BIOGENIC SYNTHESIS SILVER NANOPARTICLES USING PELTOPHORUM PTEROCARPUM LEAF EXTRACTS AND ITS ANTIMICROBIAL EFFICACY AGAINST SELECTIVE PATHOGENS Original Article (original) (raw)
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Green synthesis is one of the rapid, reliable, and best routes for the synthesis of silver nanoparticles (Ag NPs). The current study revealed that the aqueous leaf extract of Pterocarpus santalinus, which contains steroids, saponins, tannins, phenols, triterpenoids, flavonoids, glycosides, and glycerides, is found to be responsible for bioreduction during the synthesis of spherical Ag nanoparticles. The formed Ag NPs were characterized by ultraviolet-visible (UV-vis), Fourier transform-infrared (FT-IR), X-ray diffraction (XRD), atomic force microscopy (AFM), energy-dispersive X-ray spectroscopy (EDX), and scanning electron microscopy (SEM) analysis. UV-vis spectra of the aqueous medium containing silver nanoparticles showed a surface plasmon resonance peak at 418 nm. FT-IR analysis was performed to analyze the biomolecules responsible for the reduction of Ag NPs. XRD results confirmed the presence of silver nanoparticles with face-centered cubic structure. The EDX analysis showed the completed inorganic composition of the synthesized Ag NPs. AFM analysis exemplified the results of particle sizes (41 nm). The calculated crystallite sizes are in the range of 20 to 50 nm, and the spherical nature of the Ag NPs was ascertained by SEM. The synthesized Ag NPs exhibited good antibacterial potential against gram-positive and gram-negative bacterial strains. The zone of inhibition effect of antibacterial activity depends upon the concentration of Ag NPs.
Journal of the Mexican Chemical Society
The synthesis and applications of nanomaterials are an excitingand emerging field of scientific research. In this paper, silver nanoparticles (AgNPs) were synthesized using parsley leaf extract via the screening method (autoclave hydrothermal heating, and heater), and their microbial activities were studied. An extract of chopped parsley leaves was prepared. AgNO 3 salt with a concentration of 1 mM was used. In every two methods of autoclave hydrothermal heating and heater, 5 mL of the Parsley leaf extract was mixed with 25 mL of silver nitrate salt. By FTIR analysis, the presence of Petroselinum crispum (parsley) leaf extract in NPs was identified. By using a hydrothermal autoclave and heater to synthesize AgNPs at the lowest wavelength, the size of NPs was achieved at 403 nm and 414 nm, respectively. All samples synthesized by different techniques had suitable zeta potential and acceptable stability. By giving the zeta potential of NPs synthesized by autoclave (-14.3 mV), results showed that this method is suitable for the production of AgNPs. Comparing the antibacterial attributes of the synthesized Ag NPs by different techniques (autoclave and heater) on Gramnegative bacteria (E. coli) showed 16±1, and 12±1, respectively.
Journal of Bionanoscience, 2017
Objectives: The main objective of the present study was to synthesize silver nanoparticles (AgNPs) by green approach using Helvella leucopus and to evaluate the antimycotic activity of synthesized AgNPs against fungi causing fungal rot of apple. Methods: During the present study for green synthesis of AgNPs using H. leucopus, equal volumes of both mushroom extract (100 ml) and silver nitrate solution (100 ml) were mixed and incubated at room temperature for the bioreduction process. These synthesized AgNPs were characterized by ultraviolet-visible spectroscopy, scanning electron microscopy, Fourier transmission infrared spectroscopy, and X-ray diffraction analysis. Furthermore, these synthesized AgNPs were evaluated for their antimycotic activity by spore germination method and agar well diffusion assay against different tested fungi. Results: The results revealed that strong plasmon absorbance band was observed at 420 nm which confirms the synthesis of AgNPs using H. leucopus. The synthesized AgNPs were spherical in aggregated form with size ranging from 80 to 100 nm. Furthermore, different concentrations of synthesized AgNPs caused significant inhibition in spore germination and reduction in zone of inhibition of tested fungal pathogens. The highest inhibition in spore germination by AgNPs at highest concentrations was observed against Penicillium chrysogenum followed by Aspergillus niger and Alternaria alternata, respectively. Similarly, the synthesized AgNPs at highest concentrations showed maximum zone of inhibition against P. chrysogenum followed by A. niger and A. alternata, respectively. Conclusion: It is concluded from the present study that synthesized AgNPs have good potential to be used as antifungal agents against many fungal plant pathogens. The synthesized AgNPs using mushroom fungi also have potential for the development of nanofungicides against fungal pathogens but after proper investigation.
Jurnal Teknologi Laboratorium, 2021
This research aims to develop a silver nanoparticle (AgNPs) using several plant extracts for antibacterial application. The papaya seed extract has been fractioned by n-hexane, ethyl acetate, water, and ethanol. The n-hexane fraction was the only fraction that succeeded in synthesizing AgNPs. The characterization methods showed AgNPs marked at 430 nm with UV-Vis and 1640 cm-1 with FTIR. SEM observed the aggregation of spherical AgNPs at the 200 nm scale. The particle size of 91.3 nm was measured with PSA that confirmed the nanoscale of the synthesized material. All fractions contained alkaloid compound, and ethyl acetate fraction showed a group of indole with specific wavenumber at 2623 cm-1 , 1737 cm-1 , and 1237 cm-1 representing N-H, C=O, C-N, respectively. All fractions at every concentration (25%, 50%, 75%, 100%) have been tested and showed the medium effect on bacterial growth inhibition. Among all fractions, the AgNPs n-hexane fraction has the highest bacterial effect, which was indicated by mean values of inhibition zone 7.2 mm against S.aureus, as well as 6.6 mm against E.coli. ANOVA analysis showed that AgNPs n-hexane fraction has a significant inhibition zone compared to other fractions against S.aureus (p=0.002), but not significant to E.coli (p=0.128). The insignificant results on E.coli because of gram-negative bacteria's biophysical characteristics, such as membrane cell wall and flagellin. This research emphasized that AgNPs could be synthesized via a green process of nucleation by using plant extract that effectively inhibits the growth of S.aureus and E.coli. Further studies on the mechanism of the antibacterial effect at the molecular level might be investigated soon.
Antibacterial activity of biogenic silver nanoparticles was evaluated by zone of inhibition using standard agar disc diffusion method. Among three different concentrations (25, 50 and 100 µl) 100 µl concentration showed maximum activity against Klebsiella pneumonia (2.7 cm), Shigella someneii (2.4 cm), S. flexaneri (2.4 cm), Pseudomonas aeruginosa (2.2 cm), P. mirabilis (2.1 cm) and E. coli (2.0 cm) when compared to the other two concentrations. It was also observed that it showed no activity against geranium plant extract that served as control.
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Development of cost-effective and eco-friendly methods of nanoparticle synthesis could play a crucial role in integrating nanotechnology and phytomedicine for biological applications. In this study, biogenic silver nanoparticles (AgNPs) were synthesized using the ethanolic extract of Pelargonium sidoides DC at 60°C. Formation of nanoparticles was monitored using UV-Visible spectroscopy at different time intervals. A maximum absorption at 456 nm was observed as the reaction time increased, resulting in a red shift of the surface plasmon band (SPB). Attenuated total reflectance Fourier transform infrared spectroscopy (FTIR) revealed the reducing and stabilizing activity of flavonoids, coumarins, tannins, and phenols. Size and morphology of the AgNPs were analysed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) which indicated the spherical nature of the nanoparticles with sizes ranging from 11 to 90 nm. Further characterization of the AgNPs was carr...
International Journal of Applied Sciences and Biotechnology, 2018
The green route of metal nanoparticles synthesis has received significant attention in recent years due to it's cost-effective, non-toxic and eco-friendly nature in comparison to other physical and chemical methods. This study reports on the synthesis of silver nanoparticles (Ag-NPs) from bio-reduction of 1mM aqueous silver nitrate (AgNO3) by extracts prepared from three different plants namely, Brassica oleracea L. var. italica Plenck (Broccoli), Capsicum annuum L. (Chili) and Parthenium hysterophorus L. (Carrot grass). The synthesized Ag-NPs were characterized using UV-visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Ag-NPs synthesized showed the surface plasmon resonance with the appearance of absorption peaks around the range of 410-430 nm. The possible biomolecules involved in the reduction and the stabilization of synthesized Ag-NPs were found to be alcoholic, phenolic, amine and carbonyl groups. SEM study revealed that Ag-NPs were spherical in shape with varied size about 10-40 nm. Besides, the analysis of antioxidant and antibacterial activities of Ag-NPs was carried out. The Ag-NPs synthesized using B. oleracea extract showed the higher antioxidant activity than Ag-NPs synthesized from both C. annuum & P. hysterophorus extracts. Ag-NPs exhibited good antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. The higher antibacterial activity was shown by Ag-NPs synthesized from P. hysterophorus extract in comparison to Ag-NPs synthesized from both C. annuum & B. oleracea extracts. Hence, it can be concluded that Ag-NPs synthesized following the green route could be the source for potential antioxidant and antibacterial agents.
2019
Nanotechnology is a rapidly developing field, the purpose of which includes manufacturing new materials at nano scale. The current technique of green synthesis of silver nanoparticles (AgNPs) is a progressive method used in medical science. An emerging problem for medical sector is multi drug resistance to antibiotics which is shown by wide range of bacteria pathogens. This study investigates an efficient and sustainable route of AgNPs preparation from 1mM aqueous AgNO3 using leaf extracts of seven plants Trachyspermum ammi (ajwain), Moringa oleifera (drumstick), Phyllanthus niruri (nela usiri), Tagetes erecta (marigold), Pimenta dioica (jamaica pepper), Hemigraphis alternata (red ivy) and Plectranthus purpuratus (vicks) well adorned for their wide availability and medicinal property. AgNPs were synthesized by the reaction of 1mM AgNO 3 and 10% leaf extract of type of leaf separately. The solutions of AgNPs produced from plant extract were tested for its antibacterial activity. The ...
Detection the Antimicrobial Activity of Agnps Synthesized by Quercus Infectoria Plant
2020
Antimicrobial activity of plant extract of Quercus infectoria gall plant and silver nanoparticles (AgNPs) synthesized by Quercus infectoria galls with hot plat stirrer at 30 minutes. Silver nanoparticles were characterized using UV-visible, X-Ray, and SEM. Antimicrobial activity was determined by well diffusion methods against some pathogenic microbes (Staphylococcus aureus, E. coli, Pseudomonas aeruginosa, and Candida albicans). Biosynthesized silver nanoparticles were observed that the greatest effect on the growth of all pathogenic microbes compared with plant extracts. Also the synergistic effect of plant extract and silver nanoparticles with antibiotics was elevated. Key word : Antibiotics test; Green synthesis; Medicinal plant; Synergistic effect.
Eco friendly synthesis of AgNPs using Green apple and pepper extracts for antibacterial application
PROCEEDINGS OF THE III INTERNATIONAL CONFERENCE ON ADVANCED TECHNOLOGIES IN MATERIALS SCIENCE, MECHANICAL AND AUTOMATION ENGINEERING: MIP: Engineering-III – 2021, 2021
Silver nanoparticles AgNPs were synthesis by green method through the reduction of aqueous silver nitrate (AgNO 3) using apple and pepper (Malusviridis and capsicum annuum) peels extract as a reducing agent. The manufactured AgNPs were characterized by different instrumental techniques including energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), Fourier transformed infrared spectroscopy (FTIR), ultraviolet-visible spectrophotometer (UV-Vis), and field emission scanning electron microscopy (FESEM). The antibacterial behavior of Ag nanoparticles against Gram-negative (E. Coli) and Gram-positive (Staph. aureus) have been investigated. XRD analysis confirmed the single crystalline and polycrystalline structure for AgNPs. FTIR investigation carried out to identify possible biomolecules responsible in bioreduction of silver ions. The UV-visible spectrum showed an absorption peak at 415 nm and 460 nm, which reflects surface plasmon resonance (SPR) of AgNPs. FESEM images showed that nanoparticles had spherical morphology. The AgNPs for both plants extract showed to have antibacterial activity toward Gram positive and negative strains.