Temporal Variation of Culture Independent Bacterial Diversity Based on Amplicon Sequencing of the North Sikkim Hot Spring Solfataric Soil Sediments (original) (raw)

The solfataric soil sediments of the hot springs of Sikkim located at Yume Samdung and Lachen valley were studied for deciphering the uncultured bacterial diversity. The main aim here is to present a comparative study and generate a baseline data on the post-monsoon seasonal variation for the months of October and December, analysed through 16S rRNA V3-V4 amplicon sequencing. At phylum level, in the New Yume Samdung (NYS) hot spring soil sample, in the month of October it was dominated by Proteobacteria (21.03%) followed by Thermi (12.33%) and Bacteroidetes (9.95%) whereas in December it was dominated by Proteobacteria (23.61%) followed by Chloroflexi (19.15%) and Bacteroidetes (16.55%). For Old Yume Samdung (OYS) hot spring soil sample, in the month of October, it was dominated by Proteobacteria (25.22%) followed by Bacteroidetes (11.76%) and Thermi (8.11%) whereas in December it was dominated by Proteobacteria (20.58%) followed by Chloroflexi (18.88%) and Thermi (9.89%). And in th...