Molecular detection of some genes virulent bacteria Proteus mirabilis isolated from kidney stones (original) (raw)
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Identification of probiotic bacteria in aqueous extract from Mazafatit, Piarom and Zahedi
Cellular and Molecular Research#R##N#(Iranian Journal of Biology), 2021
Introduction: The beneficial effects of probiotic bacteria on human and animal health has been widely studied and confirmed in various cases. To obtain probiotic bacteria, Bacillus and lactic acid bacteria, dates were considered as one of the important nutrients that could be the source of isolation of these bacteria. Aim: The aim of this study was to isolate probiotic bacteria of lactic acid bacteria and Bacillus from aqueous extract of Mazafati, Pourm and Zahedi. Methods: For isolation of lactic acid bacteria and Bacillus, the extract was grown on MRS agar and agar nutrient. After 5 days of incubation at 37 ° C under aerobic and anaerobic conditions, colonies isolated with morphological characteristics and microscopic form associated including positive gram bacteria. positive and negative catalase bacteria isolated from MRS agar and nutrient agar, respectively. These bacteria were evaluated using biochemical and polymerase chain reactions. Results: According to chemical and molecular tests, Lecunostoc mesenteroeides subsp mesenteroeides, Bacillus subtilis strain UD1022 and Pediococcus parvalus strainSC8B isolated from Mazafati, Piarom and Zahedi date extract . All three bacteria were probiotic. Discussion and Conclusion: According to the results of the experiments, dates are in the probiotic collection. Aqueous extract of dates can be used to enrichment and preparing of probiotic foods. Also, according to the characteristics of probiotic bacteria, aqueous extract of dates or dates may be recommended to prevent of pathogenic bacteria replacement and disease for consumers.
عزل وتشخيص بكتريا sp. Pseudomonas المنتجة لأنزيم اللايبيز المحلل للدهون
iraq journal of market research and consumer protection, 2019
15 local isolates of Pseudomonas were obtained from 35 samples from several sources such as soil, water and some high-fat foods. The ability of isolates to produce lipase was measured by the size of the clarification zone formed around the colonies on the lipase production medium and by measuring the enzymatic activity and specific enzymatic activity, the isolate M3 was found to be the most efficient for production of the enzyme, This isolate was identified by microscopic, morphological, some biochemical tests and genetic diagnosis of 16S gene sequences by using the (PCR) technique, and then comparing the results obtained with the National Center for Biotechnology Information (NCBI) website, M3 isolates were found to be Pseudomonas aeruginosa .
Tikrit Journal of Pure Science
The number of people who wear contact lenses (CLS) has been especially increased in the past decade as an alternative to glasses, but many of them do not realize the extent of the hazards involved, and these risks Keratitis associated with microbial causes, so this study included isolation and identification the bacteria in fluids save to lenses with 42 users of contact lenses of students in inner section in the Tikrit University. The study showed the presence of different bacterial groups in the fluids keeping contact lenses which included Staph. epidermis with percentage 30.30% and Staph. Aureuswith percentage 21.21% and the bacteria Streptococcus pneumonia precentage 18.18% and also was diagnosed with Pseudomonas aeruginosa and with 9.09%, while the intestinal bacteria Serratia odorfera where it was obtained with 6.06%. As for the sensitivity of the bacterial isolates to antibiotics, the study proved that more antibiotic effect on gram positive and negative bacteria is erythrom...
Objective: The aim of the this study was to investigate the prevalence of toxB, paa, lpf and iha adhesion genes in enteropathogenic Escherichia coli (EPEC) isolates lacking in two important adhesion factors, the eaeA and bfpA genes. Methods: We examined a total of 70 serologically confirmed EPEC (eaeA-, bfpA-) isolates. DNA from the isolates was extracted by the phenol-chloroform method. toxB, paa, lpf and iha adhesion genes in the EPEC isolates were detected by polymerase chain reaction. Data were analyzed by SPSS software and statistical analysis using the chi square test. P-values less than 0.05 were considered significant. Results: PCR was positive for the toxB gene in 2 (2.85%), paa in 3 (4.28%), lpf in 32 (45.71%) and iha in 15 (21.42%) of the 70 strains. Statistically, none of the toxB, paa, and lpf genes were associated with diarrhea. However, the iha gene showed a weak significant relation to diarrhea (P=0.11). Conclusion: The main mechanism of pathogenicity for EPEC is attachment and effacement. Therefore, EPEC (eaeA-, bfpA-) should have another adhesin factor, which should be investigated. EPEC strains (eaeA-, bfpA-) that possess the lpf gene are common. Further investigations of the virulence properties of these strains are necessary in order to elucidate the role of these virulence factors in diarrhea among Iranian children.
علوم و صنایع غذایی, 2021
The toxic effects of synthetic antimicrobial and antioxidants compounds have led to an increase in the use of natural types, such as plant extracts. In this study, the antimicrobial effect of Lawsonia inermis aqueous extract was investigated against Escherichia coli, Salmonella typhimurium, Staphylococcus epidermidis, and Listeria innocua, by disc diffusion agar, well diffusion agar, minimum inhibitory concentration, and minimum bactericidal concentration methods. The higher concentrations of the extract showed higher antimicrobial effects against S. epidermidis and L. innocua compared to E. coli and S. typhimurium. Moreover, the total phenolic and flavonoids contents of the extract were 59.45±1.12 mg gallic acid per gram of extract and 40.35±0.70 mg quercetin per gram of extract, respectively. The antioxidant properties of aqueous extract were measured based on the DPPH and ABTS free radical scavenging activities. It was found that the antioxidant activity increased significantly as a function of L. inermis aqueous extract concentration. The antimicrobial and antioxidant properties of L. inermis leaf aqueous extract could make it a natural bioactive compound to be used.
2014
According to the white spot disease during 2002 until 2005 and the damages caused by the disease to the farmers a new species L.vannamei were introduced to the farming region of Abadan Choebdeh from 2006. Hight mortality occurred in Khuzestan province farms in 2007 and 2008 again. Probably viral agent was transferred to native shrimps or other aquatic animals, therefore it is very important that presence of viruses in local aquatic animals and its vectors detected. Detection of WSSV, TSVand IHHNV in aquatic animal from Khuzestan costal region imphasis to wild shrimp and craps is the main objectives of this study. So samples were taken from 10 active farms twice a week and 100 postlarves from 3 active breeding center befor stocking. Samples for viral detection were studied by molecular and histopathology asseys. Results was shown presence of the White spot virus in cultured and wild shrimp and TSV infection in only two cultured shrimps but there was negative result for IHHN virus. To...
Identification of facultative heterofermentative Lactobacillus species in Lighvan cheese
Iranian Food Science and Technology Research Journal, 2010
Facultative Heterofermentative Lactobacilli (FHL) are responsible for enhancing unique flavour properties in traditional dairy products such as Lighvan cheese through biochemical mechanisms. In addition to saving domestic genetic resources, industrial production of semi-traditional dairy products is one of the major objectives of non starter microflora identification in indigenous dairy products. In this study performed on the Lighvan cheese at 4 months ripening period Based on morphologicaland biochemical methods were identified Lb. plantarum group (Lb.pentosus, Lb. plantarum, Lb. paraplantarum) and Lb. casei group ( Lb. casei, Lb. paracasei) .Moreove the development of the FHL in Lighvan cheese varied according to ripening time. Keywords: Lighvan cheese, Lactobacillus plantarum, Lactobacillus casei
Arak Medical University Journal, 2013
Background: Anthrax is a common disease among human and livestock which is caused by Bacillus anthracis. Bacillus anthracis has two strong immunogenic proteins: Protective antigen (PA) and lethal factor domain I (LFD1) that have always been considered as vaccine candidates against Bacillus anthracis. The aim of this study is to express and purify the lethal factor domain I (LFD1) in Escherichia coli and produce polyclonal antibody against it in mice. Materials and Methods: In this experimental study, LFD1 gene was amplified with BamH I and Xho I restriction site by PCR. After isolation, the gene was cloned to the expression vector pET28a (+). This vector was transformed to E. coli-BL21 (DE3) PLysSto to express LFD1 gene. The expression of LFD1 gene was induced by IPTG. After protein purification by affinity chromatography, the produced antigen was injected into mice for four times. Then the produced polyclonal antibody in mice serum was evaluated. Results: The cloned LFD1 gene in pET28a (+) vector was confirmed by PCR, enzymatic analysis, and sequencing. The expressed and purified recombinant protein was confirmed by SDS-PAGE and Western blotting. Finally, the isolated polyclonal antibody from mice serum was evaluated and confirmed by ELISA test. Conclusion: Noticing the appropriate expression, easy purification of LFD1, and the titer of produced polyclonal antibody against LFD1 in mice due to its immunogenicity, it can be considered as a good vaccine candidate against anthrax.
Maǧallaẗ ǧāmiʻaẗ kirkūk, 2019
The study included, the collection of (250) swabs which involve (180) samples from general children's Hospital environment and various isolation sources, In addition to (70) smear from the hands and noses of medical staffs and workers, Using sterile cotton swabs and then planted on the Cultuer media (MacConkey's agar and Blood agar). The results showed that (126) smears and in a ratio of (50.4%) was positive for the bacterial isolations which contribute the followings:-Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli , Enterobacter cloacae, Proteus mirabilis, Klebsiella pneumonia, Pseudomonas aeruginosa, with variant isolation ratios. It high rate of contamination (73.33%), was from Hospitals environment compared to (71%) of contamination from the hands of medical staffs. Resistany agaings antibiotic was praceddon (12) antibiotics and the results showed that most of the isolates are resistant For anti-beta-lactam groups the rate was (81.74%), the followings antibiotics vancomycin, imipenem, amikacin reveal high efficucy against the isolated Staph.aureus , Staph.epidermidis , Esch.col, Prot.mirabilis , pathogens Ps.aeruginosa with reduction resistany reach to (23.80), (24.60), (36.50) respectively. The Minmam inhibitory concentration was detected using virkon, Opizil, Strillium, and bleach, ethyl alcohol, Optisal ® N was found to passing a better inhibition effect on the elected isolates, followed by an Virkon, compared with the disinfectant of sodium hyper chlorate (minor), The