Transcription of globin genes in reticulocyte chromatin (original) (raw)

This paper investigates the transcriptional activity of globin genes in reticulocyte chromatin, challenging traditional assumptions about chromatin conformation and transcriptional specificity. The findings indicate minimal specificity for globin gene transcription when using reconstituted chromatin, implying that the common methodologies for assessing transcription fidelity may not accurately reflect native chromatin behavior. The study employs various methods to assess RNA synthesis specificity and highlights concerns regarding the reliability of in vitro transcription analysis.