Three distinct gonococcal genes related to H8 antigens (original) (raw)

Gonococci and Meningococci, 1998

Abstract

The λgtll expression vector was used to construct a gene bank of gonococcal DNA of strain RIO (serovar PIB-9). Upon screening with a rabbit antiserum containing antibodies to the H8 antigen a number of clones were isolated which produced products related to H8 antigen. DNA hybridization studies demonstrated that these clones were derived from three distinct gonococcal genes. The antigens produced by the clones were used to isolate antibodies from rabbit antiserum. Immunoblots of gonococcal lysates analyzed with the affinity purified antibodies and with H8 monoclonal antibody demonstrated that two of the H8 genes were expressed by the gonococcal strain studied and gave rise to products with apparent molecular weights of ca. 19,700 and 21,200.

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