Mechanistic Approach on the Pulmonary Oxido-Inflammatory Stress Induced by Cobalt Ferrite Nanoparticles in Rats (original) (raw)
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In Vitro Evaluation of the Toxicity of Cobalt Ferrite Nanoparticles in Kidney Cell
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Amaç: Sert manyetik kobalt ferrit nanopartiküllerinin (CoFe 2 O 4-NP) dikkate değer özellikleri ve fizikokimyasal kararlılıkları farklı endüstri ve tıp alanlarında çeşitli uygulamalarda kullanılmalarına yol açmaktadır. CoFe 2 O 4-NP'lerin bazı toksik etkilere neden olduğu bildirilmiş olsa da böbrek üzerindeki etkileri hakkında ciddi bilgi eksikliği vardır. Bu çalışmada, CoFe 2 O 4-NPs'lerinin NRK-52E böbrek hücreleri üzerine toksik etki potansiyellerinin araştırılması amaçlanmıştır. Gereç ve Yöntemler: Partikül karakterizasyonu ve hücresel alım transmisyon elektron mikroskopu, dinamik ışık saçılma tekniği ve indüktif eşleştirilmiş plazma-kütle spektrometrisi ile gerçekleştirildi. Sonra, sitotoksisite MTT ve nötral kırmızı alım testi, genotoksisite comet tekniği ve apoptotik potansiyel propidyum iyodürlü Annexin V-FITC apoptoz tayini ile değerlendirildi. Bulgular: CoFe 2 O 4-NP'lere (39±17 nm) 100-1000 μg/mL arasında değişen konsantrasyonlarda 24 saat süre ile maruz bırakılan böbrek hücrelerinde hücre canlılığının etkilenmediği, ancak ≤100 μg/mL'de önemli ölçüde DNA hasarı meydana geldiği gözlenmiştir. Maruz kalan hücrelerde apoptotik veya nekrotik etki gözlenmedi. Sonuç: Elde edilen sonuçlara göre, CoFe 2 O 4-NP'ler çeşitli uygulamalarda güvenli kullanımı vaat etmektedir. Bununla birlikte, etki mekanizmalarının tam olarak anlaşılabilmesi için in vivo çalışmalara ihtiyaç vardır. Anahtar kelimeler: DNA hasarı, hücre ölümü, apoptoz, kobalt ferrit nanopartikülü Objectives: The remarkable properties of hard magnetic cobalt ferrite nanoparticles (CoFe 2 O 4-NPs) and their physicochemical stability lead to various applications in different industrial and medical fields. Although CoFe 2 O 4-NPs have been reported to cause toxic effects, there is a serious lack of information concerning their effects on the kidneys. In this study, it was aimed to investigate the toxic effects of CoFe 2 O 4-NPs on NRK-52E kidney cells. Materials and Methods: The particle characterisation and cellular uptake were determined using transmission electron microscopy, dynamic light scattering and inductively coupled plasma-mass spectrometry. Then, the cytotoxicity was evaluated by MTT and neutral red uptake assays, the genotoxicity by comet assay, and the apoptotic potentials by Annexin V-FITC apoptosis detection assay with propidium iodide. Results: After 24 h exposure to CoFe 2 O 4-NPs (39±17 nm), it was observed they did not affect the cell viability at concentration ranging from 100 to 1000 μg/mL, but significantly induced DNA damage at concentration ≤100 μg/mL. No apoptotic or necrotic effect was observed in the exposed cells. Conclusion: According to the results obtained, CoFe 2 O 4-NPs are promising for safe use in various applications. However, further in vivo studies are needed to fully understand their mechanisms of action.
Nanotoxicology, 2016
In spite of the great promises that the development of nanotechnologies can offer, concerns regarding potential adverse health effects of occupational exposure to nanoparticle (NP) is raised. We recently identified metal oxide NP in lung tissue sections of welders, located inside macrophages infiltrated in fibrous regions. This suggests a role of these NP in the lung alterations observed in welders. We therefore designed a study aimed to investigate the pulmonary effects, in mice, of repeated exposure to NP administered at occupationally relevant doses. We therefore chose four metal oxide NPs representative of those found in the welder's lungs: Fe2O3, Fe3O4, MnFe2O4 and CrOOH. These NPs were administered weekly for up to 3 months at two different doses: 5 μg, chosen as occupationally relevant to welding activity, and 50 μg, chosen as occupationally relevant to the context of an NP-manufacturing facility. Our results show that 3 month-repeated exposures to 5 μg NP induced limited...
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Journal of toxicologic pathology, 2013
Information about potential risks of iron nanomaterials is still limited, while a wide variety of applications are expected. We recently reported acute phase responses of male and female Fischer 344 rats after a single intratracheal spray instillation of Fe3O4 nanoparticles (magnetite), clearly showing dose-dependent pulmonary inflammatory changes (Tada et al., J Toxicol Pathol 25, 233-239, 2012). The present study assessed long-term responses of male and female Fischer 344 rats to multiple administrations of magnetite. Ten-week-old male and female Fischer 344 rats (n=20/group) were exposed to a total of 13 quadweekly intermittent intratracheal spray instillations of magnetite during the experimental period of 52 weeks, at doses of 0, 0.2 (low), 1.0 (medium) and 5.0 (high-dose) mg/kg body weight per administration. Absolute and relative lung weights of the high-dose group were significantly higher than those of the control group. Macroscopically, slight enlargement and scattered bla...
Nanotoxicology, 2012
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Nanoscale Research Letters, 2019
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