Inhibitory and Bactericidal Effects of Pseuderanthemum palatiferum and Piper betle L. Leaves Extracts Against Salmonella species (original) (raw)

Abstract

Objectives __ To study the inhibitory and bactericidal effects of Pseuderanthemum palatiferum and Piper betle L. leaves extracted by 3 solvents (distilled water, 40 degree liquor, and 95% ethanol) against Salmonella spp. isolated from humans and pigs. Materials and Methods __ Leaves of Pseuderanthemum palatiferum and Piper betle L. were extracted separately either by distilled water, 40 degree liquor, or 95% ethanol (each with ratio 1 gram of the leaf/20 ml of a solvent) for 12 hours. The extracts were then filtered and dried with 60 Ì C incubation. Dried extracts were dissolved in distilled water (ratio 250 mg/ml) before use. Eight strains of Salmonella spp. (5 from humans and 3 from pigs) were tested with the extracts by broth microdilution method for antimicrobial susceptibility. The experiments were done with 4 replicates. Results __ For Pseuderanthemum palatiferum leaves, minimal inhibiotry concentration (MIC) and mininmal bactericidal concentration (MBC) values of the distille...

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