Supplementary Figure 1 from Chimeric Antigen Receptor T Cells with Dissociated Signaling Domains Exhibit Focused Antitumor Activity with Reduced Potential for Toxicity In Vivo (original) (raw)

Abstract

PDF file - 1125K, Figure S1. Correlated scFv and GFP transgene expression following F-28 CAR transduction. T cells were lentivirally transduced to express the costimulatory F-28 CAR and 5 days later were stained for surface scFv using biotinylated protein-L followed by SA-APC. Transduction efficiency was also monitored using GFP transgene expression. Transduction efficiencies are indicated with the percentage of CAR expression of the transduced populations. Coordinate expression of surface scFv and GFP was observed in all experiments.

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