Bioprocessing and Screening of Indigenous Wastes for Hyper Production of Fungal Lipase (original) (raw)
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Energy Reports, 2020
Lipase is one of the most used industrial enzymes, which can be produced by Aspergillus niger. Large scale production of Aspergillus niger lipase is more profitable by using solid state fermentation method and utilizing agro-industrial waste as the substrate. Optimization of Aspergillus niger lipase production has been performed in this study. Optimization was performed on solid state fermentation of rice bran and Jathropa seed cake for 5 days with variations of inducer and extractant. Fermentation cake produced was extracted, filtered using muslin cloth, and centrifuged. The supernatant was spray-dried and assayed using olive oil hydrolysis titrimetry. Inducer optimization results showed that 1% of olive oil was the best inducer, yielding dry lipase extract with highest activity unit (176 U/ml enzyme). Extractant optimization results showed that 1% of NaCl-0.5% of Tween 80 was the best extractant, yielding dry lipase extract with highest activity unit (282 U/ml enzyme). c
Solid State Fermentation of Agricultural Residues for Lipase Production in a Tray-Bioreactor
World Applied Sciences …, 2012
The novel tray-bioreactor was designed and constructed to produce lipases with high activities. Fermentation was conducted in this bioreactor in order to produce lipase from agricultural products/residues by Aspergillus niger. Regarding this issue, several agricultural products and residues including rice bran, sugarcane baggase, wheat bran, barley bran and corn meal were employed. Among them rice bran led to maximum lipase production under similar condition. Furthermore the influence of process parameters including fermentation duration for lipase production, temperature and humidity of the cabinet were investigated. Analysis showed that, the best result for enzyme production was obtained after 96 hours of incubation, at cabin temperature of 35°C and cabin humidity of 90%. The maximum produced enzyme activity under desired conditions was142.732 U/gds.
Production of Lipase enzyme by Aspergillus flavus using Groundnut waste
The present study was carried out to determine the production of the lipase enzyme from Aspergillus flavus under submerged fermentation. Lipase containing protein content was estimated. Effect of H bio process variables such as p , temperature and substrate concentration of Lipase activity were also H monitored during lipase production. The enzyme was active at p 5, 32°c and 7gm substrate concentration in H submerged fermentation. The amount of enzyme produced at p 5 was 2.501IU/ml, temperature 32°c was 2.380 IU/ml, 7gm substrate concentration was 2.358 IU/ml.
ABSTRACT Extracellular lipase production by Penicillium chrysogenum, Trichoderma harzianum and Aspergillus flavus was carried out through solid state fermentation using agro-industrial residues as substrates. For all three strains, the growth temperature was 29±1 °C, and 65 % w (g/gds) moisture content. The effect of three factors on lipase production rate was investigated: initial pH (6.0 and 7.0), time of fermentation (72 h, 96 h and 120 h), and type of mixed substrate (wheat bran-olive oil, and wheat bran-castor oil cake). The process was optimized applying a mixed level factorial design. Fermentation time and pH were found to have positive effects on lipase production and secretion rates. However, the time effect was larger than initial pH. Type of substrate demonstrated minor effective importance than the other two factors, and Aspergillus flavus showed the larger lipase production among the three strains. Results indicated that the three fungal strains were able to grow and produce lipase in both culture mediums. The maximum lipase activity achieved was 121.35 U/gds by Aspergillus flavus, which was five and nine times the lipase produced by Trichoderma harzianum and P. chrysogenum respectively, at the same conditions. An initial neutral pH and 96 h of fermentation time were the optimum conditions for lipase production by Aspergillus flavus.
Egyptian Journal of Microbiology, 2017
T HE CURRENT research deals with optimization of the factors affecting lipase production under submerged culture system. The most efficient isolate R1 was identified depending on cultural and morphological characteristics together with 18S rRNA sequence as Rhizopus oryzae. Using one variable at a time, the maximum lipase activity (171.8 U/mL) was recorded in the presence of 1% fish-frying oil, mixture of peptone and yeast extract at pH 5 with 8% v/v of fungal inoculum after 4 days at 30°C. The screening of the most significant factors using Plackett-Burman design revealed that among ten variables, four, i.e. incubation temperature, inoculum size, incubation period and agitation speed, affected significantly (p-values ranged from 0.003 to 0.049) on the lipase activity. Optimization by using response surface methodology (RSM) through central composite design (CCD) resulted in the highest predicted lipase activity (216.2 U/mL) in which fermentation medium was inoculated with 8% inoculum size and incubated at 28°C under agitation speed of 150 rpm for 4 days.
2004
Of the 34 fungal species, isolated from a number of oily substrates, 9 exhibited lipase activity. AU 15, identified as Aspergillus sp., was found to be excellent lipase producer in submerged fermentation and was selected for solid-state fermentation (SSF). Among substrates like oil cakes of coconut, groundnut and sesame, wheat rawa, bombay rawa and soya beans (crushed), wheat rawa showed the highest lipase activity. The maximum enzyme yield (1934 U/g) was obtained with basal medium containing wheat rawa, olive oil and corn steep liquor, at 80% moisture content, pH 7.0 and 96 hrs incubation.
Production and optimization of lipase using Aspergillus niger MTCC 872 by solid-state fermentation
Bulletin of the National Research Centre
Background: Lipases are serine hydrolases that degrade triglycerides, an attribute that treasures wide applications in biodiesel production, detergent, chemical industries, etc. The most sought after the application is in the high quality and economical production of biodiesel under mild reaction conditions and simplified product separation. For the said application, fungal lipases are ideal catalysts that could effectively catalyze esterification and transesterification reactions with their specific ability to release fatty acids from 1, 3 positions of acylglycerols. Results: In the present work, to facilitate bulk synthesis, lipase production using Aspergillus niger MTCC 872 was studied by solid-state fermentation (SSF). The chosen fungal strain was evaluated for lipase production using a mixture of agroindustrial substrates viz. rice husk, cottonseed cake, and red gram husk in various combinations at flask level. Tri-substrate mixture (rice husk, cottonseed cake, and red gram husk) combined in the ratio of 2:1:1 has shown the maximum lipase activity 28.19 U/gds at optimum cultivation conditions of temperature 40°C, moisture content 75% (v/w), pH 6.0 and initial spore concentration of 5.4 million spores per mL. Further studies were performed for scale-up of lipase from flask level to lab scale using tray fermenter. Lipase activity was found to be 24.38 U/gds and 21.62 U/gds for 100 g and 1000 g substrate respectively. Conclusion: This is the first report on the production of lipase from Aspergillus niger MTCC 872 using tri-substrate mixture of rice husk (RH), cottonseed cake (CSC), and red gram husk (RGH). Moreover, comparison between individual, binary, and tri-substrate mixture was carried out for which the highest lipase activity was observed for tri-substrate mixture. In addition, comparable results were found when scale-up was performed using tray fermenter. Thus, the current work signifies usage of agro-industrial residues as substrates for enzyme production by solid-state fermentation process as an effective alternative to submerged fermentation for industrial applications.
AFRICAN JOURNAL OF BIOTECHNOLOGY
The current study evaluated lipase production by Aspergillus candidus URM 5611 through solid state fermentation (SSF) by using almond bran licuri as a new substrate. The microorganism produced high levels of the enzyme (395.105 U gds -1 ), thus surpassing those previously reported in the literature. The variable moisture content, the inductor concentration, temperature and pH were analyzed. The best production conditions were initial moisture content of 75%, 0% of inductor, at 25°C, pH 5.5, in 10.0 g of substrate. The variables initial moisture and inductor contents showed significant effects on lipase production by increasing enzyme activity in about 200% when compared with the initial screening (197.44 U gds -1 ). Optimum lipase activity was obtained at pH 2.5 at an optimum temperature of 65°C. The lipase was stable from pH 2.5 to 9.0 and at temperatures between 30 to 65°C. Results from the study are promising for the economic use and value addition from these important agro residues, which are abundantly available in Brazil. This is the first report on lipase production by A. candidus URM 5611 reaching a much higher yield of enzyme under SSF with almond bran licuri used as substrate.
Lipase production in solid state fermentation using aspergillus niger: Response surface methodology
Lipases are the most widely used biocatalysts, because they can catalyze several unnatural and remarkable reactions in non-aqueous media, such as bio-fuel production, production of value-added products such as esters, organic acids, food, beverage, cosmetics and pharmaceutical materials. Solid-state fermentation (SSF) represents an interesting alternative to produce industrial enzymes at lower costs due to the possibility of using inexpensive agro-industrial residues as culture media. This review aims to explore various agriculture byproducts like husk, straw, agricultural raw materials, waste of the oil industry, among others that are locally available and are also costeffective requiring low nutrient supplementation to produce microbial lipase(s) in SSF. Enzyme production is associated with the growth of the bacterial culture. The physico-chemical fermentation parameters such as pH of the medium, moisture content, particle-size, nature of particles and microbial inoculum level play crucial role(s) in lipase production. SSF has gained renewed interest and fresh attention of researchers to develop processes to achieve large-scale enzyme production by solid waste treatment and in its application in the industry to synthesize the products of commercial value.
AFRICAN JOURNAL OF BIOTECHNOLOGY, 2011
In this study, cultural conditions were optimized for enhanced production of lipase by Penicillium notatum. Different agricultural wastes including canola oilseed cake, sesame oilseed cake, linseed oil cake, cotton oilseed cake, rice bran and wheat bran were used as substrates for their potential to be used for the production of lipase under solid state fermentation conditions. Among these, the canola oil seed cake proved to be the best substrate for production of lipase under solid state fermentation conditions. A maximum lipase activity of 5335 U/g dry substrate was observed after 96 h of incubation period using 60% moisture content, at pH 5, incubation temperature of 30°C and olive oil of 3%. Enrichment with maltose as carbon source enhanced the lipase production significantly, while nitrogen supplementation did not affect the lipase production significantly. The optimization of conditions led to twofold enhancement of lipase activity as compared to the initial enzyme activity.