blackspot seabream Pagellus bogaraveo (Brünnick (original) (raw)
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Aquaculture …, 2006
Andreia Lemos1, Ana Isabel Freitas2, Ana Teresa Fernandes2, Rita Gonc°alves2, Jose¤ Jesus1, Carlos Andrade3 & Anto¤nio Brehm2 1Department of Biology, University of Madeira, Funchal, Portugal 2Human Genetics Laboratory, University of Madeira, Funchal, ...
Aquaculture Research, 2006
Eight microsatellite loci previously reported were assessed for their utility in parentage assignment in 96 individuals belonging to natural populations of the blackspot seabream Pagellus bogaraveo (Brˇnnick, 1768) from the Mediterranean and Northeast Atlantic regions. At the mtDNA level, no di¡erentiation was found between these two regions but based on microsatellite data an overall discrete genetic di¡erentiation is perceivable between the two regions separated by the Strait of Gibraltar. The number of alleles per locus ranged from 8 to 30. A database constructed with allele frequency data from six populations was used in a simulation parentage assignment test using the software CERVUS. The test showed that the number of markers used is enough to perform parentage assignments with real data. The polymorphic information content for each locus was very high (mean value of 0.849), with a total exclusionary power of 0.9995. In summary, seven of the eight microsatellites analysed proved to be su⁄cient and powerful tools for parentage assignment in hatcheries and the allele frequency data given here can be used to perform pedigree analysis against which real data may be tested.
Marine Biology, 2005
The depletion of shallow-water fish stocks through overexploitation has led to increasing fishing pressure on deep-sea species. Poor knowledge of the biology of commercially valuable deep-water fish has led to the serial depletion of stocks of several species across the world. Data regarding the genetic structure of deepsea fish populations is important in determining the impact of overfishing on the overall genetic variability of species and can be used to estimate the likelihood of recolonisation of damaged populations through immigration of individuals from distant localities. Here the genetic structure of the commercially fished deep-water species the blackspot sea bream, Pagellus bogaraveo is investigated in the northeastern Atlantic using partial DNA sequencing of mitochondrial cytochrome b (cyt-b) and D-loop regions and genotyping of microsatellite loci. An absence of variation in cyt-b and low genetic variation in D-loop sequences potentially indicate that P. bogaraveo may have undergone a severe bottleneck in the past. Similar bottlenecks have been detected in other Atlantic species of fish and have possibly originated from the last glaciation. P. bogaraveo may have been particularly vulnerable to the effects of low temperature and a fall in sea level because stages of its life history occur in shallow water and coastal sites. However, there are other explanations of low genetic variability in populations of P. bogaraveo, such as a low population size and the impacts of fishing on population structure. Analysis of population structure using both D-loop and microsatellite analysis indicates low to moderate, but significant, genetic differentiation between populations at a regional level. This study supports studies on other deep-sea fish species that indicate that hydrographic or topographic barriers prevent dispersal of adults and/or larvae between populations at regional and oceanographic scales. The implications for the management and conservation of deep-sea fish populations are discussed.
Fisheries Science, 2003
Four highly variable microsatellite loci, Acs1*, Acs3*, Acs4* and Acs9*, were isolated and then used to investigate genetic diversity and population structure in black sea bream, Acanthopagrus schlegeli, collected from wild populations of six locations (western Japan and southern Korea), / and hatchery stock and post-stock populations from Hiroshima Bay. When allelic segregation of each .' microsatellite locus was examined using the single spawning pair and their progeny, all loci were well fitted to the Mendelian manner of inheritance. The genetic variations at these loci in the eight populations revealed high levels of variability. In the wild populations the mean number of alleles per locus was 10.8-13.5, and the mean observed hetrozygosity was 0.755-0.828. In contrast, these values in hatchery stock population were 10.0 and 0.776, respectively. Genetic distance indicates the existence of geographic divergence between western Japan and south Korea, and little genetic differentiation among populations in western Japan.
Microsatellite polymorphisms in wild populations of European seabass: Preliminary results
1998
SUMMARY-Studies on population genetics of the European seabass (Dicentrarchus labrax) have shown the existence of extensive allozyme variation particularly between populations in different sea basins. The migration behaviour of juvenile and adult bass and the low levels of differentiation observed between samples in the same hydrographic area has lead to the assumption of a panmixia over quite large areas of the species range.
1996
Allelic variation in six highly polymorphic microsatellite loci (mean heterozygosity, 86%) provided evidence that cod (Gadus morhua) in the northwest Atlantic belong to multiple genetically distinguishable populations, and further that genetic differences may also exist between northwestern and southeastern cod aggregations within the northern cod stock complex off Newfoundland. Cod were sampled from winter aggregations ranging from Hamilton Bank to the northern Grand Bank in the northern cod complex, and from the Flemish Cap, the Scotian Shelf, and the Barents Sea. Tests of allele frequency homogeneity (χ 2 ), F ST , and (δµ) 2 , allele sharing, and Rogers' distance measures revealed significant differences among northern cod, Flemish Cap, Scotian Shelf, and Barents Sea samples. Within the northern cod complex, two pooled samples, NORTH (Hamilton, Funk, and Belle Isle banks) and SOUTH (northern Grand Bank area), were distinguishable using χ 2 , (δµ) 2 , and allele sharing measures. Both (δµ) 2 and Rogers' distances clustered western Atlantic samples in two groups distinct from the divergent Barents Sea sample; one comprised NORTH, SOUTH, and Scotian Shelf, and the other, Flemish Cap.
Molecular Ecology …, 2006
The redbanded seabream Pagrus auriga (Teleostei, Sparidae) is a species of a high commercial value in Spain. There is currently little information available about the genetic characteristics of both wild and cultured populations. In this survey, we have developed eight polymorphic microsatellites for the redbanded seabream using an enriched genome library protocol. All of them were polymorphic in the 64 individuals tested, 22 of which were wild specimens, and 42 were individuals from a captive reproductive broodstock. These markers can potentially be useful tools for use in population genetic studies.
Microsatellite multiplex panels for population genetic analysis of red sea bream Pagrus major
Fisheries Science, 2012
In this study, multiplex PCR panels were developed to amplify 20 microsatellite markers for red sea bream, Pagrus major, with the aim of reducing labor and material costs associated with genetic analysis of this species. The usefulness of these panels was validated in 200 fish collected at five sampling locations. The occurrence of null alleles was suggested at five markers, which were dropped from further analysis. The remaining 15 microsatellite loci showed high levels of heterozygosity (H E range 0.34-0.97, H O range 0.32-1) and a wide range in the number of alleles per locus (A; range 5-46). Increasing the number of microsatellite loci from three to ten and 15 improved the performance of the panels for population differentiation (F ST) and estimation of Nei's (D S) genetic distance. The results of this study confirm the usefulness of genotyping a large number of microsatellite DNA markers to expand our knowledge of red sea bream population genetics.