of their ethanolic extracts to scavenge free radicals and chelate metals (ferric ion), indicated that phenolic and flavonoid compounds were the main compounds that contributed to antioxidant activity of the investigated plants. The HPLC analysis indicated that the highest values of medicinal compounds that have high antioxidant activity and high medicinal values, such as rutin (1.303 mg g-1), quercetin (2.527 mg g-1), apigenin 7-glucose (0.798 mg g-1), kaempferol (1.644 mg g-1) and apigenin (0.175 mg g-1) were detected in the extract of Serphedium herba alba under the climatic conditions of summer season. Whereas, the highest content of naringin was detected in winter sample of Serphedium herba alba, while the high content of naringenin (4.012 mg g-1) was detected in summer sample of P.harmala. This study supports using these plants as a source of natural antioxidant compounds especially Serphedium herba alba, which contains valuable antioxidant compounds that have anti-cancer and inflammatory activities.
Phytochemical Screening and Antioxidant Activity of some Egyptian Medicinal Plants
Journal of Modern Research
Extracts of three leaves: Cymbopogon citratus, Hibiscus sabdariffa, and Lawsonia inermis were screened for phytochemicals in ethanol (80%), hexane, and ethyl acetate. The potential of plant extracts to scavenge free radicals such as DPPH, ABTS and FRAP (Ferric Reducing Antioxidant Power) was investigated. For all extracts, phytochemical analysis indicated the presence of terpenoids, steroids, tannins, anthocyanins, phenols and flavonoids with absence of fatty acids in ethanol 80% and ethyl acetate. Ethyl acetate extract of L. inermis achieved a high percentage of TPC and TFC, but it was higher in 80% ethanol of H. sabdariffa than the other extracts under study. Ethyl acetate extracted the highest TPC (66.8 ± 3.6 mg/100g) in C. citratus and 80% ethanol extracted the highest TFC (37.23± 2.05mg/100g). Ethanolic extract (80%) of the three plants' leaves showed a higher IC 50 value than both ethyl acetate and hexane extracts. This indicates that 80% ethanol was the best solvent for extracting phenolic compounds and flavonoids. The "ABTS radical" scavenging activity was the highest for ethyl acetate extract of L. inermis. The lowest value was observed for hexane extract of both C. citratus and L. inermis. This study exhibited potential of ethanolic extracts (80%) of all plants for removing free radicals and their effectiveness to be a powerful antioxidant.