In-vitro antioxidant activities of ocimum gratissimum, vitex doniana, carica papaya and peristrophe bicalyculata using DPPH free radical scavenging activity (original) (raw)
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International Journal of Biosciences, 2024
Medicinal plants have been explored for the presence of phytochemicals and natural antioxidants for research and commercial utilization potentials. However, few studies were conducted on the selected medicinal plants' phytochemical profile and antioxidant contents. This study investigated the phytochemical profile, total phenolic, and total flavonoid contents of five methanolic leaf extracts of C. igneus, A. bilimbi, M. charantia, A. esculentus, and B. glabra. All five plants indicated the presence of carbohydrates, saponins, and alkaloids. A. bilimbi, M. charantia, A. esculentus, and B. glabra are potential sources of tannins. The total phenolic content of the extracts varied from 10.17±0.23 to 27.90±0.50 GAE/g. Whereas, the total flavonoid content of these plants ranges from 8.40±0.52 to 87.48±2.42 QE/g. The M. charantia leaves revealed the highest TPC while A. bilimbi yielded the highest TFC among the plants. The selected medicinal plants can be considered good sources of phytochemicals and natural antioxidants, phenolics, and flavonoids, which are the basis for the development and utilization of these plant leaves. The phytochemicals, phenolics, and flavonoids of these plants could be further isolated, purified, characterized, and utilized as antioxidants.
Oxidants and Antioxidants in Medical Science, 2014
Objective: Reactive oxygen has been implicated in degenerative diseases and medicinal activity of most herbs has been attributed to their ability to scavenge free radicals. Methods: Free radical scavenging and antioxidant activities of ethanolic extracts from the leaves of varieties of medicinal plants, namely Azadiratcha indica, Mangifera indica, Moringa oleifera, Psidium guajava, Terminalia catappa, Anacardiaceae occidentale, Cassia siamae, Chromolaena odorata, Telfaira occidentalis and Paraquetina nigresiens were evaluated. Reducing power, DPPH scavenging, hydroxyl radical scavenging and ferrous ion chelating activities, phenolic, flavonoid and vitamin C contents were determined. Results: C.siamae had excellent DPPH scavenging activity while P.guajava presented the lowest value. T.catappa had peak hydroxyl radical scavenging activity whereas T.occidentalis had the least capacity. C.siamae had the highest total phenolic and flavonoid contents while P.guajava and P.nigresiens showed the lowest phenol and flavonoid values. C.siamae also exhibited the highest reducing power activity whereas P.nigresiens had the least value. P.nigresiens had excellent ferrous iron chelating capacity while C.odorata had only poor activity. The total phenolic and flavonoid contents were highly correlated with the DPPH scavenging and reducing power activities, respectively. M.oleifera had the highest vitamin C content while P.guajava was the least.. Conclusion: Different values were obtained for each parameter for the medicinal leaves and free radical scavenging activity could be attributed to total phenolic and total flavonoid content. Among the plants tested, Cassia siamae leaf extract consistently exhibited the highest antioxidant activity and seems to be a promising source of natural antioxidants.
A comparative study on the antioxidant activity of selected medicinal plant extracts
European Journal of Biotechnology and Bioscience, 2016
The ethanol and methanol extracts of, Prunus dulkis (Almond), Ficus racemosa (Audumbar), Kalanchoe pinnata (Panphutti), Ocimum gratissimum (Tulsi) were screened for their free radical scavenging property using ascorbic acid as standard antioxidant. Free radical sacvanging activity was studied using DPPH method. Highest antioxidant activity observed in Kalanchoe pinnata with 69.841% and 78.56% in ethanol and methanol extract respectively. In case of ethanol extract Ficus racemosa, and Ocimum gratissimum showed higher antioxidant activity as compared to other. In case of methanol extract Kalanchoe pinnata is at top. This present study proves that the plant extracts have considerable antioxidant property and further detailed studies and characterization of these plant extracts can contribute to their medicinal and pharmaceutical applications.
Evaluation of Phytochemicals and Antioxidant Activity in Medicinal Herbs
International Journal for Research in Applied Science and Engineering Technology (IJRASET), 2022
As the presence of excess free radicals in body can cause damage to the system and may lead to several diseases like Alzheimer's, Parkinson's, Cancer, Atherosclerosis and many more. Free radicals should be neutralized or eliminated out of the body. Apart from the antioxidants present in biological system natural antioxidants available from the diet help in the removal of free radicals from the body. Therefore, the antioxidants present in fruits, vegetables and medicinal herbs can be used to scavenge free radicals and treat the disorders due to oxidative stress. Five different medicinal herbs were collected locally to quantitate phytochemicals, antioxidant activity and inhibition of lipid, protein and sugar oxidation. Among the different samples, highest Phenolics, flavonoid and antioxidant activity was seen in aqueous extract of Ocimum basilicum. This study demonstrates that it can be used in nutraceuticals and can be a potential source of natural antioxidants to combat the free radical mediated diseases.
Screening of the Antioxidant Activity of Some Medicinal Plants
Korean Journal of Food Science and Technology, 2004
Antioxidant activities of water extracts of 20 medicinal plants (1 mg/mL) on peroxidation of linolic acid were evaluated by thiocyanate method, among which 11 showed strong antioxidant activity (> 70%). Higher hydroxy radical scavenging activity (> 60%) were shown in Cornus officinalis, Acanthopanax sessiliflorus, and Epimedium koreanum than the other plants. Epimedium koreanum than the other plants extract showed highest superoxide radical scavenging activity (42%). Total polyphenol contents ranged from 2.6 (Polygonatum odoratum)~81.2 (Epimedium koreanum) mg/g. Direct correlation between the antioxidant activity and polyphenol content (r=0.8) was established through simple regression analysis. IC 50 for selected four plant extracts, showing highest polyphenol contents and antioxidant activities, were significantly higher than positive control. Total antioxidant activity of vitamin c was significantly lower than those of Acanthopanax sessiliflorus, Epimedium koreanum, and Erythrina variegata. Superoxide radical scavenging activity of Acanthopanax sessiliflorus was similar to BHA. Results suggest water extracts of some medicinal plants could be potential candidates for natural antioxidants.
A review of the antioxidant potential of medicinal plant species
Food and Bioproducts Processing, 2011
Some researchers suggest that two-thirds of the world's plant species have medicinal value; in particular, many medicinal plants have great antioxidant potential. Antioxidants reduce the oxidative stress in cells and are therefore useful in the treatment of many human diseases, including cancer, cardiovascular diseases and inflammatory diseases. This paper reviews the antioxidant potential of extracts from the stems, roots, bark, leaves, fruits and seeds of several important medicinal species. Synthetic antioxidants such as butylated hydroxytoluene (BHT) and butylated hydroxylanisole (BHA) are currently used as food additives, and many plant species have similar antioxidant potentials as these synthetics. These species include Diospyros abyssinica, Pistacia lentiscus, Geranium sanguineum L., Sargentodoxa cuneata Rehd. Et Wils, Polyalthia cerasoides (Roxb.) Bedd, Crataeva nurvala Buch-Ham., Acacia auriculiformis A. Cunn, Teucrium polium L., Dracocephalum moldavica L., Urtica dioica L., Ficus microcarpa L. fil., Bidens pilosa Linn. Radiata, Leea indica, the Lamiaceae species, Uncaria tomentosa (Willd.) DC, Salvia officinalis L., Momordica Charantia L., Rheum ribes
Screening of medicinal plant extracts for antioxidant activity
Journal of Medicinal Plants …, 2009
The methanolic crude extracts of Desmodium gangeticum (Linn.), Eclipta alba (Linn.) Ocimum sanctum (Linn.), Piper longum (Linn.), Solanum nigrum (Linn.) and Amaranthus caudatus (Linn.) were screened for their free radical scavenging properties using ascorbic acid as standard antioxidant. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picryl hydrazyl (DPPH) free radical. The overall antioxidant activity of D. gangeticum was found to be the strongest, followed in descending order by A. caudatus, S. nigrum, P. longum, E. alba and O. sanctum. The IC 50 values of the extracts ranged between 0.05 ± 0 and 0.19 ± 0 mg/l . The ascorbic acid levels varied from 3.86 ± 0.20 to 21.33 ± 1.49 mg/100g and the carotenoids content were observed between 9.0 ± 0.24 to 24 ± 1.16 mg/100g in plant extracts. The highest total phenols content were found to be in O. sanctum (Linn.) with the value 48.93 ± 0.24 mg/g. The present study reveals that the selected plants would exert several beneficial effects by virtue of their antioxidant activity and could be harnessed as drug formulation.
Comparative Analysis of Antioxidant Properties of Different Medicinal and Non-Medicinal Plants.
The crude methanolic leaf extracts of Artemisia annua, Withania somnifera and Brassica juncea was studied to evaluate and compare their antioxidant activity by the using in-vitro methods. The extracts was found to contain significant amount of phenols and had high antioxidant potential as found in Folin- Ciocalteau assay, DPPH free radical scavenging assay and β-Carotene bleaching assay. The total phenolic contents of the extracts varied from 53.7 mg/gm in Brassica juncea to 69.34mg/gm in Withania somnifera and 72.32mg/gm in Artemisia annua. The DPPH free radical scavenging activity shows that Withania somnifera has 78.18% scavenging effect while Artemisia annua and Brassica juncea showed 66.7% and 56.36 %respectively. The β-carotene bleaching of these plant extracts showed the presence of significant amounts of antioxidants. The total antioxidant content found in the leaf extracts was found to be 193.6 mg/gm dry weight in Artemisia annua, 205mg/gm dry weight in Withania somnifera and 78.18mg/gm dry weight in Brassica juncea.
Study of eight medicinal plants for antioxidant activities
There is now an expansion of interest in phytochemicals as a new source of natural antioxidants to be used in foods and pharmaceutical preparations to substitute synthetic antioxidants, which are being restricted due to their potential health risks and toxicity. Eight Libyan medicinal plants belonging to different families were extracted successively with three solvents of different polarities using microwave technique. The antioxidant activity of these plant extracts were evaluated using 2,2, Diphenyl-1-picryhydrazyl (DPPH) radical scavenging method. Among the twenty four crude extracts, six showed strong antioxidant activity (IC 50 ranging between 4.55 to 21.55 µg/ml), six of the extracts exhibited moderate antioxidant activity (IC 50 values ranged between 40.7 to 94.4 µg/ml), and four crude extracts exhibited weak antioxidant activity (IC 50 more than 200 µg/ml). The IC 50 values of quercetin and ascorbic acid, used as standards in this assay were found to be 3.35 and 15.35 µg/ml...